Rmu_ssc0000157.1_g000005

Mitochondrial inner membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000157.1
OG0009287
Physical Location & Seq
Reverse (-)
26005 .. 29764
3760 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1194 bp
atggcttactggcggggcctctcgtccagagcgagcatcattgctcggcgaaagcagcaccatccctgcatctctcacattctccacgacgacgacgtcgtttcggaaccctctccttccccaaataccaatcatttcctcacccaaaagcggttcttcagtttggatagtaaagtttcaggctttggtgggtttgctcagaatcggaggatttcaaatgctctgctttcgtcgccgccggctggattcgggtttgctcggaatatgtcgacggcgggcgggggaggggcggataagattggggtgttcagtgatgtgccggagattctgtcggacacgacggtgcaagctgtgacttcgcagctgccggtggtgaatgaggtggcgattgcggcggctgattcctacttgccggttaagggcttgcagtacttgattgattatgtccatactactgctgggttgagctggtgggctgccattgtggtaacaactcttttgattcgaagctgcacggttccgcttctagtaaaccaacttaaagcaacggcaaaactcactcttatgaggccacgtctagaggaacttaagcaagagatggaagaaaagggtatggatctcggagctaagattgaatatcaaaagaaaattaagatgctattcaaggaatatggctgtagtcccttaactccactaaagggactctttattcaagctcctgtcttcatcagctttttcctagcaatatcgaacatggcagagaaggtgccatcgtttaaagatggtggtgcctattggtttgttgatctgacaactcctgatcctatgtacatttttccagttttggcaggattgacattcttaataactgtggaggctgtattttgttactgggttacctccaacttgttttcgctcggatatggaattgtaattaagaatcctttcgtgaagaagacactgggtcttcctcagctgcctaccccggctccacagccacaaggatcacaacaatctgcattctcactgttttcacaaatgaaacagctggctgcagccccagagcccgcctcatcaaccgttcaaccagcaaagattcaagaccggagaacatcatcgacatcagatctcagtcaaaggattaaaagtttagagaaaaaagtaaagggaaaaaagagaaacaagaagaggtag

Protein Analysis

397

Amino Acids

43.67

Weight (kDa)

9.66

Isoelectric Point (pI)

49.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
60KD_IMP PF02096 157 - 313 6.6e-19 60Kd inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
AatII GACGTC 1 cut(s) 99
AccB1I GGYRCC 2 cut(s) 766, 788
AccI GTMKAC 1 cut(s) 269
AclWI GGATC 3 cut(s) 624, 815, 1012
AcuI CTGAAG 1 cut(s) 142
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 2 cut(s) 431, 830
AfiI CCNNNNNNNGG 4 cut(s) 150, 242, 419, 698
AflII CTTAAG 1 cut(s) 587
AgsI TTSAA 6 cut(s) 216, 635, 664, 713, 1085, 1100
AhdI GACNNNNNGTC 1 cut(s) 963
AjiI CACGTC 1 cut(s) 575
AleI CACNNNNGTG 1 cut(s) 341
AluBI AGCT 9 cut(s) 350, 364, 468, 510, 626, 716, 732, 976, 1048
AluI AGCT 9 cut(s) 350, 364, 468, 510, 626, 716, 732, 976, 1048
AlwI GGATC 3 cut(s) 624, 815, 1012
AoxI GGCC 2 cut(s) 16, 569
ApeKI GCWGC 8 cut(s) 55, 361, 364, 476, 510, 976, 1052, 1055
Asp700I GAANNNNTTC 1 cut(s) 944
AspS9I GGNCC 1 cut(s) 16
AsuC2I CCSGG 1 cut(s) 986
AsuHPI GGTGA 2 cut(s) 133, 385
AsuII TTCGAA 1 cut(s) 505
BanI GGYRCC 2 cut(s) 766, 788
BanII GRGCYC 1 cut(s) 1068
BbsI GAAGAC 3 cut(s) 715, 959, 962
BbvCI CCTCAGC 1 cut(s) 972
BbvI GCAGC 8 cut(s) 67, 351, 373, 463, 497, 963, 1039, 1067
BccI CCATC 4 cut(s) 69, 592, 776, 778
BceAI ACGGC 2 cut(s) 288, 564
BcnI CCSGG 1 cut(s) 986
BfaI CTAG 3 cut(s) 527, 578, 740
BfmI CTRYAG 2 cut(s) 676, 1053
BfrI CTTAAG 1 cut(s) 587
BglII AGATCT 1 cut(s) 1126
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 1 cut(s) 986
BmeRI GACNNNNNGTC 1 cut(s) 963
BmgBI CACGTC 1 cut(s) 575
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 6 cut(s) 17, 108, 519, 768, 790, 990
BmrFI CCNGG 1 cut(s) 986
BmrI ACTGGG 2 cut(s) 901, 971
BmsI GCATC 3 cut(s) 45, 78, 645
BmuI ACTGGG 2 cut(s) 901, 971
BpiI GAAGAC 3 cut(s) 715, 959, 962
BplI GAGNNNNNCTC 2 cut(s) 1055, 1087
Bpu10I CCTNAGC 1 cut(s) 972
Bpu14I TTCGAA 1 cut(s) 505
BpuMI CCSGG 1 cut(s) 986
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 984
BsaWI WCCGGW 1 cut(s) 1104
BsaXI ACNNNNNCTCC 4 cut(s) 314, 344, 973, 1003
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 242, 419, 698
Bse118I RCCGGY 3 cut(s) 238, 367, 412
Bse1I ACTGG 4 cut(s) 14, 839, 896, 966
Bse3DI GCAATG 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 984
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 4 cut(s) 150, 242, 419, 698
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 3 cut(s) 212, 986, 1144
BseNI ACTGG 4 cut(s) 14, 839, 896, 966
BseXI GCAGC 8 cut(s) 67, 351, 373, 463, 497, 963, 1039, 1067
BseYI CCCAGC 1 cut(s) 458
BsgI GTGCAG 1 cut(s) 496
BshFI GGCC 2 cut(s) 18, 571
BshNI GGYRCC 2 cut(s) 766, 788
BsiSI CCGG 6 cut(s) 239, 320, 368, 413, 986, 1105
BslFI GGGAC 2 cut(s) 666, 714
BslI CCNNNNNNNGG 4 cut(s) 150, 242, 419, 698
BsmFI GGGAC 2 cut(s) 666, 714
BsmI GAATGC 1 cut(s) 1019
BsnI GGCC 2 cut(s) 18, 571
Bsp119I TTCGAA 1 cut(s) 505
Bsp1286I GDGCHC 1 cut(s) 1068
Bsp1407I TGTACA 1 cut(s) 828
Bsp143I GATC 5 cut(s) 616, 805, 820, 1004, 1126
BspANI GGCC 2 cut(s) 18, 571
BspCNI CTCAG 3 cut(s) 211, 985, 1143
BspLI GGNNCC 6 cut(s) 17, 108, 519, 768, 790, 990
BspMAI CTGCAG 1 cut(s) 1057
BspPI GGATC 3 cut(s) 624, 815, 1012
BspT104I TTCGAA 1 cut(s) 505
BspT107I GGYRCC 2 cut(s) 766, 788
BspTI CTTAAG 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 39
BsrFI RCCGGY 3 cut(s) 238, 367, 412
BsrGI TGTACA 1 cut(s) 828
BsrI ACTGG 4 cut(s) 14, 839, 896, 966
BssAI RCCGGY 3 cut(s) 238, 367, 412
BssECI CCNNGG 1 cut(s) 984
BssMI GATC 5 cut(s) 616, 805, 820, 1004, 1126
BssNI GRCGYC 1 cut(s) 96
Bst4CI ACNGT 5 cut(s) 343, 517, 871, 1029, 1081
Bst6I CTCTTC 1 cut(s) 1181
BstACI GRCGYC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 587
BstAUI TGTACA 1 cut(s) 828
BstBI TTCGAA 1 cut(s) 505
BstC8I GCNNGC 7 cut(s) 34, 240, 277, 348, 425, 1050, 1068
BstDEI CTNAG 4 cut(s) 198, 627, 972, 1130
BstEII GGTNACC 1 cut(s) 895
BstF5I GGATG 1 cut(s) 61
BstKTI GATC 5 cut(s) 619, 808, 823, 1007, 1129
BstMBI GATC 5 cut(s) 616, 805, 820, 1004, 1126
BstMWI GCNNNNNNNGC 3 cut(s) 55, 232, 392
BstPI GGTNACC 1 cut(s) 895
BstSCI CCNGG 1 cut(s) 984
BstSFI CTRYAG 2 cut(s) 676, 1053
BstV1I GCAGC 8 cut(s) 67, 351, 373, 463, 497, 963, 1039, 1067
BstV2I GAAGAC 3 cut(s) 715, 959, 962
BstX2I RGATCY 2 cut(s) 616, 1126
BstYI RGATCY 2 cut(s) 616, 1126
BsuRI GGCC 2 cut(s) 18, 571
BtrI CACGTC 1 cut(s) 575
BtsCI GGATG 1 cut(s) 61
BtsIMutI CAGTG 3 cut(s) 316, 959, 1025
Cac8I GCNNGC 7 cut(s) 34, 240, 277, 348, 425, 1050, 1068
Cfr10I RCCGGY 3 cut(s) 238, 367, 412
Cfr13I GGNCC 1 cut(s) 16
Csp6I GTAC 2 cut(s) 430, 829
CviAII CATG 1 cut(s) 754
CviQI GTAC 2 cut(s) 430, 829
DdeI CTNAG 4 cut(s) 198, 627, 972, 1130
DpnI GATC 5 cut(s) 618, 807, 822, 1006, 1128
DpnII GATC 5 cut(s) 616, 805, 820, 1004, 1126
DraI TTTAAA 1 cut(s) 778
DrdI GACNNNNNNGTC 1 cut(s) 95
DriI GACNNNNNGTC 1 cut(s) 963
DseDI GACNNNNNNGTC 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 1181
Eam1105I GACNNNNNGTC 1 cut(s) 963
EarI CTCTTC 1 cut(s) 1181
EciI GGCGGA 1 cut(s) 305
Eco24I GRGCYC 1 cut(s) 1068
Eco57I CTGAAG 1 cut(s) 142
Eco91I GGTNACC 1 cut(s) 895
EcoO109I RGGNCCY 1 cut(s) 16
EcoO65I GGTNACC 1 cut(s) 895
EcoT38I GRGCYC 1 cut(s) 1068
FaeI CATG 1 cut(s) 757
FaiI YATR 9 cut(s) 266, 444, 450, 566, 614, 672, 755, 827, 924
FalI AAGNNNNNCTT 4 cut(s) 140, 172, 689, 721
FaqI GGGAC 2 cut(s) 666, 714
FatI CATG 1 cut(s) 753
FauI CCCGC 4 cut(s) 6, 268, 272, 1075
FblI GTMKAC 1 cut(s) 269
FokI GGATG 1 cut(s) 48
FriOI GRGCYC 1 cut(s) 1068
FspBI CTAG 3 cut(s) 527, 578, 740
GsaI CCCAGC 1 cut(s) 462
HaeIII GGCC 2 cut(s) 18, 571
HapII CCGG 6 cut(s) 239, 320, 368, 413, 986, 1105
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 1 cut(s) 757
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HinfI GANTC 8 cut(s) 202, 246, 325, 401, 502, 702, 940, 1096
HpaII CCGG 6 cut(s) 239, 320, 368, 413, 986, 1105
HphI GGTGA 2 cut(s) 133, 385
Hpy166II GTNNAC 2 cut(s) 270, 532
Hpy188I TCNGA 9 cut(s) 106, 201, 207, 261, 334, 623, 810, 920, 1126
Hpy188III TCNNGA 5 cut(s) 27, 578, 818, 949, 1100
Hpy8I GTNNAC 2 cut(s) 270, 532
Hpy99I CGWCG 7 cut(s) 92, 95, 98, 101, 235, 274, 343
HpyAV CCTTC 2 cut(s) 126, 757
HpyCH4III ACNGT 5 cut(s) 343, 517, 871, 1029, 1081
HpyCH4IV ACGT 2 cut(s) 96, 574
HpyCH4V TGCA 6 cut(s) 69, 346, 427, 513, 1019, 1055
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 232, 392
HpyF3I CTNAG 4 cut(s) 198, 627, 972, 1130
HpySE526I ACGT 2 cut(s) 96, 574
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 1 cut(s) 757
KroI GCCGGC 1 cut(s) 238
KroNI GCCGGC 1 cut(s) 240
Kzo9I GATC 5 cut(s) 616, 805, 820, 1004, 1126
LmnI GCTCC 3 cut(s) 623, 721, 994
Lsp1109I GCAGC 8 cut(s) 67, 351, 373, 463, 497, 963, 1039, 1067
LweI GCATC 3 cut(s) 45, 78, 645
MaeI CTAG 3 cut(s) 527, 578, 740
MaeII ACGT 2 cut(s) 96, 574
MaeIII GTNAC 4 cut(s) 352, 487, 887, 895
MalI GATC 5 cut(s) 618, 807, 822, 1006, 1128
MboI GATC 5 cut(s) 616, 805, 820, 1004, 1126
MboII GAAGA 6 cut(s) 148, 614, 715, 959, 964, 967
MflI RGATCY 2 cut(s) 616, 1126
MhlI GDGCHC 1 cut(s) 1068
MluCI AATT 3 cut(s) 648, 927, 933
MlyI GAGTC 1 cut(s) 696
MmeI TCCRAC 2 cut(s) 312, 927
MroNI GCCGGC 1 cut(s) 238
MroXI GAANNNNTTC 1 cut(s) 944
MseI TTAA 9 cut(s) 417, 540, 588, 651, 686, 777, 863, 936, 1143
MslI CAYNNNNRTG 2 cut(s) 341, 563
MspA1I CMGCKG 3 cut(s) 364, 976, 1048
MspCI CTTAAG 1 cut(s) 587
MspI CCGG 6 cut(s) 239, 320, 368, 413, 986, 1105
MspR9I CCNGG 1 cut(s) 986
Mva1269I GAATGC 1 cut(s) 1019
MwoI GCNNNNNNNGC 3 cut(s) 55, 232, 392
NaeI GCCGGC 1 cut(s) 240
NciI CCSGG 1 cut(s) 986
NdeII GATC 5 cut(s) 616, 805, 820, 1004, 1126
NgoMIV GCCGGC 1 cut(s) 238
NlaIII CATG 1 cut(s) 757
NlaIV GGNNCC 6 cut(s) 17, 108, 519, 768, 790, 990
NmeAIII GCCGAG 1 cut(s) 25
NmuCI GTSAC 1 cut(s) 352
NspV TTCGAA 1 cut(s) 505
OliI CACNNNNGTG 1 cut(s) 341
PcsI WCGNNNNNNNCGW 3 cut(s) 29, 93, 96
PctI GAATGC 1 cut(s) 1019
PdiI GCCGGC 1 cut(s) 240
PdmI GAANNNNTTC 1 cut(s) 944
PfeI GAWTC 7 cut(s) 202, 246, 325, 401, 502, 940, 1096
PflFI GACNNNGTC 1 cut(s) 95
PleI GAGTC 1 cut(s) 696
PpsI GAGTC 1 cut(s) 696
PspEI GGTNACC 1 cut(s) 895
PspFI CCCAGC 1 cut(s) 458
PspN4I GGNNCC 6 cut(s) 17, 108, 519, 768, 790, 990
PspPI GGNCC 1 cut(s) 16
PstI CTGCAG 1 cut(s) 1057
PsuI RGATCY 2 cut(s) 616, 1126
PsyI GACNNNGTC 1 cut(s) 95
PvuII CAGCTG 3 cut(s) 364, 976, 1048
RsaI GTAC 2 cut(s) 431, 830
RsaNI GTAC 2 cut(s) 430, 829
RseI CAYNNNNRTG 2 cut(s) 341, 563
SalI GTCGAC 1 cut(s) 268
SaqAI TTAA 9 cut(s) 417, 540, 588, 651, 686, 777, 863, 936, 1143
Sau3AI GATC 5 cut(s) 616, 805, 820, 1004, 1126
Sau96I GGNCC 1 cut(s) 16
ScaI AGTACT 1 cut(s) 431
SchI GAGTC 1 cut(s) 696
ScrFI CCNGG 1 cut(s) 986
SduI GDGCHC 1 cut(s) 1068
SfaNI GCATC 3 cut(s) 45, 78, 645
SfcI CTRYAG 2 cut(s) 676, 1053
SfuI TTCGAA 1 cut(s) 505
SmiMI CAYNNNNRTG 2 cut(s) 341, 563
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
Sse9I AATT 3 cut(s) 648, 927, 933
SspMI CTAG 3 cut(s) 527, 578, 740
StyD4I CCNGG 1 cut(s) 984
TaaI ACNGT 5 cut(s) 343, 517, 871, 1029, 1081
TaiI ACGT 2 cut(s) 99, 577
TaqI TCGA 4 cut(s) 269, 505, 749, 1118
TasI AATT 3 cut(s) 648, 927, 933
TatI WGTACW 2 cut(s) 429, 828
TauI GCSGC 3 cut(s) 238, 395, 398
TfiI GAWTC 7 cut(s) 202, 246, 325, 401, 502, 940, 1096
Tru1I TTAA 9 cut(s) 417, 540, 588, 651, 686, 777, 863, 936, 1143
Tru9I TTAA 9 cut(s) 417, 540, 588, 651, 686, 777, 863, 936, 1143
TscAI CASTG 3 cut(s) 316, 966, 1032
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 8 cut(s) 55, 361, 364, 476, 510, 976, 1052, 1055
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 2 cut(s) 715, 1055
TspRI CASTG 3 cut(s) 316, 966, 1032
Tth111I GACNNNGTC 1 cut(s) 95
Vha464I CTTAAG 1 cut(s) 587
XbaI TCTAGA 1 cut(s) 577
XcmI CCANNNNNNNNNTGG 1 cut(s) 455
XmiI GTMKAC 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 944
XspI CTAG 3 cut(s) 527, 578, 740
ZraI GACGTC 1 cut(s) 97
ZrmI AGTACT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.