Rmu_ssc0000422.1_g000037

Leucine Rich Repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000422.1
OG0016779
Physical Location & Seq
Forward (+)
167574 .. 168938
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1365 bp
atgcctgactcagatgatgaggttccactagagatggaaaagaaggagcttttggggatttttgaggtaatgggtgcccttcttgaggactctgattggcctctagagcacccacaaccatgtactgagactcaatggcctggtgttctatgtgagattggggacgaagaccctccaatgtttcacgtcacaaagatacacattggccctgatattctcacccctccttgcaaatcatctgctactttatcagagtcccttctcaaactaccttacttgaaaaccctctcaatatttagttgcttttcaacatcacaagtcactctttctccaaagctctttggtgcattgtcttccttggaacacctagcccttgtgtccaatccaactctctcaggtgaaattccctcaagtttagcccaaatttccagcttaagggttctgactttgtcacagaacaatttacaaggaaaagtccctagtcagattggtgctttggtcagcttagagcaacttgacttgagttacaacaacttgagtggtcaaataccagacgaaattggagggttggagagtttgaccattttggatttaagtcagaatgttcttgaaggtcagctgccctcctcttttggtcagcttcaactcctccaaaagattgatttaagctcaaacaggcttcgaggtatgttgcctccagatatagggaagctcaacaggttggttttgcttgatctgagtcacaacttaataaatgggccaatccctgaaaccctttcaggtttgcagcatttagagtacatggtagctgatagcaacccaatcaagacagaagttcctaactttttagggaagctcatgaatctcaaaacactcagcttttcagaatgtgggttgataggcccattacccaactttctctcttccttgaagaatctcactgctctttgtctaagcaacaatagcctcactgggactgttcgtccaaatttggccactcttccaagtcttgatttactaaatttgagcaacaatcagttaagtggtgagctgacacttccagaagatttcattgagaggcttggaaagaggttggtgctaagaggaaacaatgggctatgtacaagcaatccactcaacaagaaggagaatatttctagccatgtgatagcccctctgtgtttgaatccaagagggcccagagatgaccagaccttggctggtgaacacccaactgagtctgagagagtgaagccattggatcaagaccctgtgcaggaaagttcatctacaggttcttcatggtcacatgtaaaactgaattcatttggttttgttttttgtttgttgagtatgttattgtaa

Protein Analysis

454

Amino Acids

49.36

Weight (kDa)

4.83

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 137 - 222 3.9e-10 Leucine-rich repeat region
LRR_8 PF13855 138 - 179 2.8e-06 Leucine rich repeat
LRR_4 PF12799 144 - 184 8.2e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 169 - 227 8.3e-08 Leucine rich repeat
LRR_8 PF13855 217 - 275 2.6e-07 Leucine rich repeat
LRR_14 PF23598 230 - 316 2.3e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016779)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05310
malus_domestica MD05G1281800.v1.1
prunus_persica Prupe.4G082700_v2.0.a1
pyrus_communis pycom05g26170
rosa_chinensis RchiOBHm_Chr5g0014421
rosa_laevigata RLG00000032107
rosa_multiflora Rmu_ssc0000422.1_g000037
rosa_roxburghii Rroxscaffold_1G00062160
rosa_rugosa Rorug05G0017900
rosa_samantha Rh5AG112800 Rh5BG109600 Rh5CG121400 Rh5DG108600
rosa_wichuraiana Rw5G009790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 74
AccB7I CCANNNNNTGG 1 cut(s) 1216
AclWI GGATC 1 cut(s) 1269
AcoI YGGCCR 1 cut(s) 993
AcsI RAATTY 5 cut(s) 402, 423, 988, 1021, 1321
AfaI GTAC 3 cut(s) 124, 800, 1123
AfiI CCNNNNNNNGG 5 cut(s) 85, 435, 704, 1216, 1276
AflII CTTAAG 1 cut(s) 433
AflIII ACRYGT 1 cut(s) 1309
AgsI TTSAA 6 cut(s) 280, 309, 611, 644, 931, 1186
AhdI GACNNNNNGTC 1 cut(s) 981
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 1 cut(s) 139
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 1 cut(s) 122
AlwI GGATC 1 cut(s) 1269
AoxI GGCC 7 cut(s) 98, 137, 205, 758, 901, 993, 1196
ApaI GGGCCC 1 cut(s) 1200
ApeKI GCWGC 2 cut(s) 619, 787
ApoI RAATTY 5 cut(s) 402, 423, 988, 1021, 1321
AspS9I GGNCC 5 cut(s) 206, 758, 902, 1196, 1197
AsuHPI GGTGA 4 cut(s) 211, 410, 1058, 1235
BaeGI GKGCMC 2 cut(s) 79, 1200
BalI TGGCCA 1 cut(s) 995
BanI GGYRCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 1200
BbsI GAAGAC 2 cut(s) 174, 345
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 606, 799
BccI CCATC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 122
BfaI CTAG 5 cut(s) 29, 104, 368, 480, 1158
BfmI CTRYAG 1 cut(s) 1290
BfrI CTTAAG 1 cut(s) 433
BisI GCNGC 2 cut(s) 620, 788
BlsI GCNGC 2 cut(s) 621, 789
Bme1390I CCNGG 1 cut(s) 141
BmeRI GACNNNNNGTC 1 cut(s) 981
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 5 cut(s) 206, 758, 902, 1196, 1197
BmiI GGNNCC 3 cut(s) 24, 76, 1198
BmrFI CCNGG 1 cut(s) 141
BmrI ACTGGG 1 cut(s) 981
BmuI ACTGGG 1 cut(s) 981
BpiI GAAGAC 2 cut(s) 174, 345
BpmI CTGGAG 1 cut(s) 681
BpuEI CTTGAG 4 cut(s) 104, 394, 541, 556
BsaJI CCNNGG 2 cut(s) 357, 1215
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 435, 704, 1216, 1276
Bse1I ACTGG 1 cut(s) 976
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 357, 1215
BseLI CCNNNNNNNGG 5 cut(s) 85, 435, 704, 1216, 1276
BseMII CTCAG 7 cut(s) 24, 117, 408, 728, 889, 1227, 1233
BseNI ACTGG 1 cut(s) 976
BseRI GAGGAG 2 cut(s) 616, 638
BseSI GKGCMC 2 cut(s) 79, 1200
BseXI GCAGC 2 cut(s) 606, 799
BsgI GTGCAG 1 cut(s) 1295
BshFI GGCC 7 cut(s) 100, 139, 207, 760, 903, 995, 1198
BshNI GGYRCC 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 111
BslFI GGGAC 4 cut(s) 176, 241, 461, 988
BslI CCNNNNNNNGG 5 cut(s) 85, 435, 704, 1216, 1276
BsmAI GTCTC 1 cut(s) 122
BsmFI GGGAC 4 cut(s) 176, 241, 461, 988
BsnI GGCC 7 cut(s) 100, 139, 207, 760, 903, 995, 1198
Bsp120I GGGCCC 1 cut(s) 1196
Bsp1286I GDGCHC 3 cut(s) 79, 111, 1200
Bsp1407I TGTACA 1 cut(s) 1121
Bsp143I GATC 2 cut(s) 733, 1261
BspANI GGCC 7 cut(s) 100, 139, 207, 760, 903, 995, 1198
BspCNI CTCAG 7 cut(s) 23, 118, 407, 729, 888, 1228, 1234
BspHI TCATGA 1 cut(s) 858
BspLI GGNNCC 3 cut(s) 24, 76, 1198
BspPI GGATC 1 cut(s) 1269
BspT107I GGYRCC 1 cut(s) 74
BspTI CTTAAG 1 cut(s) 433
BsrGI TGTACA 1 cut(s) 1121
BsrI ACTGG 1 cut(s) 976
BssECI CCNNGG 2 cut(s) 357, 1215
BssMI GATC 2 cut(s) 733, 1261
BssT1I CCWWGG 2 cut(s) 357, 1215
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 979
Bst6I CTCTTC 2 cut(s) 928, 1005
BstAFI CTTAAG 1 cut(s) 433
BstAUI TGTACA 1 cut(s) 1121
BstENI CCTNNNNNAGG 1 cut(s) 83
BstKTI GATC 2 cut(s) 736, 1264
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 2 cut(s) 733, 1261
BstMWI GCNNNNNNNGC 2 cut(s) 106, 963
BstNI CCWGG 1 cut(s) 141
BstNSI RCATGY 1 cut(s) 1313
BstSCI CCNGG 1 cut(s) 139
BstSFI CTRYAG 1 cut(s) 1290
BstSLI GKGCMC 2 cut(s) 79, 1200
BstV1I GCAGC 2 cut(s) 606, 799
BstV2I GAAGAC 2 cut(s) 174, 345
BsuRI GGCC 7 cut(s) 100, 139, 207, 760, 903, 995, 1198
BtrI CACGTC 1 cut(s) 187
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 2 cut(s) 939, 969
CciI TCATGA 1 cut(s) 858
Cfr13I GGNCC 5 cut(s) 206, 758, 902, 1196, 1197
Csp6I GTAC 3 cut(s) 123, 799, 1122
CspCI CAANNNNNGTGG 2 cut(s) 520, 555
CviAII CATG 6 cut(s) 120, 802, 859, 1163, 1302, 1310
CviQI GTAC 3 cut(s) 123, 799, 1122
DpnI GATC 2 cut(s) 735, 1263
DpnII GATC 2 cut(s) 733, 1261
DriI GACNNNNNGTC 1 cut(s) 981
EaeI YGGCCR 1 cut(s) 993
Eam1104I CTCTTC 2 cut(s) 928, 1005
Eam1105I GACNNNNNGTC 1 cut(s) 981
EarI CTCTTC 2 cut(s) 928, 1005
Eco130I CCWWGG 2 cut(s) 357, 1215
Eco24I GRGCYC 1 cut(s) 1200
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 1196
EcoRI GAATTC 1 cut(s) 1321
EcoRII CCWGG 1 cut(s) 139
EcoT14I CCWWGG 2 cut(s) 357, 1215
EcoT38I GRGCYC 1 cut(s) 1200
ErhI CCWWGG 2 cut(s) 357, 1215
FaeI CATG 6 cut(s) 123, 805, 862, 1166, 1305, 1313
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 4 cut(s) 176, 241, 461, 988
FatI CATG 6 cut(s) 119, 801, 858, 1162, 1301, 1309
Fnu4HI GCNGC 2 cut(s) 620, 788
FriOI GRGCYC 1 cut(s) 1200
Fsp4HI GCNGC 2 cut(s) 620, 788
FspBI CTAG 5 cut(s) 29, 104, 368, 480, 1158
GluI GCNGC 2 cut(s) 620, 788
GsuI CTGGAG 1 cut(s) 681
HaeIII GGCC 7 cut(s) 100, 139, 207, 760, 903, 995, 1198
Hin1II CATG 6 cut(s) 123, 805, 862, 1166, 1305, 1313
HinfI GANTC 9 cut(s) 8, 89, 130, 254, 739, 862, 934, 1186, 1238
HphI GGTGA 4 cut(s) 211, 410, 1058, 1235
Hpy166II GTNNAC 1 cut(s) 1226
Hpy188I TCNGA 9 cut(s) 13, 94, 253, 444, 486, 600, 738, 886, 1243
Hpy188III TCNNGA 9 cut(s) 83, 104, 608, 698, 826, 859, 1010, 1061, 1265
Hpy8I GTNNAC 1 cut(s) 1226
HpyAV CCTTC 5 cut(s) 37, 89, 269, 605, 1138
HpyCH4III ACNGT 1 cut(s) 979
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 4 cut(s) 231, 347, 787, 1276
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 963
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 6 cut(s) 123, 805, 862, 1166, 1305, 1313
Kzo9I GATC 2 cut(s) 733, 1261
LmnI GCTCC 1 cut(s) 46
Lsp1109I GCAGC 2 cut(s) 606, 799
MaeI CTAG 5 cut(s) 29, 104, 368, 480, 1158
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 6 cut(s) 187, 319, 450, 524, 740, 1305
MalI GATC 2 cut(s) 735, 1263
MboI GATC 2 cut(s) 733, 1261
MboII GAAGA 7 cut(s) 179, 345, 915, 943, 992, 1076, 1290
MhlI GDGCHC 3 cut(s) 79, 111, 1200
MlsI TGGCCA 1 cut(s) 995
MluCI AATT 7 cut(s) 402, 423, 460, 558, 988, 1021, 1321
MluNI TGGCCA 1 cut(s) 995
MlyI GAGTC 6 cut(s) 2, 83, 124, 263, 748, 1247
MmeI TCCRAC 2 cut(s) 410, 549
Mox20I TGGCCA 1 cut(s) 995
MscI TGGCCA 1 cut(s) 995
MseI TTAA 5 cut(s) 434, 593, 665, 749, 1040
MslI CAYNNNNRTG 1 cut(s) 118
Msp20I TGGCCA 1 cut(s) 995
MspA1I CMGCKG 1 cut(s) 619
MspCI CTTAAG 1 cut(s) 433
MspR9I CCNGG 1 cut(s) 141
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 2 cut(s) 106, 963
NdeII GATC 2 cut(s) 733, 1261
NlaIII CATG 6 cut(s) 123, 805, 862, 1166, 1305, 1313
NlaIV GGNNCC 3 cut(s) 24, 76, 1198
NmuCI GTSAC 5 cut(s) 187, 319, 450, 740, 1305
NspI RCATGY 1 cut(s) 1313
PagI TCATGA 1 cut(s) 858
PciI ACATGT 1 cut(s) 1309
PfeI GAWTC 3 cut(s) 862, 934, 1186
PflFI GACNNNGTC 1 cut(s) 448
PflMI CCANNNNNTGG 1 cut(s) 1216
PkrI GCNGC 2 cut(s) 621, 789
PleI GAGTC 6 cut(s) 2, 83, 124, 262, 747, 1246
PpsI GAGTC 6 cut(s) 2, 83, 124, 262, 747, 1246
PscI ACATGT 1 cut(s) 1309
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 3 cut(s) 24, 76, 1198
PspOMI GGGCCC 1 cut(s) 1196
PspPI GGNCC 5 cut(s) 206, 758, 902, 1196, 1197
PsyI GACNNNGTC 1 cut(s) 448
PvuII CAGCTG 1 cut(s) 619
RsaI GTAC 3 cut(s) 124, 800, 1123
RsaNI GTAC 3 cut(s) 123, 799, 1122
RseI CAYNNNNRTG 1 cut(s) 118
SaqAI TTAA 5 cut(s) 434, 593, 665, 749, 1040
SatI GCNGC 2 cut(s) 620, 788
Sau3AI GATC 2 cut(s) 733, 1261
Sau96I GGNCC 5 cut(s) 206, 758, 902, 1196, 1197
SchI GAGTC 6 cut(s) 2, 83, 124, 263, 748, 1247
ScrFI CCNGG 1 cut(s) 141
SduI GDGCHC 3 cut(s) 79, 111, 1200
SfcI CTRYAG 1 cut(s) 1290
SmiMI CAYNNNNRTG 1 cut(s) 118
SmlI CTYRAG 5 cut(s) 83, 409, 433, 520, 535
SmoI CTYRAG 5 cut(s) 83, 409, 433, 520, 535
Sse9I AATT 7 cut(s) 402, 423, 460, 558, 988, 1021, 1321
SspI AATATT 2 cut(s) 294, 1153
SspMI CTAG 5 cut(s) 29, 104, 368, 480, 1158
StyD4I CCNGG 1 cut(s) 139
StyI CCWWGG 2 cut(s) 357, 1215
TaaI ACNGT 1 cut(s) 979
TaiI ACGT 1 cut(s) 189
TaqI TCGA 1 cut(s) 682
TasI AATT 7 cut(s) 402, 423, 460, 558, 988, 1021, 1321
TatI WGTACW 3 cut(s) 122, 798, 1121
TfiI GAWTC 3 cut(s) 862, 934, 1186
Tru1I TTAA 5 cut(s) 434, 593, 665, 749, 1040
Tru9I TTAA 5 cut(s) 434, 593, 665, 749, 1040
TscAI CASTG 2 cut(s) 946, 976
TseFI GTSAC 5 cut(s) 187, 319, 450, 740, 1305
TseI GCWGC 2 cut(s) 619, 787
Tsp45I GTSAC 5 cut(s) 187, 319, 450, 740, 1305
TspDTI ATGAA 5 cut(s) 875, 1060, 1275, 1290, 1314
TspRI CASTG 2 cut(s) 946, 976
Tth111I GACNNNGTC 1 cut(s) 448
Van91I CCANNNNNTGG 1 cut(s) 1216
Vha464I CTTAAG 1 cut(s) 433
XagI CCTNNNNNAGG 1 cut(s) 83
XapI RAATTY 5 cut(s) 402, 423, 988, 1021, 1321
XbaI TCTAGA 1 cut(s) 103
XceI RCATGY 1 cut(s) 1313
XcmI CCANNNNNNNNNTGG 1 cut(s) 1217
XspI CTAG 5 cut(s) 29, 104, 368, 480, 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.