Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_ssc0000373.1

Orthogroup ID

OG0008061

Physical Location & Seq

Genomic Coordinates Reverse (-)

9288 .. 23865

Gene Structure 14578 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3222 bp

atggaggacgcctacgccagatccgccaccgaggtgttggatttctttggcgtcgacccgaaacgaggtctcagtgacactcaggtttcgcaacatgctagactttatggccaaaacggggcttcgttctggaaacttgtcctgaaacagtttgatgatttgcttgttaagatattgattgttgcagcaatcgtttcttttgttctggctctaattaatggagatacaggcttaacagcatttttggagccttctgtaagtggggcttcgttctggaaacttgtcctgaaacagtttgatgatttgcttgttaagatattgattgttgcagcaatcgtttcttttgttctggctctaattaatggagatacaggcttaacagcatttttggagccttctgttatcctcacgatattggctgcaaatgctgcagtaggagtgattacagaaactaatgcagaaaaggctcttgaggagctacgcgcataccaagctgataacgcaaccgttctgcgcaatggttgcttctccatacttccagcaacagagctcgtccctggggatatcgttgaagtggctgtgggatgcaaaattccagctgatatgagaatgattgagatgctcagtaatcaattacgtgttgatcaggcaatccttacaggtttatctttacctattgaattgatgcttttgatatgtgtagtgggatgcaaaattccagccgatatgagaatgattgagatgctcagtaatcaattacgtgttgatcaggcaatccttacaggtgagagttgctctgtggagaaagaccttgaatccaccactgcaacaaatgctgtatatcaagacaagacaaatattcttttctcgggtacggtagtcgtggctggtagggctagagctgttgtagtaagagttggaactcagactgctatgggcggcatacgagactcaatgttgcaaacggaagatgaagtgacaccattgaaaaagaagctggatgaatttggtacctttttggccaaggttattgctggcatatgtgtactcgtatggattgtaaatataggtcactttcgtgaccctgctcatggtggattcttgcgtggtgcaattcattactttaagattgcagttgcacttgcagttgcagcaatccctgaaggccttcctgctgttgttacaacgttgaatgctattgtaaggtcattgccatcagttgagaccctaggctgcactacagtaatttgcagtgacaaaactgggactctgactaccaatatgatgtctgcctctaaggtttgtgtgcttcacactgtacagcacattcctgttatctcagaatacagtgtcagcggaactacatttgcacctgaaggcactatttttgacagtactggcaatcagcttgaatttccagctcaattgccttgtcttcttcacatagcaatggcttcagcactttgcaatgaatctgtcttgcaatataatccagacaaggggagctatgaaaaaattggcgagtcaactgaagtagccctccgtgtccttgcggagaagattggtcttcctggctatgattctatgccatcttctctgaatttgctaagcaagcatgaacgcgcatcttactgtaaccattattgggagaatcaacttaaaaagatttctgttgcagattttactcgtgatcgtaaaatgatgagtgtcctctgtagccgaagtcagttgcagattatgttctgcaaaggtgctccagagagcattatttctagatgcaccaatattctgtgcaatgatgatggctccactataccattaactgctaatatccgagctgagttggaatcaaggttccacagttttgcaggaaaagaaaccttaagatgcttggctcttgctttcaagcggatgccgatggatgtaccaactctctcccacaatgatgagaaggatcttacattcattgggttggtcggaatgcttgatccaccaagagaggaagtgaaaaatgctatgctttcatgcatgactgctggcatacgcgttatagttgtcaccggggataacaaatccacagccgaatccctttgccgcaagataggtgcttttgatcactttgtagatcttgctgggcactcttacaccgcaactgagtttgaagagttaccagctttgcagaaaacgatagcattgcaacggatggcattattcaccagggtcgagccttctcataaaaggatgctggttgaggccttacaacaccagaatgaagtggttgcaatgactggtgatggtgttaatgatgcacctgcactaaagaaggcagacattggaattgccatgggttcaggaacagctgttgccaagagtgcttcagatatggttttggctgatgacaattttgccactattgttgcggctgttgcagagggaagggcaatttacaataacacaaaacaattcatcagatatatgatctcttcaaatattggtgaagttgtttgtatatttgtggcagctgtacttggaatacctgatacactggcccctgtccagctgctctgggtcaacttagttactgatgggttgcctgccacagcaattggttttaataagcaagattctgatgtgatgaaggctaaacctcgtaaggttaatgaagcagttgttactggatggttgttttttcgttatttggtgattggagcttatgttggtcttgccacagttgctggatttatttggtggtttctttactctgatactggtcctaaactgccatatatggaactgataaattctgacacatgcggaatgagggagacgacttatccgtgcagtatatttagtgatcgccatccatcaactgtctccatgactgttcttgtggttgttgaaatgttcaatgctttgaataatctcagtgaaaaccaatcacttcttgttatccctccctggagcaacttatggcttgttggttcaatcatcataacgatgatccttcacgtactaatattatatgtgcatcccctctcagttcttttctctgtaacaccattatcttgggctgagtggactgttgtgctatacctttcgtttccggtcataatcattgatgaagtgctaaagttcttctcaagaaattccagtggtgtaaggttaaatttccagttaaggaggcgtgatcgactgccaagaaaggaattgcatgataagtag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0003824 GO:0005215 GO:0005384 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006828 GO:0007275 GO:0008150 GO:0008324 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015410 GO:0015662 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022622 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0034220 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055071 GO:0055076 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071421 GO:0072511 GO:0090662 GO:0097708 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0099131 GO:0099132 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04020 ko04022 ko04972 ko05010 map04020 map04022 map04972 map05010

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1073

Amino Acids

117.19

Weight (kDa)

5.73

Isoelectric Point (pI)

33.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	5 - 66	2.3e-12	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	164 - 227	2.2e-12	P-type ATPase actuator domain
E1-E2_ATPase	PF00122	235 - 318	2.2e-14	P-type ATPase actuator domain
Cation_ATPase	PF13246	481 - 668	7.8e-41	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	651 - 777	2.4e-14	haloacid dehalogenase-like hydrolase
Hydrolase_3	PF08282	749 - 804	3.3e-06	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	848 - 1046	3.1e-45	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0008061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10130
fragaria_vesca	FvH4_2g32870 FvH4_2g32870 FvH4_2g32870
malus_domestica	MD08G1080200.v1.1 MD15G1067300.v1.1
prunus_persica	Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1
pyrus_communis	pycom08g06620 pycom15g06380
rosa_chinensis	RchiOBHm_Chr6g0309841
rosa_laevigata	RLG00000010530
rosa_multiflora	Rmu_ssc0000373.1_g000002
rosa_roxburghii	Rroxscaffold_7G00159130
rosa_rugosa	Rorug06G0375200 Rorug06G0375300
rosa_samantha	Rh6AG488700 Rh6BG498200 Rh6CG503000 Rh6DG489200
rosa_wichuraiana	Rw6G042560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2320
Acc16I	TGCGCA	1 cut(s)	515
Acc36I	ACCTGC	1 cut(s)	2320
Acc65I	GGTACC	1 cut(s)	1010
AccB1I	GGYRCC	1 cut(s)	1010
AccI	GTMKAC	1 cut(s)	54
AccII	CGCG	3 cut(s)	483, 1623, 2046
AciI	CCGC	9 cut(s)	24, 939, 1356, 1553, 1909, 2095, 2148, 2420, 2814
AclI	AACGTT	1 cut(s)	1187
AclWI	GGATC	4 cut(s)	15, 1962, 1982, 2995
AcoI	YGGCCR	2 cut(s)	109, 1020
AcsI	RAATTY	8 cut(s)	591, 714, 1004, 1412, 1600, 2800, 3145, 3166
AcuI	CTGAAG	5 cut(s)	1182, 1395, 1440, 1551, 2361
AcyI	GRCGYC	2 cut(s)	9, 51
AfaI	GTAC	8 cut(s)	874, 1012, 1047, 1320, 1396, 1926, 2526, 3012
AfiI	CCNNNNNNNGG	5 cut(s)	65, 118, 1091, 1499, 1645
AflII	CTTAAG	1 cut(s)	1882
AflIII	ACRYGT	3 cut(s)	637, 760, 2044
AjnI	CCWGG	4 cut(s)	556, 1570, 2216, 2957
AleI	CACNNNNGTG	2 cut(s)	32, 1077
AloI	GAACNNNNNNTCC	2 cut(s)	1838, 1870
Alw21I	GWGCWC	2 cut(s)	552, 1756
Alw26I	GTCTC	5 cut(s)	74, 942, 1217, 2819, 2878
AlwI	GGATC	4 cut(s)	15, 1962, 1982, 2995
AlwNI	CAGNNNCTG	1 cut(s)	2735
Ama87I	CYCGRG	1 cut(s)	868
AoxI	GGCC	5 cut(s)	109, 1020, 1165, 2253, 2547
ApeKI	GCWGC	8 cut(s)	185, 329, 419, 428, 1151, 1233, 2519, 2560
ApoI	RAATTY	8 cut(s)	591, 714, 1004, 1412, 1600, 2800, 3145, 3166
AseI	ATTAAT	2 cut(s)	216, 360
Asp700I	GAANNNNTTC	1 cut(s)	1167
Asp718I	GGTACC	1 cut(s)	1010
AspA2I	CCTAGG	1 cut(s)	1228
AspLEI	GCGC	3 cut(s)	485, 516, 1625
AspS9I	GGNCC	2 cut(s)	2548, 2771
AsuC2I	CCSGG	1 cut(s)	2062
AsuHPI	GGTGA	6 cut(s)	797, 2050, 2206, 2303, 2507, 2713
AvaI	CYCGRG	1 cut(s)	868
AvaII	GGWCC	1 cut(s)	2771
AvrII	CCTAGG	1 cut(s)	1228
BaeGI	GKGCMC	1 cut(s)	2139
BaeI	ACNNNNGTAYC	2 cut(s)	864, 897
BalI	TGGCCA	2 cut(s)	111, 1022
BanI	GGYRCC	1 cut(s)	1010
BanII	GRGCYC	1 cut(s)	552
BauI	CACGAG	1 cut(s)	1686
BbsI	GAAGAC	2 cut(s)	1427, 1559
Bbv12I	GWGCWC	2 cut(s)	552, 1756
BbvI	GCAGC	8 cut(s)	197, 341, 406, 415, 1163, 1220, 2531, 2547
BcgI	CGANNNNNNTGC	2 cut(s)	2176, 2210
BciT130I	CCWGG	4 cut(s)	558, 1572, 2218, 2959
BclI	TGATCA	3 cut(s)	643, 766, 2113
BcnI	CCSGG	1 cut(s)	2062
BcoDI	GTCTC	5 cut(s)	74, 942, 1217, 2819, 2878
BfaI	CTAG	4 cut(s)	99, 897, 1229, 1773
BfmI	CTRYAG	3 cut(s)	429, 1239, 1714
BfrI	CTTAAG	1 cut(s)	1882
BfuAI	ACCTGC	1 cut(s)	2320
BglII	AGATCT	1 cut(s)	2125
BlnI	CCTAGG	1 cut(s)	1228
BlpI	GCTNAGC	1 cut(s)	1607
BmcAI	AGTACT	1 cut(s)	1396
Bme1390I	CCNGG	5 cut(s)	558, 1572, 2062, 2218, 2959
Bme18I	GGWCC	1 cut(s)	2771
BmeT110I	CYCGRG	1 cut(s)	868
BmgT120I	GGNCC	2 cut(s)	2548, 2771
BmiI	GGNNCC	6 cut(s)	249, 393, 1012, 1807, 1856, 2550
BmrFI	CCNGG	5 cut(s)	558, 1572, 2062, 2218, 2959
BmrI	ACTGGG	1 cut(s)	1272
BmuI	ACTGGG	1 cut(s)	1272
BpiI	GAAGAC	2 cut(s)	1427, 1559
BplI	GAGNNNNNCTC	2 cut(s)	779, 811
BpmI	CTGGAG	2 cut(s)	1740, 2980
Bpu1102I	GCTNAGC	1 cut(s)	1607
BpuEI	CTTGAG	2 cut(s)	491, 3124
BpuMI	CCSGG	1 cut(s)	2062
BsaAI	YACGTR	3 cut(s)	638, 761, 3010
BsaBI	GATNNNNATC	1 cut(s)	2859
BsaHI	GRCGYC	2 cut(s)	9, 51
BsaI	GGTCTC	2 cut(s)	74, 1217
BsaJI	CCNNGG	9 cut(s)	30, 556, 557, 1023, 1228, 2061, 2217, 2343, 2957
BsaWI	WCCGGW	1 cut(s)	3103
BsaXI	ACNNNNNCTCC	2 cut(s)	2700, 2730
Bsc4I	CCNNNNNNNGG	5 cut(s)	65, 118, 1091, 1499, 1645
Bse1I	ACTGG	8 cut(s)	1267, 1402, 2293, 2550, 2680, 2773, 3150, 3172
Bse3DI	GCAATG	7 cut(s)	523, 1208, 1455, 1474, 1801, 2192, 2289
Bse8I	GATNNNNATC	1 cut(s)	2859
BseBI	CCWGG	4 cut(s)	558, 1572, 2218, 2959
BseDI	CCNNGG	9 cut(s)	30, 556, 557, 1023, 1228, 2061, 2217, 2343, 2957
BseJI	GATNNNNATC	1 cut(s)	2859
BseLI	CCNNNNNNNGG	5 cut(s)	65, 118, 1091, 1499, 1645
BseMI	GCAATG	7 cut(s)	523, 1208, 1455, 1474, 1801, 2192, 2289
BseNI	ACTGG	8 cut(s)	1267, 1402, 2293, 2550, 2680, 2773, 3150, 3172
BseRI	GAGGAG	1 cut(s)	488
BseSI	GKGCMC	1 cut(s)	2139
BseXI	GCAGC	8 cut(s)	197, 341, 406, 415, 1163, 1220, 2531, 2547
BseYI	CCCAGC	1 cut(s)	2132
BsgI	GTGCAG	3 cut(s)	1219, 2298, 2860
Bsh1236I	CGCG	3 cut(s)	483, 1623, 2046
BshFI	GGCC	5 cut(s)	111, 1022, 1167, 2255, 2549
BshNI	GGYRCC	1 cut(s)	1010
BsiHKAI	GWGCWC	2 cut(s)	552, 1756
BsiHKCI	CYCGRG	1 cut(s)	868
BsiSI	CCGG	2 cut(s)	2061, 3104
BslFI	GGGAC	2 cut(s)	539, 1279
BslI	CCNNNNNNNGG	5 cut(s)	65, 118, 1091, 1499, 1645
BsmAI	GTCTC	5 cut(s)	74, 942, 1217, 2819, 2878
BsmBI	CGTCTC	1 cut(s)	2819
BsmFI	GGGAC	2 cut(s)	539, 1279
BsmI	GAATGC	2 cut(s)	1198, 1986
BsnI	GGCC	5 cut(s)	111, 1022, 1167, 2255, 2549
Bso31I	GGTCTC	2 cut(s)	74, 1217
BsoBI	CYCGRG	1 cut(s)	868
Bsp1286I	GDGCHC	3 cut(s)	552, 1756, 2139
Bsp1407I	TGTACA	1 cut(s)	1318
Bsp1720I	GCTNAGC	1 cut(s)	1607
Bsp19I	CCATGG	1 cut(s)	2343
BspACI	CCGC	9 cut(s)	24, 939, 1356, 1553, 1909, 2095, 2148, 2420, 2814
BspANI	GGCC	5 cut(s)	111, 1022, 1167, 2255, 2549
BspFNI	CGCG	3 cut(s)	483, 1623, 2046
BspLI	GGNNCC	6 cut(s)	249, 393, 1012, 1807, 1856, 2550
BspMAI	CTGCAG	1 cut(s)	433
BspMI	ACCTGC	1 cut(s)	2320
BspPI	GGATC	4 cut(s)	15, 1962, 1982, 2995
BspT107I	GGYRCC	1 cut(s)	1010
BspTI	CTTAAG	1 cut(s)	1882
BspTNI	GGTCTC	2 cut(s)	74, 1217
BsrDI	GCAATG	7 cut(s)	523, 1208, 1455, 1474, 1801, 2192, 2289
BsrGI	TGTACA	1 cut(s)	1318
BsrI	ACTGG	8 cut(s)	1267, 1402, 2293, 2550, 2680, 2773, 3150, 3172
BssECI	CCNNGG	9 cut(s)	30, 556, 557, 1023, 1228, 2061, 2217, 2343, 2957
BssNI	GRCGYC	2 cut(s)	9, 51
BssSI	CACGAG	1 cut(s)	1686
BssT1I	CCWWGG	3 cut(s)	1023, 1228, 2343
Bst2BI	CACGAG	1 cut(s)	1686
Bst2UI	CCWGG	4 cut(s)	558, 1572, 2218, 2959
Bst6I	CTCTTC	2 cut(s)	2157, 2488
BstACI	GRCGYC	2 cut(s)	9, 51
BstAFI	CTTAAG	1 cut(s)	1882
BstAPI	GCANNNNNTGC	3 cut(s)	428, 522, 833
BstAUI	TGTACA	1 cut(s)	1318
BstBAI	YACGTR	3 cut(s)	638, 761, 3010
BstC8I	GCNNGC	4 cut(s)	1036, 1613, 2038, 2595
BstDSI	CCRYGG	1 cut(s)	2343
BstFNI	CGCG	3 cut(s)	483, 1623, 2046
BstHHI	GCGC	3 cut(s)	485, 516, 1625
BstMAI	GTCTC	5 cut(s)	74, 942, 1217, 2819, 2878
BstNI	CCWGG	4 cut(s)	558, 1572, 2218, 2959
BstNSI	RCATGY	2 cut(s)	98, 2814
BstSCI	CCNGG	5 cut(s)	556, 1570, 2060, 2216, 2957
BstSFI	CTRYAG	3 cut(s)	429, 1239, 1714
BstSLI	GKGCMC	1 cut(s)	2139
BstUI	CGCG	3 cut(s)	483, 1623, 2046
BstV1I	GCAGC	8 cut(s)	197, 341, 406, 415, 1163, 1220, 2531, 2547
BstV2I	GAAGAC	2 cut(s)	1427, 1559
BstX2I	RGATCY	3 cut(s)	20, 1954, 2125
BstXI	CCANNNNNNTGG	1 cut(s)	3066
BstYI	RGATCY	3 cut(s)	20, 1954, 2125
BsuRI	GGCC	5 cut(s)	111, 1022, 1167, 2255, 2549
BtgI	CCRYGG	1 cut(s)	2343
BtsI	GCAGTG	2 cut(s)	822, 1258
BtsIMutI	CAGTG	8 cut(s)	79, 822, 1258, 1314, 1354, 2543, 2932, 3157
BveI	ACCTGC	1 cut(s)	2320
Cac8I	GCNNGC	4 cut(s)	1036, 1613, 2038, 2595
CaiI	CAGNNNCTG	1 cut(s)	2735
CfoI	GCGC	3 cut(s)	485, 516, 1625
Cfr13I	GGNCC	2 cut(s)	2548, 2771
CseI	GACGC	2 cut(s)	17, 40
Csp6I	GTAC	8 cut(s)	873, 1011, 1046, 1319, 1395, 1925, 2525, 3011
CspCI	CAANNNNNGTGG	4 cut(s)	2398, 2433, 2587, 2622
CviAII	CATG	9 cut(s)	95, 1091, 1616, 2025, 2029, 2344, 2811, 2878, 3212
CviQI	GTAC	8 cut(s)	873, 1011, 1046, 1319, 1395, 1925, 2525, 3011
EaeI	YGGCCR	2 cut(s)	109, 1020
Eam1104I	CTCTTC	2 cut(s)	2157, 2488
EarI	CTCTTC	2 cut(s)	2157, 2488
EciI	GGCGGA	1 cut(s)	13
Ecl136II	GAGCTC	1 cut(s)	550
Eco130I	CCWWGG	3 cut(s)	1023, 1228, 2343
Eco147I	AGGCCT	2 cut(s)	1167, 2255
Eco24I	GRGCYC	1 cut(s)	552
Eco31I	GGTCTC	2 cut(s)	74, 1217
Eco32I	GATATC	1 cut(s)	565
Eco47I	GGWCC	1 cut(s)	2771
Eco53kI	GAGCTC	1 cut(s)	550
Eco57I	CTGAAG	5 cut(s)	1182, 1395, 1440, 1551, 2361
Eco88I	CYCGRG	1 cut(s)	868
EcoICRI	GAGCTC	1 cut(s)	550
EcoRII	CCWGG	4 cut(s)	556, 1570, 2216, 2957
EcoRV	GATATC	1 cut(s)	565
EcoT14I	CCWWGG	3 cut(s)	1023, 1228, 2343
EcoT22I	ATGCAT	1 cut(s)	2030
EcoT38I	GRGCYC	1 cut(s)	552
ErhI	CCWWGG	3 cut(s)	1023, 1228, 2343
Esp3I	CGTCTC	1 cut(s)	2819
FaeI	CATG	9 cut(s)	98, 1094, 1619, 2028, 2032, 2347, 2814, 2881, 3215
FalI	AAGNNNNNCTT	2 cut(s)	250, 282
FaqI	GGGAC	2 cut(s)	539, 1279
FatI	CATG	9 cut(s)	94, 1090, 1615, 2024, 2028, 2343, 2810, 2877, 3211
FauNDI	CATATG	1 cut(s)	1040
FbaI	TGATCA	3 cut(s)	643, 766, 2113
FblI	GTMKAC	1 cut(s)	54
FriOI	GRGCYC	1 cut(s)	552
FspBI	CTAG	4 cut(s)	99, 897, 1229, 1773
FspI	TGCGCA	1 cut(s)	515
GlaI	GCGC	3 cut(s)	484, 515, 1624
GsaI	CCCAGC	1 cut(s)	2136
GsuI	CTGGAG	2 cut(s)	1740, 2980
HaeIII	GGCC	5 cut(s)	111, 1022, 1167, 2255, 2549
HapII	CCGG	2 cut(s)	2061, 3104
HgaI	GACGC	2 cut(s)	17, 40
HhaI	GCGC	3 cut(s)	485, 516, 1625
Hin1I	GRCGYC	2 cut(s)	9, 51
Hin1II	CATG	9 cut(s)	98, 1094, 1619, 2028, 2032, 2347, 2814, 2881, 3215
Hin6I	GCGC	3 cut(s)	483, 514, 1623
HinP1I	GCGC	3 cut(s)	483, 514, 1623
HincII	GTYRAC	3 cut(s)	55, 1527, 2572
HindII	GTYRAC	3 cut(s)	55, 1527, 2572
HpaII	CCGG	2 cut(s)	2061, 3104
HphI	GGTGA	6 cut(s)	797, 2050, 2206, 2303, 2507, 2713
Hpy166II	GTNNAC	5 cut(s)	55, 1046, 1527, 2572, 3078
Hpy8I	GTNNAC	5 cut(s)	55, 1046, 1527, 2572, 3078
Hpy99I	CGWCG	1 cut(s)	56
HpyCH4IV	ACGT	4 cut(s)	637, 760, 1187, 3009
HpySE526I	ACGT	4 cut(s)	637, 760, 1187, 3009
Hsp92I	GRCGYC	2 cut(s)	9, 51
Hsp92II	CATG	9 cut(s)	98, 1094, 1619, 2028, 2032, 2347, 2814, 2881, 3215
HspAI	GCGC	3 cut(s)	483, 514, 1623
KpnI	GGTACC	1 cut(s)	1014
Ksp22I	TGATCA	3 cut(s)	643, 766, 2113
LmnI	GCTCC	8 cut(s)	247, 391, 475, 1503, 1759, 1811, 2708, 2961
Lsp1109I	GCAGC	8 cut(s)	197, 341, 406, 415, 1163, 1220, 2531, 2547
MaeI	CTAG	4 cut(s)	99, 897, 1229, 1773
MaeII	ACGT	4 cut(s)	637, 760, 1187, 3009
MboII	GAAGA	9 cut(s)	980, 1427, 1430, 1559, 1570, 1584, 2174, 2475, 3127
MfeI	CAATTG	2 cut(s)	1424, 2604
MflI	RGATCY	3 cut(s)	20, 1954, 2125
MhlI	GDGCHC	3 cut(s)	552, 1756, 2139
MlsI	TGGCCA	2 cut(s)	111, 1022
MluI	ACGCGT	1 cut(s)	2044
MluNI	TGGCCA	2 cut(s)	111, 1022
MlyI	GAGTC	3 cut(s)	944, 1261, 1532
MmeI	TCCRAC	4 cut(s)	18, 898, 1824, 1957
Mox20I	TGGCCA	2 cut(s)	111, 1022
Mph1103I	ATGCAT	1 cut(s)	2030
MroXI	GAANNNNTTC	1 cut(s)	1167
MscI	TGGCCA	2 cut(s)	111, 1022
MslI	CAYNNNNRTG	6 cut(s)	32, 1077, 1448, 1944, 2268, 2996
Msp20I	TGGCCA	2 cut(s)	111, 1022
MspA1I	CMGCKG	5 cut(s)	599, 1356, 2360, 2522, 2560
MspCI	CTTAAG	1 cut(s)	1882
MspI	CCGG	2 cut(s)	2061, 3104
MspR9I	CCNGG	5 cut(s)	558, 1572, 2062, 2218, 2959
MunI	CAATTG	2 cut(s)	1424, 2604
Mva1269I	GAATGC	2 cut(s)	1198, 1986
MvaI	CCWGG	4 cut(s)	558, 1572, 2218, 2959
MvnI	CGCG	3 cut(s)	483, 1623, 2046
NciI	CCSGG	1 cut(s)	2062
NcoI	CCATGG	1 cut(s)	2343
NdeI	CATATG	1 cut(s)	1040
NlaIII	CATG	9 cut(s)	98, 1094, 1619, 2028, 2032, 2347, 2814, 2881, 3215
NlaIV	GGNNCC	6 cut(s)	249, 393, 1012, 1807, 1856, 2550
NmuCI	GTSAC	6 cut(s)	74, 976, 1070, 1079, 1253, 2056
NsbI	TGCGCA	1 cut(s)	515
NsiI	ATGCAT	1 cut(s)	2030
NspI	RCATGY	2 cut(s)	98, 2814
OliI	CACNNNNGTG	2 cut(s)	32, 1077
PaqCI	CACCTGC	1 cut(s)	2320
PasI	CCCWGGG	1 cut(s)	557
PceI	AGGCCT	2 cut(s)	1167, 2255
PcsI	WCGNNNNNNNCGW	1 cut(s)	2834
PctI	GAATGC	2 cut(s)	1198, 1986
PdmI	GAANNNNTTC	1 cut(s)	1167
PfeI	GAWTC	8 cut(s)	815, 1098, 1472, 1580, 1651, 1847, 2084, 2624
PleI	GAGTC	3 cut(s)	944, 1261, 1531
PpsI	GAGTC	3 cut(s)	944, 1261, 1531
Ppu21I	YACGTR	3 cut(s)	638, 761, 3010
PshBI	ATTAAT	2 cut(s)	216, 360
Psp124BI	GAGCTC	1 cut(s)	552
Psp1406I	AACGTT	1 cut(s)	1187
Psp6I	CCWGG	4 cut(s)	556, 1570, 2216, 2957
PspFI	CCCAGC	1 cut(s)	2132
PspGI	CCWGG	4 cut(s)	556, 1570, 2216, 2957
PspN4I	GGNNCC	6 cut(s)	249, 393, 1012, 1807, 1856, 2550
PspPI	GGNCC	2 cut(s)	2548, 2771
PstI	CTGCAG	1 cut(s)	433
PstNI	CAGNNNCTG	1 cut(s)	2735
PsuI	RGATCY	3 cut(s)	20, 1954, 2125
PvuII	CAGCTG	4 cut(s)	599, 2360, 2522, 2560
RsaI	GTAC	8 cut(s)	874, 1012, 1047, 1320, 1396, 1926, 2526, 3012
RsaNI	GTAC	8 cut(s)	873, 1011, 1046, 1319, 1395, 1925, 2525, 3011
RseI	CAYNNNNRTG	6 cut(s)	32, 1077, 1448, 1944, 2268, 2996
SacI	GAGCTC	1 cut(s)	552
SalI	GTCGAC	1 cut(s)	53
Sau96I	GGNCC	2 cut(s)	2548, 2771
ScaI	AGTACT	1 cut(s)	1396
SchI	GAGTC	3 cut(s)	944, 1261, 1532
ScrFI	CCNGG	5 cut(s)	558, 1572, 2062, 2218, 2959
SduI	GDGCHC	3 cut(s)	552, 1756, 2139
SfcI	CTRYAG	3 cut(s)	429, 1239, 1714
SinI	GGWCC	1 cut(s)	2771
SmiMI	CAYNNNNRTG	6 cut(s)	32, 1077, 1448, 1944, 2268, 2996
SmlI	CTYRAG	3 cut(s)	470, 1882, 3139
SmoI	CTYRAG	3 cut(s)	470, 1882, 3139
SseBI	AGGCCT	2 cut(s)	1167, 2255
SsiI	CCGC	9 cut(s)	24, 939, 1356, 1553, 1909, 2095, 2148, 2420, 2814
SspI	AATATT	4 cut(s)	859, 1786, 2491, 3018
SspMI	CTAG	4 cut(s)	99, 897, 1229, 1773
SstI	GAGCTC	1 cut(s)	552
StuI	AGGCCT	2 cut(s)	1167, 2255
StyD4I	CCNGG	5 cut(s)	556, 1570, 2060, 2216, 2957
StyI	CCWWGG	3 cut(s)	1023, 1228, 2343
TaiI	ACGT	4 cut(s)	640, 763, 1190, 3012
TaqI	TCGA	3 cut(s)	54, 2223, 3190
TatI	WGTACW	4 cut(s)	1045, 1318, 1394, 2524
TauI	GCSGC	3 cut(s)	942, 2097, 2423
TfiI	GAWTC	8 cut(s)	815, 1098, 1472, 1580, 1651, 1847, 2084, 2624
TscAI	CASTG	8 cut(s)	79, 829, 1258, 1321, 1354, 2550, 2932, 3157
TseFI	GTSAC	6 cut(s)	74, 976, 1070, 1079, 1253, 2056
TseI	GCWGC	8 cut(s)	185, 329, 419, 428, 1151, 1233, 2519, 2560
Tsp45I	GTSAC	6 cut(s)	74, 976, 1070, 1079, 1253, 2056
TspGWI	ACGGA	4 cut(s)	980, 1532, 2215, 2826
TspRI	CASTG	8 cut(s)	79, 829, 1258, 1321, 1354, 2550, 2932, 3157
Vha464I	CTTAAG	1 cut(s)	1882
VpaK11BI	GGWCC	1 cut(s)	2771
VspI	ATTAAT	2 cut(s)	216, 360
XapI	RAATTY	8 cut(s)	591, 714, 1004, 1412, 1600, 2800, 3145, 3166
XbaI	TCTAGA	1 cut(s)	1772
XceI	RCATGY	2 cut(s)	98, 2814
XcmI	CCANNNNNNNNNTGG	1 cut(s)	34
XmaJI	CCTAGG	1 cut(s)	1228
XmiI	GTMKAC	1 cut(s)	54
XmnI	GAANNNNTTC	1 cut(s)	1167
XspI	CTAG	4 cut(s)	99, 897, 1229, 1773
ZrmI	AGTACT	1 cut(s)	1396
Zsp2I	ATGCAT	1 cut(s)	2030

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.