Rh4CG184600

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009705
Physical Location & Seq
Forward (+)
39772706 .. 39784508
11803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG184600.1

Sequence Viewer

Length: 672 bp
ATGGAGTGGGACTTCCTATCCCCAACCGTCTGCCTATGCCCTTTTGGGAGGAGATTGAACATGGCCATGAGAATAGCCAGTGACCATTGGGAAGTGGTGGGCGACGCTCTCTGTGACGTATGTGTCAACCCAGATTTTGTGGACGTCGTTTCTGAACCAGAAAACGAGGTGCTATGCACCACCAAGTTCTTGATCGATATCCAGGCTGTGATCAGACGCGTCACCTCCTGCGATGCCGAACGACTCTCAGACTTGGACGACGTCATCAGCCGCACGAGTGTGACTGAGGCCTCAATTGAACTCTACTCTTCTAACTCAGACGAAGAAGCTGCAGTCTCCAACGCACTCCGAGAGCTGAAGGTACCACTGAATGAACGTGTCCCCATGGTGCGGAGAATAATGTACAAAGCCTGCGAGATAGAAAATGAGAATAGGTGTTATGGAAGCAGAAAGATTGCTCATATGTCGGTGAAGATCGATCTTATTGTTGTTGATGATGATGGTGGCGACACAGATGAGGATGGTATTGTGGAGTGCTGTCTTGATGGATCACATCCTTACCCGGATCCTTCCATGGTAGAGCAGCTACAGAAAACTGGGGTTTGCCCTATGTGTGATGATGATAGGGAAGTGTACTGCTTGACAAAGATGGAGAATGAGGGGATTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

223

Amino Acids

25.04

Weight (kDa)

4.3

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AatII GACGTC 2 cut(s) 147, 264
Acc65I GGTACC 1 cut(s) 361
AccB1I GGYRCC 1 cut(s) 361
AccII CGCG 1 cut(s) 219
AciI CCGC 2 cut(s) 271, 391
AclWI GGATC 3 cut(s) 556, 560, 573
AcoI YGGCCR 1 cut(s) 63
AcuI CTGAAG 1 cut(s) 377
AcyI GRCGYC 2 cut(s) 144, 261
AfaI GTAC 3 cut(s) 363, 404, 635
AfiI CCNNNNNNNGG 1 cut(s) 390
AflIII ACRYGT 2 cut(s) 217, 376
AgsI TTSAA 2 cut(s) 58, 299
AjnI CCWGG 1 cut(s) 201
AleI CACNNNNGTG 1 cut(s) 278
AluBI AGCT 3 cut(s) 329, 355, 586
AluI AGCT 3 cut(s) 329, 355, 586
Alw26I GTCTC 1 cut(s) 340
AlwI GGATC 3 cut(s) 556, 560, 573
AoxI GGCC 2 cut(s) 63, 288
ApeKI GCWGC 2 cut(s) 329, 583
Asp718I GGTACC 1 cut(s) 361
AsuC2I CCSGG 1 cut(s) 563
AsuHPI GGTGA 2 cut(s) 214, 481
BalI TGGCCA 1 cut(s) 65
BamHI GGATCC 1 cut(s) 565
BanI GGYRCC 1 cut(s) 361
BauI CACGAG 1 cut(s) 274
BbvI GCAGC 2 cut(s) 316, 595
BccI CCATC 4 cut(s) 494, 515, 539, 643
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 1 cut(s) 210
BcnI CCSGG 1 cut(s) 563
BcoDI GTCTC 1 cut(s) 340
BfmI CTRYAG 2 cut(s) 330, 587
BisI GCNGC 3 cut(s) 271, 330, 584
BlsI GCNGC 3 cut(s) 272, 331, 585
Bme1390I CCNGG 2 cut(s) 203, 563
BmiI GGNNCC 2 cut(s) 363, 567
BmrFI CCNGG 2 cut(s) 203, 563
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 1 cut(s) 223
BmuI ACTGGG 1 cut(s) 606
BpuMI CCSGG 1 cut(s) 563
Bsa29I ATCGAT 2 cut(s) 195, 477
BsaBI GATNNNNATC 1 cut(s) 197
BsaHI GRCGYC 2 cut(s) 144, 261
BsaJI CCNNGG 2 cut(s) 384, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 390
Bse1I ACTGG 2 cut(s) 78, 601
Bse8I GATNNNNATC 1 cut(s) 197
BseBI CCWGG 1 cut(s) 203
BseCI ATCGAT 2 cut(s) 195, 477
BseDI CCNNGG 2 cut(s) 384, 573
BseGI GGATG 2 cut(s) 526, 553
BseJI GATNNNNATC 1 cut(s) 197
BseLI CCNNNNNNNGG 1 cut(s) 390
BseMII CTCAG 3 cut(s) 261, 276, 330
BseNI ACTGG 2 cut(s) 78, 601
BseRI GAGGAG 1 cut(s) 64
BseXI GCAGC 2 cut(s) 316, 595
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 2 cut(s) 65, 290
BshNI GGYRCC 1 cut(s) 361
BshVI ATCGAT 2 cut(s) 195, 477
BsiSI CCGG 1 cut(s) 563
BslFI GGGAC 2 cut(s) 23, 365
BslI CCNNNNNNNGG 1 cut(s) 390
BsmAI GTCTC 1 cut(s) 340
BsmFI GGGAC 2 cut(s) 23, 365
BsnI GGCC 2 cut(s) 65, 290
Bsp1407I TGTACA 1 cut(s) 402
Bsp143I GATC 6 cut(s) 192, 210, 474, 478, 548, 565
Bsp19I CCATGG 2 cut(s) 384, 573
BspACI CCGC 2 cut(s) 271, 391
BspANI GGCC 2 cut(s) 65, 290
BspCNI CTCAG 3 cut(s) 260, 277, 329
BspDI ATCGAT 2 cut(s) 195, 477
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 2 cut(s) 363, 567
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 3 cut(s) 556, 560, 573
BspT107I GGYRCC 1 cut(s) 361
BsrGI TGTACA 1 cut(s) 402
BsrI ACTGG 2 cut(s) 78, 601
BssECI CCNNGG 2 cut(s) 384, 573
BssMI GATC 6 cut(s) 192, 210, 474, 478, 548, 565
BssNI GRCGYC 2 cut(s) 144, 261
BssSI CACGAG 1 cut(s) 274
BssT1I CCWWGG 2 cut(s) 384, 573
Bst2BI CACGAG 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 28
Bst6I CTCTTC 1 cut(s) 313
BstACI GRCGYC 2 cut(s) 144, 261
BstAUI TGTACA 1 cut(s) 402
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 3 cut(s) 247, 285, 316
BstDSI CCRYGG 2 cut(s) 384, 573
BstF5I GGATG 2 cut(s) 526, 553
BstFNI CGCG 1 cut(s) 219
BstKTI GATC 6 cut(s) 195, 213, 477, 481, 551, 568
BstMAI GTCTC 1 cut(s) 340
BstMBI GATC 6 cut(s) 192, 210, 474, 478, 548, 565
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 2 cut(s) 201, 561
BstSFI CTRYAG 2 cut(s) 330, 587
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 2 cut(s) 316, 595
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
Bsu15I ATCGAT 2 cut(s) 195, 477
BsuRI GGCC 2 cut(s) 65, 290
BsuTUI ATCGAT 2 cut(s) 195, 477
BtgI CCRYGG 2 cut(s) 384, 573
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 2 cut(s) 526, 553
BtsIMutI CAGTG 2 cut(s) 85, 365
Cac8I GCNNGC 1 cut(s) 412
ClaI ATCGAT 2 cut(s) 195, 477
CseI GACGC 3 cut(s) 113, 208, 225
Csp6I GTAC 3 cut(s) 362, 403, 634
CviAII CATG 4 cut(s) 61, 67, 385, 574
CviJI RGCY 9 cut(s) 65, 77, 206, 270, 290, 329, 355, 410, 586
CviKI_1 RGCY 9 cut(s) 65, 77, 206, 270, 290, 329, 355, 410, 586
CviQI GTAC 3 cut(s) 362, 403, 634
DdeI CTNAG 3 cut(s) 247, 285, 316
DpnI GATC 6 cut(s) 194, 212, 476, 480, 550, 567
DpnII GATC 6 cut(s) 192, 210, 474, 478, 548, 565
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 313
Eco130I CCWWGG 2 cut(s) 384, 573
Eco147I AGGCCT 1 cut(s) 290
Eco32I GATATC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 201
EcoRV GATATC 1 cut(s) 199
EcoT14I CCWWGG 2 cut(s) 384, 573
ErhI CCWWGG 2 cut(s) 384, 573
FaeI CATG 4 cut(s) 64, 70, 388, 577
FaqI GGGAC 2 cut(s) 23, 365
FatI CATG 4 cut(s) 60, 66, 384, 573
FauNDI CATATG 1 cut(s) 462
FbaI TGATCA 1 cut(s) 210
Fnu4HI GCNGC 3 cut(s) 271, 330, 584
FokI GGATG 2 cut(s) 533, 540
Fsp4HI GCNGC 3 cut(s) 271, 330, 584
GluI GCNGC 3 cut(s) 271, 330, 584
HaeIII GGCC 2 cut(s) 65, 290
HapII CCGG 1 cut(s) 563
HgaI GACGC 3 cut(s) 113, 208, 225
Hin1I GRCGYC 2 cut(s) 144, 261
Hin1II CATG 4 cut(s) 64, 70, 388, 577
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 2 cut(s) 243, 664
HpaII CCGG 1 cut(s) 563
HphI GGTGA 2 cut(s) 214, 481
Hpy166II GTNNAC 3 cut(s) 127, 142, 634
Hpy188I TCNGA 5 cut(s) 154, 215, 250, 319, 350
Hpy188III TCNNGA 2 cut(s) 190, 542
Hpy8I GTNNAC 3 cut(s) 127, 142, 634
Hpy99I CGWCG 3 cut(s) 107, 149, 263
HpyAV CCTTC 2 cut(s) 352, 579
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4IV ACGT 4 cut(s) 117, 144, 261, 376
HpyCH4V TGCA 2 cut(s) 177, 332
HpyF3I CTNAG 3 cut(s) 247, 285, 316
HpySE526I ACGT 4 cut(s) 117, 144, 261, 376
Hsp92I GRCGYC 2 cut(s) 144, 261
Hsp92II CATG 4 cut(s) 64, 70, 388, 577
KpnI GGTACC 1 cut(s) 365
Ksp22I TGATCA 1 cut(s) 210
Kzo9I GATC 6 cut(s) 192, 210, 474, 478, 548, 565
LpnPI CCDG 9 cut(s) 91, 144, 171, 188, 215, 241, 424, 576, 582
Lsp1109I GCAGC 2 cut(s) 316, 595
LweI GCATC 1 cut(s) 223
MaeII ACGT 4 cut(s) 117, 144, 261, 376
MaeIII GTNAC 4 cut(s) 80, 113, 220, 280
MalI GATC 6 cut(s) 194, 212, 476, 480, 550, 567
MboI GATC 6 cut(s) 192, 210, 474, 478, 548, 565
MboII GAAGA 3 cut(s) 300, 335, 484
MfeI CAATTG 1 cut(s) 294
MflI RGATCY 1 cut(s) 565
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 1 cut(s) 294
MluI ACGCGT 1 cut(s) 217
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 1 cut(s) 237
MmeI TCCRAC 1 cut(s) 363
MnlI CCTC 7 cut(s) 42, 160, 235, 280, 301, 511, 652
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
MslI CAYNNNNRTG 2 cut(s) 65, 278
Msp20I TGGCCA 1 cut(s) 65
MspI CCGG 1 cut(s) 563
MspR9I CCNGG 2 cut(s) 203, 563
MunI CAATTG 1 cut(s) 294
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 219
NciI CCSGG 1 cut(s) 563
NcoI CCATGG 2 cut(s) 384, 573
NdeI CATATG 1 cut(s) 462
NdeII GATC 6 cut(s) 192, 210, 474, 478, 548, 565
NlaIII CATG 4 cut(s) 64, 70, 388, 577
NlaIV GGNNCC 2 cut(s) 363, 567
NmuCI GTSAC 4 cut(s) 80, 113, 220, 280
OliI CACNNNNGTG 1 cut(s) 278
PceI AGGCCT 1 cut(s) 290
PfeI GAWTC 1 cut(s) 664
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 3 cut(s) 272, 331, 585
PleI GAGTC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 363, 567
PstI CTGCAG 1 cut(s) 334
PsuI RGATCY 1 cut(s) 565
PsyI GACNNNGTC 1 cut(s) 260
RsaI GTAC 3 cut(s) 363, 404, 635
RsaNI GTAC 3 cut(s) 362, 403, 634
RseI CAYNNNNRTG 2 cut(s) 65, 278
SatI GCNGC 3 cut(s) 271, 330, 584
Sau3AI GATC 6 cut(s) 192, 210, 474, 478, 548, 565
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 2 cut(s) 203, 563
SfaNI GCATC 1 cut(s) 223
SfcI CTRYAG 2 cut(s) 330, 587
SmiMI CAYNNNNRTG 2 cut(s) 65, 278
Sse9I AATT 1 cut(s) 294
SseBI AGGCCT 1 cut(s) 290
SsiI CCGC 2 cut(s) 271, 391
StuI AGGCCT 1 cut(s) 290
StyD4I CCNGG 2 cut(s) 201, 561
StyI CCWWGG 2 cut(s) 384, 573
TaaI ACNGT 1 cut(s) 28
TaiI ACGT 4 cut(s) 120, 147, 264, 379
TaqI TCGA 2 cut(s) 195, 477
TasI AATT 1 cut(s) 294
TatI WGTACW 2 cut(s) 402, 633
TauI GCSGC 1 cut(s) 273
TfiI GAWTC 1 cut(s) 664
TscAI CASTG 2 cut(s) 85, 372
TseFI GTSAC 4 cut(s) 80, 113, 220, 280
TseI GCWGC 2 cut(s) 329, 583
Tsp45I GTSAC 4 cut(s) 80, 113, 220, 280
TspDTI ATGAA 1 cut(s) 387
TspRI CASTG 2 cut(s) 85, 372
Tth111I GACNNNGTC 1 cut(s) 260
ZraI GACGTC 2 cut(s) 145, 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.