Rroxscaffold_166G00440810

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000166
OG0019102
Physical Location & Seq
Forward (+)
123353 .. 123517
165 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_166G00440810.1

Sequence Viewer

Length: 165 bp
ATGGCGATCGACGAGGATCGGGAGAGCTACGGCGGCGAGCCGCCGCGTTCTCGTCGCGAGCGCGAGCGCGGCCTTGCCACCGAGGCGAAGCTCGAGGTCTACAACGAGGTGCCGAGGAGAATTCGGGAGCTCAATTATGAGGAGACCGGTCTTTCCTGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

54

Amino Acids

6.42

Weight (kDa)

4.93

Isoelectric Point (pI)

60.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 99
AccII CGCG 4 cut(s) 46, 57, 63, 69
AciI CCGC 4 cut(s) 33, 41, 44, 69
AclWI GGATC 1 cut(s) 24
AcsI RAATTY 1 cut(s) 120
AgeI ACCGGT 1 cut(s) 146
AjnI CCWGG 1 cut(s) 155
AluBI AGCT 3 cut(s) 27, 91, 130
AluI AGCT 3 cut(s) 27, 91, 130
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 137
AlwI GGATC 1 cut(s) 24
Ama87I CYCGRG 1 cut(s) 92
AoxI GGCC 1 cut(s) 70
ApoI RAATTY 1 cut(s) 120
AsiGI ACCGGT 1 cut(s) 146
AspLEI GCGC 2 cut(s) 63, 69
AvaI CYCGRG 1 cut(s) 92
BanI GGYRCC 1 cut(s) 109
BanII GRGCYC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 132
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 137
BglI GCCNNNNNGGC 1 cut(s) 83
BisI GCNGC 4 cut(s) 34, 41, 44, 70
BlsI GCNGC 4 cut(s) 35, 42, 45, 71
Bme1390I CCNGG 1 cut(s) 157
BmeT110I CYCGRG 1 cut(s) 92
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 1 cut(s) 157
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 81, 113
BsaWI WCCGGW 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 146
BseBI CCWGG 1 cut(s) 157
BseDI CCNNGG 2 cut(s) 81, 113
BseRI GAGGAG 2 cut(s) 130, 155
Bsh1236I CGCG 4 cut(s) 46, 57, 63, 69
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 72
BshNI GGYRCC 1 cut(s) 109
BshTI ACCGGT 1 cut(s) 146
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 132
BsiHKCI CYCGRG 1 cut(s) 92
BsiSI CCGG 1 cut(s) 147
BsmAI GTCTC 1 cut(s) 137
BsnI GGCC 1 cut(s) 72
Bso31I GGTCTC 1 cut(s) 137
BsoBI CYCGRG 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 2 cut(s) 6, 16
Bsp68I TCGCGA 1 cut(s) 57
BspACI CCGC 4 cut(s) 33, 41, 44, 69
BspANI GGCC 1 cut(s) 72
BspFNI CGCG 4 cut(s) 46, 57, 63, 69
BspLI GGNNCC 1 cut(s) 111
BspPI GGATC 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 137
BsrFI RCCGGY 1 cut(s) 146
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 2 cut(s) 81, 113
BssMI GATC 2 cut(s) 6, 16
Bst2UI CCWGG 1 cut(s) 157
BstC8I GCNNGC 3 cut(s) 38, 59, 65
BstFNI CGCG 4 cut(s) 46, 57, 63, 69
BstHHI GCGC 2 cut(s) 63, 69
BstKTI GATC 2 cut(s) 9, 19
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 2 cut(s) 6, 16
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 3 cut(s) 33, 69, 83
BstNI CCWGG 1 cut(s) 157
BstSCI CCNGG 1 cut(s) 155
BstUI CGCG 4 cut(s) 46, 57, 63, 69
BsuRI GGCC 1 cut(s) 72
BtuMI TCGCGA 1 cut(s) 57
Cac8I GCNNGC 3 cut(s) 38, 59, 65
CfoI GCGC 2 cut(s) 63, 69
Cfr10I RCCGGY 1 cut(s) 146
CspAI ACCGGT 1 cut(s) 146
CviJI RGCY 5 cut(s) 27, 40, 72, 91, 130
CviKI_1 RGCY 5 cut(s) 27, 40, 72, 91, 130
DpnI GATC 2 cut(s) 8, 18
DpnII GATC 2 cut(s) 6, 16
Ecl136II GAGCTC 1 cut(s) 130
Eco24I GRGCYC 1 cut(s) 132
Eco31I GGTCTC 1 cut(s) 137
Eco53kI GAGCTC 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 130
EcoRI GAATTC 1 cut(s) 120
EcoRII CCWGG 1 cut(s) 155
EcoT38I GRGCYC 1 cut(s) 132
FaiI YATR 1 cut(s) 138
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 4 cut(s) 34, 41, 44, 70
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 4 cut(s) 34, 41, 44, 70
GlaI GCGC 2 cut(s) 62, 68
GluI GCNGC 4 cut(s) 34, 41, 44, 70
HaeIII GGCC 1 cut(s) 72
HapII CCGG 1 cut(s) 147
HhaI GCGC 2 cut(s) 63, 69
Hin6I GCGC 2 cut(s) 61, 67
HinP1I GCGC 2 cut(s) 61, 67
HpaII CCGG 1 cut(s) 147
Hpy166II GTNNAC 1 cut(s) 100
Hpy188III TCNNGA 3 cut(s) 20, 56, 125
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 2 cut(s) 14, 57
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 69, 83
HspAI GCGC 2 cut(s) 61, 67
Kzo9I GATC 2 cut(s) 6, 16
LmnI GCTCC 1 cut(s) 127
LpnPI CCDG 2 cut(s) 142, 160
MalI GATC 2 cut(s) 8, 18
MboI GATC 2 cut(s) 6, 16
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 2 cut(s) 120, 133
MnlI CCTC 6 cut(s) 7, 76, 88, 100, 108, 133
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 157
MvaI CCWGG 1 cut(s) 157
MvnI CGCG 4 cut(s) 46, 57, 63, 69
MwoI GCNNNNNNNGC 3 cut(s) 33, 69, 83
NdeII GATC 2 cut(s) 6, 16
NlaIV GGNNCC 1 cut(s) 111
NmeAIII GCCGAG 1 cut(s) 138
NruI TCGCGA 1 cut(s) 57
PaeR7I CTCGAG 1 cut(s) 92
PfoI TCCNGGA 1 cut(s) 155
PinAI ACCGGT 1 cut(s) 146
PkrI GCNGC 4 cut(s) 35, 42, 45, 71
Ple19I CGATCG 1 cut(s) 9
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 155
PspGI CCWGG 1 cut(s) 155
PspN4I GGNNCC 1 cut(s) 111
PspXI VCTCGAGB 1 cut(s) 92
PvuI CGATCG 1 cut(s) 9
RruI TCGCGA 1 cut(s) 57
SacI GAGCTC 1 cut(s) 132
SatI GCNGC 4 cut(s) 34, 41, 44, 70
Sau3AI GATC 2 cut(s) 6, 16
ScrFI CCNGG 1 cut(s) 157
SduI GDGCHC 1 cut(s) 132
SetI ASST 5 cut(s) 29, 93, 99, 111, 132
Sfr274I CTCGAG 1 cut(s) 92
SlaI CTCGAG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 2 cut(s) 120, 133
SsiI CCGC 4 cut(s) 33, 41, 44, 69
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 155
TaqI TCGA 2 cut(s) 9, 93
TasI AATT 2 cut(s) 120, 133
TauI GCSGC 4 cut(s) 36, 43, 46, 72
XapI RAATTY 1 cut(s) 120
XhoI CTCGAG 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.