Rroxscaffold_1G00009590

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010510
Physical Location & Seq
Forward (+)
12149525 .. 12153148
3624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00009590.1

Sequence Viewer

Length: 1176 bp
ATGGTCACTGATTCATATTCAGTAAGTAGTGCTCTGAGTCCAGCCAAGCCAACAATGGCTTCGCTTTGTCTCAACTCGTTGCCTCTGCCTCCACCTTCTCAGCCTCGCAAGCTCTTAGAGACTCCTTCTTCTTTTTCTTCTTCATCTCCTTCTTCACTGAACAGTTATGGCAAAACAGAGGCCTCCAAGCCCATAGTCGTCAGTGGAAACCCACCCACTTTTGTTTCTGCTCCCGGTCGCCGAATTGTCGCCGTTGGGGATCTGCATGGAGACCTTGATCAAACAAGATGTGCACTTGAGATGGCTGGTGTGTTGAGTTCTGATGGTGAAGACTTGTGGACAGGCGGGGAAACGGTATTAGTTCAGCTTGGAGATATACTTGATCGAGGTGAGGATGAAATTGCCATTTTGTCATTGCTGCGATCCTTGGATATCCAGGCAAAAGCTGAAGGTGGAGCAGTTTTTCAGGTCAATGGAAATCATGAAAGCATGAATGTGGAGGGGGATTTTCGATTTGTGGACGCTGGAGGATTTGATGAGTGTATTGATTTCTTGGAATACTTGGATGACTGTAGAGATGACTGGGAAGAAGCTTTTGTTGACTGGATTGGTGTCTCTCAAAAGTTGAAAGAAGATAGGAAAATGCCCCAAAGTTATTGGGATCCATGGAATCTAGTGAGGAGGCAAAAGGGTGTGATTGCCAGATCAATCCTTCTAAGACCAGGCGGTCCATTGGCGTGTGAGTTAGCCCGACATGCTGTTGTTCTTAAGGTCAATGATTGGCTCTTCTGTCATGGTGGTCTTGTTCCTCACCACGTTGCATATGGCATAGAGAGAATGAATAAGGAAGTATCTCATTGGATGAGAGGCATCAGTGAGAGTGAGGACGATCCTGACATCCCTTTCATAGCCACAAGGGGATATGATAGTATACACTCTATTCTTGAAAAGACACTACAAGCAGCTGGTGCTAAGGCAATGGTGGTAGGGCATACTCCTCAAACTGTAGGAGTAAACTGTAAATACAATTGCAGCATATGGCGGATTGACGTTGGGATGTCAAGTGGAGTTCTTAATTCAAGACCAGAGGTTCTAGAAATAAGAGGCGATAAAGCAAGAGTTATCAGAAGTGAGAGGGATAGCTTCAGTGAACTCAATGTTGTTGATTATATATAG

Protein Analysis

391

Amino Acids

42.89

Weight (kDa)

4.98

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 81 - 333 4.1e-08 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010510)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07010 AT1G07010 AT1G07010
fragaria_vesca FvH4_3g39710 FvH4_3g39710 FvH4_3g39710 FvH4_3g39710 FvH4_3g39710
malus_domestica MD11G1056900.v1.1
prunus_persica Prupe.6G048800_v2.0.a1
pyrus_communis pycom11g04790
rosa_chinensis RchiOBHm_Chr5g0071581
rosa_laevigata RLG00000036227
rosa_multiflora Rmu_sc0005745.1_g000010
rosa_roxburghii Rroxscaffold_1G00009590
rosa_rugosa Rorug05G0413400
rosa_samantha Rh5AG469300 Rh5BG488500 Rh5CG512500 Rh5DG499300
rosa_wichuraiana Rw5G043610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 726
AccI GTMKAC 1 cut(s) 931
AciI CCGC 3 cut(s) 345, 726, 1042
AclWI GGATC 5 cut(s) 267, 417, 656, 669, 884
AcuI CTGAAG 2 cut(s) 468, 1129
AflII CTTAAG 1 cut(s) 767
AgsI TTSAA 3 cut(s) 628, 947, 1080
AjnI CCWGG 2 cut(s) 435, 721
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
AluBI AGCT 6 cut(s) 112, 367, 446, 593, 965, 1143
AluI AGCT 6 cut(s) 112, 367, 446, 593, 965, 1143
Alw21I GWGCWC 2 cut(s) 34, 295
Alw26I GTCTC 4 cut(s) 74, 113, 264, 619
Alw44I GTGCAC 1 cut(s) 291
AlwI GGATC 5 cut(s) 267, 417, 656, 669, 884
AoxI GGCC 1 cut(s) 180
ApaLI GTGCAC 1 cut(s) 291
ApeKI GCWGC 3 cut(s) 418, 962, 1032
AspS9I GGNCC 1 cut(s) 728
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 3 cut(s) 338, 401, 803
AvaII GGWCC 1 cut(s) 728
BaeGI GKGCMC 1 cut(s) 295
BamHI GGATCC 1 cut(s) 661
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 2 cut(s) 34, 295
BbvI GCAGC 3 cut(s) 405, 974, 1044
BccI CCATC 2 cut(s) 295, 317
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 2 cut(s) 437, 723
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 4 cut(s) 74, 113, 264, 619
BfaI CTAG 2 cut(s) 674, 1094
BfmI CTRYAG 2 cut(s) 571, 1005
BfrI CTTAAG 1 cut(s) 767
BisI GCNGC 3 cut(s) 419, 963, 1033
BlsI GCNGC 3 cut(s) 420, 964, 1034
Bme1390I CCNGG 3 cut(s) 234, 437, 723
Bme18I GGWCC 1 cut(s) 728
BmgT120I GGNCC 1 cut(s) 728
BmiI GGNNCC 1 cut(s) 663
BmrFI CCNGG 3 cut(s) 234, 437, 723
BmrI ACTGGG 1 cut(s) 592
BmsI GCATC 1 cut(s) 879
BmuI ACTGGG 1 cut(s) 592
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 972
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 426, 665
Bse1I ACTGG 2 cut(s) 587, 608
Bse3DI GCAATG 2 cut(s) 413, 984
BseBI CCWGG 2 cut(s) 437, 723
BseDI CCNNGG 2 cut(s) 426, 665
BseGI GGATG 5 cut(s) 400, 571, 867, 897, 1062
BseMI GCAATG 2 cut(s) 413, 984
BseMII CTCAG 2 cut(s) 26, 113
BseNI ACTGG 2 cut(s) 587, 608
BseRI GAGGAG 2 cut(s) 694, 987
BseSI GKGCMC 1 cut(s) 295
BseXI GCAGC 3 cut(s) 405, 974, 1044
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 1 cut(s) 182
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 2 cut(s) 34, 295
BsiSI CCGG 1 cut(s) 234
BsmAI GTCTC 4 cut(s) 74, 113, 264, 619
BsnI GGCC 1 cut(s) 182
Bso31I GGTCTC 1 cut(s) 264
Bsp1286I GDGCHC 2 cut(s) 34, 295
Bsp143I GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
Bsp19I CCATGG 1 cut(s) 665
BspACI CCGC 3 cut(s) 345, 726, 1042
BspANI GGCC 1 cut(s) 182
BspCNI CTCAG 2 cut(s) 27, 112
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 1 cut(s) 663
BspPI GGATC 5 cut(s) 267, 417, 656, 669, 884
BspQI GCTCTTC 1 cut(s) 791
BspTI CTTAAG 1 cut(s) 767
BspTNI GGTCTC 1 cut(s) 264
BsrDI GCAATG 2 cut(s) 413, 984
BsrI ACTGG 2 cut(s) 587, 608
BssECI CCNNGG 2 cut(s) 426, 665
BssMI GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
BssNAI GTATAC 1 cut(s) 932
BssT1I CCWWGG 2 cut(s) 426, 665
Bst1107I GTATAC 1 cut(s) 932
Bst2UI CCWGG 2 cut(s) 437, 723
Bst4CI ACNGT 5 cut(s) 164, 355, 572, 1006, 1019
Bst6I CTCTTC 1 cut(s) 791
BstAFI CTTAAG 1 cut(s) 767
BstAPI GCANNNNNTGC 1 cut(s) 968
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 5 cut(s) 35, 99, 115, 716, 972
BstDSI CCRYGG 1 cut(s) 665
BstF5I GGATG 5 cut(s) 400, 571, 867, 897, 1062
BstKTI GATC 7 cut(s) 262, 280, 385, 425, 664, 707, 892
BstMAI GTCTC 4 cut(s) 74, 113, 264, 619
BstMBI GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 3 cut(s) 109, 755, 968
BstNI CCWGG 2 cut(s) 437, 723
BstNSI RCATGY 1 cut(s) 758
BstSCI CCNGG 3 cut(s) 232, 435, 721
BstSFI CTRYAG 2 cut(s) 571, 1005
BstSLI GKGCMC 1 cut(s) 295
BstV1I GCAGC 3 cut(s) 405, 974, 1044
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 2 cut(s) 259, 661
BstYI RGATCY 2 cut(s) 259, 661
BstZ17I GTATAC 1 cut(s) 932
BsuRI GGCC 1 cut(s) 182
BtgI CCRYGG 1 cut(s) 665
BtsCI GGATG 5 cut(s) 400, 571, 867, 897, 1062
BtsIMutI CAGTG 5 cut(s) 6, 155, 208, 880, 1153
Cac8I GCNNGC 1 cut(s) 110
CciI TCATGA 1 cut(s) 481
Cfr13I GGNCC 1 cut(s) 728
CseI GACGC 1 cut(s) 530
CviAII CATG 6 cut(s) 266, 482, 490, 666, 755, 794
DdeI CTNAG 5 cut(s) 35, 99, 115, 716, 972
DpnI GATC 7 cut(s) 261, 279, 384, 424, 663, 706, 891
DpnII GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
DrdI GACNNNNNNGTC 1 cut(s) 726
DseDI GACNNNNNNGTC 1 cut(s) 726
Eam1104I CTCTTC 1 cut(s) 791
EarI CTCTTC 1 cut(s) 791
EciI GGCGGA 1 cut(s) 1057
Eco130I CCWWGG 2 cut(s) 426, 665
Eco147I AGGCCT 1 cut(s) 182
Eco31I GGTCTC 1 cut(s) 264
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 728
Eco57I CTGAAG 2 cut(s) 468, 1129
EcoRII CCWGG 2 cut(s) 435, 721
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 2 cut(s) 426, 665
ErhI CCWWGG 2 cut(s) 426, 665
FaeI CATG 6 cut(s) 269, 485, 493, 669, 758, 797
FatI CATG 6 cut(s) 265, 481, 489, 665, 754, 793
FauI CCCGC 1 cut(s) 338
FauNDI CATATG 2 cut(s) 823, 1037
FbaI TGATCA 1 cut(s) 277
FblI GTMKAC 1 cut(s) 931
Fnu4HI GCNGC 3 cut(s) 419, 963, 1033
FokI GGATG 5 cut(s) 407, 578, 874, 884, 1069
Fsp4HI GCNGC 3 cut(s) 419, 963, 1033
FspBI CTAG 2 cut(s) 674, 1094
GluI GCNGC 3 cut(s) 419, 963, 1033
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 1 cut(s) 182
HapII CCGG 1 cut(s) 234
HgaI GACGC 1 cut(s) 530
Hin1II CATG 6 cut(s) 269, 485, 493, 669, 758, 797
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 4 cut(s) 11, 37, 121, 670
HpaII CCGG 1 cut(s) 234
HphI GGTGA 3 cut(s) 338, 401, 803
Hpy166II GTNNAC 7 cut(s) 293, 339, 520, 601, 932, 1015, 1151
Hpy188I TCNGA 3 cut(s) 36, 322, 1127
Hpy188III TCNNGA 5 cut(s) 482, 893, 944, 1080, 1094
Hpy8I GTNNAC 7 cut(s) 293, 339, 520, 601, 932, 1015, 1151
HpyAV CCTTC 5 cut(s) 105, 135, 159, 443, 722
HpyCH4III ACNGT 5 cut(s) 164, 355, 572, 1006, 1019
HpyCH4IV ACGT 2 cut(s) 816, 1050
HpyCH4V TGCA 4 cut(s) 265, 293, 821, 1032
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 755, 968
HpyF3I CTNAG 5 cut(s) 35, 99, 115, 716, 972
HpySE526I ACGT 2 cut(s) 816, 1050
Hsp92II CATG 6 cut(s) 269, 485, 493, 669, 758, 797
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
LguI GCTCTTC 1 cut(s) 791
LmnI GCTCC 2 cut(s) 235, 455
Lsp1109I GCAGC 3 cut(s) 405, 974, 1044
LweI GCATC 1 cut(s) 879
MaeI CTAG 2 cut(s) 674, 1094
MaeII ACGT 2 cut(s) 816, 1050
MaeIII GTNAC 1 cut(s) 4
MalI GATC 7 cut(s) 261, 279, 384, 424, 663, 706, 891
MboI GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
MboII GAAGA 8 cut(s) 120, 129, 132, 144, 341, 599, 644, 778
MfeI CAATTG 1 cut(s) 1027
MflI RGATCY 2 cut(s) 259, 661
MhlI GDGCHC 2 cut(s) 34, 295
MluCI AATT 4 cut(s) 243, 399, 1027, 1075
MlyI GAGTC 2 cut(s) 46, 115
MseI TTAA 2 cut(s) 768, 1074
MslI CAYNNNNRTG 2 cut(s) 494, 736
MspA1I CMGCKG 1 cut(s) 965
MspCI CTTAAG 1 cut(s) 767
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 3 cut(s) 234, 437, 723
MunI CAATTG 1 cut(s) 1027
MvaI CCWGG 2 cut(s) 437, 723
MwoI GCNNNNNNNGC 3 cut(s) 109, 755, 968
NciI CCSGG 1 cut(s) 234
NcoI CCATGG 1 cut(s) 665
NdeI CATATG 2 cut(s) 823, 1037
NdeII GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
NlaIII CATG 6 cut(s) 269, 485, 493, 669, 758, 797
NlaIV GGNNCC 1 cut(s) 663
NmuCI GTSAC 1 cut(s) 4
NspI RCATGY 1 cut(s) 758
PagI TCATGA 1 cut(s) 481
PceI AGGCCT 1 cut(s) 182
PciSI GCTCTTC 1 cut(s) 791
PfeI GAWTC 2 cut(s) 11, 670
PkrI GCNGC 3 cut(s) 420, 964, 1034
PleI GAGTC 2 cut(s) 45, 115
PpsI GAGTC 2 cut(s) 45, 115
Psp6I CCWGG 2 cut(s) 435, 721
PspGI CCWGG 2 cut(s) 435, 721
PspN4I GGNNCC 1 cut(s) 663
PspPI GGNCC 1 cut(s) 728
PsuI RGATCY 2 cut(s) 259, 661
PvuII CAGCTG 1 cut(s) 965
RseI CAYNNNNRTG 2 cut(s) 494, 736
SapI GCTCTTC 1 cut(s) 791
SaqAI TTAA 2 cut(s) 768, 1074
SatI GCNGC 3 cut(s) 419, 963, 1033
Sau3AI GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
Sau96I GGNCC 1 cut(s) 728
SchI GAGTC 2 cut(s) 46, 115
ScrFI CCNGG 3 cut(s) 234, 437, 723
SduI GDGCHC 2 cut(s) 34, 295
SfaNI GCATC 1 cut(s) 879
SfcI CTRYAG 2 cut(s) 571, 1005
SinI GGWCC 1 cut(s) 728
SmiMI CAYNNNNRTG 2 cut(s) 494, 736
SmlI CTYRAG 2 cut(s) 296, 767
SmoI CTYRAG 2 cut(s) 296, 767
Sse9I AATT 4 cut(s) 243, 399, 1027, 1075
SseBI AGGCCT 1 cut(s) 182
SsiI CCGC 3 cut(s) 345, 726, 1042
SspMI CTAG 2 cut(s) 674, 1094
StuI AGGCCT 1 cut(s) 182
StyD4I CCNGG 3 cut(s) 232, 435, 721
StyI CCWWGG 2 cut(s) 426, 665
TaaI ACNGT 5 cut(s) 164, 355, 572, 1006, 1019
TaiI ACGT 2 cut(s) 819, 1053
TaqI TCGA 2 cut(s) 385, 511
TasI AATT 4 cut(s) 243, 399, 1027, 1075
TfiI GAWTC 2 cut(s) 11, 670
Tru1I TTAA 2 cut(s) 768, 1074
Tru9I TTAA 2 cut(s) 768, 1074
TscAI CASTG 5 cut(s) 13, 162, 208, 880, 1153
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 3 cut(s) 418, 962, 1032
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 6 cut(s) 132, 411, 498, 506, 854, 895
TspRI CASTG 5 cut(s) 13, 162, 208, 880, 1153
Vha464I CTTAAG 1 cut(s) 767
VneI GTGCAC 1 cut(s) 291
VpaK11BI GGWCC 1 cut(s) 728
XbaI TCTAGA 1 cut(s) 1093
XceI RCATGY 1 cut(s) 758
XcmI CCANNNNNNNNNTGG 2 cut(s) 52, 821
XmiI GTMKAC 1 cut(s) 931
XspI CTAG 2 cut(s) 674, 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.