Rh1DG331100

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0017901
Physical Location & Seq
Forward (+)
55605182 .. 55608559
3378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG331100.1

Sequence Viewer

Length: 252 bp
ATGCTCTTGCAGCGGGATGACGGTGGCCATCGGGCAAATGGAGGCGGCGGTCTTGGAGAGGATAGTTGGTCGTGTCTGGTCTGCGCATCGAAGATCTGCTTGCGCCTCTCGTCGTTTTCGCCTCTGATCTTCACCAAGATGATTGATGATCACCAACTGGGCATAATAGCCAATGTTCTTGGCATGTTCATATTTCTCCTGGTTATTGCTTATCATTACGTGACGGCAGACCCAAAATATGAAGGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ost4

Protein Analysis

83

Amino Acids

9.1

Weight (kDa)

5.99

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ost4 PF10215 47 - 79 2.6e-14 Oligosaccaryltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12587
malus_domestica MD01G1125600.v1.1 MD07G1195100.v1.1 MD07G1195400.v1.1
prunus_persica Prupe.2G232600_v2.0.a1 Prupe.2G232600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0366451
rosa_multiflora Rmu_ssc0000250.1_g000017
rosa_roxburghii Rroxscaffold_4G00289930 Rroxscaffold_4G00290190
rosa_samantha Rh1DG331100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 85
AciI CCGC 3 cut(s) 13, 45, 48
AcoI YGGCCR 1 cut(s) 25
AjnI CCWGG 1 cut(s) 198
AoxI GGCC 2 cut(s) 25, 244
ApeKI GCWGC 1 cut(s) 10
AspLEI GCGC 2 cut(s) 86, 105
AsuHPI GGTGA 2 cut(s) 124, 143
BalI TGGCCA 1 cut(s) 27
BbvI GCAGC 1 cut(s) 22
BccI CCATC 1 cut(s) 36
BceAI ACGGC 1 cut(s) 240
BciT130I CCWGG 1 cut(s) 200
BclI TGATCA 1 cut(s) 148
BglII AGATCT 1 cut(s) 93
BisI GCNGC 2 cut(s) 11, 46
BlsI GCNGC 2 cut(s) 12, 47
Bme1390I CCNGG 1 cut(s) 200
BmrFI CCNGG 1 cut(s) 200
BmrI ACTGGG 1 cut(s) 167
BmsI GCATC 1 cut(s) 95
BmuI ACTGGG 1 cut(s) 167
BsaAI YACGTR 1 cut(s) 220
Bse1I ACTGG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 200
BseGI GGATG 1 cut(s) 22
BseNI ACTGG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 22
BshFI GGCC 2 cut(s) 27, 246
BsnI GGCC 2 cut(s) 27, 246
Bsp143I GATC 3 cut(s) 93, 126, 148
BspACI CCGC 3 cut(s) 13, 45, 48
BspANI GGCC 2 cut(s) 27, 246
BsrI ACTGG 1 cut(s) 162
BssMI GATC 3 cut(s) 93, 126, 148
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 220
BstC8I GCNNGC 1 cut(s) 101
BstF5I GGATG 1 cut(s) 22
BstHHI GCGC 2 cut(s) 86, 105
BstKTI GATC 3 cut(s) 96, 129, 151
BstMBI GATC 3 cut(s) 93, 126, 148
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 198
BstV1I GCAGC 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 93
BstYI RGATCY 1 cut(s) 93
BsuRI GGCC 2 cut(s) 27, 246
BtsCI GGATG 1 cut(s) 22
BtsIMutI CAGTG 1 cut(s) 247
Cac8I GCNNGC 1 cut(s) 101
CfoI GCGC 2 cut(s) 86, 105
CviAII CATG 1 cut(s) 184
CviJI RGCY 3 cut(s) 27, 170, 246
CviKI_1 RGCY 3 cut(s) 27, 170, 246
DpnI GATC 3 cut(s) 95, 128, 150
DpnII GATC 3 cut(s) 93, 126, 148
EaeI YGGCCR 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 198
FaeI CATG 1 cut(s) 187
FaiI YATR 4 cut(s) 164, 185, 191, 240
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FatI CATG 1 cut(s) 183
FauI CCCGC 1 cut(s) 6
FbaI TGATCA 1 cut(s) 148
Fnu4HI GCNGC 2 cut(s) 11, 46
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 2 cut(s) 11, 46
FspI TGCGCA 1 cut(s) 85
GlaI GCGC 2 cut(s) 85, 104
GluI GCNGC 2 cut(s) 11, 46
HaeIII GGCC 2 cut(s) 27, 246
HhaI GCGC 2 cut(s) 86, 105
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 2 cut(s) 84, 103
HinP1I GCGC 2 cut(s) 84, 103
HphI GGTGA 2 cut(s) 124, 143
Hpy188I TCNGA 1 cut(s) 126
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 1 cut(s) 236
HpyCH4III ACNGT 1 cut(s) 23
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 1 cut(s) 10
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 2 cut(s) 84, 103
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 3 cut(s) 93, 126, 148
LpnPI CCDG 4 cut(s) 62, 143, 185, 212
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 1 cut(s) 95
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 1 cut(s) 220
MalI GATC 3 cut(s) 95, 128, 150
MboI GATC 3 cut(s) 93, 126, 148
MboII GAAGA 2 cut(s) 103, 121
MflI RGATCY 1 cut(s) 93
MlsI TGGCCA 1 cut(s) 27
MluNI TGGCCA 1 cut(s) 27
MnlI CCTC 4 cut(s) 35, 52, 116, 132
Mox20I TGGCCA 1 cut(s) 27
MscI TGGCCA 1 cut(s) 27
MslI CAYNNNNRTG 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 27
MspA1I CMGCKG 1 cut(s) 13
MspR9I CCNGG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 200
MwoI GCNNNNNNNGC 1 cut(s) 10
NdeII GATC 3 cut(s) 93, 126, 148
NlaIII CATG 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 220
NsbI TGCGCA 1 cut(s) 85
NspI RCATGY 1 cut(s) 187
PkrI GCNGC 2 cut(s) 12, 47
Ppu21I YACGTR 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PsuI RGATCY 1 cut(s) 93
RseI CAYNNNNRTG 1 cut(s) 137
SatI GCNGC 2 cut(s) 11, 46
Sau3AI GATC 3 cut(s) 93, 126, 148
ScrFI CCNGG 1 cut(s) 200
SetI ASST 1 cut(s) 222
SfaNI GCATC 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 137
SsiI CCGC 3 cut(s) 13, 45, 48
StyD4I CCNGG 1 cut(s) 198
TaaI ACNGT 1 cut(s) 23
TaiI ACGT 1 cut(s) 222
TaqI TCGA 1 cut(s) 89
TauI GCSGC 1 cut(s) 48
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 10
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 178
XceI RCATGY 1 cut(s) 187
XcmI CCANNNNNNNNNTGG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.