Rroxscaffold_5G00356790

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009705
Physical Location & Seq
Forward (+)
36166030 .. 36167743
1714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00356790.1

Sequence Viewer

Length: 741 bp
ATGGCCATGAGAATAGCCAGTGACTATTGGGAAGTGGTGGGCGACGCTCTCTGTGACGTATGTGTCAACCCAGATTTTGTGGAATCCGTTTCTGAACCAGAAAACGAGGTGCTATGCACCACCAAGTTCTTGATCGATATCCAGGCGGTGATCAGACGCGTCACCTCCTGCGATGCCGAACGACTCTCAGACTTGGACGACGTCATCAGCCGCACGAGTGTGACTGAGGCCTCAATTGAACTCTACTCTTCTAACTCAGACGAAGAAGCTGCCGTCTCCAACGTACTCCGAGAGCTGAAGGTACCACTGAATGAACGTGCCCGCATGGTGCGGAGAATAATGTACAAAGCCTGCGAGATAGAAAATGAGAATAGGTGTTATGGAAGGAGAAAGATTGCTCATATGTTGGTGAAGATCGATCTTATTGTTGTTGATGATGATGGTGACGACACAGATGAGGATGGTATTGTGGAGTGCTGCCTTGATGAATCACATCCTTACCCGGATCGTTCCATGGTAGAGCAGCTGCAGAAAACTGGGCATCGAGCGTCGCGTGGTCCGACCACCGGTTCCGGCAGAGATTCGACGGCGTCAAGGATTGTTGGCTGCTGTGCGGCCGGTGCTTCCATTCCGAATGGGACATGGGTCGGGGACCTGGTATGGGTTGTGGGCCCGTCTTCAATTCTTGGGCCTCTCGTTGGTGGGCTTGTAGTATGTTTTGAGATTTTGTTCCGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

27.05

Weight (kDa)

4.56

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
AatII GACGTC 1 cut(s) 204
Acc65I GGTACC 1 cut(s) 301
AccB1I GGYRCC 1 cut(s) 301
AccII CGCG 2 cut(s) 159, 553
AciI CCGC 5 cut(s) 146, 211, 322, 331, 614
AclWI GGATC 1 cut(s) 513
AcoI YGGCCR 2 cut(s) 3, 615
AcuI CTGAAG 1 cut(s) 317
AcyI GRCGYC 2 cut(s) 201, 590
AfaI GTAC 3 cut(s) 285, 303, 344
AfiI CCNNNNNNNGG 3 cut(s) 566, 661, 698
AflIII ACRYGT 1 cut(s) 157
AgeI ACCGGT 1 cut(s) 566
AgsI TTSAA 2 cut(s) 239, 681
AjnI CCWGG 2 cut(s) 141, 654
AleI CACNNNNGTG 1 cut(s) 218
AluBI AGCT 3 cut(s) 269, 295, 526
AluI AGCT 3 cut(s) 269, 295, 526
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 1 cut(s) 513
AoxI GGCC 5 cut(s) 3, 228, 615, 670, 689
ApaI GGGCCC 1 cut(s) 674
ApeKI GCWGC 5 cut(s) 269, 477, 523, 526, 606
AsiGI ACCGGT 1 cut(s) 566
Asp718I GGTACC 1 cut(s) 301
AspS9I GGNCC 5 cut(s) 557, 652, 670, 671, 689
AsuC2I CCSGG 1 cut(s) 503
AsuHPI GGTGA 4 cut(s) 154, 160, 421, 455
AvaII GGWCC 2 cut(s) 557, 652
BaeGI GKGCMC 2 cut(s) 322, 674
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 301
BanII GRGCYC 1 cut(s) 674
BauI CACGAG 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 669
BbvI GCAGC 5 cut(s) 256, 464, 513, 535, 593
BccI CCATC 2 cut(s) 434, 455
BceAI ACGGC 2 cut(s) 257, 603
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 2 cut(s) 143, 656
BclI TGATCA 1 cut(s) 150
BcnI CCSGG 1 cut(s) 503
BcoDI GTCTC 1 cut(s) 280
BfmI CTRYAG 1 cut(s) 527
BisI GCNGC 7 cut(s) 211, 270, 478, 524, 527, 607, 615
BlsI GCNGC 7 cut(s) 212, 271, 479, 525, 528, 608, 616
Bme1390I CCNGG 3 cut(s) 143, 503, 656
Bme18I GGWCC 2 cut(s) 557, 652
BmgT120I GGNCC 5 cut(s) 557, 652, 670, 671, 689
BmiI GGNNCC 4 cut(s) 303, 571, 653, 672
BmrFI CCNGG 3 cut(s) 143, 503, 656
BmrI ACTGGG 1 cut(s) 546
BmsI GCATC 2 cut(s) 163, 550
BmuI ACTGGG 1 cut(s) 546
BoxI GACNNNNGTC 1 cut(s) 644
BpiI GAAGAC 1 cut(s) 669
BpuMI CCSGG 1 cut(s) 503
Bsa29I ATCGAT 2 cut(s) 135, 417
BsaBI GATNNNNATC 1 cut(s) 137
BsaHI GRCGYC 2 cut(s) 201, 590
BsaJI CCNNGG 1 cut(s) 513
BsaWI WCCGGW 1 cut(s) 566
Bsc4I CCNNNNNNNGG 3 cut(s) 566, 661, 698
Bse118I RCCGGY 2 cut(s) 566, 617
Bse1I ACTGG 2 cut(s) 18, 541
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 2 cut(s) 143, 656
BseCI ATCGAT 2 cut(s) 135, 417
BseDI CCNNGG 1 cut(s) 513
BseGI GGATG 2 cut(s) 466, 493
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 3 cut(s) 566, 661, 698
BseMII CTCAG 3 cut(s) 201, 216, 270
BseNI ACTGG 2 cut(s) 18, 541
BseSI GKGCMC 2 cut(s) 322, 674
BseX3I CGGCCG 1 cut(s) 615
BseXI GCAGC 5 cut(s) 256, 464, 513, 535, 593
Bsh1236I CGCG 2 cut(s) 159, 553
Bsh1285I CGRYCG 1 cut(s) 618
BshFI GGCC 5 cut(s) 5, 230, 617, 672, 691
BshNI GGYRCC 1 cut(s) 301
BshTI ACCGGT 1 cut(s) 566
BshVI ATCGAT 2 cut(s) 135, 417
BsiEI CGRYCG 1 cut(s) 618
BsiSI CCGG 4 cut(s) 503, 567, 573, 618
BslFI GGGAC 2 cut(s) 652, 665
BslI CCNNNNNNNGG 3 cut(s) 566, 661, 698
BsmAI GTCTC 1 cut(s) 280
BsmBI CGTCTC 1 cut(s) 280
BsmFI GGGAC 2 cut(s) 652, 665
BsnI GGCC 5 cut(s) 5, 230, 617, 672, 691
Bsp120I GGGCCC 1 cut(s) 670
Bsp1286I GDGCHC 2 cut(s) 322, 674
Bsp1407I TGTACA 1 cut(s) 342
Bsp143I GATC 5 cut(s) 132, 150, 414, 418, 505
Bsp19I CCATGG 1 cut(s) 513
BspACI CCGC 5 cut(s) 146, 211, 322, 331, 614
BspANI GGCC 5 cut(s) 5, 230, 617, 672, 691
BspCNI CTCAG 3 cut(s) 200, 217, 269
BspDI ATCGAT 2 cut(s) 135, 417
BspFNI CGCG 2 cut(s) 159, 553
BspLI GGNNCC 4 cut(s) 303, 571, 653, 672
BspMAI CTGCAG 1 cut(s) 531
BspPI GGATC 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 301
BsrFI RCCGGY 2 cut(s) 566, 617
BsrGI TGTACA 1 cut(s) 342
BsrI ACTGG 2 cut(s) 18, 541
BssAI RCCGGY 2 cut(s) 566, 617
BssECI CCNNGG 1 cut(s) 513
BssMI GATC 5 cut(s) 132, 150, 414, 418, 505
BssNI GRCGYC 2 cut(s) 201, 590
BssSI CACGAG 1 cut(s) 214
BssT1I CCWWGG 1 cut(s) 513
Bst2BI CACGAG 1 cut(s) 214
Bst2UI CCWGG 2 cut(s) 143, 656
Bst6I CTCTTC 1 cut(s) 253
BstACI GRCGYC 2 cut(s) 201, 590
BstAUI TGTACA 1 cut(s) 342
BstC8I GCNNGC 2 cut(s) 322, 352
BstDEI CTNAG 3 cut(s) 187, 225, 256
BstDSI CCRYGG 1 cut(s) 513
BstF5I GGATG 2 cut(s) 466, 493
BstFNI CGCG 2 cut(s) 159, 553
BstKTI GATC 5 cut(s) 135, 153, 417, 421, 508
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 5 cut(s) 132, 150, 414, 418, 505
BstMCI CGRYCG 1 cut(s) 618
BstMWI GCNNNNNNNGC 1 cut(s) 620
BstNI CCWGG 2 cut(s) 143, 656
BstPAI GACNNNNGTC 1 cut(s) 644
BstSCI CCNGG 3 cut(s) 141, 501, 654
BstSFI CTRYAG 1 cut(s) 527
BstSLI GKGCMC 2 cut(s) 322, 674
BstUI CGCG 2 cut(s) 159, 553
BstV1I GCAGC 5 cut(s) 256, 464, 513, 535, 593
BstV2I GAAGAC 1 cut(s) 669
BstZI CGGCCG 1 cut(s) 615
Bsu15I ATCGAT 2 cut(s) 135, 417
BsuRI GGCC 5 cut(s) 5, 230, 617, 672, 691
BsuTUI ATCGAT 2 cut(s) 135, 417
BtgI CCRYGG 1 cut(s) 513
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 466, 493
BtsIMutI CAGTG 2 cut(s) 25, 305
Cac8I GCNNGC 2 cut(s) 322, 352
Cfr10I RCCGGY 2 cut(s) 566, 617
Cfr13I GGNCC 5 cut(s) 557, 652, 670, 671, 689
ClaI ATCGAT 2 cut(s) 135, 417
CseI GACGC 5 cut(s) 53, 148, 165, 537, 579
CsiI ACCWGGT 1 cut(s) 654
Csp6I GTAC 3 cut(s) 284, 302, 343
CspAI ACCGGT 1 cut(s) 566
CviAII CATG 4 cut(s) 7, 325, 514, 642
CviQI GTAC 3 cut(s) 284, 302, 343
DdeI CTNAG 3 cut(s) 187, 225, 256
DpnI GATC 5 cut(s) 134, 152, 416, 420, 507
DpnII GATC 5 cut(s) 132, 150, 414, 418, 505
DrdI GACNNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 62
EaeI YGGCCR 2 cut(s) 3, 615
EagI CGGCCG 1 cut(s) 615
Eam1104I CTCTTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 253
EclXI CGGCCG 1 cut(s) 615
Eco130I CCWWGG 1 cut(s) 513
Eco147I AGGCCT 1 cut(s) 230
Eco24I GRGCYC 1 cut(s) 674
Eco32I GATATC 1 cut(s) 139
Eco47I GGWCC 2 cut(s) 557, 652
Eco52I CGGCCG 1 cut(s) 615
Eco57I CTGAAG 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 652
EcoRII CCWGG 2 cut(s) 141, 654
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 513
EcoT38I GRGCYC 1 cut(s) 674
ErhI CCWWGG 1 cut(s) 513
Esp3I CGTCTC 1 cut(s) 280
FaeI CATG 4 cut(s) 10, 328, 517, 645
FaqI GGGAC 2 cut(s) 652, 665
FatI CATG 4 cut(s) 6, 324, 513, 641
FauI CCCGC 1 cut(s) 329
FauNDI CATATG 1 cut(s) 402
FbaI TGATCA 1 cut(s) 150
Fnu4HI GCNGC 7 cut(s) 211, 270, 478, 524, 527, 607, 615
FokI GGATG 2 cut(s) 473, 480
FriOI GRGCYC 1 cut(s) 674
Fsp4HI GCNGC 7 cut(s) 211, 270, 478, 524, 527, 607, 615
GluI GCNGC 7 cut(s) 211, 270, 478, 524, 527, 607, 615
HaeIII GGCC 5 cut(s) 5, 230, 617, 672, 691
HapII CCGG 4 cut(s) 503, 567, 573, 618
HgaI GACGC 5 cut(s) 53, 148, 165, 537, 579
Hin1I GRCGYC 2 cut(s) 201, 590
Hin1II CATG 4 cut(s) 10, 328, 517, 645
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 4 cut(s) 83, 183, 488, 581
HpaII CCGG 4 cut(s) 503, 567, 573, 618
HphI GGTGA 4 cut(s) 154, 160, 421, 455
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 8 cut(s) 94, 155, 190, 259, 290, 561, 633, 734
Hpy188III TCNNGA 1 cut(s) 130
Hpy8I GTNNAC 1 cut(s) 67
Hpy99I CGWCG 4 cut(s) 47, 203, 553, 589
HpyAV CCTTC 2 cut(s) 292, 378
HpyCH4IV ACGT 4 cut(s) 57, 201, 282, 316
HpyCH4V TGCA 2 cut(s) 117, 529
HpyF10VI GCNNNNNNNGC 1 cut(s) 620
HpyF3I CTNAG 3 cut(s) 187, 225, 256
HpySE526I ACGT 4 cut(s) 57, 201, 282, 316
Hsp92I GRCGYC 2 cut(s) 201, 590
Hsp92II CATG 4 cut(s) 10, 328, 517, 645
KpnI GGTACC 1 cut(s) 305
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 5 cut(s) 132, 150, 414, 418, 505
Lsp1109I GCAGC 5 cut(s) 256, 464, 513, 535, 593
LweI GCATC 2 cut(s) 163, 550
MabI ACCWGGT 1 cut(s) 654
MaeII ACGT 4 cut(s) 57, 201, 282, 316
MaeIII GTNAC 5 cut(s) 20, 53, 160, 220, 443
MalI GATC 5 cut(s) 134, 152, 416, 420, 507
MboI GATC 5 cut(s) 132, 150, 414, 418, 505
MboII GAAGA 4 cut(s) 240, 275, 424, 669
MfeI CAATTG 1 cut(s) 234
MhlI GDGCHC 2 cut(s) 322, 674
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 234, 681
MluI ACGCGT 1 cut(s) 157
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 177
MmeI TCCRAC 2 cut(s) 303, 584
MnlI CCTC 6 cut(s) 100, 175, 220, 241, 451, 702
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 218
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 526
MspI CCGG 4 cut(s) 503, 567, 573, 618
MspR9I CCNGG 3 cut(s) 143, 503, 656
MunI CAATTG 1 cut(s) 234
MvaI CCWGG 2 cut(s) 143, 656
MvnI CGCG 2 cut(s) 159, 553
MwoI GCNNNNNNNGC 1 cut(s) 620
NciI CCSGG 1 cut(s) 503
NcoI CCATGG 1 cut(s) 513
NdeI CATATG 1 cut(s) 402
NdeII GATC 5 cut(s) 132, 150, 414, 418, 505
NlaIII CATG 4 cut(s) 10, 328, 517, 645
NlaIV GGNNCC 4 cut(s) 303, 571, 653, 672
NmuCI GTSAC 5 cut(s) 20, 53, 160, 220, 443
OliI CACNNNNGTG 1 cut(s) 218
PceI AGGCCT 1 cut(s) 230
PcsI WCGNNNNNNNCGW 2 cut(s) 550, 557
PfeI GAWTC 3 cut(s) 83, 488, 581
PflFI GACNNNGTC 2 cut(s) 200, 589
PinAI ACCGGT 1 cut(s) 566
PkrI GCNGC 7 cut(s) 212, 271, 479, 525, 528, 608, 616
PleI GAGTC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 177
PpuMI RGGWCCY 1 cut(s) 652
PshAI GACNNNNGTC 1 cut(s) 644
Psp5II RGGWCCY 1 cut(s) 652
Psp6I CCWGG 2 cut(s) 141, 654
PspGI CCWGG 2 cut(s) 141, 654
PspN4I GGNNCC 4 cut(s) 303, 571, 653, 672
PspOMI GGGCCC 1 cut(s) 670
PspPI GGNCC 5 cut(s) 557, 652, 670, 671, 689
PspPPI RGGWCCY 1 cut(s) 652
PstI CTGCAG 1 cut(s) 531
PsyI GACNNNGTC 2 cut(s) 200, 589
PvuII CAGCTG 1 cut(s) 526
RsaI GTAC 3 cut(s) 285, 303, 344
RsaNI GTAC 3 cut(s) 284, 302, 343
RseI CAYNNNNRTG 1 cut(s) 218
SatI GCNGC 7 cut(s) 211, 270, 478, 524, 527, 607, 615
Sau3AI GATC 5 cut(s) 132, 150, 414, 418, 505
Sau96I GGNCC 5 cut(s) 557, 652, 670, 671, 689
SchI GAGTC 1 cut(s) 177
ScrFI CCNGG 3 cut(s) 143, 503, 656
SduI GDGCHC 2 cut(s) 322, 674
SexAI ACCWGGT 1 cut(s) 654
SfaNI GCATC 2 cut(s) 163, 550
SfcI CTRYAG 1 cut(s) 527
SinI GGWCC 2 cut(s) 557, 652
SmiMI CAYNNNNRTG 1 cut(s) 218
Sse9I AATT 2 cut(s) 234, 681
SseBI AGGCCT 1 cut(s) 230
SsiI CCGC 5 cut(s) 146, 211, 322, 331, 614
StuI AGGCCT 1 cut(s) 230
StyD4I CCNGG 3 cut(s) 141, 501, 654
StyI CCWWGG 1 cut(s) 513
TaiI ACGT 4 cut(s) 60, 204, 285, 319
TaqI TCGA 4 cut(s) 135, 417, 544, 584
TasI AATT 2 cut(s) 234, 681
TatI WGTACW 1 cut(s) 342
TauI GCSGC 2 cut(s) 213, 617
TfiI GAWTC 3 cut(s) 83, 488, 581
TscAI CASTG 2 cut(s) 25, 312
TseFI GTSAC 5 cut(s) 20, 53, 160, 220, 443
TseI GCWGC 5 cut(s) 269, 477, 523, 526, 606
Tsp45I GTSAC 5 cut(s) 20, 53, 160, 220, 443
TspDTI ATGAA 2 cut(s) 327, 501
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 2 cut(s) 25, 312
Tth111I GACNNNGTC 2 cut(s) 200, 589
VpaK11BI GGWCC 2 cut(s) 557, 652
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.