Rroxscaffold_2G00125450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0019102
Physical Location & Seq
Reverse (-)
60228848 .. 60235096
6249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00125450.1

Sequence Viewer

Length: 378 bp
ATGTACACAACCTCCAAACATATGCATTGTGACCGTGTGGATCACCGAGCTCGGTCACCACTATTTTCAGGCCACTCATTTTGGAGAGTCCTCATCACTAGCTTCAAATCCGAGTCGATTCACTGCGAGCTGCAATGGCGATCGACGAGGCCCGGAGAGCTACGGCGGCGAGCCGCCGCGTTCTCGTCGCGGGCGCGAGCGCGGCCTTGCCACCGAGGCGAAGCTCGAGGTCTACAACGAGGTGCGAGGAGAATTCGGGAGCTCAATTATGAGGAGACCGGTCTTCTGGATTTGATGATCGGCTTTGGCTCCATTTCAATCGATTACCTTGCGTTGGATGTGAATGTGGAGAGGGCGAGAGGATGTGCTTACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

14.44

Weight (kDa)

10.26

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 232
AccII CGCG 4 cut(s) 179, 190, 196, 202
AciI CCGC 5 cut(s) 166, 174, 177, 190, 202
AclWI GGATC 1 cut(s) 48
AcsI RAATTY 1 cut(s) 252
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 334
AgeI ACCGGT 1 cut(s) 278
AgsI TTSAA 2 cut(s) 106, 318
AluBI AGCT 6 cut(s) 50, 102, 130, 160, 224, 262
AluI AGCT 6 cut(s) 50, 102, 130, 160, 224, 262
Alw21I GWGCWC 2 cut(s) 52, 264
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 1 cut(s) 48
Ama87I CYCGRG 1 cut(s) 225
AoxI GGCC 3 cut(s) 70, 149, 203
ApeKI GCWGC 1 cut(s) 130
ApoI RAATTY 1 cut(s) 252
AsiGI ACCGGT 1 cut(s) 278
AspLEI GCGC 2 cut(s) 196, 202
AspS9I GGNCC 1 cut(s) 150
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 2 cut(s) 35, 48
AvaI CYCGRG 1 cut(s) 225
BanII GRGCYC 2 cut(s) 52, 264
BbsI GAAGAC 1 cut(s) 275
Bbv12I GWGCWC 2 cut(s) 52, 264
BbvI GCAGC 1 cut(s) 117
BceAI ACGGC 1 cut(s) 179
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 1 cut(s) 99
BglI GCCNNNNNGGC 1 cut(s) 216
BisI GCNGC 5 cut(s) 131, 167, 174, 177, 203
BlsI GCNGC 5 cut(s) 132, 168, 175, 178, 204
Bme1390I CCNGG 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 225
BmgT120I GGNCC 1 cut(s) 150
BmiI GGNNCC 1 cut(s) 310
BmrFI CCNGG 1 cut(s) 153
BpiI GAAGAC 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 153
Bsa29I ATCGAT 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 1 cut(s) 214
BsaWI WCCGGW 1 cut(s) 278
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse118I RCCGGY 1 cut(s) 278
Bse3DI GCAATG 1 cut(s) 140
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 1 cut(s) 214
BseGI GGATG 2 cut(s) 343, 368
BseLI CCNNNNNNNGG 1 cut(s) 334
BseMI GCAATG 1 cut(s) 140
BseRI GAGGAG 2 cut(s) 262, 287
BseXI GCAGC 1 cut(s) 117
Bsh1236I CGCG 4 cut(s) 179, 190, 196, 202
Bsh1285I CGRYCG 1 cut(s) 143
BshFI GGCC 3 cut(s) 72, 151, 205
BshTI ACCGGT 1 cut(s) 278
BshVI ATCGAT 1 cut(s) 321
BsiEI CGRYCG 1 cut(s) 143
BsiHKAI GWGCWC 2 cut(s) 52, 264
BsiHKCI CYCGRG 1 cut(s) 225
BsiSI CCGG 2 cut(s) 153, 279
BslI CCNNNNNNNGG 1 cut(s) 334
BsmAI GTCTC 1 cut(s) 269
BsnI GGCC 3 cut(s) 72, 151, 205
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 225
Bsp1286I GDGCHC 2 cut(s) 52, 264
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 40, 140, 297
BspACI CCGC 5 cut(s) 166, 174, 177, 190, 202
BspANI GGCC 3 cut(s) 72, 151, 205
BspDI ATCGAT 1 cut(s) 321
BspFNI CGCG 4 cut(s) 179, 190, 196, 202
BspLI GGNNCC 1 cut(s) 310
BspPI GGATC 1 cut(s) 48
BspTNI GGTCTC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 278
BsrGI TGTACA 1 cut(s) 3
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 3 cut(s) 40, 140, 297
Bst4CI ACNGT 1 cut(s) 35
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 4 cut(s) 128, 171, 192, 198
BstEII GGTNACC 1 cut(s) 54
BstF5I GGATG 2 cut(s) 343, 368
BstFNI CGCG 4 cut(s) 179, 190, 196, 202
BstHHI GCGC 2 cut(s) 196, 202
BstKTI GATC 3 cut(s) 43, 143, 300
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 3 cut(s) 40, 140, 297
BstMCI CGRYCG 1 cut(s) 143
BstMWI GCNNNNNNNGC 5 cut(s) 136, 157, 166, 202, 216
BstPI GGTNACC 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 151
BstUI CGCG 4 cut(s) 179, 190, 196, 202
BstV1I GCAGC 1 cut(s) 117
BstV2I GAAGAC 1 cut(s) 275
Bsu15I ATCGAT 1 cut(s) 321
BsuRI GGCC 3 cut(s) 72, 151, 205
BsuTUI ATCGAT 1 cut(s) 321
BtsCI GGATG 2 cut(s) 343, 368
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 1 cut(s) 121
Cac8I GCNNGC 4 cut(s) 128, 171, 192, 198
CfoI GCGC 2 cut(s) 196, 202
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 1 cut(s) 150
ClaI ATCGAT 1 cut(s) 321
Csp6I GTAC 1 cut(s) 4
CspAI ACCGGT 1 cut(s) 278
CspCI CAANNNNNGTGG 2 cut(s) 62, 97
CviQI GTAC 1 cut(s) 4
DpnI GATC 3 cut(s) 42, 142, 299
DpnII GATC 3 cut(s) 40, 140, 297
Ecl136II GAGCTC 2 cut(s) 50, 262
Eco24I GRGCYC 2 cut(s) 52, 264
Eco31I GGTCTC 1 cut(s) 269
Eco53kI GAGCTC 2 cut(s) 50, 262
Eco88I CYCGRG 1 cut(s) 225
Eco91I GGTNACC 1 cut(s) 54
EcoICRI GAGCTC 2 cut(s) 50, 262
EcoO65I GGTNACC 1 cut(s) 54
EcoRI GAATTC 1 cut(s) 252
EcoT22I ATGCAT 1 cut(s) 27
EcoT38I GRGCYC 2 cut(s) 52, 264
FaiI YATR 4 cut(s) 21, 23, 270, 376
FauI CCCGC 1 cut(s) 183
FauNDI CATATG 1 cut(s) 21
FblI GTMKAC 1 cut(s) 232
Fnu4HI GCNGC 5 cut(s) 131, 167, 174, 177, 203
FokI GGATG 1 cut(s) 350
FriOI GRGCYC 2 cut(s) 52, 264
Fsp4HI GCNGC 5 cut(s) 131, 167, 174, 177, 203
FspBI CTAG 1 cut(s) 99
GlaI GCGC 2 cut(s) 195, 201
GluI GCNGC 5 cut(s) 131, 167, 174, 177, 203
HaeIII GGCC 3 cut(s) 72, 151, 205
HapII CCGG 2 cut(s) 153, 279
HhaI GCGC 2 cut(s) 196, 202
Hin6I GCGC 2 cut(s) 194, 200
HinP1I GCGC 2 cut(s) 194, 200
HinfI GANTC 3 cut(s) 87, 113, 118
HpaII CCGG 2 cut(s) 153, 279
HphI GGTGA 2 cut(s) 35, 48
Hpy166II GTNNAC 2 cut(s) 6, 233
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 2 cut(s) 257, 287
Hpy8I GTNNAC 2 cut(s) 6, 233
Hpy99I CGWCG 2 cut(s) 148, 190
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4V TGCA 2 cut(s) 25, 133
HpyF10VI GCNNNNNNNGC 5 cut(s) 136, 157, 166, 202, 216
HspAI GCGC 2 cut(s) 194, 200
Kzo9I GATC 3 cut(s) 40, 140, 297
LmnI GCTCC 2 cut(s) 259, 314
LpnPI CCDG 4 cut(s) 54, 166, 272, 292
Lsp1109I GCAGC 1 cut(s) 117
MaeI CTAG 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 29, 54
MalI GATC 3 cut(s) 42, 142, 299
MboI GATC 3 cut(s) 40, 140, 297
MboII GAAGA 1 cut(s) 275
MhlI GDGCHC 2 cut(s) 52, 264
MluCI AATT 2 cut(s) 252, 265
MlyI GAGTC 2 cut(s) 96, 122
MmeI TCCRAC 1 cut(s) 315
Mph1103I ATGCAT 1 cut(s) 27
MspI CCGG 2 cut(s) 153, 279
MspR9I CCNGG 1 cut(s) 153
MvnI CGCG 4 cut(s) 179, 190, 196, 202
MwoI GCNNNNNNNGC 5 cut(s) 136, 157, 166, 202, 216
NciI CCSGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 21
NdeII GATC 3 cut(s) 40, 140, 297
NlaIV GGNNCC 1 cut(s) 310
NmuCI GTSAC 2 cut(s) 29, 54
NsiI ATGCAT 1 cut(s) 27
PaeR7I CTCGAG 1 cut(s) 225
PfeI GAWTC 1 cut(s) 118
PinAI ACCGGT 1 cut(s) 278
PkrI GCNGC 5 cut(s) 132, 168, 175, 178, 204
Ple19I CGATCG 1 cut(s) 143
PleI GAGTC 2 cut(s) 95, 121
PpsI GAGTC 2 cut(s) 95, 121
Psp124BI GAGCTC 2 cut(s) 52, 264
PspEI GGTNACC 1 cut(s) 54
PspN4I GGNNCC 1 cut(s) 310
PspPI GGNCC 1 cut(s) 150
PspXI VCTCGAGB 1 cut(s) 225
PvuI CGATCG 1 cut(s) 143
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SacI GAGCTC 2 cut(s) 52, 264
SatI GCNGC 5 cut(s) 131, 167, 174, 177, 203
Sau3AI GATC 3 cut(s) 40, 140, 297
Sau96I GGNCC 1 cut(s) 150
SchI GAGTC 2 cut(s) 96, 122
ScrFI CCNGG 1 cut(s) 153
SduI GDGCHC 2 cut(s) 52, 264
Sfr274I CTCGAG 1 cut(s) 225
SlaI CTCGAG 1 cut(s) 225
SmlI CTYRAG 1 cut(s) 225
SmoI CTYRAG 1 cut(s) 225
Sse9I AATT 2 cut(s) 252, 265
SsiI CCGC 5 cut(s) 166, 174, 177, 190, 202
SspMI CTAG 1 cut(s) 99
SstI GAGCTC 2 cut(s) 52, 264
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 35
TaqI TCGA 4 cut(s) 116, 143, 226, 321
TaqII GACCGA 1 cut(s) 42
TasI AATT 2 cut(s) 252, 265
TatI WGTACW 1 cut(s) 3
TauI GCSGC 4 cut(s) 169, 176, 179, 205
TfiI GAWTC 1 cut(s) 118
TscAI CASTG 1 cut(s) 128
TseFI GTSAC 2 cut(s) 29, 54
TseI GCWGC 1 cut(s) 130
Tsp45I GTSAC 2 cut(s) 29, 54
TspRI CASTG 1 cut(s) 128
XapI RAATTY 1 cut(s) 252
XhoI CTCGAG 1 cut(s) 225
XmiI GTMKAC 1 cut(s) 232
XspI CTAG 1 cut(s) 99
Zsp2I ATGCAT 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.