Rw5G043610

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010510
Physical Location & Seq
Reverse (-)
76485692 .. 76488773
3082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G043610.1

Sequence Viewer

Length: 1239 bp
ATGGTCACTGATTCATATTCAGTAAGTAGTGCTCTGAGTCCAGCCAAGCCAACAATGGCTTCGCTTTGTCTCAACTCGTTGCCTCTGCCTCCACCTTCTCAGCCTCGCAAGCTCTTAGAGACTCCTCCTTCTTTTTCTTCTTCATCTCCTTCTTCACTGAACAGTTACGGCAAAACAGAGGCCTCCAAGCCCATAGTCGTCAGTGGAAACCCACCCACTTTTGTTTCTGCTCCCGGTCGCCGAATTGTCGCCGTTGGGGATCTGCATGGAGACCTTGATCAAACAAGATGTGCACTTGAGATGGCTGGTGTGTTGAGTTCTGATGGTGAAGACTTGTGGACAGGCGGGGAAACGGTATTAGTTCAGCTTGGAGATATACTTGATCGAGGTGAGGATGAAATTGCCATTTTGTCATTGCTGCGATCCTTGGATATCCAGGCAAAAGCTGAAGGTGGAGCAGTTTTTCAGGTCAATGGAAATCACGAAAGCATGAATGTGGAGGGGGATTTTCGATTTGTGGACGCTGGAGGATTTGATGAGTGTATTGATTTCTTGGAATACTTGGATGACTGTAGAGATGACTGGGAAGAAGCTTTTGTTGACTGGATTGGTGTCTCTCAAAAGTTGAAAGAAGATAGGAAAAAGCCCCAAAGTTATTGGGATCCATGGAATCTAGTGAGGAGGCAAAAGGGTGTGATTGCCAGATCAATCCTTCTAAGACCAGGCGGTCCATTGGCGTGTGAGTTAGCGCGACATGCTGTTGTTCTTAAGGTCAATGATTGGCTCTTCTGTCATGGTGGTCTTGTTCCTCACCACGTTGCATATGGCATAGAGAGAATGAATAAGGAAGTATCTCATTGGATGAGAGGCCTCAGTGAGAGTGAGGACGATCCTGACATCCCTTTCATAGCCACAAGGGGATATGACAGTGTAGTTTGGAATCGTTTGTACTCGAGAGATAACTCAGATTTCGATGACTACCAGAGTAACCAGATACACTCTATTCTTGAAAAGACACTACAAGCAGCTGGTGCTAAGGCAATGGTTGTAGGGCATACTCCTCAAACTGTAGGAGTAAACTGTAAATACAATTGTAGCATATGGCGGATTGACGTTGGGATGTCAAGTGGAGTTCTTAATTCAAGACCGGAGGTTCTAGAAATAAGAGGCGATAAAGCAAGAGTTATCAGAAGTGAGAGGGATAGCTTCAGTGAACTCAGTGTTGTTGATTATATATAG

Protein Analysis

412

Amino Acids

45.47

Weight (kDa)

4.95

Isoelectric Point (pI)

47.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 81 - 353 2.1e-07 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010510)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07010 AT1G07010 AT1G07010
fragaria_vesca FvH4_3g39710 FvH4_3g39710 FvH4_3g39710 FvH4_3g39710 FvH4_3g39710
malus_domestica MD11G1056900.v1.1
prunus_persica Prupe.6G048800_v2.0.a1
pyrus_communis pycom11g04790
rosa_chinensis RchiOBHm_Chr5g0071581
rosa_laevigata RLG00000036227
rosa_multiflora Rmu_sc0005745.1_g000010
rosa_roxburghii Rroxscaffold_1G00009590
rosa_rugosa Rorug05G0413400
rosa_samantha Rh5AG469300 Rh5BG488500 Rh5CG512500 Rh5DG499300
rosa_wichuraiana Rw5G043610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 726
AccII CGCG 1 cut(s) 751
AciI CCGC 3 cut(s) 345, 726, 1105
AclWI GGATC 5 cut(s) 267, 417, 656, 669, 884
AcuI CTGAAG 2 cut(s) 468, 1192
AfaI GTAC 1 cut(s) 950
AflII CTTAAG 1 cut(s) 767
AgsI TTSAA 3 cut(s) 628, 1010, 1143
AjnI CCWGG 2 cut(s) 435, 721
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
AluBI AGCT 6 cut(s) 112, 367, 446, 593, 1028, 1206
AluI AGCT 6 cut(s) 112, 367, 446, 593, 1028, 1206
Alw21I GWGCWC 2 cut(s) 34, 295
Alw26I GTCTC 4 cut(s) 74, 113, 264, 619
Alw44I GTGCAC 1 cut(s) 291
AlwI GGATC 5 cut(s) 267, 417, 656, 669, 884
Ama87I CYCGRG 1 cut(s) 952
AoxI GGCC 2 cut(s) 180, 868
ApaLI GTGCAC 1 cut(s) 291
ApeKI GCWGC 2 cut(s) 418, 1025
AspLEI GCGC 1 cut(s) 751
AspS9I GGNCC 1 cut(s) 728
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 3 cut(s) 338, 401, 803
AvaI CYCGRG 1 cut(s) 952
AvaII GGWCC 1 cut(s) 728
BaeGI GKGCMC 1 cut(s) 295
BamHI GGATCC 1 cut(s) 661
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 2 cut(s) 34, 295
BbvI GCAGC 2 cut(s) 405, 1037
BccI CCATC 2 cut(s) 295, 317
BceAI ACGGC 2 cut(s) 184, 236
BciT130I CCWGG 2 cut(s) 437, 723
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 4 cut(s) 74, 113, 264, 619
BfaI CTAG 2 cut(s) 674, 1157
BfmI CTRYAG 2 cut(s) 571, 1068
BfrI CTTAAG 1 cut(s) 767
BisI GCNGC 2 cut(s) 419, 1026
BlsI GCNGC 2 cut(s) 420, 1027
Bme1390I CCNGG 3 cut(s) 234, 437, 723
Bme18I GGWCC 1 cut(s) 728
BmeT110I CYCGRG 1 cut(s) 952
BmgT120I GGNCC 1 cut(s) 728
BmiI GGNNCC 1 cut(s) 663
BmrFI CCNGG 3 cut(s) 234, 437, 723
BmrI ACTGGG 1 cut(s) 592
BmuI ACTGGG 1 cut(s) 592
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 1035
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 426, 665
BsaWI WCCGGW 1 cut(s) 1147
Bse1I ACTGG 2 cut(s) 587, 608
Bse3DI GCAATG 2 cut(s) 413, 1047
BseBI CCWGG 2 cut(s) 437, 723
BseDI CCNNGG 2 cut(s) 426, 665
BseGI GGATG 5 cut(s) 400, 571, 867, 897, 1125
BseMI GCAATG 2 cut(s) 413, 1047
BseMII CTCAG 5 cut(s) 26, 113, 886, 978, 1231
BseNI ACTGG 2 cut(s) 587, 608
BseRI GAGGAG 3 cut(s) 114, 694, 1050
BseSI GKGCMC 1 cut(s) 295
BseXI GCAGC 2 cut(s) 405, 1037
Bsh1236I CGCG 1 cut(s) 751
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 2 cut(s) 182, 870
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 2 cut(s) 34, 295
BsiHKCI CYCGRG 1 cut(s) 952
BsiSI CCGG 2 cut(s) 234, 1148
BsmAI GTCTC 4 cut(s) 74, 113, 264, 619
BsnI GGCC 2 cut(s) 182, 870
Bso31I GGTCTC 1 cut(s) 264
BsoBI CYCGRG 1 cut(s) 952
Bsp1286I GDGCHC 2 cut(s) 34, 295
Bsp143I GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
Bsp19I CCATGG 1 cut(s) 665
BspACI CCGC 3 cut(s) 345, 726, 1105
BspANI GGCC 2 cut(s) 182, 870
BspCNI CTCAG 5 cut(s) 27, 112, 885, 977, 1230
BspFNI CGCG 1 cut(s) 751
BspLI GGNNCC 1 cut(s) 663
BspPI GGATC 5 cut(s) 267, 417, 656, 669, 884
BspQI GCTCTTC 1 cut(s) 791
BspTI CTTAAG 1 cut(s) 767
BspTNI GGTCTC 1 cut(s) 264
BsrDI GCAATG 2 cut(s) 413, 1047
BsrI ACTGG 2 cut(s) 587, 608
BssECI CCNNGG 2 cut(s) 426, 665
BssMI GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
BssT1I CCWWGG 2 cut(s) 426, 665
Bst2UI CCWGG 2 cut(s) 437, 723
Bst4CI ACNGT 6 cut(s) 164, 355, 572, 929, 1069, 1082
Bst6I CTCTTC 1 cut(s) 791
BstAFI CTTAAG 1 cut(s) 767
BstAPI GCANNNNNTGC 1 cut(s) 1031
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 8 cut(s) 35, 99, 115, 716, 872, 964, 1035, 1217
BstDSI CCRYGG 1 cut(s) 665
BstF5I GGATG 5 cut(s) 400, 571, 867, 897, 1125
BstFNI CGCG 1 cut(s) 751
BstHHI GCGC 1 cut(s) 751
BstKTI GATC 7 cut(s) 262, 280, 385, 425, 664, 707, 892
BstMAI GTCTC 4 cut(s) 74, 113, 264, 619
BstMBI GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 3 cut(s) 109, 755, 1031
BstNI CCWGG 2 cut(s) 437, 723
BstNSI RCATGY 1 cut(s) 758
BstSCI CCNGG 3 cut(s) 232, 435, 721
BstSFI CTRYAG 2 cut(s) 571, 1068
BstSLI GKGCMC 1 cut(s) 295
BstUI CGCG 1 cut(s) 751
BstV1I GCAGC 2 cut(s) 405, 1037
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 2 cut(s) 259, 661
BstYI RGATCY 2 cut(s) 259, 661
BsuRI GGCC 2 cut(s) 182, 870
BtgI CCRYGG 1 cut(s) 665
BtsCI GGATG 5 cut(s) 400, 571, 867, 897, 1125
BtsIMutI CAGTG 7 cut(s) 6, 155, 208, 880, 934, 1216, 1225
Cac8I GCNNGC 1 cut(s) 110
CfoI GCGC 1 cut(s) 751
Cfr13I GGNCC 1 cut(s) 728
CseI GACGC 1 cut(s) 530
Csp6I GTAC 1 cut(s) 949
CviAII CATG 5 cut(s) 266, 490, 666, 755, 794
CviQI GTAC 1 cut(s) 949
DdeI CTNAG 8 cut(s) 35, 99, 115, 716, 872, 964, 1035, 1217
DpnI GATC 7 cut(s) 261, 279, 384, 424, 663, 706, 891
DpnII GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
DrdI GACNNNNNNGTC 1 cut(s) 726
DseDI GACNNNNNNGTC 1 cut(s) 726
Eam1104I CTCTTC 1 cut(s) 791
EarI CTCTTC 1 cut(s) 791
EciI GGCGGA 1 cut(s) 1120
Eco130I CCWWGG 2 cut(s) 426, 665
Eco147I AGGCCT 2 cut(s) 182, 870
Eco31I GGTCTC 1 cut(s) 264
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 728
Eco57I CTGAAG 2 cut(s) 468, 1192
Eco88I CYCGRG 1 cut(s) 952
EcoRII CCWGG 2 cut(s) 435, 721
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 2 cut(s) 426, 665
ErhI CCWWGG 2 cut(s) 426, 665
FaeI CATG 5 cut(s) 269, 493, 669, 758, 797
FatI CATG 5 cut(s) 265, 489, 665, 754, 793
FauI CCCGC 1 cut(s) 338
FauNDI CATATG 2 cut(s) 823, 1100
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 2 cut(s) 419, 1026
FokI GGATG 5 cut(s) 407, 578, 874, 884, 1132
Fsp4HI GCNGC 2 cut(s) 419, 1026
FspBI CTAG 2 cut(s) 674, 1157
GlaI GCGC 1 cut(s) 750
GluI GCNGC 2 cut(s) 419, 1026
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 2 cut(s) 182, 870
HapII CCGG 2 cut(s) 234, 1148
HgaI GACGC 1 cut(s) 530
HhaI GCGC 1 cut(s) 751
Hin1II CATG 5 cut(s) 269, 493, 669, 758, 797
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 5 cut(s) 11, 37, 121, 670, 940
HpaII CCGG 2 cut(s) 234, 1148
HphI GGTGA 3 cut(s) 338, 401, 803
Hpy166II GTNNAC 6 cut(s) 293, 339, 520, 601, 1078, 1214
Hpy188I TCNGA 4 cut(s) 36, 322, 967, 1190
Hpy188III TCNNGA 6 cut(s) 482, 893, 954, 1007, 1143, 1157
Hpy8I GTNNAC 6 cut(s) 293, 339, 520, 601, 1078, 1214
HpyAV CCTTC 5 cut(s) 105, 138, 159, 443, 722
HpyCH4III ACNGT 6 cut(s) 164, 355, 572, 929, 1069, 1082
HpyCH4IV ACGT 2 cut(s) 816, 1113
HpyCH4V TGCA 3 cut(s) 265, 293, 821
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 755, 1031
HpyF3I CTNAG 8 cut(s) 35, 99, 115, 716, 872, 964, 1035, 1217
HpySE526I ACGT 2 cut(s) 816, 1113
Hsp92II CATG 5 cut(s) 269, 493, 669, 758, 797
HspAI GCGC 1 cut(s) 749
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
LguI GCTCTTC 1 cut(s) 791
LmnI GCTCC 2 cut(s) 235, 455
Lsp1109I GCAGC 2 cut(s) 405, 1037
MaeI CTAG 2 cut(s) 674, 1157
MaeII ACGT 2 cut(s) 816, 1113
MaeIII GTNAC 3 cut(s) 4, 164, 986
MalI GATC 7 cut(s) 261, 279, 384, 424, 663, 706, 891
MboI GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
MboII GAAGA 7 cut(s) 129, 132, 144, 341, 599, 644, 778
MfeI CAATTG 1 cut(s) 1090
MflI RGATCY 2 cut(s) 259, 661
MhlI GDGCHC 2 cut(s) 34, 295
MluCI AATT 4 cut(s) 243, 399, 1090, 1138
MlyI GAGTC 2 cut(s) 46, 115
MseI TTAA 2 cut(s) 768, 1137
MslI CAYNNNNRTG 2 cut(s) 494, 736
MspA1I CMGCKG 1 cut(s) 1028
MspCI CTTAAG 1 cut(s) 767
MspI CCGG 2 cut(s) 234, 1148
MspR9I CCNGG 3 cut(s) 234, 437, 723
MunI CAATTG 1 cut(s) 1090
MvaI CCWGG 2 cut(s) 437, 723
MvnI CGCG 1 cut(s) 751
MwoI GCNNNNNNNGC 3 cut(s) 109, 755, 1031
NciI CCSGG 1 cut(s) 234
NcoI CCATGG 1 cut(s) 665
NdeI CATATG 2 cut(s) 823, 1100
NdeII GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
NlaIII CATG 5 cut(s) 269, 493, 669, 758, 797
NlaIV GGNNCC 1 cut(s) 663
NmuCI GTSAC 1 cut(s) 4
NspI RCATGY 1 cut(s) 758
PaeR7I CTCGAG 1 cut(s) 952
PceI AGGCCT 2 cut(s) 182, 870
PciSI GCTCTTC 1 cut(s) 791
PfeI GAWTC 3 cut(s) 11, 670, 940
PkrI GCNGC 2 cut(s) 420, 1027
PleI GAGTC 2 cut(s) 45, 115
PpsI GAGTC 2 cut(s) 45, 115
Psp6I CCWGG 2 cut(s) 435, 721
PspGI CCWGG 2 cut(s) 435, 721
PspN4I GGNNCC 1 cut(s) 663
PspPI GGNCC 1 cut(s) 728
PsuI RGATCY 2 cut(s) 259, 661
PvuII CAGCTG 1 cut(s) 1028
RsaI GTAC 1 cut(s) 950
RsaNI GTAC 1 cut(s) 949
RseI CAYNNNNRTG 2 cut(s) 494, 736
SapI GCTCTTC 1 cut(s) 791
SaqAI TTAA 2 cut(s) 768, 1137
SatI GCNGC 2 cut(s) 419, 1026
Sau3AI GATC 7 cut(s) 259, 277, 382, 422, 661, 704, 889
Sau96I GGNCC 1 cut(s) 728
SchI GAGTC 2 cut(s) 46, 115
ScrFI CCNGG 3 cut(s) 234, 437, 723
SduI GDGCHC 2 cut(s) 34, 295
SfcI CTRYAG 2 cut(s) 571, 1068
Sfr274I CTCGAG 1 cut(s) 952
SinI GGWCC 1 cut(s) 728
SlaI CTCGAG 1 cut(s) 952
SmiMI CAYNNNNRTG 2 cut(s) 494, 736
SmlI CTYRAG 3 cut(s) 296, 767, 952
SmoI CTYRAG 3 cut(s) 296, 767, 952
Sse9I AATT 4 cut(s) 243, 399, 1090, 1138
SseBI AGGCCT 2 cut(s) 182, 870
SsiI CCGC 3 cut(s) 345, 726, 1105
SspMI CTAG 2 cut(s) 674, 1157
StuI AGGCCT 2 cut(s) 182, 870
StyD4I CCNGG 3 cut(s) 232, 435, 721
StyI CCWWGG 2 cut(s) 426, 665
TaaI ACNGT 6 cut(s) 164, 355, 572, 929, 1069, 1082
TaiI ACGT 2 cut(s) 819, 1116
TaqI TCGA 4 cut(s) 385, 511, 953, 972
TasI AATT 4 cut(s) 243, 399, 1090, 1138
TatI WGTACW 1 cut(s) 948
TfiI GAWTC 3 cut(s) 11, 670, 940
Tru1I TTAA 2 cut(s) 768, 1137
Tru9I TTAA 2 cut(s) 768, 1137
TscAI CASTG 7 cut(s) 13, 162, 208, 880, 934, 1216, 1225
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 2 cut(s) 418, 1025
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 5 cut(s) 132, 411, 506, 854, 895
TspRI CASTG 7 cut(s) 13, 162, 208, 880, 934, 1216, 1225
Vha464I CTTAAG 1 cut(s) 767
VneI GTGCAC 1 cut(s) 291
VpaK11BI GGWCC 1 cut(s) 728
XbaI TCTAGA 1 cut(s) 1156
XceI RCATGY 1 cut(s) 758
XcmI CCANNNNNNNNNTGG 2 cut(s) 52, 821
XhoI CTCGAG 1 cut(s) 952
XspI CTAG 2 cut(s) 674, 1157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.