Rmu_ssc0000119.1_g000022

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000119.1
OG0007510
Physical Location & Seq
Reverse (-)
74209 .. 76501
2293 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1371 bp
atggtgtcctcctctcagaacaataacaaaaccctccattcccttcttgcctcaaacatcaccaagctcacaatcccaccttcctcctcctcctcttcttctccgcctcaagacttcgactttaacgacgtcttcggccccccaaccgcctccaacgacggcggcgcttccacttcattctcgccgtcgttactcgtcatccacaaccgctcccactccttcgtcggcccctccccgcgcttcactcccacctcctcctcctccctcctccccttcaccgccgccgactccgactccgaggaagacgaaaacgacgccgtttccgaaaccgtgaaagatgaggaatcggcggttcggaagttcgggccttgggatttcgagattctcagagtcgtcggcaagggcgcgttcgggaaggtgtttcaggtgaggaagaagagcagtgatggcgatgaagatggtgatgggctttatgccatgaaggtcatgaggaaggagactgttatcaagaagaaccatgtggattacatgaaggctgagagggacattctcaccaaagttgtgcacccttttattgttcagctccgctactcttttcagaccaagtgtaagctgtacttgatcatggatttcatgaatggagggcatttgttctatcatttgtaccggcaggggatattcagtgaggatcaggcgaggtcttacactgctgagatagtgtctgctgtttcacatcttcacaaatgcgggattgtgcatcgggaccttaagccggaaaatgttctcatggatgctgatgggcatgtgatgctgacggattttggattagcaaaggaaattgatgaagatagcagatcgaattcaatgtgtggaaccaccgaatacatggctcctgaaattctgctgtctaaaggccacaacaaagatgcagattggtggagcattggtatacttttgtatgagatgctaagtggtcagccaccatttacgcatgcaaatagaaagaaacttcaggagagaatcatcagagagaaagttaaacttccaccttaccttaccactgaagctcactctttgctcaagggattgctacaaaaggaaccatcccaaaggttaggcagtggccccagtggaggtgataatatcaaaagtcataaatggtttaaaacaatcaactggaagaagttggaggccagagaactgctcccaaagttcaagccagatgtgactggaaaagaatgcacggccaattttgaccggtgctggacaacaatgcctctagatgactcaccagctcctacaccaacagcgggtgagcattttcagggatatacttacgtggcacctaacacttggctttcatctgtataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

456

Amino Acids

50.93

Weight (kDa)

6.47

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 126 - 388 1.4e-73 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 127 - 371 3.9e-40 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 206 - 287 2.5e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 230 - 370 1e-09 Kinase-like
Pkinase_fungal PF17667 239 - 311 2e-08 Fungal protein kinase
Pkinase_C PF00433 409 - 446 1.8e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 132
AccB1I GGYRCC 1 cut(s) 1342
AccBSI CCGCTC 1 cut(s) 210
AccI GTMKAC 1 cut(s) 949
AccII CGCG 2 cut(s) 238, 407
AclWI GGATC 1 cut(s) 696
AcoI YGGCCR 1 cut(s) 1245
AcsI RAATTY 2 cut(s) 859, 897
AcuI CTGAAG 2 cut(s) 995, 1083
AcyI GRCGYC 2 cut(s) 129, 315
AfaI GTAC 2 cut(s) 617, 665
AfiI CCNNNNNNNGG 3 cut(s) 772, 1133, 1310
AflII CTTAAG 1 cut(s) 767
AgeI ACCGGT 1 cut(s) 1257
AgsI TTSAA 2 cut(s) 864, 1216
AluBI AGCT 5 cut(s) 67, 583, 613, 1067, 1295
AluI AGCT 5 cut(s) 67, 583, 613, 1067, 1295
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 1 cut(s) 491
Alw44I GTGCAC 1 cut(s) 563
AlwI GGATC 1 cut(s) 696
AoxI GGCC 7 cut(s) 136, 226, 365, 913, 1123, 1191, 1245
ApaLI GTGCAC 1 cut(s) 563
ApoI RAATTY 2 cut(s) 859, 897
AsiGI ACCGGT 1 cut(s) 1257
Asp700I GAANNNNTTC 1 cut(s) 780
AspLEI GCGC 3 cut(s) 167, 240, 407
AspS9I GGNCC 5 cut(s) 137, 227, 365, 763, 1124
AsuHPI GGTGA 8 cut(s) 52, 268, 439, 473, 544, 1148, 1281, 1325
AvaII GGWCC 1 cut(s) 763
BaeGI GKGCMC 1 cut(s) 567
BanI GGYRCC 1 cut(s) 1342
BbsI GAAGAC 2 cut(s) 124, 309
Bbv12I GWGCWC 1 cut(s) 567
BccI CCATC 5 cut(s) 440, 452, 458, 791, 1111
BceAI ACGGC 4 cut(s) 169, 175, 302, 1260
BclI TGATCA 1 cut(s) 621
BcoDI GTCTC 1 cut(s) 491
BfaI CTAG 1 cut(s) 1280
BfoI RGCGCY 1 cut(s) 168
BfrI CTTAAG 1 cut(s) 767
BisI GCNGC 2 cut(s) 163, 282
BlsI GCNGC 2 cut(s) 164, 283
Bme18I GGWCC 1 cut(s) 763
BmgT120I GGNCC 5 cut(s) 137, 227, 365, 763, 1124
BmiI GGNNCC 8 cut(s) 139, 229, 764, 874, 891, 1101, 1126, 1344
BmrI ACTGGG 1 cut(s) 1122
BmsI GCATC 5 cut(s) 766, 781, 798, 916, 954
BmuI ACTGGG 1 cut(s) 1122
BpiI GAAGAC 2 cut(s) 124, 309
BplI GAGNNNNNCTC 2 cut(s) 1188, 1220
BpuEI CTTGAG 2 cut(s) 93, 1064
BsaAI YACGTR 1 cut(s) 1339
BsaHI GRCGYC 2 cut(s) 129, 315
BsaJI CCNNGG 2 cut(s) 297, 368
BsaWI WCCGGW 1 cut(s) 1257
BsaXI ACNNNNNCTCC 6 cut(s) 194, 224, 278, 308, 931, 961
Bsc4I CCNNNNNNNGG 3 cut(s) 772, 1133, 1310
Bse118I RCCGGY 2 cut(s) 666, 1257
Bse1I ACTGG 3 cut(s) 1128, 1181, 1234
BseDI CCNNGG 2 cut(s) 297, 368
BseGI GGATG 3 cut(s) 198, 796, 1103
BseLI CCNNNNNNNGG 3 cut(s) 772, 1133, 1310
BseMII CTCAG 4 cut(s) 29, 400, 528, 702
BseNI ACTGG 3 cut(s) 1128, 1181, 1234
BseRI GAGGAG 7 cut(s) 76, 79, 82, 244, 247, 250, 257
BseSI GKGCMC 1 cut(s) 567
Bsh1236I CGCG 2 cut(s) 238, 407
BshFI GGCC 7 cut(s) 138, 228, 367, 915, 1125, 1193, 1247
BshNI GGYRCC 1 cut(s) 1342
BshTI ACCGGT 1 cut(s) 1257
BsiHKAI GWGCWC 1 cut(s) 567
BsiSI CCGG 3 cut(s) 667, 773, 1258
BslFI GGGAC 2 cut(s) 557, 776
BslI CCNNNNNNNGG 3 cut(s) 772, 1133, 1310
BsmAI GTCTC 1 cut(s) 491
BsmFI GGGAC 2 cut(s) 557, 776
BsmI GAATGC 1 cut(s) 1244
BsnI GGCC 7 cut(s) 138, 228, 367, 915, 1125, 1193, 1247
Bsp1286I GDGCHC 1 cut(s) 567
Bsp143I GATC 3 cut(s) 621, 688, 854
BspANI GGCC 7 cut(s) 138, 228, 367, 915, 1125, 1193, 1247
BspCNI CTCAG 4 cut(s) 28, 399, 529, 703
BspFNI CGCG 2 cut(s) 238, 407
BspHI TCATGA 2 cut(s) 486, 633
BspLI GGNNCC 8 cut(s) 139, 229, 764, 874, 891, 1101, 1126, 1344
BspPI GGATC 1 cut(s) 696
BspQI GCTCTTC 1 cut(s) 431
BspT107I GGYRCC 1 cut(s) 1342
BspTI CTTAAG 1 cut(s) 767
BsrBI CCGCTC 1 cut(s) 210
BsrFI RCCGGY 2 cut(s) 666, 1257
BsrI ACTGG 3 cut(s) 1128, 1181, 1234
BssAI RCCGGY 2 cut(s) 666, 1257
BssECI CCNNGG 2 cut(s) 297, 368
BssMI GATC 3 cut(s) 621, 688, 854
BssNAI GTATAC 1 cut(s) 950
BssNI GRCGYC 2 cut(s) 129, 315
BssT1I CCWWGG 1 cut(s) 368
Bst1107I GTATAC 1 cut(s) 950
Bst4CI ACNGT 2 cut(s) 331, 502
Bst6I CTCTTC 2 cut(s) 100, 431
BstACI GRCGYC 2 cut(s) 129, 315
BstAFI CTTAAG 1 cut(s) 767
BstAPI GCANNNNNTGC 1 cut(s) 808
BstBAI YACGTR 1 cut(s) 1339
BstC8I GCNNGC 1 cut(s) 993
BstDEI CTNAG 5 cut(s) 15, 386, 537, 711, 968
BstF5I GGATG 3 cut(s) 198, 796, 1103
BstFNI CGCG 2 cut(s) 238, 407
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 3 cut(s) 167, 240, 407
BstKTI GATC 3 cut(s) 624, 691, 857
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 3 cut(s) 621, 688, 854
BstMWI GCNNNNNNNGC 2 cut(s) 447, 808
BstNSI RCATGY 2 cut(s) 806, 995
BstSLI GKGCMC 1 cut(s) 567
BstUI CGCG 2 cut(s) 238, 407
BstV2I GAAGAC 2 cut(s) 124, 309
BstZ17I GTATAC 1 cut(s) 950
BsuRI GGCC 7 cut(s) 138, 228, 367, 915, 1125, 1193, 1247
BtgZI GCGATG 1 cut(s) 465
BtsCI GGATG 3 cut(s) 198, 796, 1103
BtsI GCAGTG 3 cut(s) 448, 705, 1126
BtsIMutI CAGTG 6 cut(s) 448, 688, 705, 1059, 1126, 1135
Cac8I GCNNGC 1 cut(s) 993
CciI TCATGA 2 cut(s) 486, 633
CfoI GCGC 3 cut(s) 167, 240, 407
Cfr10I RCCGGY 2 cut(s) 666, 1257
Cfr13I GGNCC 5 cut(s) 137, 227, 365, 763, 1124
CseI GACGC 1 cut(s) 323
Csp6I GTAC 2 cut(s) 616, 664
CspAI ACCGGT 1 cut(s) 1257
CviQI GTAC 2 cut(s) 616, 664
DdeI CTNAG 5 cut(s) 15, 386, 537, 711, 968
DpnI GATC 3 cut(s) 623, 690, 856
DpnII GATC 3 cut(s) 621, 688, 854
DraI TTTAAA 1 cut(s) 1165
EaeI YGGCCR 1 cut(s) 1245
Eam1104I CTCTTC 2 cut(s) 100, 431
EarI CTCTTC 2 cut(s) 100, 431
EciI GGCGGA 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 368
Eco47I GGWCC 1 cut(s) 763
Eco57I CTGAAG 2 cut(s) 995, 1083
EcoO109I RGGNCCY 1 cut(s) 763
EcoRI GAATTC 1 cut(s) 859
EcoT14I CCWWGG 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 368
FalI AAGNNNNNCTT 4 cut(s) 602, 634, 1026, 1058
FaqI GGGAC 2 cut(s) 557, 776
FauI CCCGC 3 cut(s) 243, 740, 1303
FbaI TGATCA 1 cut(s) 621
FblI GTMKAC 1 cut(s) 949
Fnu4HI GCNGC 2 cut(s) 163, 282
FokI GGATG 3 cut(s) 185, 803, 1090
Fsp4HI GCNGC 2 cut(s) 163, 282
FspBI CTAG 1 cut(s) 1280
GlaI GCGC 3 cut(s) 166, 239, 406
GluI GCNGC 2 cut(s) 163, 282
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 7 cut(s) 138, 228, 367, 915, 1125, 1193, 1247
HapII CCGG 3 cut(s) 667, 773, 1258
HgaI GACGC 1 cut(s) 323
HhaI GCGC 3 cut(s) 167, 240, 407
Hin1I GRCGYC 2 cut(s) 129, 315
Hin6I GCGC 3 cut(s) 165, 238, 405
HinP1I GCGC 3 cut(s) 165, 238, 405
HinfI GANTC 7 cut(s) 287, 293, 344, 382, 390, 1020, 1286
HpaII CCGG 3 cut(s) 667, 773, 1258
HphI GGTGA 8 cut(s) 52, 268, 439, 473, 544, 1148, 1281, 1325
Hpy166II GTNNAC 2 cut(s) 565, 950
Hpy188I TCNGA 8 cut(s) 18, 292, 298, 325, 357, 389, 600, 1028
Hpy8I GTNNAC 2 cut(s) 565, 950
Hpy99I CGWCG 6 cut(s) 131, 161, 190, 227, 317, 398
HpyAV CCTTC 8 cut(s) 53, 90, 229, 283, 409, 475, 487, 526
HpyCH4III ACNGT 2 cut(s) 331, 502
HpyCH4IV ACGT 2 cut(s) 129, 1338
HpyCH4V TGCA 5 cut(s) 565, 757, 929, 995, 1242
HpyF10VI GCNNNNNNNGC 2 cut(s) 447, 808
HpyF3I CTNAG 5 cut(s) 15, 386, 537, 711, 968
HpySE526I ACGT 2 cut(s) 129, 1338
Hsp92I GRCGYC 2 cut(s) 129, 315
HspAI GCGC 3 cut(s) 165, 238, 405
Ksp22I TGATCA 1 cut(s) 621
Kzo9I GATC 3 cut(s) 621, 688, 854
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 6 cut(s) 215, 588, 895, 939, 1209, 1300
LweI GCATC 5 cut(s) 766, 781, 798, 916, 954
MaeI CTAG 1 cut(s) 1280
MaeII ACGT 2 cut(s) 129, 1338
MaeIII GTNAC 2 cut(s) 189, 1225
MalI GATC 3 cut(s) 623, 690, 856
MbiI CCGCTC 1 cut(s) 210
MboI GATC 3 cut(s) 621, 688, 854
MhlI GDGCHC 1 cut(s) 567
MluCI AATT 4 cut(s) 837, 859, 897, 1249
MlyI GAGTC 4 cut(s) 281, 287, 399, 1280
MmeI TCCRAC 3 cut(s) 177, 315, 1167
MroXI GAANNNNTTC 1 cut(s) 780
MseI TTAA 4 cut(s) 123, 768, 1038, 1164
MspA1I CMGCKG 1 cut(s) 1310
MspCI CTTAAG 1 cut(s) 767
MspI CCGG 3 cut(s) 667, 773, 1258
Mva1269I GAATGC 1 cut(s) 1244
MvnI CGCG 2 cut(s) 238, 407
MwoI GCNNNNNNNGC 2 cut(s) 447, 808
NdeII GATC 3 cut(s) 621, 688, 854
NlaIV GGNNCC 8 cut(s) 139, 229, 764, 874, 891, 1101, 1126, 1344
NmuCI GTSAC 1 cut(s) 1225
NspI RCATGY 2 cut(s) 806, 995
PaeI GCATGC 1 cut(s) 995
PagI TCATGA 2 cut(s) 486, 633
PciSI GCTCTTC 1 cut(s) 431
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 321
PctI GAATGC 1 cut(s) 1244
PdmI GAANNNNTTC 1 cut(s) 780
PfeI GAWTC 3 cut(s) 344, 382, 1020
PinAI ACCGGT 1 cut(s) 1257
PkrI GCNGC 2 cut(s) 164, 283
PleI GAGTC 4 cut(s) 281, 287, 398, 1280
PpsI GAGTC 4 cut(s) 281, 287, 398, 1280
Ppu21I YACGTR 1 cut(s) 1339
PpuMI RGGWCCY 1 cut(s) 763
Psp5II RGGWCCY 1 cut(s) 763
PspN4I GGNNCC 8 cut(s) 139, 229, 764, 874, 891, 1101, 1126, 1344
PspPI GGNCC 5 cut(s) 137, 227, 365, 763, 1124
PspPPI RGGWCCY 1 cut(s) 763
RsaI GTAC 2 cut(s) 617, 665
RsaNI GTAC 2 cut(s) 616, 664
SapI GCTCTTC 1 cut(s) 431
SaqAI TTAA 4 cut(s) 123, 768, 1038, 1164
SatI GCNGC 2 cut(s) 163, 282
Sau3AI GATC 3 cut(s) 621, 688, 854
Sau96I GGNCC 5 cut(s) 137, 227, 365, 763, 1124
SchI GAGTC 4 cut(s) 281, 287, 399, 1280
SduI GDGCHC 1 cut(s) 567
SfaNI GCATC 5 cut(s) 766, 781, 798, 916, 954
SinI GGWCC 1 cut(s) 763
SmlI CTYRAG 3 cut(s) 108, 767, 1079
SmoI CTYRAG 3 cut(s) 108, 767, 1079
SphI GCATGC 1 cut(s) 995
Sse9I AATT 4 cut(s) 837, 859, 897, 1249
SspMI CTAG 1 cut(s) 1280
StyI CCWWGG 1 cut(s) 368
TaaI ACNGT 2 cut(s) 331, 502
TaiI ACGT 2 cut(s) 132, 1341
TaqI TCGA 3 cut(s) 117, 378, 857
TasI AATT 4 cut(s) 837, 859, 897, 1249
TatI WGTACW 1 cut(s) 615
TauI GCSGC 2 cut(s) 165, 284
TfiI GAWTC 3 cut(s) 344, 382, 1020
Tru1I TTAA 4 cut(s) 123, 768, 1038, 1164
Tru9I TTAA 4 cut(s) 123, 768, 1038, 1164
TscAI CASTG 6 cut(s) 448, 688, 712, 1066, 1126, 1135
TseFI GTSAC 1 cut(s) 1225
Tsp45I GTSAC 1 cut(s) 1225
TspDTI ATGAA 8 cut(s) 165, 468, 494, 545, 622, 650, 858, 1350
TspGWI ACGGA 1 cut(s) 830
TspRI CASTG 6 cut(s) 448, 688, 712, 1066, 1126, 1135
Vha464I CTTAAG 1 cut(s) 767
VneI GTGCAC 1 cut(s) 563
VpaK11BI GGWCC 1 cut(s) 763
XapI RAATTY 2 cut(s) 859, 897
XbaI TCTAGA 1 cut(s) 1279
XceI RCATGY 2 cut(s) 806, 995
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmiI GTMKAC 1 cut(s) 949
XmnI GAANNNNTTC 1 cut(s) 780
XspI CTAG 1 cut(s) 1280
ZraI GACGTC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.