Rroxscaffold_4G00302840

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0019102
Physical Location & Seq
Reverse (-)
23178567 .. 23179470
904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302840.1

Sequence Viewer

Length: 591 bp
ATGCAAATGAGAGAGTCCTCATCACTAGCTTCAAATCCGAGTCGGATTCACCTGCAGCTGCAATGGCGATCGACGAGGATCGGGAGAGCTACGGCGGCGAGCCGCCGCGTTCTCGTCGCGAGCGCGAGCGCGGCCTTGCCACCGAGCGAAGCTCGAGGTCTACAACGAGGTGCGAGGAGAATTCGGGAGCTCAATTATGAGGAGACCGGTCTTCCTGGATTTGATGATCGGCTTTGGCTCCATTTCAATCGATTACCTTGCGTTGGATGTGAATGTGGAGAGGGCGAGAGGATGTGCTTACACATAAGAGATTGCTGCAACTTGCGAGAGGACCACGCTTACCAAACCGCTTTTGAAGTTCGCACCGTGCAGGTTTATCCTGTGTACAATGGGATTCCTGGTGATTCGGTCCATTCGGATTCTTCAATGAAAGAAGATGCACAAAAAACTGAAGAACTTAAAAGGGTGTTGGAGAAGGAAATTTCAAAATTTAAGGAGTCAAAGCAGTATCCGATTGCTGCAAATAGCGAGGATAACCAAACAAAAATTGAGTCTTCACCCGACGCCCGCAAATACCAACCGATGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

196

Amino Acids

22.27

Weight (kDa)

8.21

Isoelectric Point (pI)

58.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 60
Acc36I ACCTGC 2 cut(s) 60, 361
AccB7I CCANNNNNTGG 1 cut(s) 583
AccI GTMKAC 1 cut(s) 160
AccII CGCG 4 cut(s) 108, 119, 125, 131
AciI CCGC 6 cut(s) 95, 103, 106, 131, 348, 568
AclWI GGATC 1 cut(s) 86
AcsI RAATTY 3 cut(s) 180, 480, 488
AcuI CTGAAG 1 cut(s) 471
AcyI GRCGYC 1 cut(s) 564
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 2 cut(s) 263, 583
AgeI ACCGGT 1 cut(s) 206
AgsI TTSAA 5 cut(s) 33, 247, 356, 426, 486
AjnI CCWGG 2 cut(s) 214, 397
AluBI AGCT 5 cut(s) 29, 58, 89, 152, 190
AluI AGCT 5 cut(s) 29, 58, 89, 152, 190
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 1 cut(s) 86
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 4 cut(s) 55, 58, 315, 518
ApoI RAATTY 3 cut(s) 180, 480, 488
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AsiGI ACCGGT 1 cut(s) 206
AspLEI GCGC 2 cut(s) 125, 131
AspS9I GGNCC 2 cut(s) 331, 409
AsuHPI GGTGA 3 cut(s) 41, 413, 549
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 2 cut(s) 331, 409
BanII GRGCYC 1 cut(s) 192
BbsI GAAGAC 2 cut(s) 203, 546
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 4 cut(s) 45, 67, 302, 505
BccI CCATC 1 cut(s) 577
BceAI ACGGC 1 cut(s) 108
BcgI CGANNNNNNTGC 2 cut(s) 240, 274
BciT130I CCWGG 2 cut(s) 216, 399
BciVI GTATCC 1 cut(s) 519
BcoDI GTCTC 1 cut(s) 197
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 53
BfuAI ACCTGC 2 cut(s) 60, 361
BfuI GTATCC 1 cut(s) 519
BisI GCNGC 8 cut(s) 56, 59, 96, 103, 106, 132, 316, 519
BlsI GCNGC 8 cut(s) 57, 60, 97, 104, 107, 133, 317, 520
Bme1390I CCNGG 2 cut(s) 216, 399
Bme18I GGWCC 2 cut(s) 331, 409
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 2 cut(s) 331, 409
BmiI GGNNCC 1 cut(s) 239
BmrFI CCNGG 2 cut(s) 216, 399
BmsI GCATC 1 cut(s) 427
BpiI GAAGAC 2 cut(s) 203, 546
BplI GAGNNNNNCTC 2 cut(s) 136, 168
Bsa29I ATCGAT 1 cut(s) 250
BsaHI GRCGYC 1 cut(s) 564
BsaI GGTCTC 1 cut(s) 197
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 583
Bse118I RCCGGY 1 cut(s) 206
Bse3DI GCAATG 1 cut(s) 68
BseBI CCWGG 2 cut(s) 216, 399
BseCI ATCGAT 1 cut(s) 250
BseGI GGATG 2 cut(s) 272, 297
BseLI CCNNNNNNNGG 2 cut(s) 263, 583
BseMI GCAATG 1 cut(s) 68
BseRI GAGGAG 2 cut(s) 190, 215
BseXI GCAGC 4 cut(s) 45, 67, 302, 505
BsgI GTGCAG 1 cut(s) 389
Bsh1236I CGCG 4 cut(s) 108, 119, 125, 131
Bsh1285I CGRYCG 1 cut(s) 71
BshFI GGCC 1 cut(s) 134
BshTI ACCGGT 1 cut(s) 206
BshVI ATCGAT 1 cut(s) 250
BsiEI CGRYCG 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 1 cut(s) 207
BslI CCNNNNNNNGG 2 cut(s) 263, 583
BsmAI GTCTC 1 cut(s) 197
BsnI GGCC 1 cut(s) 134
Bso31I GGTCTC 1 cut(s) 197
BsoBI CYCGRG 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 192
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 3 cut(s) 68, 78, 226
Bsp68I TCGCGA 1 cut(s) 119
BspACI CCGC 6 cut(s) 95, 103, 106, 131, 348, 568
BspANI GGCC 1 cut(s) 134
BspDI ATCGAT 1 cut(s) 250
BspFNI CGCG 4 cut(s) 108, 119, 125, 131
BspLI GGNNCC 1 cut(s) 239
BspMAI CTGCAG 1 cut(s) 57
BspMI ACCTGC 2 cut(s) 60, 361
BspPI GGATC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 197
BsrDI GCAATG 1 cut(s) 68
BsrFI RCCGGY 1 cut(s) 206
BsrGI TGTACA 1 cut(s) 384
BssAI RCCGGY 1 cut(s) 206
BssMI GATC 3 cut(s) 68, 78, 226
BssNI GRCGYC 1 cut(s) 564
Bst2UI CCWGG 2 cut(s) 216, 399
Bst4CI ACNGT 1 cut(s) 367
BstACI GRCGYC 1 cut(s) 564
BstAUI TGTACA 1 cut(s) 384
BstC8I GCNNGC 4 cut(s) 100, 121, 127, 568
BstF5I GGATG 2 cut(s) 272, 297
BstFNI CGCG 4 cut(s) 108, 119, 125, 131
BstHHI GCGC 2 cut(s) 125, 131
BstKTI GATC 3 cut(s) 71, 81, 229
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 3 cut(s) 68, 78, 226
BstMCI CGRYCG 1 cut(s) 71
BstMWI GCNNNNNNNGC 3 cut(s) 64, 95, 131
BstNI CCWGG 2 cut(s) 216, 399
BstSCI CCNGG 2 cut(s) 214, 397
BstSFI CTRYAG 1 cut(s) 53
BstUI CGCG 4 cut(s) 108, 119, 125, 131
BstV1I GCAGC 4 cut(s) 45, 67, 302, 505
BstV2I GAAGAC 2 cut(s) 203, 546
Bsu15I ATCGAT 1 cut(s) 250
BsuI GTATCC 1 cut(s) 519
BsuRI GGCC 1 cut(s) 134
BsuTUI ATCGAT 1 cut(s) 250
BtsCI GGATG 2 cut(s) 272, 297
BtuMI TCGCGA 1 cut(s) 119
BveI ACCTGC 2 cut(s) 60, 361
Cac8I GCNNGC 4 cut(s) 100, 121, 127, 568
CfoI GCGC 2 cut(s) 125, 131
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 2 cut(s) 331, 409
ClaI ATCGAT 1 cut(s) 250
CseI GACGC 1 cut(s) 572
Csp6I GTAC 1 cut(s) 385
CspAI ACCGGT 1 cut(s) 206
CviJI RGCY 9 cut(s) 29, 58, 89, 102, 134, 152, 190, 232, 238
CviKI_1 RGCY 9 cut(s) 29, 58, 89, 102, 134, 152, 190, 232, 238
CviQI GTAC 1 cut(s) 385
DpnI GATC 3 cut(s) 70, 80, 228
DpnII GATC 3 cut(s) 68, 78, 226
Ecl136II GAGCTC 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 192
Eco31I GGTCTC 1 cut(s) 197
Eco47I GGWCC 2 cut(s) 331, 409
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 190
EcoRI GAATTC 1 cut(s) 180
EcoRII CCWGG 2 cut(s) 214, 397
EcoT38I GRGCYC 1 cut(s) 192
FaiI YATR 3 cut(s) 198, 305, 589
FauI CCCGC 1 cut(s) 575
FblI GTMKAC 1 cut(s) 160
Fnu4HI GCNGC 8 cut(s) 56, 59, 96, 103, 106, 132, 316, 519
FokI GGATG 2 cut(s) 279, 304
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 8 cut(s) 56, 59, 96, 103, 106, 132, 316, 519
FspBI CTAG 1 cut(s) 26
GlaI GCGC 2 cut(s) 124, 130
GluI GCNGC 8 cut(s) 56, 59, 96, 103, 106, 132, 316, 519
HaeIII GGCC 1 cut(s) 134
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 572
HhaI GCGC 2 cut(s) 125, 131
Hin1I GRCGYC 1 cut(s) 564
Hin6I GCGC 2 cut(s) 123, 129
HinP1I GCGC 2 cut(s) 123, 129
HinfI GANTC 8 cut(s) 14, 40, 46, 394, 404, 419, 497, 551
HpaII CCGG 1 cut(s) 207
HphI GGTGA 3 cut(s) 41, 413, 549
Hpy166II GTNNAC 2 cut(s) 161, 385
Hpy188I TCNGA 4 cut(s) 39, 45, 418, 513
Hpy188III TCNNGA 3 cut(s) 82, 118, 185
Hpy8I GTNNAC 2 cut(s) 161, 385
Hpy99I CGWCG 3 cut(s) 76, 119, 566
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 1 cut(s) 367
HpyCH4V TGCA 7 cut(s) 4, 55, 61, 318, 370, 440, 521
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 95, 131
Hsp92I GRCGYC 1 cut(s) 564
HspAI GCGC 2 cut(s) 123, 129
Kzo9I GATC 3 cut(s) 68, 78, 226
LmnI GCTCC 2 cut(s) 187, 243
LpnPI CCDG 8 cut(s) 65, 201, 220, 228, 356, 384, 393, 411
Lsp1109I GCAGC 4 cut(s) 45, 67, 302, 505
LweI GCATC 1 cut(s) 427
MaeI CTAG 1 cut(s) 26
MalI GATC 3 cut(s) 70, 80, 228
MboI GATC 3 cut(s) 68, 78, 226
MboII GAAGA 5 cut(s) 203, 414, 446, 464, 546
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 5 cut(s) 180, 193, 480, 488, 546
MlyI GAGTC 4 cut(s) 23, 49, 506, 560
MmeI TCCRAC 3 cut(s) 23, 244, 450
MseI TTAA 2 cut(s) 459, 492
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 2 cut(s) 216, 399
MvaI CCWGG 2 cut(s) 216, 399
MvnI CGCG 4 cut(s) 108, 119, 125, 131
MwoI GCNNNNNNNGC 3 cut(s) 64, 95, 131
NdeII GATC 3 cut(s) 68, 78, 226
NlaIV GGNNCC 1 cut(s) 239
NruI TCGCGA 1 cut(s) 119
PaeR7I CTCGAG 1 cut(s) 153
PaqCI CACCTGC 1 cut(s) 60
PfeI GAWTC 4 cut(s) 46, 394, 404, 419
PflMI CCANNNNNTGG 1 cut(s) 583
PfoI TCCNGGA 1 cut(s) 214
PinAI ACCGGT 1 cut(s) 206
PkrI GCNGC 8 cut(s) 57, 60, 97, 104, 107, 133, 317, 520
Ple19I CGATCG 1 cut(s) 71
PleI GAGTC 4 cut(s) 22, 48, 505, 559
PpsI GAGTC 4 cut(s) 22, 48, 505, 559
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 2 cut(s) 214, 397
PspGI CCWGG 2 cut(s) 214, 397
PspN4I GGNNCC 1 cut(s) 239
PspPI GGNCC 2 cut(s) 331, 409
PspXI VCTCGAGB 1 cut(s) 153
PstI CTGCAG 1 cut(s) 57
PvuI CGATCG 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 58
RruI TCGCGA 1 cut(s) 119
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 2 cut(s) 459, 492
SatI GCNGC 8 cut(s) 56, 59, 96, 103, 106, 132, 316, 519
Sau3AI GATC 3 cut(s) 68, 78, 226
Sau96I GGNCC 2 cut(s) 331, 409
SchI GAGTC 4 cut(s) 23, 49, 506, 560
ScrFI CCNGG 2 cut(s) 216, 399
SduI GDGCHC 1 cut(s) 192
SfaNI GCATC 1 cut(s) 427
SfcI CTRYAG 1 cut(s) 53
Sfr274I CTCGAG 1 cut(s) 153
SinI GGWCC 2 cut(s) 331, 409
SlaI CTCGAG 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
Sse9I AATT 5 cut(s) 180, 193, 480, 488, 546
SsiI CCGC 6 cut(s) 95, 103, 106, 131, 348, 568
SspMI CTAG 1 cut(s) 26
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 2 cut(s) 214, 397
TaaI ACNGT 1 cut(s) 367
TaqI TCGA 3 cut(s) 71, 154, 250
TaqII GACCGA 1 cut(s) 397
TasI AATT 5 cut(s) 180, 193, 480, 488, 546
TatI WGTACW 1 cut(s) 384
TauI GCSGC 4 cut(s) 98, 105, 108, 134
TfiI GAWTC 4 cut(s) 46, 394, 404, 419
Tru1I TTAA 2 cut(s) 459, 492
Tru9I TTAA 2 cut(s) 459, 492
TseI GCWGC 4 cut(s) 55, 58, 315, 518
TspDTI ATGAA 1 cut(s) 443
Van91I CCANNNNNTGG 1 cut(s) 583
VpaK11BI GGWCC 2 cut(s) 331, 409
XapI RAATTY 3 cut(s) 180, 480, 488
XhoI CTCGAG 1 cut(s) 153
XmiI GTMKAC 1 cut(s) 160
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.