Rmu_ssc0000245.1_g000018

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000245.1
OG0008940
Physical Location & Seq
Reverse (-)
59681 .. 61687
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1224 bp
ctgcagcaccgatcccaaccgcctgcattccggcttccgatccagcctcctcaccggagcctcccagcctttgatctagcttctaagaccttggcgctgacgtggcgggcgaggtcgcggatcttggagggtgaaggtgaatcggaggacggagatggtgaatcggaatctggctggggagaacgagttggtgaagaggaagaagcagtgagttggagtaggaggaaggcgaggaagaagaagcggaggtcgcagactcagagatcgccgacgacagtggccggtgggtccgattcgaattccggcgagggctttccagaagcaaggggaggagagaggttgatgggagtcgagagagagagagagagtatgagggcaatattgtccatagatgttagattgggtggaattgctatatattggggccagaatgggaatgaaggcacattagcggaaacatgtgcttcagggaactacaaattcgtaaacatagctttcctctcatcatttggtaatggccaaacccctaccataaacctcgccggtcactgtgacccatccaccaacgaatgcaccaagttgagccccgagatcaagtcatgccaggccaaaggcattaaggtcatactctccataggaggagctgcaggaagctactctttggcttcatccgacgatgcccgccaagttgctacttacttgtggaacaacttcttgggaggacagtcctcttcaaggacgttgggagatgctgttttggatggagtcgactttgacattgaaggagggactgatcaatattgggatgacctcgcgaggtacctttccgggtacagcaagaagggcaagaaagtttacttgactgctgctccacagtgtccctttcctgatgcttatgttggaaatgcactcaacacaggcctctttgactttgtttgggtacaattctacaacaaccctccttgccagtacacttctggtgatatttcaaatctcgaagatggttggaagcaatggacttccgccattcctgcacataagattttcttaggacttcctgctgcaccccaagctgctggtagtggatttattccagcggctgatctcaactcacaagtccttccggctatcaagaattcagctaaatatggaggtgtcatgctttggtctaagtattatgatgatctagatggatacagctcttccatcaagaatgatgtctag

Protein Analysis

407

Amino Acids

44.08

Weight (kDa)

5.6

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 137 - 355 5.8e-22 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 819
AccB1I GGYRCC 1 cut(s) 819
AccI GTMKAC 1 cut(s) 768
AccII CGCG 2 cut(s) 118, 815
AciI CCGC 8 cut(s) 20, 106, 118, 244, 452, 682, 1023, 1097
AclWI GGATC 3 cut(s) 6, 34, 128
AcoI YGGCCR 2 cut(s) 279, 517
AcsI RAATTY 3 cut(s) 298, 479, 1135
AcuI CTGAAG 1 cut(s) 450
AfaI GTAC 4 cut(s) 821, 833, 942, 971
AfiI CCNNNNNNNGG 1 cut(s) 735
AflIII ACRYGT 1 cut(s) 458
AgsI TTSAA 3 cut(s) 735, 782, 990
AjiI CACGTC 1 cut(s) 102
AjnI CCWGG 1 cut(s) 603
AjuI GAANNNNNNNTTGG 2 cut(s) 698, 730
AluBI AGCT 7 cut(s) 80, 494, 644, 654, 1073, 1142, 1200
AluI AGCT 7 cut(s) 80, 494, 644, 654, 1073, 1142, 1200
AlwI GGATC 3 cut(s) 6, 34, 128
AlwNI CAGNNNCTG 1 cut(s) 1100
Ama87I CYCGRG 1 cut(s) 587
AoxI GGCC 5 cut(s) 279, 424, 517, 606, 919
ApeKI GCWGC 5 cut(s) 4, 644, 866, 1061, 1073
ApoI RAATTY 3 cut(s) 298, 479, 1135
Asp700I GAANNNNTTC 1 cut(s) 710
Asp718I GGTACC 1 cut(s) 819
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 2 cut(s) 288, 424
AsuC2I CCSGG 1 cut(s) 829
AsuHPI GGTGA 6 cut(s) 44, 143, 149, 170, 203, 992
AsuII TTCGAA 1 cut(s) 296
AvaI CYCGRG 1 cut(s) 587
AvaII GGWCC 1 cut(s) 288
BalI TGGCCA 1 cut(s) 519
BanI GGYRCC 1 cut(s) 819
BanII GRGCYC 1 cut(s) 587
BbvI GCAGC 5 cut(s) 16, 631, 853, 1048, 1060
BccI CCATC 7 cut(s) 149, 337, 565, 755, 995, 1184, 1214
BciT130I CCWGG 1 cut(s) 605
BciVI GTATCC 1 cut(s) 1187
BclI TGATCA 1 cut(s) 793
BcnI CCSGG 1 cut(s) 829
BfaI CTAG 3 cut(s) 77, 1187, 1222
BfmI CTRYAG 2 cut(s) 2, 645
BfoI RGCGCY 1 cut(s) 98
BfuI GTATCC 1 cut(s) 1187
BisI GCNGC 6 cut(s) 5, 645, 867, 1062, 1074, 1098
BlsI GCNGC 6 cut(s) 6, 646, 868, 1063, 1075, 1099
Bme1390I CCNGG 2 cut(s) 605, 829
Bme18I GGWCC 1 cut(s) 288
BmeT110I CYCGRG 1 cut(s) 587
BmgBI CACGTC 1 cut(s) 102
BmgT120I GGNCC 2 cut(s) 288, 424
BmiI GGNNCC 4 cut(s) 59, 289, 425, 821
BmrFI CCNGG 2 cut(s) 605, 829
BmsI GCATC 3 cut(s) 667, 739, 880
Bpu14I TTCGAA 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 829
BsaJI CCNNGG 1 cut(s) 90
BsaWI WCCGGW 1 cut(s) 54
BsaXI ACNNNNNCTCC 6 cut(s) 171, 201, 738, 768, 853, 883
Bsc4I CCNNNNNNNGG 1 cut(s) 735
Bse118I RCCGGY 2 cut(s) 281, 542
Bse1I ACTGG 1 cut(s) 967
Bse3DI GCAATG 1 cut(s) 1019
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 4 cut(s) 557, 668, 766, 811
BseLI CCNNNNNNNGG 1 cut(s) 735
BseMI GCAATG 1 cut(s) 1019
BseMII CTCAG 1 cut(s) 272
BseNI ACTGG 1 cut(s) 967
BseRI GAGGAG 3 cut(s) 39, 345, 654
BseXI GCAGC 5 cut(s) 16, 631, 853, 1048, 1060
BseYI CCCAGC 2 cut(s) 64, 174
BsgI GTGCAG 2 cut(s) 1017, 1047
Bsh1236I CGCG 2 cut(s) 118, 815
BshFI GGCC 5 cut(s) 281, 426, 519, 608, 921
BshNI GGYRCC 1 cut(s) 819
BsiHKCI CYCGRG 1 cut(s) 587
BsiSI CCGG 7 cut(s) 31, 55, 282, 303, 543, 828, 1124
BslFI GGGAC 2 cut(s) 802, 864
BslI CCNNNNNNNGG 1 cut(s) 735
BsmFI GGGAC 2 cut(s) 802, 864
BsmI GAATGC 2 cut(s) 26, 575
BsnI GGCC 5 cut(s) 281, 426, 519, 608, 921
BsoBI CYCGRG 1 cut(s) 587
Bsp119I TTCGAA 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 587
Bsp143I GATC 9 cut(s) 11, 39, 73, 120, 263, 591, 793, 1102, 1183
Bsp68I TCGCGA 1 cut(s) 815
BspACI CCGC 8 cut(s) 20, 106, 118, 244, 452, 682, 1023, 1097
BspANI GGCC 5 cut(s) 281, 426, 519, 608, 921
BspCNI CTCAG 1 cut(s) 271
BspFNI CGCG 2 cut(s) 118, 815
BspLI GGNNCC 4 cut(s) 59, 289, 425, 821
BspMAI CTGCAG 2 cut(s) 6, 649
BspPI GGATC 3 cut(s) 6, 34, 128
BspQI GCTCTTC 1 cut(s) 1207
BspT104I TTCGAA 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 819
BsrDI GCAATG 1 cut(s) 1019
BsrFI RCCGGY 2 cut(s) 281, 542
BsrI ACTGG 1 cut(s) 967
BssAI RCCGGY 2 cut(s) 281, 542
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 9 cut(s) 11, 39, 73, 120, 263, 591, 793, 1102, 1183
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 4 cut(s) 277, 551, 726, 876
Bst6I CTCTTC 3 cut(s) 189, 736, 1207
BstBI TTCGAA 1 cut(s) 296
BstC8I GCNNGC 3 cut(s) 24, 108, 682
BstDEI CTNAG 4 cut(s) 84, 258, 1048, 1170
BstENI CCTNNNNNAGG 1 cut(s) 733
BstF5I GGATG 4 cut(s) 557, 668, 766, 811
BstFNI CGCG 2 cut(s) 118, 815
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 9 cut(s) 14, 42, 76, 123, 266, 594, 796, 1105, 1186
BstMBI GATC 9 cut(s) 11, 39, 73, 120, 263, 591, 793, 1102, 1183
BstMWI GCNNNNNNNGC 5 cut(s) 103, 250, 843, 1031, 1070
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 1 cut(s) 462
BstSCI CCNGG 2 cut(s) 603, 827
BstSFI CTRYAG 2 cut(s) 2, 645
BstUI CGCG 2 cut(s) 118, 815
BstV1I GCAGC 5 cut(s) 16, 631, 853, 1048, 1060
BstX2I RGATCY 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 1076
BstYI RGATCY 1 cut(s) 120
BsuI GTATCC 1 cut(s) 1187
BsuRI GGCC 5 cut(s) 281, 426, 519, 608, 921
BtrI CACGTC 1 cut(s) 102
BtsCI GGATG 4 cut(s) 557, 668, 766, 811
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 4 cut(s) 213, 282, 547, 881
BtuMI TCGCGA 1 cut(s) 815
Cac8I GCNNGC 3 cut(s) 24, 108, 682
CaiI CAGNNNCTG 1 cut(s) 1100
CfoI GCGC 1 cut(s) 97
Cfr10I RCCGGY 2 cut(s) 281, 542
Cfr13I GGNCC 2 cut(s) 288, 424
Csp6I GTAC 4 cut(s) 820, 832, 941, 970
CviAII CATG 3 cut(s) 459, 600, 1159
CviQI GTAC 4 cut(s) 820, 832, 941, 970
DdeI CTNAG 4 cut(s) 84, 258, 1048, 1170
DpnI GATC 9 cut(s) 13, 41, 75, 122, 265, 593, 795, 1104, 1185
DpnII GATC 9 cut(s) 11, 39, 73, 120, 263, 591, 793, 1102, 1183
EaeI YGGCCR 2 cut(s) 279, 517
Eam1104I CTCTTC 3 cut(s) 189, 736, 1207
EarI CTCTTC 3 cut(s) 189, 736, 1207
EciI GGCGGA 1 cut(s) 1012
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 1 cut(s) 921
Eco24I GRGCYC 1 cut(s) 587
Eco47I GGWCC 1 cut(s) 288
Eco57I CTGAAG 1 cut(s) 450
Eco88I CYCGRG 1 cut(s) 587
EcoNI CCTNNNNNAGG 1 cut(s) 733
EcoRI GAATTC 2 cut(s) 298, 1135
EcoRII CCWGG 1 cut(s) 603
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 587
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 3 cut(s) 462, 603, 1162
FalI AAGNNNNNCTT 4 cut(s) 643, 675, 1031, 1063
FaqI GGGAC 2 cut(s) 802, 864
FatI CATG 3 cut(s) 458, 599, 1158
FauI CCCGC 2 cut(s) 99, 689
FbaI TGATCA 1 cut(s) 793
FblI GTMKAC 1 cut(s) 768
Fnu4HI GCNGC 6 cut(s) 5, 645, 867, 1062, 1074, 1098
FokI GGATG 4 cut(s) 544, 655, 773, 818
FriOI GRGCYC 1 cut(s) 587
Fsp4HI GCNGC 6 cut(s) 5, 645, 867, 1062, 1074, 1098
FspBI CTAG 3 cut(s) 77, 1187, 1222
GlaI GCGC 1 cut(s) 96
GluI GCNGC 6 cut(s) 5, 645, 867, 1062, 1074, 1098
GsaI CCCAGC 2 cut(s) 68, 178
HaeII RGCGCY 1 cut(s) 98
HaeIII GGCC 5 cut(s) 281, 426, 519, 608, 921
HapII CCGG 7 cut(s) 31, 55, 282, 303, 543, 828, 1124
HhaI GCGC 1 cut(s) 97
Hin1II CATG 3 cut(s) 462, 603, 1162
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 7 cut(s) 140, 161, 167, 256, 293, 348, 765
HpaII CCGG 7 cut(s) 31, 55, 282, 303, 543, 828, 1124
HphI GGTGA 6 cut(s) 44, 143, 149, 170, 203, 992
Hpy166II GTNNAC 4 cut(s) 487, 769, 856, 972
Hpy188I TCNGA 6 cut(s) 39, 145, 166, 261, 292, 673
Hpy188III TCNNGA 8 cut(s) 317, 352, 814, 887, 995, 1132, 1187, 1210
Hpy8I GTNNAC 4 cut(s) 487, 769, 856, 972
Hpy99I CGWCG 2 cut(s) 274, 677
HpyAV CCTTC 6 cut(s) 128, 220, 434, 776, 835, 1130
HpyCH4III ACNGT 4 cut(s) 277, 551, 726, 876
HpyCH4IV ACGT 2 cut(s) 101, 740
HpyCH4V TGCA 7 cut(s) 4, 26, 573, 647, 908, 1034, 1064
HpyF10VI GCNNNNNNNGC 5 cut(s) 103, 250, 843, 1031, 1070
HpyF3I CTNAG 4 cut(s) 84, 258, 1048, 1170
HpySE526I ACGT 2 cut(s) 101, 740
Hsp92II CATG 3 cut(s) 462, 603, 1162
HspAI GCGC 1 cut(s) 95
KpnI GGTACC 1 cut(s) 823
Ksp22I TGATCA 1 cut(s) 793
Kzo9I GATC 9 cut(s) 11, 39, 73, 120, 263, 591, 793, 1102, 1183
LguI GCTCTTC 1 cut(s) 1207
LmnI GCTCC 3 cut(s) 57, 641, 874
Lsp1109I GCAGC 5 cut(s) 16, 631, 853, 1048, 1060
LweI GCATC 3 cut(s) 667, 739, 880
MaeI CTAG 3 cut(s) 77, 1187, 1222
MaeII ACGT 2 cut(s) 101, 740
MaeIII GTNAC 2 cut(s) 545, 551
MalI GATC 9 cut(s) 13, 41, 75, 122, 265, 593, 795, 1104, 1185
MboI GATC 9 cut(s) 11, 39, 73, 120, 263, 591, 793, 1102, 1183
MboII GAAGA 7 cut(s) 206, 212, 247, 250, 723, 1010, 1194
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 1 cut(s) 587
MlsI TGGCCA 1 cut(s) 519
MluCI AATT 5 cut(s) 298, 408, 479, 944, 1135
MluNI TGGCCA 1 cut(s) 519
MlyI GAGTC 3 cut(s) 250, 357, 774
MmeI TCCRAC 4 cut(s) 194, 696, 880, 986
Mox20I TGGCCA 1 cut(s) 519
MroXI GAANNNNTTC 1 cut(s) 710
MscI TGGCCA 1 cut(s) 519
MseI TTAA 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 519
MspA1I CMGCKG 1 cut(s) 1097
MspI CCGG 7 cut(s) 31, 55, 282, 303, 543, 828, 1124
MspR9I CCNGG 2 cut(s) 605, 829
Mva1269I GAATGC 2 cut(s) 26, 575
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 2 cut(s) 118, 815
MwoI GCNNNNNNNGC 5 cut(s) 103, 250, 843, 1031, 1070
NciI CCSGG 1 cut(s) 829
NdeII GATC 9 cut(s) 11, 39, 73, 120, 263, 591, 793, 1102, 1183
NlaIII CATG 3 cut(s) 462, 603, 1162
NlaIV GGNNCC 4 cut(s) 59, 289, 425, 821
NmuCI GTSAC 2 cut(s) 545, 551
NruI TCGCGA 1 cut(s) 815
NspI RCATGY 1 cut(s) 462
NspV TTCGAA 1 cut(s) 296
PceI AGGCCT 1 cut(s) 921
PciI ACATGT 1 cut(s) 458
PciSI GCTCTTC 1 cut(s) 1207
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PctI GAATGC 2 cut(s) 26, 575
PdmI GAANNNNTTC 1 cut(s) 710
PfeI GAWTC 4 cut(s) 140, 161, 167, 293
PkrI GCNGC 6 cut(s) 6, 646, 868, 1063, 1075, 1099
PleI GAGTC 3 cut(s) 250, 356, 773
PpsI GAGTC 3 cut(s) 250, 356, 773
PscI ACATGT 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 603
PspFI CCCAGC 2 cut(s) 64, 174
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 4 cut(s) 59, 289, 425, 821
PspPI GGNCC 2 cut(s) 288, 424
PstI CTGCAG 2 cut(s) 6, 649
PstNI CAGNNNCTG 1 cut(s) 1100
PsuI RGATCY 1 cut(s) 120
RruI TCGCGA 1 cut(s) 815
RsaI GTAC 4 cut(s) 821, 833, 942, 971
RsaNI GTAC 4 cut(s) 820, 832, 941, 970
SalI GTCGAC 1 cut(s) 767
SapI GCTCTTC 1 cut(s) 1207
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 6 cut(s) 5, 645, 867, 1062, 1074, 1098
Sau3AI GATC 9 cut(s) 11, 39, 73, 120, 263, 591, 793, 1102, 1183
Sau96I GGNCC 2 cut(s) 288, 424
SchI GAGTC 3 cut(s) 250, 357, 774
ScrFI CCNGG 2 cut(s) 605, 829
SduI GDGCHC 1 cut(s) 587
SfaNI GCATC 3 cut(s) 667, 739, 880
SfcI CTRYAG 2 cut(s) 2, 645
SfuI TTCGAA 1 cut(s) 296
SinI GGWCC 1 cut(s) 288
Sse9I AATT 5 cut(s) 298, 408, 479, 944, 1135
SseBI AGGCCT 1 cut(s) 921
SsiI CCGC 8 cut(s) 20, 106, 118, 244, 452, 682, 1023, 1097
SspI AATATT 2 cut(s) 381, 800
SspMI CTAG 3 cut(s) 77, 1187, 1222
StuI AGGCCT 1 cut(s) 921
StyD4I CCNGG 2 cut(s) 603, 827
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 4 cut(s) 277, 551, 726, 876
TaiI ACGT 2 cut(s) 104, 743
TaqI TCGA 4 cut(s) 296, 351, 768, 996
TasI AATT 5 cut(s) 298, 408, 479, 944, 1135
TatI WGTACW 1 cut(s) 969
TauI GCSGC 1 cut(s) 1100
TfiI GAWTC 4 cut(s) 140, 161, 167, 293
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TscAI CASTG 4 cut(s) 213, 282, 554, 881
TseFI GTSAC 2 cut(s) 545, 551
TseI GCWGC 5 cut(s) 4, 644, 866, 1061, 1073
Tsp45I GTSAC 2 cut(s) 545, 551
TspDTI ATGAA 2 cut(s) 453, 657
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 4 cut(s) 213, 282, 554, 881
VpaK11BI GGWCC 1 cut(s) 288
XagI CCTNNNNNAGG 1 cut(s) 733
XapI RAATTY 3 cut(s) 298, 479, 1135
XbaI TCTAGA 1 cut(s) 1186
XceI RCATGY 1 cut(s) 462
XcmI CCANNNNNNNNNTGG 1 cut(s) 974
XmiI GTMKAC 1 cut(s) 768
XmnI GAANNNNTTC 1 cut(s) 710
XspI CTAG 3 cut(s) 77, 1187, 1222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.