Rmu_ssc0000119.1_g000001

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000119.1
OG0007510
Physical Location & Seq
Reverse (-)
358 .. 3864
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1386 bp
atgaataggtctttgcttgatggtatagagcacctgagctgttatttggcaccaaacaatatggacctgggctggatgtgtgggctgcagcttagagagaaagctcagagcaaaacccaaaaccaatcaatcaatcatggtgtcctcctctccaataacaaaaccctccattcccttcttgcctcaaacctcaccaagctcacaatcccaccttcctcctcctcctcctcttctccgcctcaagacttcgactttaacgacgtcttcggccccccaaccgcctccaacgacggcggcgcgtccacttcattctcgccgtcgttactcgtcatccacaaccggtcccactccttcgtcggcccctccccgcgcttcactccctcctccctcctccccttcaccgccgccgactccgactccgaggaagacgaaaacgacgccgtttccgaaaccgtgaaagatgaggaatcggcggttcggaagttcgggccttgggatttcgagattctcagagtcgtcggcaagggcgcgttcgggaaggtgtttcaggtgaggaagaagagcagtgatggcgatgaagatggtgatgggctttatgccatgaaggtcatgaggaaggagactgttatcaagaagaaccatgtggattacatgaaggctgagagggacattctcaccaaagttgtgcacccttttattgttcagctccgctactcttttcagaccaagtgtaagctgtacttgatcatggatttcatgaatggagggcatttgttctatcatttgtaccggcaggggatattcagtgaggatcaggcgaggttttacactgctgagatagtgtctgctgtttcacatcttcacaaatgcgggattgtgcatcgggaccttaagccggaaaatgttctcatggatgctgatgggcatgtgatgctgacggattttggactagcaaaggaaattgatgaagatagcagatcgaattcaatgtgtggaaccaccgaatacatggctcctgaaattctgctgtctaaaggccacaacaaagatgcagattggtggagcattggtatacttttgtatgagatgctaagtggtcagttgctacaaaaggaaccatcccaaaggttaggcagtggccccagtggaggtgataatatcaaaagtcataaatggtttaaaacaatcaactggaagaagttggaggccagagaactgctcccaaagttcaagccagatgtgactggaaaagaatgcacggccaattttgaccggtgctggacaacaatgcctctagatgactcaccagctcctacaccaacagcgggtgagcattttcagggatatacttacgtggcacctaacacttggctttcatctgtataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

461

Amino Acids

51.5

Weight (kDa)

5.98

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 167 - 371 9e-64 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 168 - 366 1.6e-34 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 247 - 328 2.8e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 271 - 368 2.5e-07 Kinase-like
Pkinase_fungal PF17667 280 - 352 2.2e-08 Fungal protein kinase
Pkinase_C PF00433 414 - 451 1.8e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 264
AccB1I GGYRCC 2 cut(s) 49, 1357
AccI GTMKAC 1 cut(s) 1072
AccII CGCG 3 cut(s) 299, 370, 530
AclWI GGATC 1 cut(s) 819
AcoI YGGCCR 1 cut(s) 1260
AcsI RAATTY 2 cut(s) 982, 1020
AcyI GRCGYC 2 cut(s) 261, 438
AfaI GTAC 2 cut(s) 740, 788
AfiI CCNNNNNNNGG 3 cut(s) 895, 1148, 1325
AflII CTTAAG 1 cut(s) 890
AgeI ACCGGT 2 cut(s) 339, 1272
AgsI TTSAA 2 cut(s) 987, 1231
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 7 cut(s) 39, 91, 104, 199, 706, 736, 1310
AluI AGCT 7 cut(s) 39, 91, 104, 199, 706, 736, 1310
Alw21I GWGCWC 2 cut(s) 33, 690
Alw26I GTCTC 1 cut(s) 614
Alw44I GTGCAC 1 cut(s) 686
AlwI GGATC 1 cut(s) 819
AoxI GGCC 7 cut(s) 268, 358, 488, 1036, 1138, 1206, 1260
ApaLI GTGCAC 1 cut(s) 686
ApeKI GCWGC 2 cut(s) 85, 88
ApoI RAATTY 2 cut(s) 982, 1020
AsiGI ACCGGT 2 cut(s) 339, 1272
Asp700I GAANNNNTTC 1 cut(s) 903
AspLEI GCGC 3 cut(s) 299, 372, 530
AspS9I GGNCC 7 cut(s) 64, 269, 342, 359, 488, 886, 1139
AsuHPI GGTGA 8 cut(s) 184, 391, 562, 596, 667, 1163, 1296, 1340
AvaII GGWCC 3 cut(s) 64, 342, 886
BaeGI GKGCMC 1 cut(s) 690
BanI GGYRCC 2 cut(s) 49, 1357
BbsI GAAGAC 2 cut(s) 256, 432
Bbv12I GWGCWC 2 cut(s) 33, 690
BbvI GCAGC 2 cut(s) 72, 100
BccI CCATC 6 cut(s) 14, 563, 575, 581, 914, 1126
BceAI ACGGC 4 cut(s) 301, 307, 425, 1275
BciT130I CCWGG 1 cut(s) 68
BclI TGATCA 1 cut(s) 744
BcoDI GTCTC 1 cut(s) 614
BfaI CTAG 2 cut(s) 950, 1295
BfmI CTRYAG 1 cut(s) 86
BfrI CTTAAG 1 cut(s) 890
BisI GCNGC 4 cut(s) 86, 89, 295, 405
BlsI GCNGC 4 cut(s) 87, 90, 296, 406
Bme1390I CCNGG 1 cut(s) 68
Bme18I GGWCC 3 cut(s) 64, 342, 886
BmgT120I GGNCC 7 cut(s) 64, 269, 342, 359, 488, 886, 1139
BmrFI CCNGG 1 cut(s) 68
BmrI ACTGGG 1 cut(s) 1137
BmsI GCATC 5 cut(s) 889, 904, 921, 1039, 1077
BmuI ACTGGG 1 cut(s) 1137
BpiI GAAGAC 2 cut(s) 256, 432
BplI GAGNNNNNCTC 2 cut(s) 1203, 1235
Bpu10I CCTNAGC 1 cut(s) 35
BpuEI CTTGAG 1 cut(s) 225
BsaAI YACGTR 1 cut(s) 1354
BsaHI GRCGYC 2 cut(s) 261, 438
BsaJI CCNNGG 3 cut(s) 67, 420, 491
BsaWI WCCGGW 2 cut(s) 339, 1272
BsaXI ACNNNNNCTCC 4 cut(s) 401, 431, 1054, 1084
Bsc4I CCNNNNNNNGG 3 cut(s) 895, 1148, 1325
Bse118I RCCGGY 3 cut(s) 339, 789, 1272
Bse1I ACTGG 3 cut(s) 1143, 1196, 1249
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 3 cut(s) 67, 420, 491
BseGI GGATG 4 cut(s) 81, 330, 919, 1118
BseLI CCNNNNNNNGG 3 cut(s) 895, 1148, 1325
BseMII CTCAG 5 cut(s) 26, 119, 523, 651, 825
BseNI ACTGG 3 cut(s) 1143, 1196, 1249
BseRI GAGGAG 7 cut(s) 137, 208, 211, 214, 217, 373, 380
BseSI GKGCMC 1 cut(s) 690
BseXI GCAGC 2 cut(s) 72, 100
Bsh1236I CGCG 3 cut(s) 299, 370, 530
BshFI GGCC 7 cut(s) 270, 360, 490, 1038, 1140, 1208, 1262
BshNI GGYRCC 2 cut(s) 49, 1357
BshTI ACCGGT 2 cut(s) 339, 1272
BsiHKAI GWGCWC 2 cut(s) 33, 690
BsiSI CCGG 4 cut(s) 340, 790, 896, 1273
BslFI GGGAC 3 cut(s) 328, 680, 899
BslI CCNNNNNNNGG 3 cut(s) 895, 1148, 1325
BsmAI GTCTC 1 cut(s) 614
BsmFI GGGAC 3 cut(s) 328, 680, 899
BsmI GAATGC 1 cut(s) 1259
BsnI GGCC 7 cut(s) 270, 360, 490, 1038, 1140, 1208, 1262
Bsp1286I GDGCHC 2 cut(s) 33, 690
Bsp143I GATC 3 cut(s) 744, 811, 977
BspANI GGCC 7 cut(s) 270, 360, 490, 1038, 1140, 1208, 1262
BspCNI CTCAG 5 cut(s) 27, 118, 522, 652, 826
BspFNI CGCG 3 cut(s) 299, 370, 530
BspHI TCATGA 2 cut(s) 609, 756
BspMAI CTGCAG 1 cut(s) 90
BspPI GGATC 1 cut(s) 819
BspQI GCTCTTC 1 cut(s) 554
BspT107I GGYRCC 2 cut(s) 49, 1357
BspTI CTTAAG 1 cut(s) 890
BsrFI RCCGGY 3 cut(s) 339, 789, 1272
BsrI ACTGG 3 cut(s) 1143, 1196, 1249
BssAI RCCGGY 3 cut(s) 339, 789, 1272
BssECI CCNNGG 3 cut(s) 67, 420, 491
BssMI GATC 3 cut(s) 744, 811, 977
BssNAI GTATAC 1 cut(s) 1073
BssNI GRCGYC 2 cut(s) 261, 438
BssT1I CCWWGG 1 cut(s) 491
Bst1107I GTATAC 1 cut(s) 1073
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 2 cut(s) 454, 625
Bst6I CTCTTC 2 cut(s) 235, 554
BstACI GRCGYC 2 cut(s) 261, 438
BstAFI CTTAAG 1 cut(s) 890
BstAPI GCANNNNNTGC 1 cut(s) 931
BstBAI YACGTR 1 cut(s) 1354
BstDEI CTNAG 7 cut(s) 35, 92, 105, 509, 660, 834, 1091
BstF5I GGATG 4 cut(s) 81, 330, 919, 1118
BstFNI CGCG 3 cut(s) 299, 370, 530
BstHHI GCGC 3 cut(s) 299, 372, 530
BstKTI GATC 3 cut(s) 747, 814, 980
BstMAI GTCTC 1 cut(s) 614
BstMBI GATC 3 cut(s) 744, 811, 977
BstMWI GCNNNNNNNGC 2 cut(s) 570, 931
BstNI CCWGG 1 cut(s) 68
BstNSI RCATGY 1 cut(s) 929
BstSCI CCNGG 1 cut(s) 66
BstSFI CTRYAG 1 cut(s) 86
BstSLI GKGCMC 1 cut(s) 690
BstUI CGCG 3 cut(s) 299, 370, 530
BstV1I GCAGC 2 cut(s) 72, 100
BstV2I GAAGAC 2 cut(s) 256, 432
BstZ17I GTATAC 1 cut(s) 1073
BsuRI GGCC 7 cut(s) 270, 360, 490, 1038, 1140, 1208, 1262
BtgZI GCGATG 1 cut(s) 588
BtsCI GGATG 4 cut(s) 81, 330, 919, 1118
BtsI GCAGTG 3 cut(s) 571, 828, 1141
BtsIMutI CAGTG 5 cut(s) 571, 811, 828, 1141, 1150
CciI TCATGA 2 cut(s) 609, 756
CfoI GCGC 3 cut(s) 299, 372, 530
Cfr10I RCCGGY 3 cut(s) 339, 789, 1272
Cfr13I GGNCC 7 cut(s) 64, 269, 342, 359, 488, 886, 1139
CseI GACGC 2 cut(s) 288, 446
Csp6I GTAC 2 cut(s) 739, 787
CspAI ACCGGT 2 cut(s) 339, 1272
CviQI GTAC 2 cut(s) 739, 787
DdeI CTNAG 7 cut(s) 35, 92, 105, 509, 660, 834, 1091
DpnI GATC 3 cut(s) 746, 813, 979
DpnII GATC 3 cut(s) 744, 811, 977
DraI TTTAAA 1 cut(s) 1180
EaeI YGGCCR 1 cut(s) 1260
Eam1104I CTCTTC 2 cut(s) 235, 554
EarI CTCTTC 2 cut(s) 235, 554
EciI GGCGGA 1 cut(s) 225
Eco130I CCWWGG 1 cut(s) 491
Eco47I GGWCC 3 cut(s) 64, 342, 886
EcoO109I RGGNCCY 1 cut(s) 886
EcoRI GAATTC 1 cut(s) 982
EcoRII CCWGG 1 cut(s) 66
EcoT14I CCWWGG 1 cut(s) 491
ErhI CCWWGG 1 cut(s) 491
FalI AAGNNNNNCTT 2 cut(s) 725, 757
FaqI GGGAC 3 cut(s) 328, 680, 899
FauI CCCGC 3 cut(s) 375, 863, 1318
FbaI TGATCA 1 cut(s) 744
FblI GTMKAC 1 cut(s) 1072
Fnu4HI GCNGC 4 cut(s) 86, 89, 295, 405
FokI GGATG 4 cut(s) 88, 317, 926, 1105
Fsp4HI GCNGC 4 cut(s) 86, 89, 295, 405
FspBI CTAG 2 cut(s) 950, 1295
GlaI GCGC 3 cut(s) 298, 371, 529
GluI GCNGC 4 cut(s) 86, 89, 295, 405
HaeIII GGCC 7 cut(s) 270, 360, 490, 1038, 1140, 1208, 1262
HapII CCGG 4 cut(s) 340, 790, 896, 1273
HgaI GACGC 2 cut(s) 288, 446
HhaI GCGC 3 cut(s) 299, 372, 530
Hin1I GRCGYC 2 cut(s) 261, 438
Hin6I GCGC 3 cut(s) 297, 370, 528
HinP1I GCGC 3 cut(s) 297, 370, 528
HinfI GANTC 6 cut(s) 410, 416, 467, 505, 513, 1301
HpaII CCGG 4 cut(s) 340, 790, 896, 1273
HphI GGTGA 8 cut(s) 184, 391, 562, 596, 667, 1163, 1296, 1340
Hpy166II GTNNAC 3 cut(s) 303, 688, 1073
Hpy188I TCNGA 7 cut(s) 108, 415, 421, 448, 480, 512, 723
Hpy188III TCNNGA 9 cut(s) 242, 502, 535, 610, 631, 757, 884, 1016, 1295
Hpy8I GTNNAC 3 cut(s) 303, 688, 1073
Hpy99I CGWCG 6 cut(s) 263, 293, 322, 359, 440, 521
HpyAV CCTTC 8 cut(s) 185, 222, 361, 406, 532, 598, 610, 649
HpyCH4III ACNGT 2 cut(s) 454, 625
HpyCH4IV ACGT 2 cut(s) 261, 1353
HpyCH4V TGCA 5 cut(s) 88, 688, 880, 1052, 1257
HpyF10VI GCNNNNNNNGC 2 cut(s) 570, 931
HpyF3I CTNAG 7 cut(s) 35, 92, 105, 509, 660, 834, 1091
HpySE526I ACGT 2 cut(s) 261, 1353
Hsp92I GRCGYC 2 cut(s) 261, 438
HspAI GCGC 3 cut(s) 297, 370, 528
Ksp22I TGATCA 1 cut(s) 744
Kzo9I GATC 3 cut(s) 744, 811, 977
LguI GCTCTTC 1 cut(s) 554
LmnI GCTCC 5 cut(s) 711, 1018, 1062, 1224, 1315
Lsp1109I GCAGC 2 cut(s) 72, 100
LweI GCATC 5 cut(s) 889, 904, 921, 1039, 1077
MaeI CTAG 2 cut(s) 950, 1295
MaeII ACGT 2 cut(s) 261, 1353
MaeIII GTNAC 2 cut(s) 321, 1240
MalI GATC 3 cut(s) 746, 813, 979
MboI GATC 3 cut(s) 744, 811, 977
MhlI GDGCHC 2 cut(s) 33, 690
MluCI AATT 4 cut(s) 960, 982, 1020, 1264
MlyI GAGTC 4 cut(s) 404, 410, 522, 1295
MmeI TCCRAC 3 cut(s) 309, 438, 1182
MroXI GAANNNNTTC 1 cut(s) 903
MseI TTAA 3 cut(s) 255, 891, 1179
MspA1I CMGCKG 1 cut(s) 1325
MspCI CTTAAG 1 cut(s) 890
MspI CCGG 4 cut(s) 340, 790, 896, 1273
MspR9I CCNGG 1 cut(s) 68
Mva1269I GAATGC 1 cut(s) 1259
MvaI CCWGG 1 cut(s) 68
MvnI CGCG 3 cut(s) 299, 370, 530
MwoI GCNNNNNNNGC 2 cut(s) 570, 931
NdeII GATC 3 cut(s) 744, 811, 977
NmuCI GTSAC 1 cut(s) 1240
NspI RCATGY 1 cut(s) 929
PagI TCATGA 2 cut(s) 609, 756
PciSI GCTCTTC 1 cut(s) 554
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 444
PctI GAATGC 1 cut(s) 1259
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 2 cut(s) 467, 505
PinAI ACCGGT 2 cut(s) 339, 1272
PkrI GCNGC 4 cut(s) 87, 90, 296, 406
PleI GAGTC 4 cut(s) 404, 410, 521, 1295
PpsI GAGTC 4 cut(s) 404, 410, 521, 1295
Ppu21I YACGTR 1 cut(s) 1354
PpuMI RGGWCCY 1 cut(s) 886
Psp5II RGGWCCY 1 cut(s) 886
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspPI GGNCC 7 cut(s) 64, 269, 342, 359, 488, 886, 1139
PspPPI RGGWCCY 1 cut(s) 886
PstI CTGCAG 1 cut(s) 90
RsaI GTAC 2 cut(s) 740, 788
RsaNI GTAC 2 cut(s) 739, 787
SapI GCTCTTC 1 cut(s) 554
SaqAI TTAA 3 cut(s) 255, 891, 1179
SatI GCNGC 4 cut(s) 86, 89, 295, 405
Sau3AI GATC 3 cut(s) 744, 811, 977
Sau96I GGNCC 7 cut(s) 64, 269, 342, 359, 488, 886, 1139
SchI GAGTC 4 cut(s) 404, 410, 522, 1295
ScrFI CCNGG 1 cut(s) 68
SduI GDGCHC 2 cut(s) 33, 690
SfaNI GCATC 5 cut(s) 889, 904, 921, 1039, 1077
SfcI CTRYAG 1 cut(s) 86
SinI GGWCC 3 cut(s) 64, 342, 886
SmlI CTYRAG 2 cut(s) 240, 890
SmoI CTYRAG 2 cut(s) 240, 890
Sse9I AATT 4 cut(s) 960, 982, 1020, 1264
SspMI CTAG 2 cut(s) 950, 1295
StyD4I CCNGG 1 cut(s) 66
StyI CCWWGG 1 cut(s) 491
TaaI ACNGT 2 cut(s) 454, 625
TaiI ACGT 2 cut(s) 264, 1356
TaqI TCGA 3 cut(s) 249, 501, 980
TasI AATT 4 cut(s) 960, 982, 1020, 1264
TatI WGTACW 1 cut(s) 738
TauI GCSGC 2 cut(s) 297, 407
TfiI GAWTC 2 cut(s) 467, 505
Tru1I TTAA 3 cut(s) 255, 891, 1179
Tru9I TTAA 3 cut(s) 255, 891, 1179
TscAI CASTG 5 cut(s) 571, 811, 835, 1141, 1150
TseFI GTSAC 1 cut(s) 1240
TseI GCWGC 2 cut(s) 85, 88
Tsp45I GTSAC 1 cut(s) 1240
TspDTI ATGAA 9 cut(s) 17, 297, 591, 617, 668, 745, 773, 981, 1365
TspGWI ACGGA 1 cut(s) 953
TspRI CASTG 5 cut(s) 571, 811, 835, 1141, 1150
Vha464I CTTAAG 1 cut(s) 890
VneI GTGCAC 1 cut(s) 686
VpaK11BI GGWCC 3 cut(s) 64, 342, 886
XapI RAATTY 2 cut(s) 982, 1020
XbaI TCTAGA 1 cut(s) 1294
XceI RCATGY 1 cut(s) 929
XcmI CCANNNNNNNNNTGG 1 cut(s) 1006
XmiI GTMKAC 1 cut(s) 1072
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 2 cut(s) 950, 1295
ZraI GACGTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.