Rroxscaffold_4G00295780

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016587
Physical Location & Seq
Forward (+)
15822264 .. 15824957
2694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00295780.1

Sequence Viewer

Length: 966 bp
ATGCAGTGGATCCTGGAATCAGATGAAACAAGCAGTGACAGACAACAGATTAGTCCCAAGAGTGTTAGAGGTGGGAAAAATGCGGTGTTCTGTACTTCATGTCTGTCAATTTGTGGCTTACAATATGGCAATAAGCATGCAGCATACTCCGTGAATTCTGATCCTGCTACAAGTTTTGTTGACGTCACCCTCACCACCCACTTCTTCACCCCCTCGCTTCTTCAACTGGCCAACCTCCCCACCCAACTCGACCACTTCCGAGTCACAACTCACTGCGCCGACCCGGTCCCTTCCCAACTCGTCCGCCAAACCATTCTCGACCTAGTCTTCAACTCCACCTCGCCTTTCACCAACTTCCCTCCTCCCCACGTCAAAAGCCTCCTACGTACCAATCGCGCTAAGGGATTGGGCCCAAACGGCGCCGTTTTCGAGCACTTAATCGAAATGGTCAAGCCCCGGACCATCATCGAAGTGGGCACGTTTCTTGGAGCATCCGCTATCCACATGGTCGACCTGACCCGCAAGCTCGGGCTCCAAACCCAAATCCTATGTGTCGATGACTTTCGGGGCTGGCCCGGGTTTAGAGAACAGTTCAAGGACATAAAGATGCTAAACGGCGACGTTTTTTTGATGTACCAATTCATGCAAAATATCGTATCCGCCAACGCAACAGAGTCGGTTTTACCCGTACCGTTTTCAACCGGGTCGGTACTTGATAAGCTTTGTGAGTGGGGTATTTTGGGTGATTTGATAGAAGTGGATGCGGGTTATGATTTTAATTCGGCTTGGTCGGATATCAACCGGGCTTACCGGATCCTAAAACCCGGTGGGGTGCTTTTCGGGCACAATTATTTCACCGCGGCTGATGATAGAGGAGTAAGAAGGGCGGTAACTTTATTTGCTCGGGTTTATGGGCTCAAAATTAAACTTGAAAGGCAGCATTGGGTCATTCACTCGGGTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_24

Protein Analysis

321

Amino Acids

35.84

Weight (kDa)

7.68

Isoelectric Point (pI)

30.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_24 PF13578 156 - 284 1.2e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016587)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01180
malus_domestica MD07G1156900.v1.1
prunus_persica Prupe.2G195400_v2.0.a1
pyrus_communis pycom07g15350
rosa_chinensis RchiOBHm_Chr1g0360821 RchiOBHm_Chr1g0360831
rosa_laevigata RLG00000027783
rosa_multiflora Rmu_ssc0000022.1_g000046
rosa_roxburghii Rroxscaffold_4G00295780
rosa_rugosa Rorug01G0281900
rosa_samantha Rh1AG293800 Rh1BG258600 Rh1DG288800
rosa_wichuraiana Rw1G026000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 419
AccI GTMKAC 1 cut(s) 510
AccII CGCG 2 cut(s) 396, 860
AciI CCGC 9 cut(s) 83, 304, 495, 520, 660, 764, 858, 860, 887
AclWI GGATC 5 cut(s) 4, 17, 155, 808, 821
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 154
AcyI GRCGYC 2 cut(s) 183, 420
AfaI GTAC 5 cut(s) 94, 388, 635, 690, 711
AgsI TTSAA 5 cut(s) 224, 331, 595, 699, 932
AjiI CACGTC 1 cut(s) 370
AjnI CCWGG 1 cut(s) 12
AluBI AGCT 2 cut(s) 526, 721
AluI AGCT 2 cut(s) 526, 721
Alw21I GWGCWC 1 cut(s) 435
AlwI GGATC 5 cut(s) 4, 17, 155, 808, 821
Ama87I CYCGRG 4 cut(s) 527, 575, 903, 955
AoxI GGCC 3 cut(s) 228, 409, 572
ApaI GGGCCC 1 cut(s) 413
ApeKI GCWGC 2 cut(s) 140, 937
ApoI RAATTY 1 cut(s) 154
AspLEI GCGC 3 cut(s) 278, 398, 422
AspS9I GGNCC 5 cut(s) 286, 409, 410, 459, 573
AsuC2I CCSGG 7 cut(s) 284, 457, 576, 577, 703, 803, 825
AsuHPI GGTGA 6 cut(s) 178, 184, 199, 340, 755, 847
AvaI CYCGRG 4 cut(s) 527, 575, 903, 955
AvaII GGWCC 2 cut(s) 286, 459
BaeGI GKGCMC 3 cut(s) 413, 479, 846
BalI TGGCCA 1 cut(s) 230
BamHI GGATCC 2 cut(s) 9, 813
BanI GGYRCC 1 cut(s) 419
BanII GRGCYC 3 cut(s) 413, 534, 918
BbsI GAAGAC 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 435
BbvI GCAGC 2 cut(s) 152, 949
BccI CCATC 1 cut(s) 470
BceAI ACGGC 3 cut(s) 407, 433, 631
BcgI CGANNNNNNTGC 2 cut(s) 657, 691
BciT130I CCWGG 1 cut(s) 14
BciVI GTATCC 1 cut(s) 667
BcnI CCSGG 7 cut(s) 284, 457, 576, 577, 703, 803, 825
BfaI CTAG 1 cut(s) 323
BfoI RGCGCY 1 cut(s) 423
BfuI GTATCC 1 cut(s) 667
BglI GCCNNNNNGGC 1 cut(s) 417
BisI GCNGC 3 cut(s) 141, 861, 938
BlsI GCNGC 3 cut(s) 142, 862, 939
Bme1390I CCNGG 8 cut(s) 14, 284, 457, 576, 577, 703, 803, 825
Bme18I GGWCC 2 cut(s) 286, 459
BmeT110I CYCGRG 4 cut(s) 527, 575, 903, 955
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 5 cut(s) 286, 409, 410, 459, 573
BmiI GGNNCC 6 cut(s) 11, 288, 411, 421, 533, 815
BmrFI CCNGG 8 cut(s) 14, 284, 457, 576, 577, 703, 803, 825
BmsI GCATC 3 cut(s) 500, 597, 751
BpiI GAAGAC 1 cut(s) 319
Bpu10I CCTNAGC 1 cut(s) 399
BpuMI CCSGG 7 cut(s) 284, 457, 576, 577, 703, 803, 825
BsaAI YACGTR 1 cut(s) 386
BsaHI GRCGYC 2 cut(s) 183, 420
BsaJI CCNNGG 3 cut(s) 455, 575, 858
BsaWI WCCGGW 1 cut(s) 810
Bse1I ACTGG 1 cut(s) 231
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 3 cut(s) 455, 575, 858
BseGI GGATG 2 cut(s) 491, 766
BseNI ACTGG 1 cut(s) 231
BseRI GAGGAG 2 cut(s) 351, 888
BseSI GKGCMC 3 cut(s) 413, 479, 846
BseXI GCAGC 2 cut(s) 152, 949
Bsh1236I CGCG 2 cut(s) 396, 860
BshFI GGCC 3 cut(s) 230, 411, 574
BshNI GGYRCC 1 cut(s) 419
BsiHKAI GWGCWC 1 cut(s) 435
BsiHKCI CYCGRG 4 cut(s) 527, 575, 903, 955
BsiSI CCGG 7 cut(s) 284, 457, 576, 702, 802, 811, 825
BslFI GGGAC 2 cut(s) 39, 272
BsmFI GGGAC 2 cut(s) 39, 272
BsnI GGCC 3 cut(s) 230, 411, 574
BsoBI CYCGRG 4 cut(s) 527, 575, 903, 955
Bsp120I GGGCCC 1 cut(s) 409
Bsp1286I GDGCHC 6 cut(s) 413, 435, 479, 534, 846, 918
Bsp143I GATC 3 cut(s) 9, 160, 813
BspACI CCGC 9 cut(s) 83, 304, 495, 520, 660, 764, 858, 860, 887
BspANI GGCC 3 cut(s) 230, 411, 574
BspFNI CGCG 2 cut(s) 396, 860
BspLI GGNNCC 6 cut(s) 11, 288, 411, 421, 533, 815
BspPI GGATC 5 cut(s) 4, 17, 155, 808, 821
BspT107I GGYRCC 1 cut(s) 419
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 3 cut(s) 455, 575, 858
BssMI GATC 3 cut(s) 9, 160, 813
BssNI GRCGYC 2 cut(s) 183, 420
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 2 cut(s) 591, 693
BstACI GRCGYC 2 cut(s) 183, 420
BstBAI YACGTR 1 cut(s) 386
BstC8I GCNNGC 3 cut(s) 138, 524, 572
BstDEI CTNAG 1 cut(s) 399
BstDSI CCRYGG 1 cut(s) 858
BstF5I GGATG 2 cut(s) 491, 766
BstFNI CGCG 2 cut(s) 396, 860
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 3 cut(s) 278, 398, 422
BstKTI GATC 3 cut(s) 12, 163, 816
BstMBI GATC 3 cut(s) 9, 160, 813
BstMWI GCNNNNNNNGC 2 cut(s) 417, 841
BstNI CCWGG 1 cut(s) 14
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 8 cut(s) 12, 282, 455, 574, 575, 701, 801, 823
BstSLI GKGCMC 3 cut(s) 413, 479, 846
BstSNI TACGTA 1 cut(s) 386
BstUI CGCG 2 cut(s) 396, 860
BstV1I GCAGC 2 cut(s) 152, 949
BstV2I GAAGAC 1 cut(s) 319
BstX2I RGATCY 2 cut(s) 9, 813
BstYI RGATCY 2 cut(s) 9, 813
BsuI GTATCC 1 cut(s) 667
BsuRI GGCC 3 cut(s) 230, 411, 574
BtgI CCRYGG 1 cut(s) 858
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 2 cut(s) 491, 766
BtsI GCAGTG 3 cut(s) 11, 40, 271
BtsIMutI CAGTG 3 cut(s) 11, 40, 271
Cac8I GCNNGC 3 cut(s) 138, 524, 572
CfoI GCGC 3 cut(s) 278, 398, 422
Cfr13I GGNCC 5 cut(s) 286, 409, 410, 459, 573
Cfr42I CCGCGG 1 cut(s) 861
Cfr9I CCCGGG 1 cut(s) 575
Csp6I GTAC 5 cut(s) 93, 387, 634, 689, 710
CviAII CATG 4 cut(s) 99, 137, 505, 643
CviQI GTAC 5 cut(s) 93, 387, 634, 689, 710
DdeI CTNAG 1 cut(s) 399
DinI GGCGCC 1 cut(s) 421
DpnI GATC 3 cut(s) 11, 162, 815
DpnII GATC 3 cut(s) 9, 160, 813
EaeI YGGCCR 1 cut(s) 228
EciI GGCGGA 2 cut(s) 293, 649
Eco105I TACGTA 1 cut(s) 386
Eco24I GRGCYC 3 cut(s) 413, 534, 918
Eco32I GATATC 1 cut(s) 796
Eco47I GGWCC 2 cut(s) 286, 459
Eco88I CYCGRG 4 cut(s) 527, 575, 903, 955
EcoRI GAATTC 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 12
EcoRV GATATC 1 cut(s) 796
EcoT38I GRGCYC 3 cut(s) 413, 534, 918
EgeI GGCGCC 1 cut(s) 421
EheI GGCGCC 1 cut(s) 421
FaeI CATG 4 cut(s) 102, 140, 508, 646
FaqI GGGAC 2 cut(s) 39, 272
FatI CATG 4 cut(s) 98, 136, 504, 642
FauI CCCGC 2 cut(s) 527, 757
FblI GTMKAC 1 cut(s) 510
Fnu4HI GCNGC 3 cut(s) 141, 861, 938
FokI GGATG 2 cut(s) 478, 773
FriOI GRGCYC 3 cut(s) 413, 534, 918
Fsp4HI GCNGC 3 cut(s) 141, 861, 938
FspBI CTAG 1 cut(s) 323
GlaI GCGC 3 cut(s) 277, 397, 421
GluI GCNGC 3 cut(s) 141, 861, 938
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 3 cut(s) 230, 411, 574
HapII CCGG 7 cut(s) 284, 457, 576, 702, 802, 811, 825
HhaI GCGC 3 cut(s) 278, 398, 422
Hin1I GRCGYC 2 cut(s) 183, 420
Hin1II CATG 4 cut(s) 102, 140, 508, 646
Hin6I GCGC 3 cut(s) 276, 396, 420
HinP1I GCGC 3 cut(s) 276, 396, 420
HincII GTYRAC 2 cut(s) 181, 511
HindII GTYRAC 2 cut(s) 181, 511
HindIII AAGCTT 1 cut(s) 719
HinfI GANTC 3 cut(s) 17, 261, 674
HpaII CCGG 7 cut(s) 284, 457, 576, 702, 802, 811, 825
HphI GGTGA 6 cut(s) 178, 184, 199, 340, 755, 847
Hpy166II GTNNAC 2 cut(s) 181, 511
Hpy188I TCNGA 4 cut(s) 22, 160, 260, 793
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 2 cut(s) 181, 511
Hpy99I CGWCG 1 cut(s) 623
HpyAV CCTTC 2 cut(s) 300, 876
HpyCH4III ACNGT 2 cut(s) 591, 693
HpyCH4IV ACGT 5 cut(s) 183, 369, 385, 479, 621
HpyCH4V TGCA 3 cut(s) 4, 140, 646
HpyF10VI GCNNNNNNNGC 2 cut(s) 417, 841
HpyF3I CTNAG 1 cut(s) 399
HpySE526I ACGT 5 cut(s) 183, 369, 385, 479, 621
Hsp92I GRCGYC 2 cut(s) 183, 420
Hsp92II CATG 4 cut(s) 102, 140, 508, 646
HspAI GCGC 3 cut(s) 276, 396, 420
KasI GGCGCC 1 cut(s) 419
KspI CCGCGG 1 cut(s) 861
Kzo9I GATC 3 cut(s) 9, 160, 813
LmnI GCTCC 2 cut(s) 488, 537
Lsp1109I GCAGC 2 cut(s) 152, 949
LweI GCATC 3 cut(s) 500, 597, 751
MaeI CTAG 1 cut(s) 323
MaeII ACGT 5 cut(s) 183, 369, 385, 479, 621
MaeIII GTNAC 4 cut(s) 35, 184, 262, 889
MalI GATC 3 cut(s) 11, 162, 815
MboI GATC 3 cut(s) 9, 160, 813
MboII GAAGA 3 cut(s) 196, 212, 319
MflI RGATCY 2 cut(s) 9, 813
MhlI GDGCHC 6 cut(s) 413, 435, 479, 534, 846, 918
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 6 cut(s) 108, 154, 638, 778, 847, 921
MluNI TGGCCA 1 cut(s) 230
Mly113I GGCGCC 1 cut(s) 420
MlyI GAGTC 2 cut(s) 270, 683
MmeI TCCRAC 1 cut(s) 771
MnlI CCTC 9 cut(s) 62, 200, 223, 245, 349, 369, 372, 389, 866
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
MseI TTAA 3 cut(s) 437, 777, 924
MslI CAYNNNNRTG 2 cut(s) 470, 605
Msp20I TGGCCA 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 860
MspI CCGG 7 cut(s) 284, 457, 576, 702, 802, 811, 825
MspR9I CCNGG 8 cut(s) 14, 284, 457, 576, 577, 703, 803, 825
MvaI CCWGG 1 cut(s) 14
MvnI CGCG 2 cut(s) 396, 860
MwoI GCNNNNNNNGC 2 cut(s) 417, 841
NarI GGCGCC 1 cut(s) 420
NciI CCSGG 7 cut(s) 284, 457, 576, 577, 703, 803, 825
NdeII GATC 3 cut(s) 9, 160, 813
NlaIII CATG 4 cut(s) 102, 140, 508, 646
NlaIV GGNNCC 6 cut(s) 11, 288, 411, 421, 533, 815
NmuCI GTSAC 3 cut(s) 35, 184, 262
NspI RCATGY 1 cut(s) 140
PaeI GCATGC 1 cut(s) 140
PfeI GAWTC 1 cut(s) 17
PflFI GACNNNGTC 2 cut(s) 284, 323
PfoI TCCNGGA 1 cut(s) 12
PkrI GCNGC 3 cut(s) 142, 862, 939
PleI GAGTC 2 cut(s) 269, 682
PluTI GGCGCC 1 cut(s) 423
PpsI GAGTC 2 cut(s) 269, 682
Ppu21I YACGTR 1 cut(s) 386
Psp6I CCWGG 1 cut(s) 12
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 6 cut(s) 11, 288, 411, 421, 533, 815
PspOMI GGGCCC 1 cut(s) 409
PspPI GGNCC 5 cut(s) 286, 409, 410, 459, 573
PsuI RGATCY 2 cut(s) 9, 813
PsyI GACNNNGTC 2 cut(s) 284, 323
RsaI GTAC 5 cut(s) 94, 388, 635, 690, 711
RsaNI GTAC 5 cut(s) 93, 387, 634, 689, 710
RseI CAYNNNNRTG 2 cut(s) 470, 605
SacII CCGCGG 1 cut(s) 861
SalI GTCGAC 1 cut(s) 509
SaqAI TTAA 3 cut(s) 437, 777, 924
SatI GCNGC 3 cut(s) 141, 861, 938
Sau3AI GATC 3 cut(s) 9, 160, 813
Sau96I GGNCC 5 cut(s) 286, 409, 410, 459, 573
SchI GAGTC 2 cut(s) 270, 683
ScrFI CCNGG 8 cut(s) 14, 284, 457, 576, 577, 703, 803, 825
SduI GDGCHC 6 cut(s) 413, 435, 479, 534, 846, 918
SfaNI GCATC 3 cut(s) 500, 597, 751
SfoI GGCGCC 1 cut(s) 421
Sfr303I CCGCGG 1 cut(s) 861
SgrBI CCGCGG 1 cut(s) 861
SinI GGWCC 2 cut(s) 286, 459
SmaI CCCGGG 1 cut(s) 577
SmiMI CAYNNNNRTG 2 cut(s) 470, 605
SnaBI TACGTA 1 cut(s) 386
SphI GCATGC 1 cut(s) 140
Sse9I AATT 6 cut(s) 108, 154, 638, 778, 847, 921
SsiI CCGC 9 cut(s) 83, 304, 495, 520, 660, 764, 858, 860, 887
SspDI GGCGCC 1 cut(s) 419
SspMI CTAG 1 cut(s) 323
StyD4I CCNGG 8 cut(s) 12, 282, 455, 574, 575, 701, 801, 823
TaaI ACNGT 2 cut(s) 591, 693
TaiI ACGT 5 cut(s) 186, 372, 388, 482, 624
TaqI TCGA 7 cut(s) 249, 318, 429, 441, 468, 510, 555
TasI AATT 6 cut(s) 108, 154, 638, 778, 847, 921
TatI WGTACW 1 cut(s) 92
TauI GCSGC 1 cut(s) 863
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 3 cut(s) 437, 777, 924
Tru9I TTAA 3 cut(s) 437, 777, 924
TscAI CASTG 2 cut(s) 40, 278
TseFI GTSAC 3 cut(s) 35, 184, 262
TseI GCWGC 2 cut(s) 140, 937
Tsp45I GTSAC 3 cut(s) 35, 184, 262
TspDTI ATGAA 3 cut(s) 39, 87, 631
TspGWI ACGGA 1 cut(s) 139
TspMI CCCGGG 1 cut(s) 575
TspRI CASTG 2 cut(s) 40, 278
Tth111I GACNNNGTC 2 cut(s) 284, 323
VpaK11BI GGWCC 2 cut(s) 286, 459
XapI RAATTY 1 cut(s) 154
XceI RCATGY 1 cut(s) 140
XcmI CCANNNNNNNNNTGG 1 cut(s) 469
XmaI CCCGGG 1 cut(s) 575
XmiI GTMKAC 1 cut(s) 510
XspI CTAG 1 cut(s) 323
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.