Rmu_ssc0000250.1_g000017

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000250.1
OG0017901
Physical Location & Seq
Forward (+)
86300 .. 89829
3530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000250.1_g000017.1.cds

Sequence Viewer

Length: 681 bp
ctggtctgcgcatcgaagatctgctcgcgcctctcgtcgttttcgcctctgatcttcaccaagatgattgatgatcaccaactgggcataatagccaatgttcttggcatgttcatatttctcctgatgaaaacgagaacccttgattacgaagcaggcgacccgaaagcccgagagcacggacgaaaagcccaggaacaaaaacccgacaaaccccaacgcgatcgatatgtccgtctctatgcagaacgcgctccacgccgtacacgtgtccatccctttcaaaaccctcgccagcgccgttcccgccgagtccgcagacagcagcagtcgaaccagacctgcaacacctcgctgaacagagttgagcctctggaaatctcccaaaccgacgccaccatttcgaatagcgagtatatcgccactatcgcattcgctcccaccactaatctgccatgcttttcgaatcggggaggaggggtcggagtcgcgggaggtggtgagcatgaagacgaacgaggcgagggaggagctgaaggtggcgacgcggaagatgaggatgaggtagttgaagcggcggagcttgcggagggagacggtggactggaagttagggtgggtggggatttggtagggggtttcgccgccgttttggggatgagcggagcgcatggtggttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

24.58

Weight (kDa)

6.02

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12587
malus_domestica MD01G1125600.v1.1 MD07G1195100.v1.1 MD07G1195400.v1.1
prunus_persica Prupe.2G232600_v2.0.a1 Prupe.2G232600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0366451
rosa_multiflora Rmu_ssc0000250.1_g000017
rosa_roxburghii Rroxscaffold_4G00289930 Rroxscaffold_4G00290190
rosa_samantha Rh1DG331100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 10
Acc36I ACCTGC 1 cut(s) 350
AccBSI CCGCTC 1 cut(s) 663
AccII CGCG 5 cut(s) 28, 222, 252, 491, 548
AciI CCGC 9 cut(s) 307, 316, 491, 548, 575, 578, 587, 645, 663
AcuI CTGAAG 1 cut(s) 555
AcvI CACGTG 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 393
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 1 cut(s) 654
AflIII ACRYGT 2 cut(s) 266, 268
AgsI TTSAA 2 cut(s) 284, 572
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 2 cut(s) 533, 583
AluI AGCT 2 cut(s) 533, 583
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 2 cut(s) 242, 588
Ama87I CYCGRG 1 cut(s) 171
ApeKI GCWGC 1 cut(s) 325
AspLEI GCGC 5 cut(s) 11, 30, 254, 300, 670
AsuHPI GGTGA 3 cut(s) 49, 68, 512
AsuII TTCGAA 2 cut(s) 404, 464
AvaI CYCGRG 1 cut(s) 171
BbrPI CACGTG 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 337
BccI CCATC 1 cut(s) 282
BceAI ACGGC 3 cut(s) 246, 285, 632
BciT130I CCWGG 1 cut(s) 194
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 2 cut(s) 242, 588
BfoI RGCGCY 1 cut(s) 301
BfuAI ACCTGC 1 cut(s) 350
BglII AGATCT 1 cut(s) 18
BisI GCNGC 3 cut(s) 326, 576, 645
BlsI GCNGC 3 cut(s) 327, 577, 646
Bme1390I CCNGG 1 cut(s) 194
BmeT110I CYCGRG 1 cut(s) 171
BmrFI CCNGG 1 cut(s) 194
BmrI ACTGGG 1 cut(s) 92
BmsI GCATC 1 cut(s) 20
BmuI ACTGGG 1 cut(s) 92
BpiI GAAGAC 1 cut(s) 516
Bpu14I TTCGAA 2 cut(s) 404, 464
Bsa29I ATCGAT 1 cut(s) 226
BsaAI YACGTR 1 cut(s) 269
BsaHI GRCGYC 1 cut(s) 393
BsaJI CCNNGG 1 cut(s) 192
BsaXI ACNNNNNCTCC 2 cut(s) 465, 495
Bsc4I CCNNNNNNNGG 1 cut(s) 654
Bse1I ACTGG 2 cut(s) 87, 609
BseBI CCWGG 1 cut(s) 194
BseCI ATCGAT 1 cut(s) 226
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 3 cut(s) 274, 565, 663
BseLI CCNNNNNNNGG 1 cut(s) 654
BseNI ACTGG 2 cut(s) 87, 609
BseRI GAGGAG 2 cut(s) 489, 543
BseXI GCAGC 1 cut(s) 337
Bsh1236I CGCG 5 cut(s) 28, 222, 252, 491, 548
Bsh1285I CGRYCG 1 cut(s) 226
BshVI ATCGAT 1 cut(s) 226
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 171
BslI CCNNNNNNNGG 1 cut(s) 654
BsmAI GTCTC 2 cut(s) 242, 588
BsmBI CGTCTC 2 cut(s) 242, 588
BsmI GAATGC 1 cut(s) 431
BsoBI CYCGRG 1 cut(s) 171
Bsp119I TTCGAA 2 cut(s) 404, 464
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 4 cut(s) 18, 51, 73, 223
BspACI CCGC 9 cut(s) 307, 316, 491, 548, 575, 578, 587, 645, 663
BspDI ATCGAT 1 cut(s) 226
BspFNI CGCG 5 cut(s) 28, 222, 252, 491, 548
BspMI ACCTGC 1 cut(s) 350
BspT104I TTCGAA 2 cut(s) 404, 464
BsrBI CCGCTC 1 cut(s) 663
BsrI ACTGG 2 cut(s) 87, 609
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 4 cut(s) 18, 51, 73, 223
BssNI GRCGYC 1 cut(s) 393
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 1 cut(s) 599
BstACI GRCGYC 1 cut(s) 393
BstBAI YACGTR 1 cut(s) 269
BstBI TTCGAA 2 cut(s) 404, 464
BstC8I GCNNGC 4 cut(s) 26, 157, 296, 585
BstF5I GGATG 3 cut(s) 274, 565, 663
BstFNI CGCG 5 cut(s) 28, 222, 252, 491, 548
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 5 cut(s) 11, 30, 254, 300, 670
BstKTI GATC 4 cut(s) 21, 54, 76, 226
BstMAI GTCTC 2 cut(s) 242, 588
BstMBI GATC 4 cut(s) 18, 51, 73, 223
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 6 cut(s) 251, 258, 306, 315, 428, 584
BstNI CCWGG 1 cut(s) 194
BstNSI RCATGY 1 cut(s) 112
BstSCI CCNGG 1 cut(s) 192
BstUI CGCG 5 cut(s) 28, 222, 252, 491, 548
BstV1I GCAGC 1 cut(s) 337
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
Bsu15I ATCGAT 1 cut(s) 226
BsuTUI ATCGAT 1 cut(s) 226
BtsCI GGATG 3 cut(s) 274, 565, 663
BveI ACCTGC 1 cut(s) 350
Cac8I GCNNGC 4 cut(s) 26, 157, 296, 585
CfoI GCGC 5 cut(s) 11, 30, 254, 300, 670
ClaI ATCGAT 1 cut(s) 226
CseI GACGC 2 cut(s) 401, 554
Csp6I GTAC 1 cut(s) 264
CviAII CATG 4 cut(s) 109, 456, 506, 671
CviJI RGCY 6 cut(s) 95, 170, 191, 370, 533, 583
CviKI_1 RGCY 6 cut(s) 95, 170, 191, 370, 533, 583
CviQI GTAC 1 cut(s) 264
DpnI GATC 4 cut(s) 20, 53, 75, 225
DpnII GATC 4 cut(s) 18, 51, 73, 223
EciI GGCGGA 1 cut(s) 593
Eco57I CTGAAG 1 cut(s) 555
Eco72I CACGTG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 192
Esp3I CGTCTC 2 cut(s) 242, 588
FaeI CATG 4 cut(s) 112, 459, 509, 674
FaiI YATR 9 cut(s) 89, 110, 116, 231, 243, 417, 457, 507, 672
FatI CATG 4 cut(s) 108, 455, 505, 670
FauI CCCGC 2 cut(s) 314, 484
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 3 cut(s) 326, 576, 645
FokI GGATG 3 cut(s) 261, 572, 670
Fsp4HI GCNGC 3 cut(s) 326, 576, 645
FspI TGCGCA 1 cut(s) 10
GlaI GCGC 5 cut(s) 10, 29, 253, 299, 669
GluI GCNGC 3 cut(s) 326, 576, 645
HaeII RGCGCY 1 cut(s) 301
HgaI GACGC 2 cut(s) 401, 554
HhaI GCGC 5 cut(s) 11, 30, 254, 300, 670
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 4 cut(s) 112, 459, 509, 674
Hin6I GCGC 5 cut(s) 9, 28, 252, 298, 668
HinP1I GCGC 5 cut(s) 9, 28, 252, 298, 668
HinfI GANTC 3 cut(s) 312, 466, 486
HphI GGTGA 3 cut(s) 49, 68, 512
Hpy166II GTNNAC 2 cut(s) 266, 602
Hpy188I TCNGA 2 cut(s) 51, 485
Hpy188III TCNNGA 2 cut(s) 124, 374
Hpy8I GTNNAC 2 cut(s) 266, 602
Hpy99I CGWCG 3 cut(s) 40, 395, 548
HpyAV CCTTC 1 cut(s) 530
HpyCH4III ACNGT 1 cut(s) 599
HpyCH4IV ACGT 1 cut(s) 268
HpyCH4V TGCA 2 cut(s) 245, 345
HpyF10VI GCNNNNNNNGC 6 cut(s) 251, 258, 306, 315, 428, 584
HpySE526I ACGT 1 cut(s) 268
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 4 cut(s) 112, 459, 509, 674
HspAI GCGC 5 cut(s) 9, 28, 252, 298, 668
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 4 cut(s) 18, 51, 73, 223
LmnI GCTCC 5 cut(s) 259, 442, 530, 580, 665
Lsp1109I GCAGC 1 cut(s) 337
LweI GCATC 1 cut(s) 20
MaeII ACGT 1 cut(s) 268
MalI GATC 4 cut(s) 20, 53, 75, 225
MbiI CCGCTC 1 cut(s) 663
MboI GATC 4 cut(s) 18, 51, 73, 223
MboII GAAGA 4 cut(s) 28, 46, 521, 563
MflI RGATCY 1 cut(s) 18
MhlI GDGCHC 1 cut(s) 180
MlyI GAGTC 2 cut(s) 321, 495
MmeI TCCRAC 1 cut(s) 463
MslI CAYNNNNRTG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 194
Mva1269I GAATGC 1 cut(s) 431
MvaI CCWGG 1 cut(s) 194
MvnI CGCG 5 cut(s) 28, 222, 252, 491, 548
MwoI GCNNNNNNNGC 6 cut(s) 251, 258, 306, 315, 428, 584
NdeII GATC 4 cut(s) 18, 51, 73, 223
NlaIII CATG 4 cut(s) 112, 459, 509, 674
NmeAIII GCCGAG 1 cut(s) 335
NsbI TGCGCA 1 cut(s) 10
NspI RCATGY 1 cut(s) 112
NspV TTCGAA 2 cut(s) 404, 464
PcsI WCGNNNNNNNCGW 6 cut(s) 32, 156, 232, 265, 298, 519
PctI GAATGC 1 cut(s) 431
PfeI GAWTC 1 cut(s) 466
PkrI GCNGC 3 cut(s) 327, 577, 646
Ple19I CGATCG 1 cut(s) 226
PleI GAGTC 2 cut(s) 320, 494
PmaCI CACGTG 1 cut(s) 269
PmlI CACGTG 1 cut(s) 269
PpsI GAGTC 2 cut(s) 320, 494
Ppu21I YACGTR 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 192
PspCI CACGTG 1 cut(s) 269
PspGI CCWGG 1 cut(s) 192
PsuI RGATCY 1 cut(s) 18
PvuI CGATCG 1 cut(s) 226
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
RseI CAYNNNNRTG 1 cut(s) 62
SatI GCNGC 3 cut(s) 326, 576, 645
Sau3AI GATC 4 cut(s) 18, 51, 73, 223
SchI GAGTC 2 cut(s) 321, 495
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 1 cut(s) 180
SetI ASST 8 cut(s) 271, 344, 353, 499, 535, 541, 567, 585
SfaNI GCATC 1 cut(s) 20
SfuI TTCGAA 2 cut(s) 404, 464
SmiMI CAYNNNNRTG 1 cut(s) 62
SsiI CCGC 9 cut(s) 307, 316, 491, 548, 575, 578, 587, 645, 663
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 599
TaiI ACGT 1 cut(s) 271
TaqI TCGA 5 cut(s) 14, 226, 332, 404, 464
TauI GCSGC 2 cut(s) 578, 647
TfiI GAWTC 1 cut(s) 466
TseI GCWGC 1 cut(s) 325
TspDTI ATGAA 3 cut(s) 103, 143, 522
TspGWI ACGGA 2 cut(s) 195, 224
XceI RCATGY 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.