Rmu_ssc0000022.1_g000046

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000022.1
OG0016587
Physical Location & Seq
Reverse (-)
236947 .. 237855
909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000022.1_g000046.1.cds

Sequence Viewer

Length: 909 bp
atgaaagaagaccagaagaaatacacagctttactgaaactagcaaaacccttggtctttgtcatcctcctcctcttcacctacaccttaggctacctctcttctcctctaaccccctccccgacgtcaccctcaccaccctcttcttcagccccctcgcttcttcaactggccaacctccccacccaactcgaccacttccgagtcacaactcactgcgccgacccggtcccttcccaactcgtccgccaaaccattctcgacctagccttcaactccacctcgcctttcaccaacttccctcctccccacgtcaaaagcctcctacgtaccaatcgtgccaagggattgggcccaaacggcgccgttttcgagcacctaatcgaaatggtcaagccccggaccatcatcgaagtgggcacgtttcttggagcatccgctatccacatggtcgacttgacccgcaagctcgggctccaaacccaaatcctatgtgtcgatgactttcggggatggcccgggtttggagaacggttcaaggacataaagatgctaaacggcgacgtttttttgatgtaccaattcatgcaaaatatcgtatccgccaacgcgacggagtcggttatacccgtaccgttttcaaccgggtcggtacttgataagctttgtgagtggggtatttttggtgatttgatagaagtggatgcgggttatgattttaattcggcttggtcggatatcaaccgggcttatcggatcctaaaacccggtggggtgcttttcgggcacaattatttcaccgcggctgatgatagaggggtaagaagggcggtaactttatttgctagggtttatgggctcaaaattaaacttgaaaggcagcattgggtcattcactcgggttattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_24

Protein Analysis

302

Amino Acids

33.64

Weight (kDa)

9.05

Isoelectric Point (pI)

33.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016587)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01180
malus_domestica MD07G1156900.v1.1
prunus_persica Prupe.2G195400_v2.0.a1
pyrus_communis pycom07g15350
rosa_chinensis RchiOBHm_Chr1g0360821 RchiOBHm_Chr1g0360831
rosa_laevigata RLG00000027783
rosa_multiflora Rmu_ssc0000022.1_g000046
rosa_roxburghii Rroxscaffold_4G00295780
rosa_rugosa Rorug01G0281900
rosa_samantha Rh1AG293800 Rh1BG258600 Rh1DG288800
rosa_wichuraiana Rw1G026000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 362
AccI GTMKAC 1 cut(s) 453
AccII CGCG 2 cut(s) 611, 803
AciI CCGC 8 cut(s) 247, 438, 463, 603, 707, 801, 803, 830
AclWI GGATC 2 cut(s) 751, 764
AcoI YGGCCR 1 cut(s) 171
AcuI CTGAAG 1 cut(s) 132
AcyI GRCGYC 2 cut(s) 125, 363
AfaI GTAC 4 cut(s) 331, 578, 633, 654
AfiI CCNNNNNNNGG 1 cut(s) 524
AgsI TTSAA 5 cut(s) 167, 274, 538, 642, 875
AjiI CACGTC 1 cut(s) 313
AluBI AGCT 3 cut(s) 29, 469, 664
AluI AGCT 3 cut(s) 29, 469, 664
Alw21I GWGCWC 1 cut(s) 378
AlwI GGATC 2 cut(s) 751, 764
Ama87I CYCGRG 3 cut(s) 470, 518, 898
AoxI GGCC 3 cut(s) 171, 352, 515
ApaI GGGCCC 1 cut(s) 356
ApeKI GCWGC 1 cut(s) 880
AspLEI GCGC 2 cut(s) 221, 365
AspS9I GGNCC 5 cut(s) 229, 352, 353, 402, 516
AsuC2I CCSGG 7 cut(s) 227, 400, 519, 520, 646, 746, 768
AsuHPI GGTGA 6 cut(s) 70, 120, 126, 283, 698, 790
AvaI CYCGRG 3 cut(s) 470, 518, 898
AvaII GGWCC 2 cut(s) 229, 402
AxyI CCTNAGG 1 cut(s) 88
BaeGI GKGCMC 3 cut(s) 356, 422, 789
BalI TGGCCA 1 cut(s) 173
BamHI GGATCC 1 cut(s) 756
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 3 cut(s) 356, 477, 861
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 1 cut(s) 892
BccI CCATC 2 cut(s) 413, 507
BceAI ACGGC 3 cut(s) 350, 376, 574
BciVI GTATCC 1 cut(s) 610
BcnI CCSGG 7 cut(s) 227, 400, 519, 520, 646, 746, 768
BfaI CTAG 3 cut(s) 41, 266, 846
BfoI RGCGCY 1 cut(s) 366
BfuI GTATCC 1 cut(s) 610
BglI GCCNNNNNGGC 1 cut(s) 360
BisI GCNGC 2 cut(s) 804, 881
BlsI GCNGC 2 cut(s) 805, 882
Bme1390I CCNGG 7 cut(s) 227, 400, 519, 520, 646, 746, 768
Bme18I GGWCC 2 cut(s) 229, 402
BmeT110I CYCGRG 3 cut(s) 470, 518, 898
BmgBI CACGTC 1 cut(s) 313
BmgT120I GGNCC 5 cut(s) 229, 352, 353, 402, 516
BmiI GGNNCC 5 cut(s) 231, 354, 364, 476, 758
BmrFI CCNGG 7 cut(s) 227, 400, 519, 520, 646, 746, 768
BmsI GCATC 3 cut(s) 443, 540, 694
BpiI GAAGAC 1 cut(s) 15
BpuMI CCSGG 7 cut(s) 227, 400, 519, 520, 646, 746, 768
BsaAI YACGTR 1 cut(s) 329
BsaHI GRCGYC 2 cut(s) 125, 363
BsaJI CCNNGG 5 cut(s) 51, 342, 398, 518, 801
Bsc4I CCNNNNNNNGG 1 cut(s) 524
Bse1I ACTGG 1 cut(s) 174
Bse21I CCTNAGG 1 cut(s) 88
BseDI CCNNGG 5 cut(s) 51, 342, 398, 518, 801
BseGI GGATG 4 cut(s) 63, 434, 518, 709
BseLI CCNNNNNNNGG 1 cut(s) 524
BseNI ACTGG 1 cut(s) 174
BseRI GAGGAG 4 cut(s) 59, 62, 96, 294
BseSI GKGCMC 3 cut(s) 356, 422, 789
BseXI GCAGC 1 cut(s) 892
Bsh1236I CGCG 2 cut(s) 611, 803
BshFI GGCC 3 cut(s) 173, 354, 517
BshNI GGYRCC 1 cut(s) 362
BsiHKAI GWGCWC 1 cut(s) 378
BsiHKCI CYCGRG 3 cut(s) 470, 518, 898
BsiSI CCGG 6 cut(s) 227, 400, 519, 645, 745, 768
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 1 cut(s) 524
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 3 cut(s) 173, 354, 517
BsoBI CYCGRG 3 cut(s) 470, 518, 898
Bsp120I GGGCCC 1 cut(s) 352
Bsp1286I GDGCHC 6 cut(s) 356, 378, 422, 477, 789, 861
Bsp143I GATC 1 cut(s) 756
BspACI CCGC 8 cut(s) 247, 438, 463, 603, 707, 801, 803, 830
BspANI GGCC 3 cut(s) 173, 354, 517
BspFNI CGCG 2 cut(s) 611, 803
BspLI GGNNCC 5 cut(s) 231, 354, 364, 476, 758
BspPI GGATC 2 cut(s) 751, 764
BspT107I GGYRCC 1 cut(s) 362
BsrI ACTGG 1 cut(s) 174
BssECI CCNNGG 5 cut(s) 51, 342, 398, 518, 801
BssMI GATC 1 cut(s) 756
BssNI GRCGYC 2 cut(s) 125, 363
BssT1I CCWWGG 2 cut(s) 51, 342
Bst4CI ACNGT 2 cut(s) 534, 636
Bst6I CTCTTC 3 cut(s) 80, 106, 148
BstACI GRCGYC 2 cut(s) 125, 363
BstBAI YACGTR 1 cut(s) 329
BstC8I GCNNGC 1 cut(s) 467
BstDEI CTNAG 1 cut(s) 88
BstDSI CCRYGG 1 cut(s) 801
BstF5I GGATG 4 cut(s) 63, 434, 518, 709
BstFNI CGCG 2 cut(s) 611, 803
BstH2I RGCGCY 1 cut(s) 366
BstHHI GCGC 2 cut(s) 221, 365
BstKTI GATC 1 cut(s) 759
BstMBI GATC 1 cut(s) 756
BstMWI GCNNNNNNNGC 2 cut(s) 360, 784
BstSCI CCNGG 7 cut(s) 225, 398, 517, 518, 644, 744, 766
BstSLI GKGCMC 3 cut(s) 356, 422, 789
BstSNI TACGTA 1 cut(s) 329
BstUI CGCG 2 cut(s) 611, 803
BstV1I GCAGC 1 cut(s) 892
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 756
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 1 cut(s) 756
Bsu36I CCTNAGG 1 cut(s) 88
BsuI GTATCC 1 cut(s) 610
BsuRI GGCC 3 cut(s) 173, 354, 517
BtgI CCRYGG 1 cut(s) 801
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 4 cut(s) 63, 434, 518, 709
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 1 cut(s) 214
Cac8I GCNNGC 1 cut(s) 467
CfoI GCGC 2 cut(s) 221, 365
Cfr13I GGNCC 5 cut(s) 229, 352, 353, 402, 516
Cfr42I CCGCGG 1 cut(s) 804
Cfr9I CCCGGG 1 cut(s) 518
Csp6I GTAC 4 cut(s) 330, 577, 632, 653
CviAII CATG 2 cut(s) 448, 586
CviQI GTAC 4 cut(s) 330, 577, 632, 653
DdeI CTNAG 1 cut(s) 88
DinI GGCGCC 1 cut(s) 364
DpnI GATC 1 cut(s) 758
DpnII GATC 1 cut(s) 756
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 3 cut(s) 80, 106, 148
EarI CTCTTC 3 cut(s) 80, 106, 148
EciI GGCGGA 2 cut(s) 236, 592
Eco105I TACGTA 1 cut(s) 329
Eco130I CCWWGG 2 cut(s) 51, 342
Eco24I GRGCYC 3 cut(s) 356, 477, 861
Eco32I GATATC 1 cut(s) 739
Eco47I GGWCC 2 cut(s) 229, 402
Eco57I CTGAAG 1 cut(s) 132
Eco81I CCTNAGG 1 cut(s) 88
Eco88I CYCGRG 3 cut(s) 470, 518, 898
EcoRV GATATC 1 cut(s) 739
EcoT14I CCWWGG 2 cut(s) 51, 342
EcoT38I GRGCYC 3 cut(s) 356, 477, 861
EgeI GGCGCC 1 cut(s) 364
EheI GGCGCC 1 cut(s) 364
ErhI CCWWGG 2 cut(s) 51, 342
FaeI CATG 2 cut(s) 451, 589
FaiI YATR 7 cut(s) 449, 493, 545, 587, 626, 714, 855
FaqI GGGAC 1 cut(s) 215
FatI CATG 2 cut(s) 447, 585
FauI CCCGC 2 cut(s) 470, 700
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 2 cut(s) 804, 881
FokI GGATG 4 cut(s) 50, 421, 525, 716
FriOI GRGCYC 3 cut(s) 356, 477, 861
Fsp4HI GCNGC 2 cut(s) 804, 881
FspBI CTAG 3 cut(s) 41, 266, 846
GlaI GCGC 2 cut(s) 220, 364
GluI GCNGC 2 cut(s) 804, 881
HaeII RGCGCY 1 cut(s) 366
HaeIII GGCC 3 cut(s) 173, 354, 517
HapII CCGG 6 cut(s) 227, 400, 519, 645, 745, 768
HhaI GCGC 2 cut(s) 221, 365
Hin1I GRCGYC 2 cut(s) 125, 363
Hin1II CATG 2 cut(s) 451, 589
Hin6I GCGC 2 cut(s) 219, 363
HinP1I GCGC 2 cut(s) 219, 363
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HindIII AAGCTT 1 cut(s) 662
HinfI GANTC 2 cut(s) 204, 617
HpaII CCGG 6 cut(s) 227, 400, 519, 645, 745, 768
HphI GGTGA 6 cut(s) 70, 120, 126, 283, 698, 790
Hpy166II GTNNAC 1 cut(s) 454
Hpy188I TCNGA 3 cut(s) 203, 736, 756
Hpy188III TCNNGA 1 cut(s) 260
Hpy8I GTNNAC 1 cut(s) 454
Hpy99I CGWCG 3 cut(s) 127, 566, 616
HpyAV CCTTC 3 cut(s) 243, 280, 819
HpyCH4III ACNGT 2 cut(s) 534, 636
HpyCH4IV ACGT 5 cut(s) 125, 312, 328, 422, 564
HpyCH4V TGCA 1 cut(s) 589
HpyF10VI GCNNNNNNNGC 2 cut(s) 360, 784
HpyF3I CTNAG 1 cut(s) 88
HpySE526I ACGT 5 cut(s) 125, 312, 328, 422, 564
Hsp92I GRCGYC 2 cut(s) 125, 363
Hsp92II CATG 2 cut(s) 451, 589
HspAI GCGC 2 cut(s) 219, 363
KasI GGCGCC 1 cut(s) 362
KspI CCGCGG 1 cut(s) 804
Kzo9I GATC 1 cut(s) 756
LmnI GCTCC 2 cut(s) 431, 480
LpnPI CCDG 8 cut(s) 26, 155, 240, 413, 532, 658, 758, 781
Lsp1109I GCAGC 1 cut(s) 892
LweI GCATC 3 cut(s) 443, 540, 694
MaeI CTAG 3 cut(s) 41, 266, 846
MaeII ACGT 5 cut(s) 125, 312, 328, 422, 564
MaeIII GTNAC 3 cut(s) 126, 205, 832
MalI GATC 1 cut(s) 758
MboI GATC 1 cut(s) 756
MboII GAAGA 7 cut(s) 20, 28, 67, 93, 135, 138, 155
MflI RGATCY 1 cut(s) 756
MhlI GDGCHC 6 cut(s) 356, 378, 422, 477, 789, 861
MlsI TGGCCA 1 cut(s) 173
MluCI AATT 4 cut(s) 581, 721, 790, 864
MluNI TGGCCA 1 cut(s) 173
Mly113I GGCGCC 1 cut(s) 363
MlyI GAGTC 2 cut(s) 213, 626
MmeI TCCRAC 1 cut(s) 714
Mox20I TGGCCA 1 cut(s) 173
MscI TGGCCA 1 cut(s) 173
MseI TTAA 2 cut(s) 720, 867
MslI CAYNNNNRTG 2 cut(s) 413, 548
Msp20I TGGCCA 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 803
MspI CCGG 6 cut(s) 227, 400, 519, 645, 745, 768
MspR9I CCNGG 7 cut(s) 227, 400, 519, 520, 646, 746, 768
MvnI CGCG 2 cut(s) 611, 803
MwoI GCNNNNNNNGC 2 cut(s) 360, 784
NarI GGCGCC 1 cut(s) 363
NciI CCSGG 7 cut(s) 227, 400, 519, 520, 646, 746, 768
NdeII GATC 1 cut(s) 756
NlaIII CATG 2 cut(s) 451, 589
NlaIV GGNNCC 5 cut(s) 231, 354, 364, 476, 758
NmuCI GTSAC 2 cut(s) 126, 205
PcsI WCGNNNNNNNCGW 1 cut(s) 334
PflFI GACNNNGTC 2 cut(s) 227, 616
PkrI GCNGC 2 cut(s) 805, 882
PleI GAGTC 2 cut(s) 212, 625
PluTI GGCGCC 1 cut(s) 366
PpsI GAGTC 2 cut(s) 212, 625
Ppu21I YACGTR 1 cut(s) 329
PspN4I GGNNCC 5 cut(s) 231, 354, 364, 476, 758
PspOMI GGGCCC 1 cut(s) 352
PspPI GGNCC 5 cut(s) 229, 352, 353, 402, 516
PsuI RGATCY 1 cut(s) 756
PsyI GACNNNGTC 2 cut(s) 227, 616
RsaI GTAC 4 cut(s) 331, 578, 633, 654
RsaNI GTAC 4 cut(s) 330, 577, 632, 653
RseI CAYNNNNRTG 2 cut(s) 413, 548
SacII CCGCGG 1 cut(s) 804
SalI GTCGAC 1 cut(s) 452
SaqAI TTAA 2 cut(s) 720, 867
SatI GCNGC 2 cut(s) 804, 881
Sau3AI GATC 1 cut(s) 756
Sau96I GGNCC 5 cut(s) 229, 352, 353, 402, 516
SchI GAGTC 2 cut(s) 213, 626
ScrFI CCNGG 7 cut(s) 227, 400, 519, 520, 646, 746, 768
SduI GDGCHC 6 cut(s) 356, 378, 422, 477, 789, 861
SfaNI GCATC 3 cut(s) 443, 540, 694
SfoI GGCGCC 1 cut(s) 364
Sfr303I CCGCGG 1 cut(s) 804
SgrBI CCGCGG 1 cut(s) 804
SinI GGWCC 2 cut(s) 229, 402
SmaI CCCGGG 1 cut(s) 520
SmiMI CAYNNNNRTG 2 cut(s) 413, 548
SnaBI TACGTA 1 cut(s) 329
Sse9I AATT 4 cut(s) 581, 721, 790, 864
SsiI CCGC 8 cut(s) 247, 438, 463, 603, 707, 801, 803, 830
SspDI GGCGCC 1 cut(s) 362
SspMI CTAG 3 cut(s) 41, 266, 846
StyD4I CCNGG 7 cut(s) 225, 398, 517, 518, 644, 744, 766
StyI CCWWGG 2 cut(s) 51, 342
TaaI ACNGT 2 cut(s) 534, 636
TaiI ACGT 5 cut(s) 128, 315, 331, 425, 567
TaqI TCGA 7 cut(s) 192, 261, 372, 384, 411, 453, 498
TasI AATT 4 cut(s) 581, 721, 790, 864
TauI GCSGC 1 cut(s) 806
Tru1I TTAA 2 cut(s) 720, 867
Tru9I TTAA 2 cut(s) 720, 867
TscAI CASTG 1 cut(s) 221
TseFI GTSAC 2 cut(s) 126, 205
TseI GCWGC 1 cut(s) 880
Tsp45I GTSAC 2 cut(s) 126, 205
TspDTI ATGAA 2 cut(s) 17, 574
TspGWI ACGGA 1 cut(s) 629
TspMI CCCGGG 1 cut(s) 518
TspRI CASTG 1 cut(s) 221
Tth111I GACNNNGTC 2 cut(s) 227, 616
VpaK11BI GGWCC 2 cut(s) 229, 402
XcmI CCANNNNNNNNNTGG 1 cut(s) 412
XmaI CCCGGG 1 cut(s) 518
XmiI GTMKAC 1 cut(s) 453
XspI CTAG 3 cut(s) 41, 266, 846
ZraI GACGTC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.