Rmu_ssc0000320.1_g000001

ADP-ribosylation factor GTPase-activating protein AGD14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000320.1
OG0006003
Physical Location & Seq
Forward (+)
1 .. 2577
2577 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 986 bp
tccatagatctccgcaacgacgtcgtttgctagaaccgcaccggagcttcttcgaggaagtcaaaatggcgagccgagtgaaggaggacgaaaaaaatgaacggattattcgaggccttcttaaacttcctgagaatcgcagatgcattaactgcaatagtctggggccgcagtatgtttgcacgaatttctggacatttgtttgcaccaactgcagtggaatacatcgggagttcacgcaccgagtaaaatctgtgtcaatggccaaatttacgtcgcaagaagttaaatcccttcaagaagggggaaaccagcgtgcaaaggaactttatcaaaaagaattagatccacaacgtaattccttccctgatagcagtaatgttgagagacttcgagactttattaagcatgtgtacgtggatagaagatatactggtgaaagaaactcggacaagcctccgagggtgaaaatgggtgacaaggaagactcttatgaaaatagaaggccagattcatatcagggttctcgaagcccaccatatgatgatgaacgtcgttatagtggaaggtccagccccggtggtagaagttatgaggagcaaagaagtcctggatatgatctagaaagtcgacagtatggtgattctaagagaagtccttctcgccctgaaattgtcaatgattggcgccgagaagatagatttggaaatggtaggagatatgatgatcgtagagtatccgatggagattctaaactggacgcaaaatctcctgaacgaccaagagatatagattcgtccagtccccctatggtacgacctgttagagatattttgggggataagacaatacctctccgtattattgaacctcccaaagccaacaatgttaggcctactgaaggttccgtgcttacacaggtgatgagtctccactttaaaactgcatactgcatatatctgctgattgtaacctgctttatttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArfGap

Protein Analysis

327

Amino Acids

38.27

Weight (kDa)

9.48

Isoelectric Point (pI)

75.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 34 - 144 1.1e-30 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 24
Acc36I ACCTGC 1 cut(s) 982
AccB1I GGYRCC 1 cut(s) 686
AccI GTMKAC 1 cut(s) 630
AciI CCGC 3 cut(s) 13, 37, 169
AclWI GGATC 1 cut(s) 340
AcoI YGGCCR 1 cut(s) 263
AcsI RAATTY 2 cut(s) 186, 268
AcuI CTGAAG 1 cut(s) 920
AcyI GRCGYC 2 cut(s) 21, 687
AfaI GTAC 2 cut(s) 415, 815
AfiI CCNNNNNNNGG 2 cut(s) 81, 901
AgsI TTSAA 2 cut(s) 298, 868
AjnI CCWGG 1 cut(s) 609
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
AluBI AGCT 1 cut(s) 47
AluI AGCT 1 cut(s) 47
Alw26I GTCTC 3 cut(s) 381, 389, 934
AlwI GGATC 1 cut(s) 340
AoxI GGCC 5 cut(s) 114, 166, 263, 505, 892
ApoI RAATTY 2 cut(s) 186, 268
Asp700I GAANNNNTTC 1 cut(s) 657
AspLEI GCGC 1 cut(s) 689
AspS9I GGNCC 2 cut(s) 166, 569
AsuC2I CCSGG 1 cut(s) 578
AsuHPI GGTGA 5 cut(s) 448, 477, 487, 652, 933
AvaII GGWCC 1 cut(s) 569
BalI TGGCCA 1 cut(s) 265
BanI GGYRCC 1 cut(s) 686
BbsI GAAGAC 1 cut(s) 491
BccI CCATC 1 cut(s) 736
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciT130I CCWGG 1 cut(s) 611
BciVI GTATCC 1 cut(s) 747
BcnI CCSGG 1 cut(s) 578
BcoDI GTCTC 3 cut(s) 381, 389, 934
BfaI CTAG 2 cut(s) 31, 622
BfmI CTRYAG 1 cut(s) 213
BfoI RGCGCY 1 cut(s) 690
BfuAI ACCTGC 1 cut(s) 982
BfuI GTATCC 1 cut(s) 747
BglII AGATCT 1 cut(s) 7
BisI GCNGC 1 cut(s) 169
BlsI GCNGC 1 cut(s) 170
Bme1390I CCNGG 2 cut(s) 578, 611
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 2 cut(s) 166, 569
BmiI GGNNCC 3 cut(s) 167, 688, 906
BmrFI CCNGG 2 cut(s) 578, 611
BmsI GCATC 1 cut(s) 133
BpiI GAAGAC 1 cut(s) 491
BpuMI CCSGG 1 cut(s) 578
BsaAI YACGTR 1 cut(s) 417
BsaBI GATNNNNATC 1 cut(s) 515
BsaHI GRCGYC 2 cut(s) 21, 687
BsaJI CCNNGG 2 cut(s) 460, 576
BsaWI WCCGGW 1 cut(s) 41
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 901
Bse1I ACTGG 3 cut(s) 438, 761, 800
Bse8I GATNNNNATC 1 cut(s) 515
BseBI CCWGG 1 cut(s) 611
BseDI CCNNGG 2 cut(s) 460, 576
BseJI GATNNNNATC 1 cut(s) 515
BseLI CCNNNNNNNGG 2 cut(s) 81, 901
BseMII CTCAG 1 cut(s) 122
BseNI ACTGG 3 cut(s) 438, 761, 800
BseRI GAGGAG 1 cut(s) 610
BshFI GGCC 5 cut(s) 116, 168, 265, 507, 894
BshNI GGYRCC 1 cut(s) 686
BsiSI CCGG 2 cut(s) 42, 578
BslFI GGGAC 1 cut(s) 788
BslI CCNNNNNNNGG 2 cut(s) 81, 901
BsmAI GTCTC 3 cut(s) 381, 389, 934
BsmFI GGGAC 1 cut(s) 788
BsnI GGCC 5 cut(s) 116, 168, 265, 507, 894
Bsp143I GATC 4 cut(s) 7, 345, 618, 726
BspACI CCGC 3 cut(s) 13, 37, 169
BspANI GGCC 5 cut(s) 116, 168, 265, 507, 894
BspCNI CTCAG 1 cut(s) 123
BspLI GGNNCC 3 cut(s) 167, 688, 906
BspMAI CTGCAG 1 cut(s) 217
BspMI ACCTGC 1 cut(s) 982
BspPI GGATC 1 cut(s) 340
BspT107I GGYRCC 1 cut(s) 686
BsrI ACTGG 3 cut(s) 438, 761, 800
BssECI CCNNGG 2 cut(s) 460, 576
BssMI GATC 4 cut(s) 7, 345, 618, 726
BssNI GRCGYC 2 cut(s) 21, 687
Bst2UI CCWGG 1 cut(s) 611
Bst4CI ACNGT 1 cut(s) 635
BstACI GRCGYC 2 cut(s) 21, 687
BstAPI GCANNNNNTGC 2 cut(s) 152, 212
BstBAI YACGTR 1 cut(s) 417
BstC8I GCNNGC 2 cut(s) 72, 317
BstDEI CTNAG 2 cut(s) 131, 647
BstENI CCTNNNNNAGG 1 cut(s) 899
BstH2I RGCGCY 1 cut(s) 690
BstHHI GCGC 1 cut(s) 689
BstKTI GATC 4 cut(s) 10, 348, 621, 729
BstMAI GTCTC 3 cut(s) 381, 389, 934
BstMBI GATC 4 cut(s) 7, 345, 618, 726
BstMWI GCNNNNNNNGC 3 cut(s) 36, 152, 212
BstNI CCWGG 1 cut(s) 611
BstNSI RCATGY 1 cut(s) 412
BstSCI CCNGG 2 cut(s) 576, 609
BstSFI CTRYAG 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 491
BstX2I RGATCY 2 cut(s) 7, 345
BstYI RGATCY 2 cut(s) 7, 345
BsuI GTATCC 1 cut(s) 747
BsuRI GGCC 5 cut(s) 116, 168, 265, 507, 894
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 222
BveI ACCTGC 1 cut(s) 982
Cac8I GCNNGC 2 cut(s) 72, 317
CfoI GCGC 1 cut(s) 689
Cfr13I GGNCC 2 cut(s) 166, 569
CseI GACGC 1 cut(s) 769
Csp6I GTAC 2 cut(s) 414, 814
CspCI CAANNNNNGTGG 2 cut(s) 198, 233
CviAII CATG 1 cut(s) 409
CviQI GTAC 2 cut(s) 414, 814
DdeI CTNAG 2 cut(s) 131, 647
DinI GGCGCC 1 cut(s) 688
DpnI GATC 4 cut(s) 9, 347, 620, 728
DpnII GATC 4 cut(s) 7, 345, 618, 726
DraI TTTAAA 1 cut(s) 939
EaeI YGGCCR 1 cut(s) 263
Eco147I AGGCCT 2 cut(s) 116, 894
Eco47I GGWCC 1 cut(s) 569
Eco57I CTGAAG 1 cut(s) 920
EcoNI CCTNNNNNAGG 1 cut(s) 899
EcoRII CCWGG 1 cut(s) 609
EcoT22I ATGCAT 1 cut(s) 148
EgeI GGCGCC 1 cut(s) 688
EheI GGCGCC 1 cut(s) 688
FaeI CATG 1 cut(s) 412
FaqI GGGAC 1 cut(s) 788
FatI CATG 1 cut(s) 408
FauNDI CATATG 1 cut(s) 540
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 1 cut(s) 169
Fsp4HI GCNGC 1 cut(s) 169
FspBI CTAG 2 cut(s) 31, 622
GlaI GCGC 1 cut(s) 688
GluI GCNGC 1 cut(s) 169
HaeII RGCGCY 1 cut(s) 690
HaeIII GGCC 5 cut(s) 116, 168, 265, 507, 894
HapII CCGG 2 cut(s) 42, 578
HgaI GACGC 1 cut(s) 769
HhaI GCGC 1 cut(s) 689
Hin1I GRCGYC 2 cut(s) 21, 687
Hin1II CATG 1 cut(s) 412
Hin6I GCGC 1 cut(s) 687
HinP1I GCGC 1 cut(s) 687
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 7 cut(s) 135, 487, 511, 643, 748, 793, 927
HpaII CCGG 2 cut(s) 42, 578
HphI GGTGA 5 cut(s) 448, 477, 487, 652, 933
Hpy166II GTNNAC 3 cut(s) 236, 414, 631
Hpy188I TCNGA 3 cut(s) 450, 461, 741
Hpy188III TCNNGA 8 cut(s) 130, 192, 229, 298, 394, 527, 622, 772
Hpy8I GTNNAC 3 cut(s) 236, 414, 631
Hpy99I CGWCG 4 cut(s) 23, 26, 279, 557
HpyAV CCTTC 9 cut(s) 75, 127, 295, 304, 372, 497, 560, 668, 895
HpyCH4III ACNGT 1 cut(s) 635
HpyCH4IV ACGT 5 cut(s) 21, 274, 354, 416, 552
HpyCH4V TGCA 8 cut(s) 146, 155, 182, 206, 215, 319, 946, 953
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 152, 212
HpyF3I CTNAG 2 cut(s) 131, 647
HpySE526I ACGT 5 cut(s) 21, 274, 354, 416, 552
Hsp92I GRCGYC 2 cut(s) 21, 687
Hsp92II CATG 1 cut(s) 412
HspAI GCGC 1 cut(s) 687
KasI GGCGCC 1 cut(s) 686
Kzo9I GATC 4 cut(s) 7, 345, 618, 726
LmnI GCTCC 2 cut(s) 44, 597
LweI GCATC 1 cut(s) 133
MaeI CTAG 2 cut(s) 31, 622
MaeII ACGT 5 cut(s) 21, 274, 354, 416, 552
MaeIII GTNAC 2 cut(s) 475, 969
MalI GATC 4 cut(s) 9, 347, 620, 728
MboI GATC 4 cut(s) 7, 345, 618, 726
MboII GAAGA 4 cut(s) 42, 437, 496, 706
MflI RGATCY 2 cut(s) 7, 345
MlsI TGGCCA 1 cut(s) 265
MluCI AATT 5 cut(s) 186, 268, 340, 357, 671
MluNI TGGCCA 1 cut(s) 265
Mly113I GGCGCC 1 cut(s) 687
MlyI GAGTC 2 cut(s) 481, 936
MnlI CCTC 8 cut(s) 48, 78, 106, 455, 467, 588, 863, 881
Mox20I TGGCCA 1 cut(s) 265
Mph1103I ATGCAT 1 cut(s) 148
MroXI GAANNNNTTC 1 cut(s) 657
MscI TGGCCA 1 cut(s) 265
MseI TTAA 5 cut(s) 122, 149, 287, 404, 938
Msp20I TGGCCA 1 cut(s) 265
MspI CCGG 2 cut(s) 42, 578
MspR9I CCNGG 2 cut(s) 578, 611
MvaI CCWGG 1 cut(s) 611
MwoI GCNNNNNNNGC 3 cut(s) 36, 152, 212
NarI GGCGCC 1 cut(s) 687
NciI CCSGG 1 cut(s) 578
NdeI CATATG 1 cut(s) 540
NdeII GATC 4 cut(s) 7, 345, 618, 726
NlaIII CATG 1 cut(s) 412
NlaIV GGNNCC 3 cut(s) 167, 688, 906
NmeAIII GCCGAG 2 cut(s) 100, 715
NmuCI GTSAC 1 cut(s) 475
NsiI ATGCAT 1 cut(s) 148
NspI RCATGY 1 cut(s) 412
PceI AGGCCT 2 cut(s) 116, 894
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 657
PfeI GAWTC 5 cut(s) 135, 511, 643, 748, 793
PfoI TCCNGGA 1 cut(s) 609
PkrI GCNGC 1 cut(s) 170
PleI GAGTC 2 cut(s) 481, 935
PluTI GGCGCC 1 cut(s) 690
PpsI GAGTC 2 cut(s) 481, 935
Ppu21I YACGTR 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 609
PspGI CCWGG 1 cut(s) 609
PspN4I GGNNCC 3 cut(s) 167, 688, 906
PspPI GGNCC 2 cut(s) 166, 569
PstI CTGCAG 1 cut(s) 217
PsuI RGATCY 2 cut(s) 7, 345
RsaI GTAC 2 cut(s) 415, 815
RsaNI GTAC 2 cut(s) 414, 814
SalI GTCGAC 1 cut(s) 629
SaqAI TTAA 5 cut(s) 122, 149, 287, 404, 938
SatI GCNGC 1 cut(s) 169
Sau3AI GATC 4 cut(s) 7, 345, 618, 726
Sau96I GGNCC 2 cut(s) 166, 569
SchI GAGTC 2 cut(s) 481, 936
ScrFI CCNGG 2 cut(s) 578, 611
SfaNI GCATC 1 cut(s) 133
SfcI CTRYAG 1 cut(s) 213
SfoI GGCGCC 1 cut(s) 688
SinI GGWCC 1 cut(s) 569
Sse9I AATT 5 cut(s) 186, 268, 340, 357, 671
SseBI AGGCCT 2 cut(s) 116, 894
SsiI CCGC 3 cut(s) 13, 37, 169
SspDI GGCGCC 1 cut(s) 686
SspMI CTAG 2 cut(s) 31, 622
StuI AGGCCT 2 cut(s) 116, 894
StyD4I CCNGG 2 cut(s) 576, 609
TaaI ACNGT 1 cut(s) 635
TaiI ACGT 5 cut(s) 24, 277, 357, 419, 555
TaqI TCGA 5 cut(s) 53, 111, 393, 528, 630
TasI AATT 5 cut(s) 186, 268, 340, 357, 671
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 5 cut(s) 135, 511, 643, 748, 793
Tru1I TTAA 5 cut(s) 122, 149, 287, 404, 938
Tru9I TTAA 5 cut(s) 122, 149, 287, 404, 938
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 1 cut(s) 475
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 4 cut(s) 113, 503, 509, 563
TspGWI ACGGA 3 cut(s) 117, 847, 897
TspRI CASTG 1 cut(s) 222
VpaK11BI GGWCC 1 cut(s) 569
XagI CCTNNNNNAGG 1 cut(s) 899
XapI RAATTY 2 cut(s) 186, 268
XbaI TCTAGA 1 cut(s) 621
XceI RCATGY 1 cut(s) 412
XcmI CCANNNNNNNNNTGG 1 cut(s) 807
XmiI GTMKAC 1 cut(s) 630
XmnI GAANNNNTTC 1 cut(s) 657
XspI CTAG 2 cut(s) 31, 622
ZraI GACGTC 1 cut(s) 22
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.