Rroxscaffold_7G00189160

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009705
Physical Location & Seq
Forward (+)
28901635 .. 28904022
2388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00189160.1

Sequence Viewer

Length: 675 bp
ATGCAGGAGAGAAGAGATACAAGTGATGCTGTGGCAGAGTGGCTAACACAGTTTCTAAGATTGAACATGGCCATGAGAATAGCCAGCGACTATTGGGCAGTGGTGGGCGACGCTCTCTGTGACGTATGTGTCAACCCAGATTTTGTGGACGCCGTTTCTGAACCAGAAAACGAGGTGCTATGCACCACCAAGTTCTTGATCGATATCCAGGCGGTGATCAGACGCATCACCTCCTGCGATGCCGAACGACTCTCAGACTTGGACGACGTCATCAGCCGCACGAGTGTGACTGAGGCCTCAATTGAACTCTACTCTTCTAACTCAGACGAAGAAGCTGCCGTCTCCAACGTACTCCGAGAGCTGAAGGTACCACTGAATGAACGTGCCCGCATGGTGCGGAGAATAATGTACGAAGCCTGCGAGATAGAAAATGAGAATAGGTGTTATGGAAGGAGAAAGATTGCTCATATGTTGGTGAAGATCGATCTTATTGTTGTTGATGATGATGGTGACGACACAGATGAGGATGGTATTGTGGAGTGCTGCCTTGATGAATCACATCCTTACCCGGATCGTTCCATGGTAGAGCAGCTGCAGAAAACTGGGGTTTGCCCTATGTGTGATGATGATAGGAAAGTGTGGTTGACAAAGATGGAGAACGAGGGGATTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

224

Amino Acids

25.55

Weight (kDa)

4.35

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AatII GACGTC 1 cut(s) 270
Acc65I GGTACC 1 cut(s) 367
AccB1I GGYRCC 1 cut(s) 367
AciI CCGC 4 cut(s) 212, 277, 388, 397
AclWI GGATC 1 cut(s) 579
AcoI YGGCCR 1 cut(s) 69
AcuI CTGAAG 1 cut(s) 383
AcyI GRCGYC 2 cut(s) 150, 267
AfaI GTAC 3 cut(s) 351, 369, 410
AgsI TTSAA 2 cut(s) 64, 305
AjnI CCWGG 1 cut(s) 207
AleI CACNNNNGTG 1 cut(s) 284
AluBI AGCT 3 cut(s) 335, 361, 592
AluI AGCT 3 cut(s) 335, 361, 592
Alw26I GTCTC 1 cut(s) 346
AlwI GGATC 1 cut(s) 579
AoxI GGCC 2 cut(s) 69, 294
ApeKI GCWGC 4 cut(s) 335, 543, 589, 592
Asp718I GGTACC 1 cut(s) 367
AsuC2I CCSGG 1 cut(s) 569
AsuHPI GGTGA 4 cut(s) 220, 226, 487, 521
BaeGI GKGCMC 1 cut(s) 388
BalI TGGCCA 1 cut(s) 71
BanI GGYRCC 1 cut(s) 367
BauI CACGAG 1 cut(s) 280
BbvI GCAGC 4 cut(s) 322, 530, 579, 601
BccI CCATC 3 cut(s) 500, 521, 646
BceAI ACGGC 2 cut(s) 137, 323
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 1 cut(s) 209
BclI TGATCA 1 cut(s) 216
BcnI CCSGG 1 cut(s) 569
BcoDI GTCTC 1 cut(s) 346
BfmI CTRYAG 1 cut(s) 593
BisI GCNGC 5 cut(s) 277, 336, 544, 590, 593
BlsI GCNGC 5 cut(s) 278, 337, 545, 591, 594
Bme1390I CCNGG 2 cut(s) 209, 569
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 2 cut(s) 209, 569
BmrI ACTGGG 1 cut(s) 612
BmsI GCATC 3 cut(s) 16, 229, 234
BmuI ACTGGG 1 cut(s) 612
BpuMI CCSGG 1 cut(s) 569
Bsa29I ATCGAT 2 cut(s) 201, 483
BsaBI GATNNNNATC 1 cut(s) 203
BsaHI GRCGYC 2 cut(s) 150, 267
BsaJI CCNNGG 1 cut(s) 579
Bse1I ACTGG 1 cut(s) 607
Bse8I GATNNNNATC 1 cut(s) 203
BseBI CCWGG 1 cut(s) 209
BseCI ATCGAT 2 cut(s) 201, 483
BseDI CCNNGG 1 cut(s) 579
BseGI GGATG 2 cut(s) 532, 559
BseJI GATNNNNATC 1 cut(s) 203
BseMII CTCAG 3 cut(s) 267, 282, 336
BseNI ACTGG 1 cut(s) 607
BseSI GKGCMC 1 cut(s) 388
BseXI GCAGC 4 cut(s) 322, 530, 579, 601
BshFI GGCC 2 cut(s) 71, 296
BshNI GGYRCC 1 cut(s) 367
BshVI ATCGAT 2 cut(s) 201, 483
BsiSI CCGG 1 cut(s) 569
BsmAI GTCTC 1 cut(s) 346
BsmBI CGTCTC 1 cut(s) 346
BsnI GGCC 2 cut(s) 71, 296
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 5 cut(s) 198, 216, 480, 484, 571
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 4 cut(s) 212, 277, 388, 397
BspANI GGCC 2 cut(s) 71, 296
BspCNI CTCAG 3 cut(s) 266, 283, 335
BspDI ATCGAT 2 cut(s) 201, 483
BspLI GGNNCC 1 cut(s) 369
BspMAI CTGCAG 1 cut(s) 597
BspPI GGATC 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 367
BsrI ACTGG 1 cut(s) 607
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 5 cut(s) 198, 216, 480, 484, 571
BssNI GRCGYC 2 cut(s) 150, 267
BssSI CACGAG 1 cut(s) 280
BssT1I CCWWGG 1 cut(s) 579
Bst2BI CACGAG 1 cut(s) 280
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 1 cut(s) 51
Bst6I CTCTTC 2 cut(s) 7, 319
BstACI GRCGYC 2 cut(s) 150, 267
BstC8I GCNNGC 3 cut(s) 85, 388, 418
BstDEI CTNAG 4 cut(s) 56, 253, 291, 322
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 2 cut(s) 532, 559
BstKTI GATC 5 cut(s) 201, 219, 483, 487, 574
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 5 cut(s) 198, 216, 480, 484, 571
BstNI CCWGG 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 207, 567
BstSFI CTRYAG 1 cut(s) 593
BstSLI GKGCMC 1 cut(s) 388
BstV1I GCAGC 4 cut(s) 322, 530, 579, 601
Bsu15I ATCGAT 2 cut(s) 201, 483
BsuRI GGCC 2 cut(s) 71, 296
BsuTUI ATCGAT 2 cut(s) 201, 483
BtgI CCRYGG 1 cut(s) 579
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 2 cut(s) 532, 559
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 105, 371
Cac8I GCNNGC 3 cut(s) 85, 388, 418
ClaI ATCGAT 2 cut(s) 201, 483
CseI GACGC 3 cut(s) 119, 158, 231
Csp6I GTAC 3 cut(s) 350, 368, 409
CviAII CATG 4 cut(s) 67, 73, 391, 580
CviJI RGCY 9 cut(s) 43, 71, 83, 276, 296, 335, 361, 416, 592
CviKI_1 RGCY 9 cut(s) 43, 71, 83, 276, 296, 335, 361, 416, 592
CviQI GTAC 3 cut(s) 350, 368, 409
DdeI CTNAG 4 cut(s) 56, 253, 291, 322
DpnI GATC 5 cut(s) 200, 218, 482, 486, 573
DpnII GATC 5 cut(s) 198, 216, 480, 484, 571
DrdI GACNNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 7, 319
EarI CTCTTC 2 cut(s) 7, 319
Eco130I CCWWGG 1 cut(s) 579
Eco147I AGGCCT 1 cut(s) 296
Eco32I GATATC 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 207
EcoRV GATATC 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 579
Esp3I CGTCTC 1 cut(s) 346
FaeI CATG 4 cut(s) 70, 76, 394, 583
FatI CATG 4 cut(s) 66, 72, 390, 579
FauI CCCGC 1 cut(s) 395
FauNDI CATATG 1 cut(s) 468
FbaI TGATCA 1 cut(s) 216
Fnu4HI GCNGC 5 cut(s) 277, 336, 544, 590, 593
FokI GGATG 2 cut(s) 539, 546
Fsp4HI GCNGC 5 cut(s) 277, 336, 544, 590, 593
GluI GCNGC 5 cut(s) 277, 336, 544, 590, 593
HaeIII GGCC 2 cut(s) 71, 296
HapII CCGG 1 cut(s) 569
HgaI GACGC 3 cut(s) 119, 158, 231
Hin1I GRCGYC 2 cut(s) 150, 267
Hin1II CATG 4 cut(s) 70, 76, 394, 583
HincII GTYRAC 2 cut(s) 133, 645
HindII GTYRAC 2 cut(s) 133, 645
HinfI GANTC 3 cut(s) 249, 554, 667
HpaII CCGG 1 cut(s) 569
HphI GGTGA 4 cut(s) 220, 226, 487, 521
Hpy166II GTNNAC 3 cut(s) 133, 148, 645
Hpy188I TCNGA 5 cut(s) 160, 221, 256, 325, 356
Hpy188III TCNNGA 1 cut(s) 196
Hpy8I GTNNAC 3 cut(s) 133, 148, 645
Hpy99I CGWCG 2 cut(s) 113, 269
HpyAV CCTTC 2 cut(s) 358, 444
HpyCH4III ACNGT 1 cut(s) 51
HpyCH4IV ACGT 4 cut(s) 123, 267, 348, 382
HpyCH4V TGCA 3 cut(s) 4, 183, 595
HpyF3I CTNAG 4 cut(s) 56, 253, 291, 322
HpySE526I ACGT 4 cut(s) 123, 267, 348, 382
Hsp92I GRCGYC 2 cut(s) 150, 267
Hsp92II CATG 4 cut(s) 70, 76, 394, 583
KpnI GGTACC 1 cut(s) 371
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 5 cut(s) 198, 216, 480, 484, 571
LpnPI CCDG 9 cut(s) 97, 150, 177, 194, 221, 247, 430, 582, 588
Lsp1109I GCAGC 4 cut(s) 322, 530, 579, 601
LweI GCATC 3 cut(s) 16, 229, 234
MaeII ACGT 4 cut(s) 123, 267, 348, 382
MaeIII GTNAC 3 cut(s) 119, 286, 509
MalI GATC 5 cut(s) 200, 218, 482, 486, 573
MboI GATC 5 cut(s) 198, 216, 480, 484, 571
MboII GAAGA 4 cut(s) 24, 306, 341, 490
MfeI CAATTG 1 cut(s) 300
MhlI GDGCHC 1 cut(s) 388
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 1 cut(s) 300
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 1 cut(s) 243
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 6 cut(s) 166, 241, 286, 307, 517, 655
Mox20I TGGCCA 1 cut(s) 71
MscI TGGCCA 1 cut(s) 71
MslI CAYNNNNRTG 2 cut(s) 71, 284
Msp20I TGGCCA 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 592
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 2 cut(s) 209, 569
MunI CAATTG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 209
NciI CCSGG 1 cut(s) 569
NcoI CCATGG 1 cut(s) 579
NdeI CATATG 1 cut(s) 468
NdeII GATC 5 cut(s) 198, 216, 480, 484, 571
NlaIII CATG 4 cut(s) 70, 76, 394, 583
NlaIV GGNNCC 1 cut(s) 369
NmuCI GTSAC 3 cut(s) 119, 286, 509
OliI CACNNNNGTG 1 cut(s) 284
PceI AGGCCT 1 cut(s) 296
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PfeI GAWTC 2 cut(s) 554, 667
PflFI GACNNNGTC 1 cut(s) 266
PkrI GCNGC 5 cut(s) 278, 337, 545, 591, 594
PleI GAGTC 1 cut(s) 243
PpsI GAGTC 1 cut(s) 243
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 1 cut(s) 369
PstI CTGCAG 1 cut(s) 597
PsyI GACNNNGTC 1 cut(s) 266
PvuII CAGCTG 1 cut(s) 592
RsaI GTAC 3 cut(s) 351, 369, 410
RsaNI GTAC 3 cut(s) 350, 368, 409
RseI CAYNNNNRTG 2 cut(s) 71, 284
SatI GCNGC 5 cut(s) 277, 336, 544, 590, 593
Sau3AI GATC 5 cut(s) 198, 216, 480, 484, 571
SchI GAGTC 1 cut(s) 243
ScrFI CCNGG 2 cut(s) 209, 569
SduI GDGCHC 1 cut(s) 388
SfaNI GCATC 3 cut(s) 16, 229, 234
SfcI CTRYAG 1 cut(s) 593
SmiMI CAYNNNNRTG 2 cut(s) 71, 284
Sse9I AATT 1 cut(s) 300
SseBI AGGCCT 1 cut(s) 296
SsiI CCGC 4 cut(s) 212, 277, 388, 397
StuI AGGCCT 1 cut(s) 296
StyD4I CCNGG 2 cut(s) 207, 567
StyI CCWWGG 1 cut(s) 579
TaaI ACNGT 1 cut(s) 51
TaiI ACGT 4 cut(s) 126, 270, 351, 385
TaqI TCGA 2 cut(s) 201, 483
TasI AATT 1 cut(s) 300
TauI GCSGC 1 cut(s) 279
TfiI GAWTC 2 cut(s) 554, 667
TscAI CASTG 2 cut(s) 105, 378
TseFI GTSAC 3 cut(s) 119, 286, 509
TseI GCWGC 4 cut(s) 335, 543, 589, 592
Tsp45I GTSAC 3 cut(s) 119, 286, 509
TspDTI ATGAA 2 cut(s) 393, 567
TspRI CASTG 2 cut(s) 105, 378
Tth111I GACNNNGTC 1 cut(s) 266
ZraI GACGTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.