Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_ssc0000432.1

Orthogroup ID

OG0005676

Physical Location & Seq

Genomic Coordinates Forward (+)

273110 .. 277354

Gene Structure 4245 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

atgagcccatcaaagaacactaggaagctggaggtggcttcaccagtcccagctgacatagacattgccaattcagttgagcctgttcacatctctgagattgccaaagaccttaatctcagtcctgaccactatgatctctatggaaaatacaaggccaaggttttgttatctgtgattgatgaactgaaaggatctagtgatgggtattatgttgttgtgggagggattactcctacacctcttggagaaggcaagtctaccaccactgttggactgtgtcaagctttgggtgcttttcaagataagaaggttgtcacttgccttcgtcaaccatcacaaggaccaacttttggaattaaaggtggtgcagcaggtggtggttacagtcaagtgatcccaatggatgagtttaatctgcacttaacaggagatattcacgcaataacagctgcaaacaatcttctagctgctgccattgatactcgaatgttccatgaagcaacccaatcagataaggctctaatgaaccgattatgcccaccaaacaaagaaggaaaaaagagttttaatgatatcatgtttagacgtctaaagaagctgggtatctccaagacgcagcctgaggaccttacaccggaagaagttaagaaatttgctaggcttgatattgatccagattctatcacctggagaagagtaatggatataaatgaccggctgttgagaaaaataacagttggccagggccctgaagagaaagggatggtgagagaaacaggatttgatatttcagttgccagtgagataatggcagttttggcccttacgacatctctagcagatatgagggagaggcttggcaaaatggtaatcggaaatagcagggctggtgacccagttactgccgacgatcttggtgtcggaggtgcattaacggttctaatgaaggatgctattaaccctacattaatgcagactcttgaggggaccccagttcttgttcatgctggtccttttgcaaatattgcgcatggaaattcctccattgtggctgataagattgcgctaaagctggtgggaccaggtgggtttgtagtcacagaagctggttttggtgctgatattggcactgagaaatttatgaatataaaatgccggtatagtggtttaacaccgcagtgcgcaattatagttgccaccataagggccttgaaaatgcatggtggtggcccggatgttgttgctgggaagcctcttaatagtgcctacataactgagaatgtggctctggttgaggctggctgtgtgaatttggccaggcatattacaaatacaaaagcctatggtgtgaatgttgttgttgccgtgaacaagttctcaactgactctgatgcagaattgaatgcagttcgaaatgcagcactggctgctggggcgtatgatgctgtgatatgtactcaccatgcccatggtggaaaaggagcggtagacctagggattgcggttcagaaagcctgtgagaatgttacacaaccattaaagtttttatatcctttggacttcagtattaaagaaaagatagaggcaatagccaggtcatatggtgcaagtggtgttgaatactcggagcaggctgaaaagcagattgcaatgtacagcaggcaagggttttctggtctgccaatatgcatggcaaagacccagtattcattttctgataaagctactgcgaaaggagcaccatccaattttgtcttaccgataagggatgtgagggcaagcattggagctggatttatatatcctttggtaggaaccatgagcacaatgccaggacttccaactaggccttgcttttatgatattgatcttgacaccaccactggaagggttattggtctttcttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004329 GO:0004477 GO:0004488 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006144 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009112 GO:0009113 GO:0009256 GO:0009257 GO:0009396 GO:0009507 GO:0009536 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0016874 GO:0016879 GO:0018130 GO:0019238 GO:0019438 GO:0019752 GO:0034641 GO:0042221 GO:0042398 GO:0042440 GO:0042558 GO:0042559 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046112 GO:0046148 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00670 ko00720 ko01100 ko01120 ko01200 map00670 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

FTHFS

Protein Analysis

636

Amino Acids

67.65

Weight (kDa)

7.16

Isoelectric Point (pI)

32.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FTHFS	PF01268	18 - 636	2.7e-252	Formate--tetrahydrofolate ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005676)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50480
fragaria_vesca	FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470
malus_domestica	MD03G1012400.v1.1 MD11G1015100.v1.1
prunus_persica	Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1
pyrus_communis	pycom03g01100 pycom11g01040
rosa_chinensis	RchiOBHm_Chr5g0081161
rosa_laevigata	RLG00000036914
rosa_multiflora	Rmu_sc0016124.1_g000001 Rmu_ssc0000432.1_g000078
rosa_roxburghii	Rroxscaffold_1G00002300
rosa_rugosa	Rorug05G0473600
rosa_samantha	Rh1DG124300 Rh5AG526200 Rh5BG550500 Rh5CG573400 Rh5DG560200
rosa_wichuraiana	Rw1G009600 Rw5G048890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	365
AatII	GACGTC	1 cut(s)	592
Acc16I	TGCGCA	2 cut(s)	1032, 1186
Acc36I	ACCTGC	1 cut(s)	365
AccB7I	CCANNNNNTGG	1 cut(s)	353
AccBSI	CCGCTC	1 cut(s)	1487
AccI	GTMKAC	2 cut(s)	260, 1491
AciI	CCGC	3 cut(s)	1178, 1487, 1505
AclWI	GGATC	3 cut(s)	202, 391, 668
AcoI	YGGCCR	2 cut(s)	742, 1317
AcsI	RAATTY	4 cut(s)	653, 1039, 1139, 1312
AcuI	CTGAAG	2 cut(s)	774, 1549
AcyI	GRCGYC	1 cut(s)	589
AfaI	GTAC	2 cut(s)	1459, 1658
AfiI	CCNNNNNNNGG	6 cut(s)	341, 353, 637, 1206, 1814, 1890
AgsI	TTSAA	4 cut(s)	302, 1216, 1405, 1622
AjnI	CCWGG	6 cut(s)	689, 744, 1084, 1319, 1595, 1834
AjuI	GAANNNNNNNTTGG	2 cut(s)	1098, 1130
AleI	CACNNNNGTG	1 cut(s)	1180
Alw21I	GWGCWC	2 cut(s)	1744, 1829
AlwI	GGATC	3 cut(s)	202, 391, 668
AlwNI	CAGNNNCTG	2 cut(s)	905, 1109
AoxI	GGCC	8 cut(s)	156, 742, 748, 822, 1209, 1231, 1317, 1850
ApaI	GGGCCC	1 cut(s)	752
ApeKI	GCWGC	7 cut(s)	371, 452, 470, 473, 619, 1421, 1430
ApoI	RAATTY	4 cut(s)	653, 1039, 1139, 1312
AseI	ATTAAT	1 cut(s)	971
Asp700I	GAANNNNTTC	1 cut(s)	1376
AspA2I	CCTAGG	1 cut(s)	1495
AspLEI	GCGC	3 cut(s)	1033, 1069, 1187
AsuC2I	CCSGG	1 cut(s)	1235
AsuHPI	GGTGA	5 cut(s)	33, 679, 781, 905, 1454
AsuII	TTCGAA	1 cut(s)	1414
AvaII	GGWCC	5 cut(s)	344, 628, 990, 1013, 1082
AvrII	CCTAGG	1 cut(s)	1495
AxyI	CCTNAGG	1 cut(s)	624
BaeGI	GKGCMC	1 cut(s)	752
BalI	TGGCCA	2 cut(s)	744, 1319
BanII	GRGCYC	2 cut(s)	8, 752
Bbv12I	GWGCWC	2 cut(s)	1744, 1829
BbvI	GCAGC	7 cut(s)	383, 439, 457, 460, 631, 1417, 1433
BccI	CCATC	5 cut(s)	16, 197, 343, 760, 1753
BceAI	ACGGC	1 cut(s)	1352
BciT130I	CCWGG	6 cut(s)	691, 746, 1086, 1321, 1597, 1836
BcnI	CCSGG	1 cut(s)	1235
BfaI	CTAG	7 cut(s)	21, 198, 467, 660, 839, 1496, 1848
BfuAI	ACCTGC	1 cut(s)	365
BisI	GCNGC	7 cut(s)	372, 453, 471, 474, 620, 1422, 1431
BlnI	CCTAGG	1 cut(s)	1495
BlsI	GCNGC	7 cut(s)	373, 454, 472, 475, 621, 1423, 1432
Bme1390I	CCNGG	7 cut(s)	691, 746, 1086, 1235, 1321, 1597, 1836
Bme18I	GGWCC	5 cut(s)	344, 628, 990, 1013, 1082
BmiI	GGNNCC	5 cut(s)	750, 991, 992, 1083, 1819
BmrFI	CCNGG	7 cut(s)	691, 746, 1086, 1235, 1321, 1597, 1836
BmrI	ACTGGG	3 cut(s)	893, 989, 1699
BmsI	GCATC	3 cut(s)	943, 1384, 1435
BmuI	ACTGGG	3 cut(s)	893, 989, 1699
BpmI	CTGGAG	2 cut(s)	50, 712
Bpu14I	TTCGAA	1 cut(s)	1414
BpuEI	CTTGAG	1 cut(s)	1004
BpuMI	CCSGG	1 cut(s)	1235
BsaBI	GATNNNNATC	2 cut(s)	672, 1869
BsaHI	GRCGYC	1 cut(s)	589
BsaJI	CCNNGG	4 cut(s)	159, 745, 1471, 1495
BsaWI	WCCGGW	1 cut(s)	637
Bsc4I	CCNNNNNNNGG	6 cut(s)	341, 353, 637, 1206, 1814, 1890
Bse118I	RCCGGY	2 cut(s)	717, 1158
Bse1I	ACTGG	7 cut(s)	44, 801, 899, 995, 1431, 1705, 1891
Bse21I	CCTNAGG	1 cut(s)	624
Bse3DI	GCAATG	2 cut(s)	63, 1659
Bse8I	GATNNNNATC	2 cut(s)	672, 1869
BseBI	CCWGG	6 cut(s)	691, 746, 1086, 1321, 1597, 1836
BseDI	CCNNGG	4 cut(s)	159, 745, 1471, 1495
BseGI	GGATG	6 cut(s)	412, 771, 958, 1243, 1745, 1777
BseJI	GATNNNNATC	2 cut(s)	672, 1869
BseLI	CCNNNNNNNGG	6 cut(s)	341, 353, 637, 1206, 1814, 1890
BseMI	GCAATG	2 cut(s)	63, 1659
BseMII	CTCAG	5 cut(s)	87, 133, 615, 1125, 1269
BseNI	ACTGG	7 cut(s)	44, 801, 899, 995, 1431, 1705, 1891
BseSI	GKGCMC	1 cut(s)	752
BseXI	GCAGC	7 cut(s)	383, 439, 457, 460, 631, 1417, 1433
BseYI	CCCAGC	4 cut(s)	49, 601, 1247, 1433
BsgI	GTGCAG	2 cut(s)	390, 404
BshFI	GGCC	8 cut(s)	158, 744, 750, 824, 1211, 1233, 1319, 1852
BsiHKAI	GWGCWC	2 cut(s)	1744, 1829
BsiSI	CCGG	4 cut(s)	638, 718, 1159, 1235
BslFI	GGGAC	3 cut(s)	32, 1003, 1095
BslI	CCNNNNNNNGG	6 cut(s)	341, 353, 637, 1206, 1814, 1890
BsmFI	GGGAC	3 cut(s)	32, 1003, 1095
BsmI	GAATGC	1 cut(s)	1411
BsnI	GGCC	8 cut(s)	158, 744, 750, 824, 1211, 1233, 1319, 1852
Bsp119I	TTCGAA	1 cut(s)	1414
Bsp120I	GGGCCC	1 cut(s)	748
Bsp1286I	GDGCHC	4 cut(s)	8, 752, 1744, 1829
Bsp1407I	TGTACA	1 cut(s)	1656
Bsp143I	GATC	6 cut(s)	136, 194, 396, 673, 913, 1870
Bsp19I	CCATGG	1 cut(s)	1471
BspACI	CCGC	3 cut(s)	1178, 1487, 1505
BspANI	GGCC	8 cut(s)	158, 744, 750, 824, 1211, 1233, 1319, 1852
BspCNI	CTCAG	5 cut(s)	88, 132, 616, 1126, 1270
BspLI	GGNNCC	5 cut(s)	750, 991, 992, 1083, 1819
BspMI	ACCTGC	1 cut(s)	365
BspPI	GGATC	3 cut(s)	202, 391, 668
BspT104I	TTCGAA	1 cut(s)	1414
BsrBI	CCGCTC	1 cut(s)	1487
BsrDI	GCAATG	2 cut(s)	63, 1659
BsrFI	RCCGGY	2 cut(s)	717, 1158
BsrGI	TGTACA	1 cut(s)	1656
BsrI	ACTGG	7 cut(s)	44, 801, 899, 995, 1431, 1705, 1891
BssAI	RCCGGY	2 cut(s)	717, 1158
BssECI	CCNNGG	4 cut(s)	159, 745, 1471, 1495
BssMI	GATC	6 cut(s)	136, 194, 396, 673, 913, 1870
BssNI	GRCGYC	1 cut(s)	589
BssT1I	CCWWGG	3 cut(s)	159, 1471, 1495
Bst2UI	CCWGG	6 cut(s)	691, 746, 1086, 1321, 1597, 1836
Bst4CI	ACNGT	5 cut(s)	271, 279, 389, 739, 940
Bst6I	CTCTTC	2 cut(s)	691, 750
BstACI	GRCGYC	1 cut(s)	589
BstAPI	GCANNNNNTGC	2 cut(s)	1028, 1430
BstAUI	TGTACA	1 cut(s)	1656
BstBI	TTCGAA	1 cut(s)	1414
BstC8I	GCNNGC	4 cut(s)	1303, 1635, 1664, 1783
BstDEI	CTNAG	5 cut(s)	96, 119, 624, 1134, 1278
BstDSI	CCRYGG	1 cut(s)	1471
BstEII	GGTNACC	1 cut(s)	893
BstENI	CCTNNNNNAGG	1 cut(s)	1812
BstF5I	GGATG	6 cut(s)	412, 771, 958, 1243, 1745, 1777
BstHHI	GCGC	3 cut(s)	1033, 1069, 1187
BstKTI	GATC	6 cut(s)	139, 197, 399, 676, 916, 1873
BstMBI	GATC	6 cut(s)	136, 194, 396, 673, 913, 1870
BstMWI	GCNNNNNNNGC	9 cut(s)	293, 449, 821, 1028, 1427, 1430, 1436, 1445, 1739
BstNI	CCWGG	6 cut(s)	691, 746, 1086, 1321, 1597, 1836
BstPI	GGTNACC	1 cut(s)	893
BstSCI	CCNGG	7 cut(s)	689, 744, 1084, 1233, 1319, 1595, 1834
BstSLI	GKGCMC	1 cut(s)	752
BstV1I	GCAGC	7 cut(s)	383, 439, 457, 460, 631, 1417, 1433
BstX2I	RGATCY	1 cut(s)	194
BstXI	CCANNNNNNTGG	1 cut(s)	1472
BstYI	RGATCY	1 cut(s)	194
Bsu36I	CCTNAGG	1 cut(s)	624
BsuRI	GGCC	8 cut(s)	158, 744, 750, 824, 1211, 1233, 1319, 1852
BtgI	CCRYGG	1 cut(s)	1471
BtsCI	GGATG	6 cut(s)	412, 771, 958, 1243, 1745, 1777
BtsI	GCAGTG	1 cut(s)	1187
BtsIMutI	CAGTG	6 cut(s)	267, 808, 1131, 1187, 1424, 1884
BveI	ACCTGC	1 cut(s)	365
Cac8I	GCNNGC	4 cut(s)	1303, 1635, 1664, 1783
CaiI	CAGNNNCTG	2 cut(s)	905, 1109
CfoI	GCGC	3 cut(s)	1033, 1069, 1187
Cfr10I	RCCGGY	2 cut(s)	717, 1158
CseI	GACGC	1 cut(s)	625
CsiI	ACCWGGT	1 cut(s)	1084
Csp6I	GTAC	2 cut(s)	1458, 1657
CspCI	CAANNNNNGTGG	2 cut(s)	253, 288
CviAII	CATG	9 cut(s)	497, 580, 1007, 1034, 1223, 1466, 1472, 1693, 1822
CviQI	GTAC	2 cut(s)	1458, 1657
DdeI	CTNAG	5 cut(s)	96, 119, 624, 1134, 1278
DpnI	GATC	6 cut(s)	138, 196, 398, 675, 915, 1872
DpnII	GATC	6 cut(s)	136, 194, 396, 673, 913, 1870
EaeI	YGGCCR	2 cut(s)	742, 1317
Eam1104I	CTCTTC	2 cut(s)	691, 750
EarI	CTCTTC	2 cut(s)	691, 750
Eco130I	CCWWGG	3 cut(s)	159, 1471, 1495
Eco147I	AGGCCT	1 cut(s)	1852
Eco24I	GRGCYC	2 cut(s)	8, 752
Eco32I	GATATC	1 cut(s)	577
Eco47I	GGWCC	5 cut(s)	344, 628, 990, 1013, 1082
Eco57I	CTGAAG	2 cut(s)	774, 1549
Eco81I	CCTNAGG	1 cut(s)	624
Eco91I	GGTNACC	1 cut(s)	893
EcoNI	CCTNNNNNAGG	1 cut(s)	1812
EcoO109I	RGGNCCY	5 cut(s)	628, 748, 749, 990, 1209
EcoO65I	GGTNACC	1 cut(s)	893
EcoRII	CCWGG	6 cut(s)	689, 744, 1084, 1319, 1595, 1834
EcoRV	GATATC	1 cut(s)	577
EcoT14I	CCWWGG	3 cut(s)	159, 1471, 1495
EcoT22I	ATGCAT	2 cut(s)	1224, 1694
EcoT38I	GRGCYC	2 cut(s)	8, 752
ErhI	CCWWGG	3 cut(s)	159, 1471, 1495
FaeI	CATG	9 cut(s)	500, 583, 1010, 1037, 1226, 1469, 1475, 1696, 1825
FaqI	GGGAC	3 cut(s)	32, 1003, 1095
FatI	CATG	9 cut(s)	496, 579, 1006, 1033, 1222, 1465, 1471, 1692, 1821
FauNDI	CATATG	1 cut(s)	1603
FblI	GTMKAC	2 cut(s)	260, 1491
Fnu4HI	GCNGC	7 cut(s)	372, 453, 471, 474, 620, 1422, 1431
FokI	GGATG	6 cut(s)	419, 778, 965, 1250, 1732, 1784
FriOI	GRGCYC	2 cut(s)	8, 752
Fsp4HI	GCNGC	7 cut(s)	372, 453, 471, 474, 620, 1422, 1431
FspBI	CTAG	7 cut(s)	21, 198, 467, 660, 839, 1496, 1848
FspI	TGCGCA	2 cut(s)	1032, 1186
GlaI	GCGC	3 cut(s)	1032, 1068, 1186
GluI	GCNGC	7 cut(s)	372, 453, 471, 474, 620, 1422, 1431
GsaI	CCCAGC	4 cut(s)	53, 605, 1251, 1437
GsuI	CTGGAG	2 cut(s)	50, 712
HaeIII	GGCC	8 cut(s)	158, 744, 750, 824, 1211, 1233, 1319, 1852
HapII	CCGG	4 cut(s)	638, 718, 1159, 1235
HgaI	GACGC	1 cut(s)	625
HhaI	GCGC	3 cut(s)	1033, 1069, 1187
Hin1I	GRCGYC	1 cut(s)	589
Hin1II	CATG	9 cut(s)	500, 583, 1010, 1037, 1226, 1469, 1475, 1696, 1825
Hin6I	GCGC	3 cut(s)	1031, 1067, 1185
HinP1I	GCGC	3 cut(s)	1031, 1067, 1185
HincII	GTYRAC	1 cut(s)	332
HindII	GTYRAC	1 cut(s)	332
HindIII	AAGCTT	1 cut(s)	285
HinfI	GANTC	3 cut(s)	680, 979, 1388
HpaII	CCGG	4 cut(s)	638, 718, 1159, 1235
HphI	GGTGA	5 cut(s)	33, 679, 781, 905, 1454
Hpy166II	GTNNAC	5 cut(s)	88, 261, 332, 1372, 1492
Hpy188I	TCNGA	8 cut(s)	97, 514, 878, 926, 1393, 1512, 1630, 1720
Hpy188III	TCNNGA	6 cut(s)	125, 302, 677, 983, 1874, 1908
Hpy8I	GTNNAC	5 cut(s)	88, 261, 332, 1372, 1492
Hpy99I	CGWCG	1 cut(s)	914
HpyAV	CCTTC	6 cut(s)	245, 304, 335, 548, 943, 1884
HpyCH4III	ACNGT	5 cut(s)	271, 279, 389, 739, 940
HpyCH4IV	ACGT	1 cut(s)	589
HpyF10VI	GCNNNNNNNGC	9 cut(s)	293, 449, 821, 1028, 1427, 1430, 1436, 1445, 1739
HpyF3I	CTNAG	5 cut(s)	96, 119, 624, 1134, 1278
HpySE526I	ACGT	1 cut(s)	589
Hsp92I	GRCGYC	1 cut(s)	589
Hsp92II	CATG	9 cut(s)	500, 583, 1010, 1037, 1226, 1469, 1475, 1696, 1825
HspAI	GCGC	3 cut(s)	1031, 1067, 1185
KflI	GGGWCCC	1 cut(s)	990
Kzo9I	GATC	6 cut(s)	136, 194, 396, 673, 913, 1870
LmnI	GCTCC	4 cut(s)	1484, 1630, 1739, 1790
Lsp1109I	GCAGC	7 cut(s)	383, 439, 457, 460, 631, 1417, 1433
LweI	GCATC	3 cut(s)	943, 1384, 1435
MabI	ACCWGGT	1 cut(s)	1084
MaeI	CTAG	7 cut(s)	21, 198, 467, 660, 839, 1496, 1848
MaeII	ACGT	1 cut(s)	589
MaeIII	GTNAC	6 cut(s)	316, 383, 893, 901, 1099, 1528
MalI	GATC	6 cut(s)	138, 196, 398, 675, 915, 1872
MbiI	CCGCTC	1 cut(s)	1487
MboI	GATC	6 cut(s)	136, 194, 396, 673, 913, 1870
MboII	GAAGA	4 cut(s)	455, 653, 708, 767
MflI	RGATCY	1 cut(s)	194
MhlI	GDGCHC	4 cut(s)	8, 752, 1744, 1829
MlsI	TGGCCA	2 cut(s)	744, 1319
MluCI	AATT	9 cut(s)	70, 357, 653, 1039, 1139, 1188, 1312, 1400, 1750
MluNI	TGGCCA	2 cut(s)	744, 1319
MlyI	GAGTC	2 cut(s)	973, 1382
MmeI	TCCRAC	3 cut(s)	253, 904, 1868
Mox20I	TGGCCA	2 cut(s)	744, 1319
Mph1103I	ATGCAT	2 cut(s)	1224, 1694
MroXI	GAANNNNTTC	1 cut(s)	1376
MscI	TGGCCA	2 cut(s)	744, 1319
MslI	CAYNNNNRTG	3 cut(s)	1180, 1227, 1470
Msp20I	TGGCCA	2 cut(s)	744, 1319
MspA1I	CMGCKG	2 cut(s)	53, 452
MspI	CCGG	4 cut(s)	638, 718, 1159, 1235
MspR9I	CCNGG	7 cut(s)	691, 746, 1086, 1235, 1321, 1597, 1836
Mva1269I	GAATGC	1 cut(s)	1411
MvaI	CCWGG	6 cut(s)	691, 746, 1086, 1321, 1597, 1836
MwoI	GCNNNNNNNGC	9 cut(s)	293, 449, 821, 1028, 1427, 1430, 1436, 1445, 1739
NciI	CCSGG	1 cut(s)	1235
NcoI	CCATGG	1 cut(s)	1471
NdeI	CATATG	1 cut(s)	1603
NdeII	GATC	6 cut(s)	136, 194, 396, 673, 913, 1870
NlaIII	CATG	9 cut(s)	500, 583, 1010, 1037, 1226, 1469, 1475, 1696, 1825
NlaIV	GGNNCC	5 cut(s)	750, 991, 992, 1083, 1819
NmuCI	GTSAC	3 cut(s)	316, 893, 1099
NsbI	TGCGCA	2 cut(s)	1032, 1186
NsiI	ATGCAT	2 cut(s)	1224, 1694
NspV	TTCGAA	1 cut(s)	1414
OliI	CACNNNNGTG	1 cut(s)	1180
PaqCI	CACCTGC	1 cut(s)	365
PceI	AGGCCT	1 cut(s)	1852
PctI	GAATGC	1 cut(s)	1411
PdmI	GAANNNNTTC	1 cut(s)	1376
PfeI	GAWTC	1 cut(s)	680
PflFI	GACNNNGTC	1 cut(s)	279
PflMI	CCANNNNNTGG	1 cut(s)	353
PkrI	GCNGC	7 cut(s)	373, 454, 472, 475, 621, 1423, 1432
PleI	GAGTC	2 cut(s)	973, 1382
PpsI	GAGTC	2 cut(s)	973, 1382
PpuMI	RGGWCCY	2 cut(s)	628, 990
PshBI	ATTAAT	1 cut(s)	971
Psp5II	RGGWCCY	2 cut(s)	628, 990
Psp6I	CCWGG	6 cut(s)	689, 744, 1084, 1319, 1595, 1834
PspEI	GGTNACC	1 cut(s)	893
PspFI	CCCAGC	4 cut(s)	49, 601, 1247, 1433
PspGI	CCWGG	6 cut(s)	689, 744, 1084, 1319, 1595, 1834
PspN4I	GGNNCC	5 cut(s)	750, 991, 992, 1083, 1819
PspOMI	GGGCCC	1 cut(s)	748
PspPPI	RGGWCCY	2 cut(s)	628, 990
PstNI	CAGNNNCTG	2 cut(s)	905, 1109
PsuI	RGATCY	1 cut(s)	194
PsyI	GACNNNGTC	1 cut(s)	279
PvuII	CAGCTG	2 cut(s)	53, 452
RsaI	GTAC	2 cut(s)	1459, 1658
RsaNI	GTAC	2 cut(s)	1458, 1657
RseI	CAYNNNNRTG	3 cut(s)	1180, 1227, 1470
SatI	GCNGC	7 cut(s)	372, 453, 471, 474, 620, 1422, 1431
Sau3AI	GATC	6 cut(s)	136, 194, 396, 673, 913, 1870
SchI	GAGTC	2 cut(s)	973, 1382
ScrFI	CCNGG	7 cut(s)	691, 746, 1086, 1235, 1321, 1597, 1836
SduI	GDGCHC	4 cut(s)	8, 752, 1744, 1829
SexAI	ACCWGGT	1 cut(s)	1084
SfaNI	GCATC	3 cut(s)	943, 1384, 1435
SfuI	TTCGAA	1 cut(s)	1414
SinI	GGWCC	5 cut(s)	344, 628, 990, 1013, 1082
SmiMI	CAYNNNNRTG	3 cut(s)	1180, 1227, 1470
SmlI	CTYRAG	1 cut(s)	983
SmoI	CTYRAG	1 cut(s)	983
Sse9I	AATT	9 cut(s)	70, 357, 653, 1039, 1139, 1188, 1312, 1400, 1750
SseBI	AGGCCT	1 cut(s)	1852
SsiI	CCGC	3 cut(s)	1178, 1487, 1505
SspI	AATATT	1 cut(s)	1027
SspMI	CTAG	7 cut(s)	21, 198, 467, 660, 839, 1496, 1848
StuI	AGGCCT	1 cut(s)	1852
StyD4I	CCNGG	7 cut(s)	689, 744, 1084, 1233, 1319, 1595, 1834
StyI	CCWWGG	3 cut(s)	159, 1471, 1495
TaaI	ACNGT	5 cut(s)	271, 279, 389, 739, 940
TaiI	ACGT	1 cut(s)	592
TaqI	TCGA	2 cut(s)	487, 1414
TasI	AATT	9 cut(s)	70, 357, 653, 1039, 1139, 1188, 1312, 1400, 1750
TatI	WGTACW	2 cut(s)	1457, 1656
TfiI	GAWTC	1 cut(s)	680
TscAI	CASTG	6 cut(s)	274, 808, 1138, 1187, 1431, 1891
TseFI	GTSAC	3 cut(s)	316, 893, 1099
TseI	GCWGC	7 cut(s)	371, 452, 470, 473, 619, 1421, 1430
Tsp45I	GTSAC	3 cut(s)	316, 893, 1099
TspDTI	ATGAA	7 cut(s)	198, 513, 542, 962, 995, 1160, 1701
TspRI	CASTG	6 cut(s)	274, 808, 1138, 1187, 1431, 1891
Tth111I	GACNNNGTC	1 cut(s)	279
Van91I	CCANNNNNTGG	1 cut(s)	353
VpaK11BI	GGWCC	5 cut(s)	344, 628, 990, 1013, 1082
VspI	ATTAAT	1 cut(s)	971
XagI	CCTNNNNNAGG	1 cut(s)	1812
XapI	RAATTY	4 cut(s)	653, 1039, 1139, 1312
XcmI	CCANNNNNNNNNTGG	2 cut(s)	808, 1472
XmaJI	CCTAGG	1 cut(s)	1495
XmiI	GTMKAC	2 cut(s)	260, 1491
XmnI	GAANNNNTTC	1 cut(s)	1376
XspI	CTAG	7 cut(s)	21, 198, 467, 660, 839, 1496, 1848
ZraI	GACGTC	1 cut(s)	590
Zsp2I	ATGCAT	2 cut(s)	1224, 1694

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.