Rmu_ssc0000250.1_g000020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000250.1
OG0011744
Physical Location & Seq
Forward (+)
109790 .. 110641
852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000250.1_g000020.1.cds

Sequence Viewer

Length: 708 bp
atgaaatctttgagtagtgtaggacttggtttgagtgtggtttttggttgcttattcttggctctaattgctgagctttactacttgctgtggtggaagaagaggctgaccaacagagagatagaagatgactacagcagcccatcaaaggaggtgttctacatgttctgctggagaaggacttccaagagaggtgttggtgatggtaccttgcttatcacagtggagacaccatatttgactcctttgggctctccaactcttttcacacctcctctcactcctgtggactctcataaccaacatggattcaatttcagttcccctctctttgaatcaaagagtgatgcagagttcaacaggataaggtcatcacctcctccaaagttcaagttcttgcaggaagcagaggagaaacttagagtgaaattaaaggaacaagctgaggagggtcttcagaagaaagatggacctgttgaagagaaagagagtaaagctagtaaagcttcaatgtcaaagttccttaaagatgaggatcaagatgggtcttatatcaccataattgttgacaagaacaaagaaagggagctcaatcaccatcttcatcatctacaacaacaccattcaagcacttcacaggtactcccactagtttcttcaccttcagcattcagatcatcaatcaacaagaagcccactttacattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

235

Amino Acids

26.77

Weight (kDa)

8.71

Isoelectric Point (pI)

63.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 206
AccB1I GGYRCC 1 cut(s) 206
AclWI GGATC 1 cut(s) 543
AcuI CTGAAG 2 cut(s) 440, 648
AfaI GTAC 2 cut(s) 208, 642
AfiI CCNNNNNNNGG 1 cut(s) 148
AflIII ACRYGT 1 cut(s) 162
AgsI TTSAA 7 cut(s) 313, 335, 358, 391, 479, 510, 627
AhlI ACTAGT 1 cut(s) 649
AleI CACNNNNGTG 1 cut(s) 284
AluBI AGCT 5 cut(s) 76, 443, 497, 506, 589
AluI AGCT 5 cut(s) 76, 443, 497, 506, 589
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 1 cut(s) 543
ApeKI GCWGC 1 cut(s) 138
ArsI GACNNNNNNTTYG 2 cut(s) 220, 252
Asp700I GAANNNNTTC 1 cut(s) 181
Asp718I GGTACC 1 cut(s) 206
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 5 cut(s) 212, 366, 547, 587, 651
AvaII GGWCC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 2 cut(s) 254, 591
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 591
BbvCI CCTCAGC 1 cut(s) 444
BbvI GCAGC 1 cut(s) 150
BccI CCATC 5 cut(s) 151, 197, 461, 536, 606
BcoDI GTCTC 1 cut(s) 221
BcuI ACTAGT 1 cut(s) 649
BfaI CTAG 2 cut(s) 498, 650
BfmI CTRYAG 1 cut(s) 133
BisI GCNGC 1 cut(s) 139
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 1 cut(s) 140
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 1 cut(s) 208
BmsI GCATC 1 cut(s) 337
BpiI GAAGAC 1 cut(s) 446
BpmI CTGGAG 1 cut(s) 193
Bpu10I CCTNAGC 1 cut(s) 444
Bpu1102I GCTNAGC 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 534
BsaXI ACNNNNNCTCC 2 cut(s) 627, 657
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse8I GATNNNNATC 1 cut(s) 534
BseJI GATNNNNATC 1 cut(s) 534
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMII CTCAG 2 cut(s) 63, 435
BseRI GAGGAG 4 cut(s) 264, 369, 425, 461
BseXI GCAGC 1 cut(s) 150
BshNI GGYRCC 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 591
BslI CCNNNNNNNGG 1 cut(s) 148
BsmAI GTCTC 1 cut(s) 221
BsmI GAATGC 1 cut(s) 668
Bsp1286I GDGCHC 2 cut(s) 254, 591
Bsp143I GATC 2 cut(s) 535, 674
Bsp1720I GCTNAGC 1 cut(s) 72
BspCNI CTCAG 2 cut(s) 64, 436
BspLI GGNNCC 1 cut(s) 208
BspPI GGATC 1 cut(s) 543
BspT107I GGYRCC 1 cut(s) 206
BssMI GATC 2 cut(s) 535, 674
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 2 cut(s) 95, 474
BstDEI CTNAG 3 cut(s) 72, 419, 444
BstKTI GATC 2 cut(s) 538, 677
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 2 cut(s) 535, 674
BstMWI GCNNNNNNNGC 2 cut(s) 68, 503
BstNSI RCATGY 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 133
BstV1I GCAGC 1 cut(s) 150
BstV2I GAAGAC 1 cut(s) 446
BtsIMutI CAGTG 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 470
Csp6I GTAC 2 cut(s) 207, 641
CviAII CATG 2 cut(s) 163, 305
CviQI GTAC 2 cut(s) 207, 641
DdeI CTNAG 3 cut(s) 72, 419, 444
DpnI GATC 2 cut(s) 537, 676
DpnII GATC 2 cut(s) 535, 674
Eam1104I CTCTTC 2 cut(s) 95, 474
EarI CTCTTC 2 cut(s) 95, 474
Ecl136II GAGCTC 1 cut(s) 589
Eco24I GRGCYC 2 cut(s) 254, 591
Eco47I GGWCC 1 cut(s) 470
Eco53kI GAGCTC 1 cut(s) 589
Eco57I CTGAAG 2 cut(s) 440, 648
EcoICRI GAGCTC 1 cut(s) 589
EcoT38I GRGCYC 2 cut(s) 254, 591
FaeI CATG 2 cut(s) 166, 308
FaiI YATR 6 cut(s) 164, 235, 297, 306, 552, 560
FatI CATG 2 cut(s) 162, 304
Fnu4HI GCNGC 1 cut(s) 139
FriOI GRGCYC 2 cut(s) 254, 591
Fsp4HI GCNGC 1 cut(s) 139
FspBI CTAG 2 cut(s) 498, 650
GluI GCNGC 1 cut(s) 139
GsuI CTGGAG 1 cut(s) 193
Hin1II CATG 2 cut(s) 166, 308
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 1 cut(s) 504
HinfI GANTC 4 cut(s) 241, 290, 309, 335
HphI GGTGA 5 cut(s) 212, 366, 547, 587, 651
Hpy166II GTNNAC 2 cut(s) 289, 568
Hpy188I TCNGA 2 cut(s) 459, 674
Hpy188III TCNNGA 1 cut(s) 539
Hpy8I GTNNAC 2 cut(s) 289, 568
HpyAV CCTTC 2 cut(s) 171, 672
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4V TGCA 2 cut(s) 350, 400
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 503
HpyF3I CTNAG 3 cut(s) 72, 419, 444
Hsp92II CATG 2 cut(s) 166, 308
KpnI GGTACC 1 cut(s) 210
Kzo9I GATC 2 cut(s) 535, 674
LmnI GCTCC 1 cut(s) 586
LpnPI CCDG 6 cut(s) 157, 297, 346, 386, 486, 623
Lsp1109I GCAGC 1 cut(s) 150
LweI GCATC 1 cut(s) 337
MaeI CTAG 2 cut(s) 498, 650
MalI GATC 2 cut(s) 537, 676
MboI GATC 2 cut(s) 535, 674
MboII GAAGA 8 cut(s) 109, 112, 137, 446, 472, 491, 593, 648
MhlI GDGCHC 2 cut(s) 254, 591
MluCI AATT 4 cut(s) 66, 313, 428, 561
MlyI GAGTC 2 cut(s) 235, 284
MmeI TCCRAC 1 cut(s) 281
MroXI GAANNNNTTC 1 cut(s) 181
MseI TTAA 2 cut(s) 431, 525
MslI CAYNNNNRTG 1 cut(s) 284
Mva1269I GAATGC 1 cut(s) 668
MwoI GCNNNNNNNGC 2 cut(s) 68, 503
NdeII GATC 2 cut(s) 535, 674
NlaIII CATG 2 cut(s) 166, 308
NlaIV GGNNCC 1 cut(s) 208
NspI RCATGY 1 cut(s) 166
OliI CACNNNNGTG 1 cut(s) 284
PciI ACATGT 1 cut(s) 162
PctI GAATGC 1 cut(s) 668
PdmI GAANNNNTTC 1 cut(s) 181
PfeI GAWTC 2 cut(s) 309, 335
PkrI GCNGC 1 cut(s) 140
PleI GAGTC 2 cut(s) 235, 284
PpsI GAGTC 2 cut(s) 235, 284
PscI ACATGT 1 cut(s) 162
Psp124BI GAGCTC 1 cut(s) 591
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 1 cut(s) 470
RsaI GTAC 2 cut(s) 208, 642
RsaNI GTAC 2 cut(s) 207, 641
RseI CAYNNNNRTG 1 cut(s) 284
SacI GAGCTC 1 cut(s) 591
SaqAI TTAA 2 cut(s) 431, 525
SatI GCNGC 1 cut(s) 139
Sau3AI GATC 2 cut(s) 535, 674
Sau96I GGNCC 1 cut(s) 470
SchI GAGTC 2 cut(s) 235, 284
SduI GDGCHC 2 cut(s) 254, 591
SfaNI GCATC 1 cut(s) 337
SfcI CTRYAG 1 cut(s) 133
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 1 cut(s) 284
SpeI ACTAGT 1 cut(s) 649
Sse9I AATT 4 cut(s) 66, 313, 428, 561
SspMI CTAG 2 cut(s) 498, 650
SstI GAGCTC 1 cut(s) 591
TaaI ACNGT 1 cut(s) 223
TasI AATT 4 cut(s) 66, 313, 428, 561
TfiI GAWTC 2 cut(s) 309, 335
Tru1I TTAA 2 cut(s) 431, 525
Tru9I TTAA 2 cut(s) 431, 525
TscAI CASTG 1 cut(s) 228
TseI GCWGC 1 cut(s) 138
TspDTI ATGAA 2 cut(s) 17, 593
TspRI CASTG 1 cut(s) 228
VpaK11BI GGWCC 1 cut(s) 470
XceI RCATGY 1 cut(s) 166
XmnI GAANNNNTTC 1 cut(s) 181
XspI CTAG 2 cut(s) 498, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.