Rh5AG469300

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010510
Physical Location & Seq
Reverse (-)
80456507 .. 80460120
3614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG469300.1

Sequence Viewer

Length: 828 bp
ATGGTCACTGATTCATATTCGGTAAGTAGTGCTCTGAGTCCAGCCAAGCCAACAATGGCTTCGCTTTGTCTCAACTCGTTGCCTCTGCCTCCACCTTCTCAGCCTCGCAAGCTCTTAGAGACTCCTCCTTCTTTTTCTTCTTCATCTCCTTCTTCACTGAACAGTTATGGCAAAACAGAGGCCTCCAAGCCCATAGTCGTCAGTGGAAACCCACCCACTTTTGTTTCTGCTCCCGGTCGCCGAATTGTCGCCGTTGGGGATCTGCATGGAGACCTTGATCAAACAAGATGTGCACTTGAGATGGCTGGTGTGTTGAGTTCTGATGGTGAAGACTTGTGGACAGGCGGGGAAACGGTATTAGTTCAGCTTGGAGATATACTTGATCGAGGTGAGGATGAAATTGCCATTTTGTCATTGCTGCGATCCTTGGATATCCAGGCAAAAGCTGAAGGTGGAGCAGTTTTTCAGGTCAATGGAAATCACGAAAGCATGAATGTGGAGGGGGATTTTCGATTTGTGGACGCTGGAGGATTTGATGAGTGTATTGATTTCTTGGAATACTTGGATGACTGTAGAGATGACTGGGAAGAAGCTTTTGTTGACTGGATTGGTGTCTCTCAAAAGTTGAAAGAAGATAGGAAAATGCCCCAAAGTTATTGGGATCCATGGAATCTAGTGAGGAGGCAAAAGGGTGTGATTGCCAGATCAATCCTTCTAAGACCAGGCGGTCCATTGGCGTGTGAGTTGGCGCGACATGCTGTTGTTCTTAAGGTCAATGATTGGCTCTTCTGTCATGGTGGTCTTGTTCCTCACCACGGTAAAAAATAA

Protein Analysis

275

Amino Acids

29.94

Weight (kDa)

4.94

Isoelectric Point (pI)

50.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 81 - 228 5.1e-08 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010510)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07010 AT1G07010 AT1G07010
fragaria_vesca FvH4_3g39710 FvH4_3g39710 FvH4_3g39710 FvH4_3g39710 FvH4_3g39710
malus_domestica MD11G1056900.v1.1
prunus_persica Prupe.6G048800_v2.0.a1
pyrus_communis pycom11g04790
rosa_chinensis RchiOBHm_Chr5g0071581
rosa_laevigata RLG00000036227
rosa_multiflora Rmu_sc0005745.1_g000010
rosa_roxburghii Rroxscaffold_1G00009590
rosa_rugosa Rorug05G0413400
rosa_samantha Rh5AG469300 Rh5BG488500 Rh5CG512500 Rh5DG499300
rosa_wichuraiana Rw5G043610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 726
AccII CGCG 1 cut(s) 751
AciI CCGC 2 cut(s) 345, 726
AclWI GGATC 4 cut(s) 267, 417, 656, 669
AcuI CTGAAG 1 cut(s) 468
AfiI CCNNNNNNNGG 1 cut(s) 815
AflII CTTAAG 1 cut(s) 767
AgsI TTSAA 1 cut(s) 628
AjnI CCWGG 2 cut(s) 435, 721
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
AluBI AGCT 4 cut(s) 112, 367, 446, 593
AluI AGCT 4 cut(s) 112, 367, 446, 593
Alw21I GWGCWC 2 cut(s) 34, 295
Alw26I GTCTC 4 cut(s) 74, 113, 264, 619
Alw44I GTGCAC 1 cut(s) 291
AlwI GGATC 4 cut(s) 267, 417, 656, 669
AoxI GGCC 1 cut(s) 180
ApaLI GTGCAC 1 cut(s) 291
ApeKI GCWGC 1 cut(s) 418
AspLEI GCGC 1 cut(s) 751
AspS9I GGNCC 1 cut(s) 728
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 3 cut(s) 338, 401, 803
AvaII GGWCC 1 cut(s) 728
BaeGI GKGCMC 1 cut(s) 295
BamHI GGATCC 1 cut(s) 661
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 2 cut(s) 34, 295
BbvI GCAGC 1 cut(s) 405
BccI CCATC 2 cut(s) 295, 317
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 2 cut(s) 437, 723
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 4 cut(s) 74, 113, 264, 619
BfaI CTAG 1 cut(s) 674
BfmI CTRYAG 1 cut(s) 571
BfrI CTTAAG 1 cut(s) 767
BisI GCNGC 1 cut(s) 419
BlsI GCNGC 1 cut(s) 420
Bme1390I CCNGG 3 cut(s) 234, 437, 723
Bme18I GGWCC 1 cut(s) 728
BmgT120I GGNCC 1 cut(s) 728
BmiI GGNNCC 1 cut(s) 663
BmrFI CCNGG 3 cut(s) 234, 437, 723
BmrI ACTGGG 1 cut(s) 592
BmuI ACTGGG 1 cut(s) 592
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 264
BsaJI CCNNGG 3 cut(s) 426, 665, 814
Bsc4I CCNNNNNNNGG 1 cut(s) 815
Bse1I ACTGG 2 cut(s) 587, 608
Bse3DI GCAATG 1 cut(s) 413
BseBI CCWGG 2 cut(s) 437, 723
BseDI CCNNGG 3 cut(s) 426, 665, 814
BseGI GGATG 2 cut(s) 400, 571
BseLI CCNNNNNNNGG 1 cut(s) 815
BseMI GCAATG 1 cut(s) 413
BseMII CTCAG 2 cut(s) 26, 113
BseNI ACTGG 2 cut(s) 587, 608
BseRI GAGGAG 2 cut(s) 114, 694
BseSI GKGCMC 1 cut(s) 295
BseXI GCAGC 1 cut(s) 405
Bsh1236I CGCG 1 cut(s) 751
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 1 cut(s) 182
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 2 cut(s) 34, 295
BsiSI CCGG 1 cut(s) 234
BslI CCNNNNNNNGG 1 cut(s) 815
BsmAI GTCTC 4 cut(s) 74, 113, 264, 619
BsnI GGCC 1 cut(s) 182
Bso31I GGTCTC 1 cut(s) 264
Bsp1286I GDGCHC 2 cut(s) 34, 295
Bsp143I GATC 6 cut(s) 259, 277, 382, 422, 661, 704
Bsp19I CCATGG 1 cut(s) 665
BspACI CCGC 2 cut(s) 345, 726
BspANI GGCC 1 cut(s) 182
BspCNI CTCAG 2 cut(s) 27, 112
BspFNI CGCG 1 cut(s) 751
BspLI GGNNCC 1 cut(s) 663
BspPI GGATC 4 cut(s) 267, 417, 656, 669
BspQI GCTCTTC 1 cut(s) 791
BspTI CTTAAG 1 cut(s) 767
BspTNI GGTCTC 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 413
BsrI ACTGG 2 cut(s) 587, 608
BssECI CCNNGG 3 cut(s) 426, 665, 814
BssMI GATC 6 cut(s) 259, 277, 382, 422, 661, 704
BssT1I CCWWGG 2 cut(s) 426, 665
Bst2UI CCWGG 2 cut(s) 437, 723
Bst4CI ACNGT 4 cut(s) 164, 355, 572, 818
Bst6I CTCTTC 1 cut(s) 791
BstAFI CTTAAG 1 cut(s) 767
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 4 cut(s) 35, 99, 115, 716
BstDSI CCRYGG 2 cut(s) 665, 814
BstF5I GGATG 2 cut(s) 400, 571
BstFNI CGCG 1 cut(s) 751
BstHHI GCGC 1 cut(s) 751
BstKTI GATC 6 cut(s) 262, 280, 385, 425, 664, 707
BstMAI GTCTC 4 cut(s) 74, 113, 264, 619
BstMBI GATC 6 cut(s) 259, 277, 382, 422, 661, 704
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 2 cut(s) 109, 755
BstNI CCWGG 2 cut(s) 437, 723
BstNSI RCATGY 1 cut(s) 758
BstSCI CCNGG 3 cut(s) 232, 435, 721
BstSFI CTRYAG 1 cut(s) 571
BstSLI GKGCMC 1 cut(s) 295
BstUI CGCG 1 cut(s) 751
BstV1I GCAGC 1 cut(s) 405
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 2 cut(s) 259, 661
BstYI RGATCY 2 cut(s) 259, 661
BsuRI GGCC 1 cut(s) 182
BtgI CCRYGG 2 cut(s) 665, 814
BtsCI GGATG 2 cut(s) 400, 571
BtsIMutI CAGTG 3 cut(s) 6, 155, 208
Cac8I GCNNGC 1 cut(s) 110
CfoI GCGC 1 cut(s) 751
Cfr13I GGNCC 1 cut(s) 728
CseI GACGC 1 cut(s) 530
CviAII CATG 5 cut(s) 266, 490, 666, 755, 794
DdeI CTNAG 4 cut(s) 35, 99, 115, 716
DpnI GATC 6 cut(s) 261, 279, 384, 424, 663, 706
DpnII GATC 6 cut(s) 259, 277, 382, 422, 661, 704
DrdI GACNNNNNNGTC 1 cut(s) 726
DseDI GACNNNNNNGTC 1 cut(s) 726
Eam1104I CTCTTC 1 cut(s) 791
EarI CTCTTC 1 cut(s) 791
Eco130I CCWWGG 2 cut(s) 426, 665
Eco147I AGGCCT 1 cut(s) 182
Eco31I GGTCTC 1 cut(s) 264
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 728
Eco57I CTGAAG 1 cut(s) 468
EcoRII CCWGG 2 cut(s) 435, 721
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 2 cut(s) 426, 665
ErhI CCWWGG 2 cut(s) 426, 665
FaeI CATG 5 cut(s) 269, 493, 669, 758, 797
FaiI YATR 9 cut(s) 16, 168, 194, 267, 377, 491, 667, 756, 795
FatI CATG 5 cut(s) 265, 489, 665, 754, 793
FauI CCCGC 1 cut(s) 338
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 1 cut(s) 419
FokI GGATG 2 cut(s) 407, 578
Fsp4HI GCNGC 1 cut(s) 419
FspBI CTAG 1 cut(s) 674
GlaI GCGC 1 cut(s) 750
GluI GCNGC 1 cut(s) 419
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 1 cut(s) 182
HapII CCGG 1 cut(s) 234
HgaI GACGC 1 cut(s) 530
HhaI GCGC 1 cut(s) 751
Hin1II CATG 5 cut(s) 269, 493, 669, 758, 797
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 4 cut(s) 11, 37, 121, 670
HpaII CCGG 1 cut(s) 234
HphI GGTGA 3 cut(s) 338, 401, 803
Hpy166II GTNNAC 4 cut(s) 293, 339, 520, 601
Hpy188I TCNGA 2 cut(s) 36, 322
Hpy188III TCNNGA 1 cut(s) 482
Hpy8I GTNNAC 4 cut(s) 293, 339, 520, 601
HpyAV CCTTC 5 cut(s) 105, 138, 159, 443, 722
HpyCH4III ACNGT 4 cut(s) 164, 355, 572, 818
HpyCH4V TGCA 2 cut(s) 265, 293
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 755
HpyF3I CTNAG 4 cut(s) 35, 99, 115, 716
Hsp92II CATG 5 cut(s) 269, 493, 669, 758, 797
HspAI GCGC 1 cut(s) 749
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 6 cut(s) 259, 277, 382, 422, 661, 704
LguI GCTCTTC 1 cut(s) 791
LmnI GCTCC 2 cut(s) 235, 455
Lsp1109I GCAGC 1 cut(s) 405
MaeI CTAG 1 cut(s) 674
MaeIII GTNAC 1 cut(s) 4
MalI GATC 6 cut(s) 261, 279, 384, 424, 663, 706
MboI GATC 6 cut(s) 259, 277, 382, 422, 661, 704
MboII GAAGA 7 cut(s) 129, 132, 144, 341, 599, 644, 778
MflI RGATCY 2 cut(s) 259, 661
MhlI GDGCHC 2 cut(s) 34, 295
MluCI AATT 2 cut(s) 243, 399
MlyI GAGTC 2 cut(s) 46, 115
MseI TTAA 1 cut(s) 768
MslI CAYNNNNRTG 2 cut(s) 494, 736
MspCI CTTAAG 1 cut(s) 767
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 3 cut(s) 234, 437, 723
MvaI CCWGG 2 cut(s) 437, 723
MvnI CGCG 1 cut(s) 751
MwoI GCNNNNNNNGC 2 cut(s) 109, 755
NciI CCSGG 1 cut(s) 234
NcoI CCATGG 1 cut(s) 665
NdeII GATC 6 cut(s) 259, 277, 382, 422, 661, 704
NlaIII CATG 5 cut(s) 269, 493, 669, 758, 797
NlaIV GGNNCC 1 cut(s) 663
NmuCI GTSAC 1 cut(s) 4
NspI RCATGY 1 cut(s) 758
PceI AGGCCT 1 cut(s) 182
PciSI GCTCTTC 1 cut(s) 791
PfeI GAWTC 2 cut(s) 11, 670
PkrI GCNGC 1 cut(s) 420
PleI GAGTC 2 cut(s) 45, 115
PpsI GAGTC 2 cut(s) 45, 115
Psp6I CCWGG 2 cut(s) 435, 721
PspGI CCWGG 2 cut(s) 435, 721
PspN4I GGNNCC 1 cut(s) 663
PspPI GGNCC 1 cut(s) 728
PsuI RGATCY 2 cut(s) 259, 661
RseI CAYNNNNRTG 2 cut(s) 494, 736
SapI GCTCTTC 1 cut(s) 791
SaqAI TTAA 1 cut(s) 768
SatI GCNGC 1 cut(s) 419
Sau3AI GATC 6 cut(s) 259, 277, 382, 422, 661, 704
Sau96I GGNCC 1 cut(s) 728
SchI GAGTC 2 cut(s) 46, 115
ScrFI CCNGG 3 cut(s) 234, 437, 723
SduI GDGCHC 2 cut(s) 34, 295
SfcI CTRYAG 1 cut(s) 571
SinI GGWCC 1 cut(s) 728
SmiMI CAYNNNNRTG 2 cut(s) 494, 736
SmlI CTYRAG 2 cut(s) 296, 767
SmoI CTYRAG 2 cut(s) 296, 767
Sse9I AATT 2 cut(s) 243, 399
SseBI AGGCCT 1 cut(s) 182
SsiI CCGC 2 cut(s) 345, 726
SspMI CTAG 1 cut(s) 674
StuI AGGCCT 1 cut(s) 182
StyD4I CCNGG 3 cut(s) 232, 435, 721
StyI CCWWGG 2 cut(s) 426, 665
TaaI ACNGT 4 cut(s) 164, 355, 572, 818
TaqI TCGA 2 cut(s) 385, 511
TasI AATT 2 cut(s) 243, 399
TfiI GAWTC 2 cut(s) 11, 670
Tru1I TTAA 1 cut(s) 768
Tru9I TTAA 1 cut(s) 768
TscAI CASTG 3 cut(s) 13, 162, 208
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 1 cut(s) 418
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 3 cut(s) 132, 411, 506
TspRI CASTG 3 cut(s) 13, 162, 208
Vha464I CTTAAG 1 cut(s) 767
VneI GTGCAC 1 cut(s) 291
VpaK11BI GGWCC 1 cut(s) 728
XceI RCATGY 1 cut(s) 758
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XspI CTAG 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.