Rmu_ssc0000381.1_g000035

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000381.1
OG0009705
Physical Location & Seq
Forward (+)
167381 .. 167989
609 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 609 bp
atggccatgagaatagccagtgactattgggaagtggtgggcgacgctctctgtgacgtatatgtcaacccagattttgtggacgtcgtttctgaaccagaaaacgaggtgctatgcaccaccaagttcttgatcgatatccaggcggtgatcagacgcgtcacctcctgcgatgccgaacgactctcagacttggacgacgtcatcagccgcacgagtgtgactgaggcctcaattgaactctactcttctaactcagacgaagaagctgcagtctccaacgcactccgagagctgaaggtaccactgaatgaacgtgtccccatggtgcagagaataatgtacaaagcctgcgagatagaaaatgagaataggtgttatggaagcagaaagattgctcatatgtcggtgaagatcgatcttattgttgttgatgatgatggtggcgacgcagatgaggatggtattgtggagtgctgtcttgatggatcacatccttacccggatccttccatggtagagcagctacagaaaactggggtttgccctatgtgtgatgatgataggaaagtgtgcttgacaaagatggagaatgaggggattctgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

202

Amino Acids

22.54

Weight (kDa)

4.24

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
AatII GACGTC 2 cut(s) 87, 204
Acc65I GGTACC 1 cut(s) 301
AccB1I GGYRCC 1 cut(s) 301
AccII CGCG 1 cut(s) 159
AciI CCGC 2 cut(s) 146, 211
AclWI GGATC 3 cut(s) 496, 500, 513
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 317
AcyI GRCGYC 2 cut(s) 84, 201
AfaI GTAC 2 cut(s) 303, 344
AflIII ACRYGT 2 cut(s) 157, 316
AgsI TTSAA 1 cut(s) 239
AjnI CCWGG 1 cut(s) 141
AleI CACNNNNGTG 1 cut(s) 218
AluBI AGCT 3 cut(s) 269, 295, 526
AluI AGCT 3 cut(s) 269, 295, 526
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 3 cut(s) 496, 500, 513
AoxI GGCC 2 cut(s) 3, 228
ApeKI GCWGC 2 cut(s) 269, 523
Asp718I GGTACC 1 cut(s) 301
AsuC2I CCSGG 1 cut(s) 503
AsuHPI GGTGA 3 cut(s) 154, 160, 421
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 301
BauI CACGAG 1 cut(s) 214
BbvI GCAGC 2 cut(s) 256, 535
BccI CCATC 4 cut(s) 434, 455, 479, 580
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 1 cut(s) 143
BclI TGATCA 1 cut(s) 150
BcnI CCSGG 1 cut(s) 503
BcoDI GTCTC 1 cut(s) 280
BfmI CTRYAG 2 cut(s) 270, 527
BisI GCNGC 3 cut(s) 211, 270, 524
BlsI GCNGC 3 cut(s) 212, 271, 525
Bme1390I CCNGG 2 cut(s) 143, 503
BmiI GGNNCC 2 cut(s) 303, 507
BmrFI CCNGG 2 cut(s) 143, 503
BmrI ACTGGG 1 cut(s) 546
BmsI GCATC 1 cut(s) 163
BmuI ACTGGG 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 503
Bsa29I ATCGAT 2 cut(s) 135, 417
BsaBI GATNNNNATC 1 cut(s) 137
BsaHI GRCGYC 2 cut(s) 84, 201
BsaJI CCNNGG 2 cut(s) 324, 513
Bse1I ACTGG 2 cut(s) 18, 541
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 1 cut(s) 143
BseCI ATCGAT 2 cut(s) 135, 417
BseDI CCNNGG 2 cut(s) 324, 513
BseGI GGATG 2 cut(s) 466, 493
BseJI GATNNNNATC 1 cut(s) 137
BseMII CTCAG 3 cut(s) 201, 216, 270
BseNI ACTGG 2 cut(s) 18, 541
BseXI GCAGC 2 cut(s) 256, 535
BsgI GTGCAG 1 cut(s) 350
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 2 cut(s) 5, 230
BshNI GGYRCC 1 cut(s) 301
BshVI ATCGAT 2 cut(s) 135, 417
BsiSI CCGG 1 cut(s) 503
BslFI GGGAC 1 cut(s) 305
BsmAI GTCTC 1 cut(s) 280
BsmFI GGGAC 1 cut(s) 305
BsnI GGCC 2 cut(s) 5, 230
Bsp1407I TGTACA 1 cut(s) 342
Bsp143I GATC 6 cut(s) 132, 150, 414, 418, 488, 505
Bsp19I CCATGG 2 cut(s) 324, 513
BspACI CCGC 2 cut(s) 146, 211
BspANI GGCC 2 cut(s) 5, 230
BspCNI CTCAG 3 cut(s) 200, 217, 269
BspDI ATCGAT 2 cut(s) 135, 417
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 2 cut(s) 303, 507
BspMAI CTGCAG 1 cut(s) 274
BspPI GGATC 3 cut(s) 496, 500, 513
BspT107I GGYRCC 1 cut(s) 301
BsrGI TGTACA 1 cut(s) 342
BsrI ACTGG 2 cut(s) 18, 541
BssECI CCNNGG 2 cut(s) 324, 513
BssMI GATC 6 cut(s) 132, 150, 414, 418, 488, 505
BssNI GRCGYC 2 cut(s) 84, 201
BssSI CACGAG 1 cut(s) 214
BssT1I CCWWGG 2 cut(s) 324, 513
Bst2BI CACGAG 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 253
BstACI GRCGYC 2 cut(s) 84, 201
BstAUI TGTACA 1 cut(s) 342
BstC8I GCNNGC 1 cut(s) 352
BstDEI CTNAG 3 cut(s) 187, 225, 256
BstDSI CCRYGG 2 cut(s) 324, 513
BstF5I GGATG 2 cut(s) 466, 493
BstFNI CGCG 1 cut(s) 159
BstKTI GATC 6 cut(s) 135, 153, 417, 421, 491, 508
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 6 cut(s) 132, 150, 414, 418, 488, 505
BstNI CCWGG 1 cut(s) 143
BstSCI CCNGG 2 cut(s) 141, 501
BstSFI CTRYAG 2 cut(s) 270, 527
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 2 cut(s) 256, 535
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
Bsu15I ATCGAT 2 cut(s) 135, 417
BsuRI GGCC 2 cut(s) 5, 230
BsuTUI ATCGAT 2 cut(s) 135, 417
BtgI CCRYGG 2 cut(s) 324, 513
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 466, 493
BtsIMutI CAGTG 2 cut(s) 25, 305
Cac8I GCNNGC 1 cut(s) 352
ClaI ATCGAT 2 cut(s) 135, 417
CseI GACGC 4 cut(s) 53, 148, 165, 458
Csp6I GTAC 2 cut(s) 302, 343
CviAII CATG 3 cut(s) 7, 325, 514
CviJI RGCY 8 cut(s) 5, 17, 210, 230, 269, 295, 350, 526
CviKI_1 RGCY 8 cut(s) 5, 17, 210, 230, 269, 295, 350, 526
CviQI GTAC 2 cut(s) 302, 343
DdeI CTNAG 3 cut(s) 187, 225, 256
DpnI GATC 6 cut(s) 134, 152, 416, 420, 490, 507
DpnII GATC 6 cut(s) 132, 150, 414, 418, 488, 505
DrdI GACNNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 62
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 324, 513
Eco147I AGGCCT 1 cut(s) 230
Eco32I GATATC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 141
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 2 cut(s) 324, 513
ErhI CCWWGG 2 cut(s) 324, 513
FaeI CATG 3 cut(s) 10, 328, 517
FaqI GGGAC 1 cut(s) 305
FatI CATG 3 cut(s) 6, 324, 513
FauNDI CATATG 1 cut(s) 402
FbaI TGATCA 1 cut(s) 150
Fnu4HI GCNGC 3 cut(s) 211, 270, 524
FokI GGATG 2 cut(s) 473, 480
Fsp4HI GCNGC 3 cut(s) 211, 270, 524
GluI GCNGC 3 cut(s) 211, 270, 524
HaeIII GGCC 2 cut(s) 5, 230
HapII CCGG 1 cut(s) 503
HgaI GACGC 4 cut(s) 53, 148, 165, 458
Hin1I GRCGYC 2 cut(s) 84, 201
Hin1II CATG 3 cut(s) 10, 328, 517
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 2 cut(s) 183, 601
HpaII CCGG 1 cut(s) 503
HphI GGTGA 3 cut(s) 154, 160, 421
Hpy166II GTNNAC 2 cut(s) 67, 82
Hpy188I TCNGA 5 cut(s) 94, 155, 190, 259, 290
Hpy188III TCNNGA 2 cut(s) 130, 482
Hpy8I GTNNAC 2 cut(s) 67, 82
Hpy99I CGWCG 4 cut(s) 47, 89, 203, 452
HpyAV CCTTC 2 cut(s) 292, 519
HpyCH4IV ACGT 4 cut(s) 57, 84, 201, 316
HpyCH4V TGCA 3 cut(s) 117, 272, 331
HpyF3I CTNAG 3 cut(s) 187, 225, 256
HpySE526I ACGT 4 cut(s) 57, 84, 201, 316
Hsp92I GRCGYC 2 cut(s) 84, 201
Hsp92II CATG 3 cut(s) 10, 328, 517
KpnI GGTACC 1 cut(s) 305
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 6 cut(s) 132, 150, 414, 418, 488, 505
LpnPI CCDG 9 cut(s) 31, 84, 111, 128, 155, 181, 364, 516, 522
Lsp1109I GCAGC 2 cut(s) 256, 535
LweI GCATC 1 cut(s) 163
MaeII ACGT 4 cut(s) 57, 84, 201, 316
MaeIII GTNAC 4 cut(s) 20, 53, 160, 220
MalI GATC 6 cut(s) 134, 152, 416, 420, 490, 507
MboI GATC 6 cut(s) 132, 150, 414, 418, 488, 505
MboII GAAGA 3 cut(s) 240, 275, 424
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 1 cut(s) 505
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 234
MluI ACGCGT 1 cut(s) 157
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 6 cut(s) 100, 175, 220, 241, 451, 589
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 218
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 503
MspR9I CCNGG 2 cut(s) 143, 503
MunI CAATTG 1 cut(s) 234
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 1 cut(s) 159
NciI CCSGG 1 cut(s) 503
NcoI CCATGG 2 cut(s) 324, 513
NdeI CATATG 1 cut(s) 402
NdeII GATC 6 cut(s) 132, 150, 414, 418, 488, 505
NlaIII CATG 3 cut(s) 10, 328, 517
NlaIV GGNNCC 2 cut(s) 303, 507
NmuCI GTSAC 4 cut(s) 20, 53, 160, 220
OliI CACNNNNGTG 1 cut(s) 218
PceI AGGCCT 1 cut(s) 230
PfeI GAWTC 1 cut(s) 601
PflFI GACNNNGTC 1 cut(s) 200
PkrI GCNGC 3 cut(s) 212, 271, 525
PleI GAGTC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 2 cut(s) 303, 507
PstI CTGCAG 1 cut(s) 274
PsuI RGATCY 1 cut(s) 505
PsyI GACNNNGTC 1 cut(s) 200
RsaI GTAC 2 cut(s) 303, 344
RsaNI GTAC 2 cut(s) 302, 343
RseI CAYNNNNRTG 1 cut(s) 218
SatI GCNGC 3 cut(s) 211, 270, 524
Sau3AI GATC 6 cut(s) 132, 150, 414, 418, 488, 505
SchI GAGTC 1 cut(s) 177
ScrFI CCNGG 2 cut(s) 143, 503
SfaNI GCATC 1 cut(s) 163
SfcI CTRYAG 2 cut(s) 270, 527
SmiMI CAYNNNNRTG 1 cut(s) 218
Sse9I AATT 1 cut(s) 234
SseBI AGGCCT 1 cut(s) 230
SsiI CCGC 2 cut(s) 146, 211
StuI AGGCCT 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 141, 501
StyI CCWWGG 2 cut(s) 324, 513
TaiI ACGT 4 cut(s) 60, 87, 204, 319
TaqI TCGA 2 cut(s) 135, 417
TasI AATT 1 cut(s) 234
TatI WGTACW 1 cut(s) 342
TauI GCSGC 1 cut(s) 213
TfiI GAWTC 1 cut(s) 601
TscAI CASTG 2 cut(s) 25, 312
TseFI GTSAC 4 cut(s) 20, 53, 160, 220
TseI GCWGC 2 cut(s) 269, 523
Tsp45I GTSAC 4 cut(s) 20, 53, 160, 220
TspDTI ATGAA 1 cut(s) 327
TspRI CASTG 2 cut(s) 25, 312
Tth111I GACNNNGTC 1 cut(s) 200
ZraI GACGTC 2 cut(s) 85, 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.