Rmu_ssc0000430.1_g000001

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000430.1
OG0016899
Physical Location & Seq
Forward (+)
1 .. 666
666 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 666 bp
gtgccggtgccgcctaagcaagctccggcacagtcagagacgggcagcaactctattttgaaggttgttcacgcaggtggaaatgttgagagttactacatggctattcctgcagccaatattttggagaagtacccttctttcttgctcgctaggcctgaagtcttcaggcggccgtgggactcagtagtgaagccggatgcaatactcacacccggagagaaagtcttccttgttccccgcaggactgtgaggaagctccgtcgcagaatacgaagacccgggaaggatttttcagtcaattgtttcatgtctcaatcgtctattgatgtatcaactgagactctaccacagaggagagatgtcggtagtgcagttggtgatgcaagtgagagtgacgcttctatttgtagtgctattagcagaaacaaaagtgatggcaagaagcatgtgacttttcgtggcatcaagaaacatgtgacatttgctggccttgatgtgaagcacaggccggagaagactcacagcgaaatcgaggagcaagattcgagtgaaagctcgagttctctaaattctctccgagggagaaggagggtacgggttgtgacatggcagccgactcttacagcaataacagaagctcatgggaatgatttgatgaaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

221

Amino Acids

24.53

Weight (kDa)

10.0

Isoelectric Point (pI)

77.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 12 - 213 1.7e-16 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016899)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g40440
malus_domestica MD09G1129900.v1.1 MD17G1118900.v1.1
prunus_persica Prupe.3G195300_v2.0.a1
pyrus_communis pycom09g05300
rosa_chinensis RchiOBHm_Chr2g0155671
rosa_multiflora Rmu_ssc0000430.1_g000001
rosa_roxburghii Rroxscaffold_2G00093570
rosa_rugosa Rorug02G0449000
rosa_samantha Rh2AG515100 Rh2BG526200 Rh2CG500000 Rh2DG535000
rosa_wichuraiana Rw2G042330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 65
Acc36I ACCTGC 1 cut(s) 65
AccB1I GGYRCC 1 cut(s) 7
AciI CCGC 3 cut(s) 11, 172, 241
AcoI YGGCCR 1 cut(s) 173
AcsI RAATTY 1 cut(s) 571
AcuI CTGAAG 2 cut(s) 151, 180
AfaI GTAC 2 cut(s) 134, 597
AflIII ACRYGT 1 cut(s) 475
AgsI TTSAA 1 cut(s) 61
AleI CACNNNNGTG 1 cut(s) 75
AluBI AGCT 4 cut(s) 23, 259, 558, 641
AluI AGCT 4 cut(s) 23, 259, 558, 641
Alw26I GTCTC 3 cut(s) 32, 318, 335
Ama87I CYCGRG 2 cut(s) 281, 559
AoxI GGCC 4 cut(s) 155, 173, 490, 509
ApeKI GCWGC 3 cut(s) 45, 113, 613
ApoI RAATTY 1 cut(s) 571
Asp700I GAANNNNTTC 1 cut(s) 227
AsuC2I CCSGG 3 cut(s) 216, 282, 283
AsuHPI GGTGA 1 cut(s) 392
AvaI CYCGRG 2 cut(s) 281, 559
BanI GGYRCC 1 cut(s) 7
BbsI GAAGAC 4 cut(s) 157, 220, 283, 524
BbvI GCAGC 3 cut(s) 57, 125, 625
BccI CCATC 1 cut(s) 431
BceAI ACGGC 1 cut(s) 160
BcnI CCSGG 3 cut(s) 216, 282, 283
BcoDI GTCTC 3 cut(s) 32, 318, 335
BfaI CTAG 1 cut(s) 153
BfmI CTRYAG 1 cut(s) 111
BfuAI ACCTGC 1 cut(s) 65
BisI GCNGC 5 cut(s) 11, 46, 114, 173, 614
BlsI GCNGC 5 cut(s) 12, 47, 115, 174, 615
Bme1390I CCNGG 3 cut(s) 216, 282, 283
BmeT110I CYCGRG 2 cut(s) 281, 559
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 3 cut(s) 216, 282, 283
BmsI GCATC 3 cut(s) 190, 373, 474
BpiI GAAGAC 4 cut(s) 157, 220, 283, 524
Bpu10I CCTNAGC 1 cut(s) 15
BpuMI CCSGG 3 cut(s) 216, 282, 283
BsaJI CCNNGG 3 cut(s) 176, 281, 580
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bse118I RCCGGY 1 cut(s) 4
BseDI CCNNGG 3 cut(s) 176, 281, 580
BseGI GGATG 1 cut(s) 205
BseMII CTCAG 2 cut(s) 198, 330
BseRI GAGGAG 2 cut(s) 370, 551
BseX3I CGGCCG 1 cut(s) 173
BseXI GCAGC 3 cut(s) 57, 125, 625
BsgI GTGCAG 1 cut(s) 393
Bsh1285I CGRYCG 1 cut(s) 176
BshFI GGCC 4 cut(s) 157, 175, 492, 511
BshNI GGYRCC 1 cut(s) 7
BsiEI CGRYCG 1 cut(s) 176
BsiHKCI CYCGRG 2 cut(s) 281, 559
BsiSI CCGG 6 cut(s) 5, 26, 197, 216, 282, 512
BslFI GGGAC 1 cut(s) 194
BsmAI GTCTC 3 cut(s) 32, 318, 335
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 194
BsnI GGCC 4 cut(s) 157, 175, 492, 511
BsoBI CYCGRG 2 cut(s) 281, 559
BspACI CCGC 3 cut(s) 11, 172, 241
BspANI GGCC 4 cut(s) 157, 175, 492, 511
BspCNI CTCAG 2 cut(s) 197, 331
BspLI GGNNCC 1 cut(s) 9
BspMAI CTGCAG 1 cut(s) 115
BspMI ACCTGC 1 cut(s) 65
BspT107I GGYRCC 1 cut(s) 7
BsrFI RCCGGY 1 cut(s) 4
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 3 cut(s) 176, 281, 580
Bst4CI ACNGT 2 cut(s) 33, 250
BstC8I GCNNGC 3 cut(s) 21, 150, 490
BstDEI CTNAG 3 cut(s) 15, 184, 339
BstDSI CCRYGG 1 cut(s) 176
BstF5I GGATG 1 cut(s) 205
BstMAI GTCTC 3 cut(s) 32, 318, 335
BstMCI CGRYCG 1 cut(s) 176
BstMWI GCNNNNNNNGC 4 cut(s) 10, 16, 110, 154
BstNSI RCATGY 2 cut(s) 452, 479
BstSCI CCNGG 3 cut(s) 214, 280, 281
BstSFI CTRYAG 1 cut(s) 111
BstV1I GCAGC 3 cut(s) 57, 125, 625
BstV2I GAAGAC 4 cut(s) 157, 220, 283, 524
BstXI CCANNNNNNTGG 1 cut(s) 124
BstZI CGGCCG 1 cut(s) 173
BsuRI GGCC 4 cut(s) 157, 175, 492, 511
BtgI CCRYGG 1 cut(s) 176
BtsCI GGATG 1 cut(s) 205
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 3 cut(s) 21, 150, 490
Cfr10I RCCGGY 1 cut(s) 4
Cfr9I CCCGGG 1 cut(s) 281
CseI GACGC 1 cut(s) 407
Csp6I GTAC 2 cut(s) 133, 596
CviAII CATG 6 cut(s) 100, 310, 449, 476, 609, 644
CviQI GTAC 2 cut(s) 133, 596
DdeI CTNAG 3 cut(s) 15, 184, 339
EaeI YGGCCR 1 cut(s) 173
EagI CGGCCG 1 cut(s) 173
EclXI CGGCCG 1 cut(s) 173
Eco147I AGGCCT 1 cut(s) 157
Eco52I CGGCCG 1 cut(s) 173
Eco57I CTGAAG 2 cut(s) 151, 180
Eco88I CYCGRG 2 cut(s) 281, 559
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 6 cut(s) 103, 313, 452, 479, 612, 647
FaiI YATR 6 cut(s) 101, 311, 450, 477, 610, 645
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FaqI GGGAC 1 cut(s) 194
FatI CATG 6 cut(s) 99, 309, 448, 475, 608, 643
FauI CCCGC 1 cut(s) 248
Fnu4HI GCNGC 5 cut(s) 11, 46, 114, 173, 614
FokI GGATG 1 cut(s) 212
Fsp4HI GCNGC 5 cut(s) 11, 46, 114, 173, 614
FspBI CTAG 1 cut(s) 153
GluI GCNGC 5 cut(s) 11, 46, 114, 173, 614
HaeIII GGCC 4 cut(s) 157, 175, 492, 511
HapII CCGG 6 cut(s) 5, 26, 197, 216, 282, 512
HgaI GACGC 1 cut(s) 407
Hin1II CATG 6 cut(s) 103, 313, 452, 479, 612, 647
HinfI GANTC 5 cut(s) 182, 343, 520, 545, 619
HpaII CCGG 6 cut(s) 5, 26, 197, 216, 282, 512
HphI GGTGA 1 cut(s) 392
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 2 cut(s) 37, 581
Hpy188III TCNNGA 1 cut(s) 469
Hpy8I GTNNAC 1 cut(s) 70
Hpy99I CGWCG 1 cut(s) 267
HpyAV CCTTC 4 cut(s) 55, 147, 280, 582
HpyCH4III ACNGT 2 cut(s) 33, 250
HpyCH4V TGCA 4 cut(s) 113, 203, 374, 386
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 16, 110, 154
HpyF3I CTNAG 3 cut(s) 15, 184, 339
Hsp92II CATG 6 cut(s) 103, 313, 452, 479, 612, 647
LmnI GCTCC 3 cut(s) 28, 264, 538
Lsp1109I GCAGC 3 cut(s) 57, 125, 625
LweI GCATC 3 cut(s) 190, 373, 474
MaeI CTAG 1 cut(s) 153
MaeIII GTNAC 5 cut(s) 92, 395, 451, 478, 604
MboII GAAGA 4 cut(s) 157, 220, 288, 529
MfeI CAATTG 1 cut(s) 301
MluCI AATT 2 cut(s) 301, 571
MlyI GAGTC 4 cut(s) 176, 337, 514, 613
MnlI CCTC 5 cut(s) 246, 348, 529, 575, 585
MroXI GAANNNNTTC 1 cut(s) 227
MslI CAYNNNNRTG 2 cut(s) 75, 648
MspI CCGG 6 cut(s) 5, 26, 197, 216, 282, 512
MspR9I CCNGG 3 cut(s) 216, 282, 283
MunI CAATTG 1 cut(s) 301
MwoI GCNNNNNNNGC 4 cut(s) 10, 16, 110, 154
NciI CCSGG 3 cut(s) 216, 282, 283
NlaIII CATG 6 cut(s) 103, 313, 452, 479, 612, 647
NlaIV GGNNCC 1 cut(s) 9
NmuCI GTSAC 4 cut(s) 395, 451, 478, 604
NspI RCATGY 2 cut(s) 452, 479
OliI CACNNNNGTG 1 cut(s) 75
PaeR7I CTCGAG 1 cut(s) 559
PaqCI CACCTGC 1 cut(s) 65
PceI AGGCCT 1 cut(s) 157
PciI ACATGT 1 cut(s) 475
PcsI WCGNNNNNNNCGW 1 cut(s) 271
PdmI GAANNNNTTC 1 cut(s) 227
PfeI GAWTC 1 cut(s) 545
PkrI GCNGC 5 cut(s) 12, 47, 115, 174, 615
PleI GAGTC 4 cut(s) 176, 337, 514, 613
PpsI GAGTC 4 cut(s) 176, 337, 514, 613
PscI ACATGT 1 cut(s) 475
PspN4I GGNNCC 1 cut(s) 9
PspXI VCTCGAGB 1 cut(s) 559
PstI CTGCAG 1 cut(s) 115
RsaI GTAC 2 cut(s) 134, 597
RsaNI GTAC 2 cut(s) 133, 596
RseI CAYNNNNRTG 2 cut(s) 75, 648
SatI GCNGC 5 cut(s) 11, 46, 114, 173, 614
SchI GAGTC 4 cut(s) 176, 337, 514, 613
ScrFI CCNGG 3 cut(s) 216, 282, 283
SetI ASST 6 cut(s) 25, 66, 79, 261, 560, 643
SfaNI GCATC 3 cut(s) 190, 373, 474
SfcI CTRYAG 1 cut(s) 111
Sfr274I CTCGAG 1 cut(s) 559
SlaI CTCGAG 1 cut(s) 559
SmaI CCCGGG 1 cut(s) 283
SmiMI CAYNNNNRTG 2 cut(s) 75, 648
SmlI CTYRAG 1 cut(s) 559
SmoI CTYRAG 1 cut(s) 559
Sse9I AATT 2 cut(s) 301, 571
SseBI AGGCCT 1 cut(s) 157
SsiI CCGC 3 cut(s) 11, 172, 241
SspI AATATT 1 cut(s) 121
SspMI CTAG 1 cut(s) 153
StuI AGGCCT 1 cut(s) 157
StyD4I CCNGG 3 cut(s) 214, 280, 281
TaaI ACNGT 2 cut(s) 33, 250
TaqI TCGA 3 cut(s) 534, 548, 560
TasI AATT 2 cut(s) 301, 571
TauI GCSGC 2 cut(s) 13, 175
TfiI GAWTC 1 cut(s) 545
TseFI GTSAC 4 cut(s) 395, 451, 478, 604
TseI GCWGC 3 cut(s) 45, 113, 613
Tsp45I GTSAC 4 cut(s) 395, 451, 478, 604
TspDTI ATGAA 1 cut(s) 298
TspGWI ACGGA 1 cut(s) 251
TspMI CCCGGG 1 cut(s) 281
XapI RAATTY 1 cut(s) 571
XceI RCATGY 2 cut(s) 452, 479
XhoI CTCGAG 1 cut(s) 559
XmaI CCCGGG 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 227
XspI CTAG 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.