Rroxscaffold_1G00018960

trans-2-enoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011303
Physical Location & Seq
Forward (+)
23065201 .. 23068196
2996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00018960.1

Sequence Viewer

Length: 1176 bp
ATGGCGTCGCCGGTTCGATCACCGGCTCTCAGAGCTCTGAAACCACTCTTCAATTTCACTCTCCACGACACCTTAAACCCTAGACCCCGAACCCTAAGCCTTCGGGCCTTCTCCACCACCTTGTCTCCGCCGTCTAAGGCGGTCGTCTACGAGCAACACGGCCCTCCCGATAGCGTTACCAGAGTGATAAATTTGCCGCCGGTTGAAGTGAAAGAGAACGATGTCTGTGTCAGAATGCTGGCTTCTCCTATTAACCCTTCTGATATTAACAGGATTGAAGGGGTGTACCCTGTGAGGCCACAAGTGCCAGCGGTTGGAGGATATGAAGGTGTGGGAGAGGTACACTCTGTCGGCTCTGCGGTTAAAGGACTATCGCCTGGAGATTTGGTCATCGCATCTCCACCTTCTTCCGGGATGTGGCAAACTCATGTTGTGAAAGATCAGGGTGTATGGTACAAGATTAATAAAGATTCACCCTTGGAATATGCTGCTACCATTACTGTAAATCCATTGACTGCTCTAAGAATGCTTGAAGACTTCACCACTTTAAAGTCAGGAGATGCTGTTGTTCAAAATGGAGCCACCAGCATCGTGGGGCAGTGCATTATTCAGCTTGCAAAGAATCGTGGCATTCATAGCGTTAATATTATAAGAGACAGGGCTGGCTCAGATGAAGTAAAGGAAAAGCTTAAAAAACTTGGTGCTGATGAAGTTTTTACTGAGAGCGAACTGCAAGTCAAGGATGTCAAGGGTCTTCTGTCTGGCATACCTGAACCTGCATTGGGATTTAACTGTGTCGGAGGCAATGCTGCTTCCTTGGTTCTTAAATTTTTGGGTCGTGGCGGCACTATGGTGACTTATGGTGGCATGTCTAAGAAACCAATTACTGTATCAACTTCATATTTCATTTTTAAGGATCTTTCCTTGAGGGGATTCTGGTTGCAGAGTTGGATGAGTACAGAGAAGGCAGCAGAGTGCAGAGTTCTGATAGATCACCTCCTTGACTTAGCACGGGAAGGGAAATTAAAATATGAGATGGAGCTGGTTCCTTTCAACAGTTTCCACACTGCACTAGACAAAGCACTTGGGAAACAAGGGAGCCAACCGAAGCAAAGAAATGATGCTCTGCTTATGAGCTATACCATGGCATCTGGGAAGCAATCACGTTCTCCATGA

Protein Analysis

391

Amino Acids

42.44

Weight (kDa)

9.18

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 72 - 134 8.3e-12 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 198 - 324 5.1e-16 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45770 AT3G45770
fragaria_vesca FvH4_3g34120
malus_domestica MD03G1107400.v1.1
prunus_persica Prupe.6G090200_v2.0.a1 Prupe.6G090200_v2.0.a1 Prupe.6G090200_v2.0.a1
pyrus_communis pycom03g08690
rosa_chinensis RchiOBHm_Chr5g0061771
rosa_laevigata RLG00000035504
rosa_multiflora Rmu_sc0003709.1_g000010
rosa_roxburghii Rroxscaffold_1G00018960
rosa_rugosa Rorug05G0343400 Rorug05G0343500 Rorug05G0343600
rosa_samantha Rh5AG404500 Rh5BG417600 Rh5CG442000 Rh5DG431700
rosa_wichuraiana Rw5G038000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 650
Acc36I ACCTGC 1 cut(s) 784
AccB7I CCANNNNNTGG 1 cut(s) 314
AccI GTMKAC 1 cut(s) 147
AciI CCGC 6 cut(s) 128, 140, 197, 311, 359, 843
AclWI GGATC 1 cut(s) 924
AcsI RAATTY 2 cut(s) 190, 827
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 287, 342, 455, 958
AfiI CCNNNNNNNGG 4 cut(s) 314, 410, 417, 782
AgsI TTSAA 6 cut(s) 52, 206, 278, 533, 572, 1054
AjnI CCWGG 1 cut(s) 376
AleI CACNNNNGTG 1 cut(s) 851
AluBI AGCT 5 cut(s) 35, 613, 688, 1042, 1137
AluI AGCT 5 cut(s) 35, 613, 688, 1042, 1137
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 2 cut(s) 129, 648
AlwI GGATC 1 cut(s) 924
AoxI GGCC 3 cut(s) 105, 160, 296
ApeKI GCWGC 3 cut(s) 488, 809, 968
ApoI RAATTY 2 cut(s) 190, 827
ArsI GACNNNNNNTTYG 2 cut(s) 720, 752
AseI ATTAAT 1 cut(s) 462
AspS9I GGNCC 2 cut(s) 105, 161
AsuC2I CCSGG 1 cut(s) 412
AsuHPI GGTGA 5 cut(s) 12, 465, 532, 865, 986
BanII GRGCYC 1 cut(s) 37
BbsI GAAGAC 2 cut(s) 540, 746
Bbv12I GWGCWC 1 cut(s) 37
BbvI GCAGC 3 cut(s) 475, 796, 980
BccI CCATC 1 cut(s) 1030
BceAI ACGGC 2 cut(s) 115, 175
BciT130I CCWGG 1 cut(s) 378
BcnI CCSGG 1 cut(s) 412
BcoDI GTCTC 2 cut(s) 129, 648
BfaI CTAG 2 cut(s) 81, 1073
BfuAI ACCTGC 1 cut(s) 784
BisI GCNGC 5 cut(s) 197, 489, 810, 844, 969
BlsI GCNGC 5 cut(s) 198, 490, 811, 845, 970
Bme1390I CCNGG 2 cut(s) 378, 412
BmgT120I GGNCC 2 cut(s) 105, 161
BmiI GGNNCC 3 cut(s) 580, 1047, 1100
BmrFI CCNGG 2 cut(s) 378, 412
BmsI GCATC 5 cut(s) 404, 550, 597, 1111, 1157
BpiI GAAGAC 2 cut(s) 540, 746
BplI GAGNNNNNCTC 2 cut(s) 329, 361
BpmI CTGGAG 1 cut(s) 399
Bpu10I CCTNAGC 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 946
BpuMI CCSGG 1 cut(s) 412
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 477, 816, 1143
BsaXI ACNNNNNCTCC 6 cut(s) 109, 139, 372, 402, 549, 579
Bsc4I CCNNNNNNNGG 4 cut(s) 314, 410, 417, 782
Bse118I RCCGGY 3 cut(s) 10, 22, 199
Bse3DI GCAATG 1 cut(s) 811
BseBI CCWGG 1 cut(s) 378
BseDI CCNNGG 3 cut(s) 477, 816, 1143
BseGI GGATG 3 cut(s) 420, 748, 957
BseLI CCNNNNNNNGG 4 cut(s) 314, 410, 417, 782
BseMI GCAATG 1 cut(s) 811
BseMII CTCAG 3 cut(s) 43, 681, 711
BseXI GCAGC 3 cut(s) 475, 796, 980
BsgI GTGCAG 2 cut(s) 997, 1053
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 3 cut(s) 107, 162, 298
BsiEI CGRYCG 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 37
BsiSI CCGG 4 cut(s) 11, 23, 200, 411
BslI CCNNNNNNNGG 4 cut(s) 314, 410, 417, 782
BsmAI GTCTC 2 cut(s) 129, 648
BsmI GAATGC 3 cut(s) 240, 531, 630
BsnI GGCC 3 cut(s) 107, 162, 298
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 4 cut(s) 17, 439, 916, 991
Bsp19I CCATGG 1 cut(s) 1143
BspACI CCGC 6 cut(s) 128, 140, 197, 311, 359, 843
BspANI GGCC 3 cut(s) 107, 162, 298
BspCNI CTCAG 3 cut(s) 42, 680, 712
BspLI GGNNCC 3 cut(s) 580, 1047, 1100
BspMI ACCTGC 1 cut(s) 784
BspPI GGATC 1 cut(s) 924
BsrDI GCAATG 1 cut(s) 811
BsrFI RCCGGY 3 cut(s) 10, 22, 199
BssAI RCCGGY 3 cut(s) 10, 22, 199
BssECI CCNNGG 3 cut(s) 477, 816, 1143
BssMI GATC 4 cut(s) 17, 439, 916, 991
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 477, 816, 1143
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 4 cut(s) 502, 794, 889, 1058
Bst6I CTCTTC 1 cut(s) 53
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 4 cut(s) 240, 309, 615, 664
BstDEI CTNAG 8 cut(s) 29, 95, 135, 521, 667, 720, 873, 1006
BstDSI CCRYGG 1 cut(s) 1143
BstF5I GGATG 3 cut(s) 420, 748, 957
BstKTI GATC 4 cut(s) 20, 442, 919, 994
BstMAI GTCTC 2 cut(s) 129, 648
BstMBI GATC 4 cut(s) 17, 439, 916, 991
BstMCI CGRYCG 1 cut(s) 144
BstMWI GCNNNNNNNGC 3 cut(s) 32, 304, 636
BstNI CCWGG 1 cut(s) 378
BstNSI RCATGY 1 cut(s) 871
BstSCI CCNGG 2 cut(s) 376, 410
BstV1I GCAGC 3 cut(s) 475, 796, 980
BstV2I GAAGAC 2 cut(s) 540, 746
BstX2I RGATCY 1 cut(s) 916
BstXI CCANNNNNNTGG 1 cut(s) 592
BstYI RGATCY 1 cut(s) 916
BsuRI GGCC 3 cut(s) 107, 162, 298
BtgI CCRYGG 1 cut(s) 1143
BtgZI GCGATG 1 cut(s) 376
BtsCI GGATG 3 cut(s) 420, 748, 957
BtsI GCAGTG 2 cut(s) 605, 1065
BtsIMutI CAGTG 2 cut(s) 605, 1065
BveI ACCTGC 1 cut(s) 784
Cac8I GCNNGC 4 cut(s) 240, 309, 615, 664
Cfr10I RCCGGY 3 cut(s) 10, 22, 199
Cfr13I GGNCC 2 cut(s) 105, 161
Csp6I GTAC 4 cut(s) 286, 341, 454, 957
CviAII CATG 4 cut(s) 428, 868, 1144, 1173
CviQI GTAC 4 cut(s) 286, 341, 454, 957
DdeI CTNAG 8 cut(s) 29, 95, 135, 521, 667, 720, 873, 1006
DpnI GATC 4 cut(s) 19, 441, 918, 993
DpnII GATC 4 cut(s) 17, 439, 916, 991
DraI TTTAAA 1 cut(s) 549
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
EciI GGCGGA 1 cut(s) 117
Ecl136II GAGCTC 1 cut(s) 35
Eco130I CCWWGG 3 cut(s) 477, 816, 1143
Eco24I GRGCYC 1 cut(s) 37
Eco53kI GAGCTC 1 cut(s) 35
EcoICRI GAGCTC 1 cut(s) 35
EcoRII CCWGG 1 cut(s) 376
EcoT14I CCWWGG 3 cut(s) 477, 816, 1143
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 3 cut(s) 477, 816, 1143
FaeI CATG 4 cut(s) 431, 871, 1147, 1176
FatI CATG 4 cut(s) 427, 867, 1143, 1172
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 5 cut(s) 197, 489, 810, 844, 969
FokI GGATG 3 cut(s) 427, 755, 964
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 5 cut(s) 197, 489, 810, 844, 969
FspBI CTAG 2 cut(s) 81, 1073
GluI GCNGC 5 cut(s) 197, 489, 810, 844, 969
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 3 cut(s) 107, 162, 298
HapII CCGG 4 cut(s) 11, 23, 200, 411
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 431, 871, 1147, 1176
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 3 cut(s) 470, 622, 933
HpaII CCGG 4 cut(s) 11, 23, 200, 411
HphI GGTGA 5 cut(s) 12, 465, 532, 865, 986
Hpy166II GTNNAC 3 cut(s) 148, 286, 343
Hpy188I TCNGA 7 cut(s) 32, 39, 233, 262, 670, 800, 987
Hpy188III TCNNGA 2 cut(s) 167, 555
Hpy8I GTNNAC 3 cut(s) 148, 286, 343
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 8 cut(s) 110, 118, 267, 272, 320, 414, 958, 1010
HpyCH4III ACNGT 4 cut(s) 502, 794, 889, 1058
HpyCH4IV ACGT 1 cut(s) 1165
HpyCH4V TGCA 7 cut(s) 603, 617, 733, 779, 943, 978, 1070
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 304, 636
HpyF3I CTNAG 8 cut(s) 29, 95, 135, 521, 667, 720, 873, 1006
HpySE526I ACGT 1 cut(s) 1165
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 431, 871, 1147, 1176
Kzo9I GATC 4 cut(s) 17, 439, 916, 991
LmnI GCTCC 3 cut(s) 578, 1039, 1098
Lsp1109I GCAGC 3 cut(s) 475, 796, 980
LweI GCATC 5 cut(s) 404, 550, 597, 1111, 1157
MaeI CTAG 2 cut(s) 81, 1073
MaeII ACGT 1 cut(s) 1165
MaeIII GTNAC 2 cut(s) 175, 853
MalI GATC 4 cut(s) 19, 441, 918, 993
MboI GATC 4 cut(s) 17, 439, 916, 991
MboII GAAGA 4 cut(s) 40, 399, 545, 746
MflI RGATCY 1 cut(s) 916
MhlI GDGCHC 1 cut(s) 37
MluCI AATT 5 cut(s) 52, 190, 827, 882, 1022
MmeI TCCRAC 3 cut(s) 295, 778, 929
MnlI CCTC 7 cut(s) 174, 288, 311, 331, 794, 921, 1007
MslI CAYNNNNRTG 1 cut(s) 851
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 4 cut(s) 11, 23, 200, 411
MspR9I CCNGG 2 cut(s) 378, 412
Mva1269I GAATGC 3 cut(s) 240, 531, 630
MvaI CCWGG 1 cut(s) 378
MwoI GCNNNNNNNGC 3 cut(s) 32, 304, 636
NciI CCSGG 1 cut(s) 412
NcoI CCATGG 1 cut(s) 1143
NdeII GATC 4 cut(s) 17, 439, 916, 991
NlaIII CATG 4 cut(s) 431, 871, 1147, 1176
NlaIV GGNNCC 3 cut(s) 580, 1047, 1100
NmuCI GTSAC 1 cut(s) 853
NspI RCATGY 1 cut(s) 871
OliI CACNNNNGTG 1 cut(s) 851
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 3 cut(s) 240, 531, 630
PfeI GAWTC 3 cut(s) 470, 622, 933
PflMI CCANNNNNTGG 1 cut(s) 314
PfoI TCCNGGA 1 cut(s) 410
PkrI GCNGC 5 cut(s) 198, 490, 811, 845, 970
PshBI ATTAAT 1 cut(s) 462
PsiI TTATAA 1 cut(s) 650
Psp124BI GAGCTC 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 3 cut(s) 580, 1047, 1100
PspPI GGNCC 2 cut(s) 105, 161
PsuI RGATCY 1 cut(s) 916
RsaI GTAC 4 cut(s) 287, 342, 455, 958
RsaNI GTAC 4 cut(s) 286, 341, 454, 957
RseI CAYNNNNRTG 1 cut(s) 851
SacI GAGCTC 1 cut(s) 37
SatI GCNGC 5 cut(s) 197, 489, 810, 844, 969
Sau3AI GATC 4 cut(s) 17, 439, 916, 991
Sau96I GGNCC 2 cut(s) 105, 161
ScrFI CCNGG 2 cut(s) 378, 412
SduI GDGCHC 1 cut(s) 37
SfaNI GCATC 5 cut(s) 404, 550, 597, 1111, 1157
SmiMI CAYNNNNRTG 1 cut(s) 851
SmlI CTYRAG 1 cut(s) 925
SmoI CTYRAG 1 cut(s) 925
Sse9I AATT 5 cut(s) 52, 190, 827, 882, 1022
SsiI CCGC 6 cut(s) 128, 140, 197, 311, 359, 843
SspI AATATT 1 cut(s) 646
SspMI CTAG 2 cut(s) 81, 1073
SstI GAGCTC 1 cut(s) 37
StyD4I CCNGG 2 cut(s) 376, 410
StyI CCWWGG 3 cut(s) 477, 816, 1143
TaaI ACNGT 4 cut(s) 502, 794, 889, 1058
TaiI ACGT 1 cut(s) 1168
TaqI TCGA 1 cut(s) 16
TasI AATT 5 cut(s) 52, 190, 827, 882, 1022
TatI WGTACW 1 cut(s) 956
TauI GCSGC 2 cut(s) 199, 846
TfiI GAWTC 3 cut(s) 470, 622, 933
TscAI CASTG 2 cut(s) 605, 1072
TseFI GTSAC 1 cut(s) 853
TseI GCWGC 3 cut(s) 488, 809, 968
Tsp45I GTSAC 1 cut(s) 853
TspDTI ATGAA 6 cut(s) 339, 623, 687, 723, 888, 895
TspRI CASTG 2 cut(s) 605, 1072
Van91I CCANNNNNTGG 1 cut(s) 314
VspI ATTAAT 1 cut(s) 462
XapI RAATTY 2 cut(s) 190, 827
XceI RCATGY 1 cut(s) 871
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 2 cut(s) 81, 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.