Rroxscaffold_4G00289930

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0017901
Physical Location & Seq
Reverse (-)
10535323 .. 10538606
3284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00289930.1

Sequence Viewer

Length: 258 bp
ATGGACTATATTGGTCAAGGGAAGTCTGTGCACGACTTGGCAGAAGAGATATATTTGCAGCGTCAGATCCAACGCACTACTCTGGTCTGCGCATCGAAGATCTGCTCGCGCGTCTCGTCGTTTTCGCCGCTGATCTTCACCAAGATGATTGATGATCACCAACTGGGCATAATAGCCAATGTTCTTGGCATGTTCATATTTGTCCTGGTGATTGCTTATCATTACGTGACGGCAGACCCAAAATATGAAGGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ost4

Protein Analysis

85

Amino Acids

9.65

Weight (kDa)

6.38

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ost4 PF10215 49 - 81 2.6e-14 Oligosaccaryltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12587
malus_domestica MD01G1125600.v1.1 MD07G1195100.v1.1 MD07G1195400.v1.1
prunus_persica Prupe.2G232600_v2.0.a1 Prupe.2G232600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0366451
rosa_multiflora Rmu_ssc0000250.1_g000017
rosa_roxburghii Rroxscaffold_4G00289930 Rroxscaffold_4G00290190
rosa_samantha Rh1DG331100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 91
AccII CGCG 2 cut(s) 109, 111
AciI CCGC 1 cut(s) 128
AclWI GGATC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 204
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 118
Alw44I GTGCAC 1 cut(s) 29
AlwI GGATC 1 cut(s) 61
AoxI GGCC 1 cut(s) 250
ApaLI GTGCAC 1 cut(s) 29
ApeKI GCWGC 1 cut(s) 58
AspLEI GCGC 2 cut(s) 92, 111
AsuHPI GGTGA 3 cut(s) 130, 149, 220
BaeGI GKGCMC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 1 cut(s) 70
BceAI ACGGC 1 cut(s) 246
BciT130I CCWGG 1 cut(s) 206
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 1 cut(s) 118
BglII AGATCT 1 cut(s) 99
BisI GCNGC 2 cut(s) 59, 128
BlsI GCNGC 2 cut(s) 60, 129
Bme1390I CCNGG 1 cut(s) 206
BmrFI CCNGG 1 cut(s) 206
BmrI ACTGGG 1 cut(s) 173
BmsI GCATC 1 cut(s) 101
BmuI ACTGGG 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 206
BseNI ACTGG 1 cut(s) 168
BseSI GKGCMC 1 cut(s) 33
BseXI GCAGC 1 cut(s) 70
Bsh1236I CGCG 2 cut(s) 109, 111
BshFI GGCC 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 118
BsmBI CGTCTC 1 cut(s) 118
BsnI GGCC 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 4 cut(s) 66, 99, 132, 154
BspACI CCGC 1 cut(s) 128
BspANI GGCC 1 cut(s) 252
BspFNI CGCG 2 cut(s) 109, 111
BspPI GGATC 1 cut(s) 61
BsrI ACTGG 1 cut(s) 168
BssMI GATC 4 cut(s) 66, 99, 132, 154
Bst2UI CCWGG 1 cut(s) 206
Bst6I CTCTTC 1 cut(s) 39
BstBAI YACGTR 1 cut(s) 226
BstC8I GCNNGC 1 cut(s) 107
BstFNI CGCG 2 cut(s) 109, 111
BstHHI GCGC 2 cut(s) 92, 111
BstKTI GATC 4 cut(s) 69, 102, 135, 157
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 4 cut(s) 66, 99, 132, 154
BstNI CCWGG 1 cut(s) 206
BstNSI RCATGY 1 cut(s) 193
BstSCI CCNGG 1 cut(s) 204
BstSLI GKGCMC 1 cut(s) 33
BstUI CGCG 2 cut(s) 109, 111
BstV1I GCAGC 1 cut(s) 70
BstX2I RGATCY 2 cut(s) 66, 99
BstYI RGATCY 2 cut(s) 66, 99
BsuRI GGCC 1 cut(s) 252
BtsIMutI CAGTG 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 107
CfoI GCGC 2 cut(s) 92, 111
CseI GACGC 2 cut(s) 50, 100
CviAII CATG 1 cut(s) 190
CviJI RGCY 2 cut(s) 176, 252
CviKI_1 RGCY 2 cut(s) 176, 252
DpnI GATC 4 cut(s) 68, 101, 134, 156
DpnII GATC 4 cut(s) 66, 99, 132, 154
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EcoRII CCWGG 1 cut(s) 204
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 1 cut(s) 193
FaiI YATR 6 cut(s) 9, 52, 170, 191, 197, 246
FatI CATG 1 cut(s) 189
FbaI TGATCA 1 cut(s) 154
Fnu4HI GCNGC 2 cut(s) 59, 128
Fsp4HI GCNGC 2 cut(s) 59, 128
FspI TGCGCA 1 cut(s) 91
GlaI GCGC 2 cut(s) 91, 110
GluI GCNGC 2 cut(s) 59, 128
HaeIII GGCC 1 cut(s) 252
HgaI GACGC 2 cut(s) 50, 100
HhaI GCGC 2 cut(s) 92, 111
Hin1II CATG 1 cut(s) 193
Hin6I GCGC 2 cut(s) 90, 109
HinP1I GCGC 2 cut(s) 90, 109
HphI GGTGA 3 cut(s) 130, 149, 220
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 1 cut(s) 66
Hpy8I GTNNAC 1 cut(s) 31
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 2 cut(s) 31, 58
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 1 cut(s) 193
HspAI GCGC 2 cut(s) 90, 109
Ksp22I TGATCA 1 cut(s) 154
Kzo9I GATC 4 cut(s) 66, 99, 132, 154
LpnPI CCDG 4 cut(s) 68, 149, 191, 218
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 1 cut(s) 101
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 226
MalI GATC 4 cut(s) 68, 101, 134, 156
MboI GATC 4 cut(s) 66, 99, 132, 154
MboII GAAGA 3 cut(s) 56, 109, 127
MflI RGATCY 2 cut(s) 66, 99
MhlI GDGCHC 1 cut(s) 33
MmeI TCCRAC 1 cut(s) 94
MslI CAYNNNNRTG 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 130
MspR9I CCNGG 1 cut(s) 206
MvaI CCWGG 1 cut(s) 206
MvnI CGCG 2 cut(s) 109, 111
NdeII GATC 4 cut(s) 66, 99, 132, 154
NlaIII CATG 1 cut(s) 193
NmuCI GTSAC 1 cut(s) 226
NsbI TGCGCA 1 cut(s) 91
NspI RCATGY 1 cut(s) 193
PcsI WCGNNNNNNNCGW 1 cut(s) 113
PkrI GCNGC 2 cut(s) 60, 129
Ppu21I YACGTR 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 204
PspGI CCWGG 1 cut(s) 204
PsuI RGATCY 2 cut(s) 66, 99
RseI CAYNNNNRTG 1 cut(s) 143
SatI GCNGC 2 cut(s) 59, 128
Sau3AI GATC 4 cut(s) 66, 99, 132, 154
ScrFI CCNGG 1 cut(s) 206
SduI GDGCHC 1 cut(s) 33
SetI ASST 1 cut(s) 228
SfaNI GCATC 1 cut(s) 101
SmiMI CAYNNNNRTG 1 cut(s) 143
SsiI CCGC 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 204
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 95
TauI GCSGC 1 cut(s) 130
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 1 cut(s) 58
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 184
VneI GTGCAC 1 cut(s) 29
XceI RCATGY 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.