Rh4BG173600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0009705
Physical Location & Seq
Forward (+)
30976274 .. 30977391
1118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG173600.1

Sequence Viewer

Length: 609 bp
ATGGCCATGAGAATGGCCAGTGACTATTGGGAAGTGGTGGGCGACGCTCTCTGTGACGTACGTGTCAACCCACATTTTGTGGACGTCGTTTCTGAACCAGAAAACGAGGTGCTATGCACCACCAAGTTCTTGATCAATTTCCTGGTGGTGATCAGACGCGTCACCTCCTGCGATGCCGAACGACTCTCAGACTTGGACGACGTCATCAGCCGCACGAGTGTGACTGAGGCCTCAATCGAACTCGACTCTTCTAACTCAGACAAAGCAGCTGCCGTCTCCGACGCGCTCCGAGAGCTGAAGGTACCACTGAATGAACGTGTCCTCATGGTGCAGAGAATAATGTATAAAGCCTGCGAGATAGAAAATGAGAATAGGTGTTATGGAAGCAGAAAGATTGCTCATATGTCGGTGAAGATCGATCTTATTGTTGTTGATGATGATGGTGACGACACAGATGAGGATGGTATTGTGGAGTGCTGTCTTGATGGATCACATCCTTACCCGGATCCTTCCATGATAGAGCAGCTACAGAAAACTGGGGTTTGCCCTATGTGTGATGATGATAGGAAAGTGTGCTTGACAAAGATGGAGAATGAGGGGATTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

22.63

Weight (kDa)

4.37

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
AatII GACGTC 2 cut(s) 87, 204
Acc65I GGTACC 1 cut(s) 301
AccB1I GGYRCC 1 cut(s) 301
AccII CGCG 2 cut(s) 159, 284
AciI CCGC 1 cut(s) 211
AclWI GGATC 3 cut(s) 496, 500, 513
AcoI YGGCCR 2 cut(s) 3, 15
AcuI CTGAAG 1 cut(s) 317
AcyI GRCGYC 2 cut(s) 84, 201
AfaI GTAC 2 cut(s) 60, 303
AflIII ACRYGT 3 cut(s) 61, 157, 316
AjnI CCWGG 1 cut(s) 141
AleI CACNNNNGTG 1 cut(s) 218
AluBI AGCT 3 cut(s) 269, 295, 526
AluI AGCT 3 cut(s) 269, 295, 526
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 3 cut(s) 496, 500, 513
AoxI GGCC 3 cut(s) 3, 15, 228
ApeKI GCWGC 3 cut(s) 266, 269, 523
Asp718I GGTACC 1 cut(s) 301
AspLEI GCGC 1 cut(s) 286
AsuC2I CCSGG 1 cut(s) 503
AsuHPI GGTGA 4 cut(s) 154, 160, 421, 455
BalI TGGCCA 2 cut(s) 5, 17
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 301
BauI CACGAG 1 cut(s) 214
BbvI GCAGC 3 cut(s) 256, 278, 535
BccI CCATC 4 cut(s) 434, 455, 479, 580
BceAI ACGGC 1 cut(s) 257
BciT130I CCWGG 1 cut(s) 143
BclI TGATCA 2 cut(s) 132, 150
BcnI CCSGG 1 cut(s) 503
BcoDI GTCTC 1 cut(s) 280
BfmI CTRYAG 1 cut(s) 527
BisI GCNGC 4 cut(s) 211, 267, 270, 524
BlsI GCNGC 4 cut(s) 212, 268, 271, 525
Bme1390I CCNGG 2 cut(s) 143, 503
BmiI GGNNCC 2 cut(s) 303, 507
BmrFI CCNGG 2 cut(s) 143, 503
BmrI ACTGGG 1 cut(s) 546
BmsI GCATC 1 cut(s) 163
BmuI ACTGGG 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 503
Bsa29I ATCGAT 1 cut(s) 417
BsaAI YACGTR 1 cut(s) 62
BsaHI GRCGYC 2 cut(s) 84, 201
Bse1I ACTGG 2 cut(s) 18, 541
BseBI CCWGG 1 cut(s) 143
BseCI ATCGAT 1 cut(s) 417
BseGI GGATG 2 cut(s) 466, 493
BseMII CTCAG 3 cut(s) 201, 216, 270
BseNI ACTGG 2 cut(s) 18, 541
BseXI GCAGC 3 cut(s) 256, 278, 535
BsgI GTGCAG 1 cut(s) 350
Bsh1236I CGCG 2 cut(s) 159, 284
BshFI GGCC 3 cut(s) 5, 17, 230
BshNI GGYRCC 1 cut(s) 301
BshVI ATCGAT 1 cut(s) 417
BsiSI CCGG 1 cut(s) 503
BsiWI CGTACG 1 cut(s) 58
BsmAI GTCTC 1 cut(s) 280
BsmBI CGTCTC 1 cut(s) 280
BsnI GGCC 3 cut(s) 5, 17, 230
Bsp143I GATC 6 cut(s) 132, 150, 414, 418, 488, 505
BspACI CCGC 1 cut(s) 211
BspANI GGCC 3 cut(s) 5, 17, 230
BspCNI CTCAG 3 cut(s) 200, 217, 269
BspDI ATCGAT 1 cut(s) 417
BspFNI CGCG 2 cut(s) 159, 284
BspLI GGNNCC 2 cut(s) 303, 507
BspPI GGATC 3 cut(s) 496, 500, 513
BspT107I GGYRCC 1 cut(s) 301
BsrI ACTGG 2 cut(s) 18, 541
BssMI GATC 6 cut(s) 132, 150, 414, 418, 488, 505
BssNI GRCGYC 2 cut(s) 84, 201
BssSI CACGAG 1 cut(s) 214
Bst2BI CACGAG 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 253
BstACI GRCGYC 2 cut(s) 84, 201
BstBAI YACGTR 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 352
BstDEI CTNAG 3 cut(s) 187, 225, 256
BstF5I GGATG 2 cut(s) 466, 493
BstFNI CGCG 2 cut(s) 159, 284
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 6 cut(s) 135, 153, 417, 421, 491, 508
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 6 cut(s) 132, 150, 414, 418, 488, 505
BstMWI GCNNNNNNNGC 1 cut(s) 292
BstNI CCWGG 1 cut(s) 143
BstSCI CCNGG 2 cut(s) 141, 501
BstSFI CTRYAG 1 cut(s) 527
BstUI CGCG 2 cut(s) 159, 284
BstV1I GCAGC 3 cut(s) 256, 278, 535
BstX2I RGATCY 1 cut(s) 505
BstXI CCANNNNNNTGG 1 cut(s) 13
BstYI RGATCY 1 cut(s) 505
Bsu15I ATCGAT 1 cut(s) 417
BsuRI GGCC 3 cut(s) 5, 17, 230
BsuTUI ATCGAT 1 cut(s) 417
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 466, 493
BtsIMutI CAGTG 2 cut(s) 25, 305
Cac8I GCNNGC 1 cut(s) 352
CfoI GCGC 1 cut(s) 286
ClaI ATCGAT 1 cut(s) 417
CseI GACGC 4 cut(s) 53, 148, 165, 290
Csp6I GTAC 2 cut(s) 59, 302
CviAII CATG 3 cut(s) 7, 325, 514
CviJI RGCY 8 cut(s) 5, 17, 210, 230, 269, 295, 350, 526
CviKI_1 RGCY 8 cut(s) 5, 17, 210, 230, 269, 295, 350, 526
CviQI GTAC 2 cut(s) 59, 302
DdeI CTNAG 3 cut(s) 187, 225, 256
DpnI GATC 6 cut(s) 134, 152, 416, 420, 490, 507
DpnII GATC 6 cut(s) 132, 150, 414, 418, 488, 505
DrdI GACNNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 62
EaeI YGGCCR 2 cut(s) 3, 15
Eam1104I CTCTTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 253
Eco147I AGGCCT 1 cut(s) 230
Eco57I CTGAAG 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 141
Esp3I CGTCTC 1 cut(s) 280
FaeI CATG 3 cut(s) 10, 328, 517
FatI CATG 3 cut(s) 6, 324, 513
FauNDI CATATG 1 cut(s) 402
FbaI TGATCA 2 cut(s) 132, 150
Fnu4HI GCNGC 4 cut(s) 211, 267, 270, 524
FokI GGATG 2 cut(s) 473, 480
Fsp4HI GCNGC 4 cut(s) 211, 267, 270, 524
GlaI GCGC 1 cut(s) 285
GluI GCNGC 4 cut(s) 211, 267, 270, 524
HaeIII GGCC 3 cut(s) 5, 17, 230
HapII CCGG 1 cut(s) 503
HgaI GACGC 4 cut(s) 53, 148, 165, 290
HhaI GCGC 1 cut(s) 286
Hin1I GRCGYC 2 cut(s) 84, 201
Hin1II CATG 3 cut(s) 10, 328, 517
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 3 cut(s) 183, 245, 601
HpaII CCGG 1 cut(s) 503
HphI GGTGA 4 cut(s) 154, 160, 421, 455
Hpy166II GTNNAC 2 cut(s) 67, 82
Hpy188I TCNGA 6 cut(s) 94, 155, 190, 259, 280, 290
Hpy188III TCNNGA 2 cut(s) 130, 482
Hpy8I GTNNAC 2 cut(s) 67, 82
Hpy99I CGWCG 4 cut(s) 47, 89, 203, 284
HpyAV CCTTC 2 cut(s) 292, 519
HpyCH4IV ACGT 5 cut(s) 57, 61, 84, 201, 316
HpyCH4V TGCA 2 cut(s) 117, 331
HpyF10VI GCNNNNNNNGC 1 cut(s) 292
HpyF3I CTNAG 3 cut(s) 187, 225, 256
HpySE526I ACGT 5 cut(s) 57, 61, 84, 201, 316
Hsp92I GRCGYC 2 cut(s) 84, 201
Hsp92II CATG 3 cut(s) 10, 328, 517
HspAI GCGC 1 cut(s) 284
KpnI GGTACC 1 cut(s) 305
Ksp22I TGATCA 2 cut(s) 132, 150
Kzo9I GATC 6 cut(s) 132, 150, 414, 418, 488, 505
LmnI GCTCC 1 cut(s) 291
LpnPI CCDG 8 cut(s) 31, 111, 128, 155, 181, 364, 516, 522
Lsp1109I GCAGC 3 cut(s) 256, 278, 535
LweI GCATC 1 cut(s) 163
MaeII ACGT 5 cut(s) 57, 61, 84, 201, 316
MaeIII GTNAC 5 cut(s) 20, 53, 160, 220, 443
MalI GATC 6 cut(s) 134, 152, 416, 420, 490, 507
MboI GATC 6 cut(s) 132, 150, 414, 418, 488, 505
MboII GAAGA 2 cut(s) 240, 424
MflI RGATCY 1 cut(s) 505
MlsI TGGCCA 2 cut(s) 5, 17
MluCI AATT 1 cut(s) 136
MluI ACGCGT 1 cut(s) 157
MluNI TGGCCA 2 cut(s) 5, 17
MlyI GAGTC 2 cut(s) 177, 239
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 7 cut(s) 100, 175, 220, 241, 332, 451, 589
Mox20I TGGCCA 2 cut(s) 5, 17
MscI TGGCCA 2 cut(s) 5, 17
MslI CAYNNNNRTG 2 cut(s) 11, 218
Msp20I TGGCCA 2 cut(s) 5, 17
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 1 cut(s) 503
MspR9I CCNGG 2 cut(s) 143, 503
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 2 cut(s) 159, 284
MwoI GCNNNNNNNGC 1 cut(s) 292
NciI CCSGG 1 cut(s) 503
NdeI CATATG 1 cut(s) 402
NdeII GATC 6 cut(s) 132, 150, 414, 418, 488, 505
NlaIII CATG 3 cut(s) 10, 328, 517
NlaIV GGNNCC 2 cut(s) 303, 507
NmuCI GTSAC 5 cut(s) 20, 53, 160, 220, 443
OliI CACNNNNGTG 1 cut(s) 218
PceI AGGCCT 1 cut(s) 230
PfeI GAWTC 1 cut(s) 601
Pfl23II CGTACG 1 cut(s) 58
PflFI GACNNNGTC 1 cut(s) 200
PkrI GCNGC 4 cut(s) 212, 268, 271, 525
PleI GAGTC 2 cut(s) 177, 239
PpsI GAGTC 2 cut(s) 177, 239
Ppu21I YACGTR 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspLI CGTACG 1 cut(s) 58
PspN4I GGNNCC 2 cut(s) 303, 507
PsuI RGATCY 1 cut(s) 505
PsyI GACNNNGTC 1 cut(s) 200
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 2 cut(s) 60, 303
RsaNI GTAC 2 cut(s) 59, 302
RseI CAYNNNNRTG 2 cut(s) 11, 218
SatI GCNGC 4 cut(s) 211, 267, 270, 524
Sau3AI GATC 6 cut(s) 132, 150, 414, 418, 488, 505
SchI GAGTC 2 cut(s) 177, 239
ScrFI CCNGG 2 cut(s) 143, 503
SfaNI GCATC 1 cut(s) 163
SfcI CTRYAG 1 cut(s) 527
SmiMI CAYNNNNRTG 2 cut(s) 11, 218
Sse9I AATT 1 cut(s) 136
SseBI AGGCCT 1 cut(s) 230
SsiI CCGC 1 cut(s) 211
StuI AGGCCT 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 141, 501
TaiI ACGT 5 cut(s) 60, 64, 87, 204, 319
TaqI TCGA 3 cut(s) 237, 243, 417
TasI AATT 1 cut(s) 136
TauI GCSGC 1 cut(s) 213
TfiI GAWTC 1 cut(s) 601
TscAI CASTG 2 cut(s) 25, 312
TseFI GTSAC 5 cut(s) 20, 53, 160, 220, 443
TseI GCWGC 3 cut(s) 266, 269, 523
Tsp45I GTSAC 5 cut(s) 20, 53, 160, 220, 443
TspDTI ATGAA 1 cut(s) 327
TspRI CASTG 2 cut(s) 25, 312
Tth111I GACNNNGTC 1 cut(s) 200
ZraI GACGTC 2 cut(s) 85, 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.