Rroxscaffold_2G00084770

RING-type zinc-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009032
Physical Location & Seq
Forward (+)
7268982 .. 7269781
800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00084770.1

Sequence Viewer

Length: 711 bp
ATGTGGAATTTTGCATCCAGTTGCATTGCTGGAAATGTTGGAATGAAAAATGACTTTCTAAGGCCAACACAAGCTACTTCTGAATGCTGCTCCGATGATGAGGGTTCTTCTGGTGTTGGCAGAGAGGAAGGACTAGAGTGCCCCATATGCTGGGAATCTTTCAATATTGTTGAAAATGTGCCCTATGTTTTATGGTGTGGTCATACCCTCTGTAAAAATTGCATTCTGGGTCTGCAATGGGCTGGAAATCTCAAATTTCCTCGCAAGAACTACTTTCTTTTGTGGATGGTTGAAAGCATGAATGGAGATAGAGTGAAGTCTCATTCTACCTGCTCTGGTGATAATCCACCAGCCTGGCCGGTAAATGGAAATGTATCCGCAGGAAGTCAACTGAACCATAGGAGGGGACAATATATTCGCCATCCCGAGACACCAGGGACAAATCATAATCATGGCTTCGTCAATAATTACCTTAGTATGGAGCGATTGCATTCTTCCCTTTGGAAGTCACTGTTTTTCTTCGTTCATTTGACAGCTAAGTTCCCTCTGGTTGTCATATTTCTTCTGATCATCTTATATGCAATACCTGCCAGTGCAGCCATATTGGCCTTGTACATACTTATCACGGTGCTGTTTGCTCTCCCATCGTTTCTTATCTTGTACTTTGCATATCCTAGTTTGGATTGGCTTGTGAGAGAAATCATCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_UBOX

Protein Analysis

236

Amino Acids

26.6

Weight (kDa)

6.69

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_5 PF14634 46 - 78 7.4e-07 zinc-RING finger domain
zf-RING_2 PF13639 46 - 76 7.3e-06 Ring finger domain
zf-RING_UBOX PF13445 47 - 80 1.3e-09 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 338, 595
AccB7I CCANNNNNTGG 1 cut(s) 150
AciI CCGC 1 cut(s) 378
AcoI YGGCCR 1 cut(s) 356
AcsI RAATTY 2 cut(s) 7, 254
AfaI GTAC 2 cut(s) 614, 662
AfiI CCNNNNNNNGG 4 cut(s) 150, 365, 403, 478
AgsI TTSAA 3 cut(s) 163, 173, 293
AjnI CCWGG 2 cut(s) 353, 433
AluBI AGCT 2 cut(s) 74, 536
AluI AGCT 2 cut(s) 74, 536
Alw26I GTCTC 2 cut(s) 324, 422
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 3 cut(s) 62, 356, 606
ApeKI GCWGC 2 cut(s) 87, 596
ApoI RAATTY 2 cut(s) 7, 254
ArsI GACNNNNNNTTYG 2 cut(s) 399, 431
AsuHPI GGTGA 2 cut(s) 350, 697
AvaI CYCGRG 1 cut(s) 425
BaeGI GKGCMC 2 cut(s) 143, 183
BbvI GCAGC 2 cut(s) 74, 608
BccI CCATC 3 cut(s) 280, 429, 652
BciT130I CCWGG 2 cut(s) 355, 435
BciVI GTATCC 1 cut(s) 385
BclI TGATCA 1 cut(s) 567
BcoDI GTCTC 2 cut(s) 324, 422
BfaI CTAG 2 cut(s) 134, 675
BfuAI ACCTGC 2 cut(s) 338, 595
BfuI GTATCC 1 cut(s) 385
BglI GCCNNNNNGGC 1 cut(s) 605
BisI GCNGC 2 cut(s) 88, 597
BlsI GCNGC 2 cut(s) 89, 598
Bme1390I CCNGG 2 cut(s) 355, 435
BmeT110I CYCGRG 1 cut(s) 425
BmrFI CCNGG 2 cut(s) 355, 435
BmsI GCATC 1 cut(s) 23
BsaJI CCNNGG 1 cut(s) 434
BsaXI ACNNNNNCTCC 2 cut(s) 297, 327
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 365, 403, 478
Bse118I RCCGGY 1 cut(s) 358
Bse1I ACTGG 2 cut(s) 18, 591
Bse3DI GCAATG 2 cut(s) 24, 242
BseBI CCWGG 2 cut(s) 355, 435
BseDI CCNNGG 1 cut(s) 434
BseGI GGATG 3 cut(s) 14, 291, 421
BseLI CCNNNNNNNGG 4 cut(s) 150, 365, 403, 478
BseMI GCAATG 2 cut(s) 24, 242
BseNI ACTGG 2 cut(s) 18, 591
BseSI GKGCMC 2 cut(s) 143, 183
BseXI GCAGC 2 cut(s) 74, 608
BseYI CCCAGC 1 cut(s) 150
BsgI GTGCAG 1 cut(s) 615
BshFI GGCC 3 cut(s) 64, 358, 608
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 1 cut(s) 359
BslFI GGGAC 2 cut(s) 420, 451
BslI CCNNNNNNNGG 4 cut(s) 150, 365, 403, 478
BsmAI GTCTC 2 cut(s) 324, 422
BsmFI GGGAC 2 cut(s) 420, 451
BsmI GAATGC 3 cut(s) 89, 222, 490
BsnI GGCC 3 cut(s) 64, 358, 608
BsoBI CYCGRG 1 cut(s) 425
Bsp1286I GDGCHC 2 cut(s) 143, 183
Bsp1407I TGTACA 1 cut(s) 612
Bsp143I GATC 1 cut(s) 567
BspACI CCGC 1 cut(s) 378
BspANI GGCC 3 cut(s) 64, 358, 608
BspMI ACCTGC 2 cut(s) 338, 595
BsrDI GCAATG 2 cut(s) 24, 242
BsrFI RCCGGY 1 cut(s) 358
BsrGI TGTACA 1 cut(s) 612
BsrI ACTGG 2 cut(s) 18, 591
BssAI RCCGGY 1 cut(s) 358
BssECI CCNNGG 1 cut(s) 434
BssMI GATC 1 cut(s) 567
Bst2UI CCWGG 2 cut(s) 355, 435
Bst4CI ACNGT 2 cut(s) 513, 628
BstAPI GCANNNNNTGC 1 cut(s) 587
BstAUI TGTACA 1 cut(s) 612
BstDEI CTNAG 3 cut(s) 59, 473, 537
BstF5I GGATG 3 cut(s) 14, 291, 421
BstKTI GATC 1 cut(s) 570
BstMAI GTCTC 2 cut(s) 324, 422
BstMBI GATC 1 cut(s) 567
BstMWI GCNNNNNNNGC 4 cut(s) 147, 587, 596, 605
BstNI CCWGG 2 cut(s) 355, 435
BstSCI CCNGG 2 cut(s) 353, 433
BstSLI GKGCMC 2 cut(s) 143, 183
BstV1I GCAGC 2 cut(s) 74, 608
BstXI CCANNNNNNTGG 1 cut(s) 354
BsuI GTATCC 1 cut(s) 385
BsuRI GGCC 3 cut(s) 64, 358, 608
BtsCI GGATG 3 cut(s) 14, 291, 421
BtsIMutI CAGTG 2 cut(s) 509, 598
BveI ACCTGC 2 cut(s) 338, 595
Cfr10I RCCGGY 1 cut(s) 358
Csp6I GTAC 2 cut(s) 613, 661
CviAII CATG 2 cut(s) 298, 452
CviQI GTAC 2 cut(s) 613, 661
DdeI CTNAG 3 cut(s) 59, 473, 537
DpnI GATC 1 cut(s) 569
DpnII GATC 1 cut(s) 567
EaeI YGGCCR 1 cut(s) 356
Eco88I CYCGRG 1 cut(s) 425
EcoRII CCWGG 2 cut(s) 353, 433
FaeI CATG 2 cut(s) 301, 455
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 2 cut(s) 420, 451
FatI CATG 2 cut(s) 297, 451
FauNDI CATATG 1 cut(s) 146
FbaI TGATCA 1 cut(s) 567
Fnu4HI GCNGC 2 cut(s) 88, 597
FokI GGATG 2 cut(s) 298, 408
Fsp4HI GCNGC 2 cut(s) 88, 597
FspBI CTAG 2 cut(s) 134, 675
GluI GCNGC 2 cut(s) 88, 597
GsaI CCCAGC 1 cut(s) 154
HaeIII GGCC 3 cut(s) 64, 358, 608
HapII CCGG 1 cut(s) 359
Hin1II CATG 2 cut(s) 301, 455
HincII GTYRAC 1 cut(s) 389
HindII GTYRAC 1 cut(s) 389
HinfI GANTC 1 cut(s) 155
HpaII CCGG 1 cut(s) 359
HphI GGTGA 2 cut(s) 350, 697
Hpy166II GTNNAC 1 cut(s) 389
Hpy188I TCNGA 3 cut(s) 82, 94, 567
Hpy188III TCNNGA 1 cut(s) 425
Hpy8I GTNNAC 1 cut(s) 389
HpyAV CCTTC 1 cut(s) 122
HpyCH4III ACNGT 2 cut(s) 513, 628
HpyCH4V TGCA 8 cut(s) 14, 24, 222, 235, 490, 581, 596, 668
HpyF10VI GCNNNNNNNGC 4 cut(s) 147, 587, 596, 605
HpyF3I CTNAG 3 cut(s) 59, 473, 537
Hsp92II CATG 2 cut(s) 301, 455
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 1 cut(s) 567
LmnI GCTCC 2 cut(s) 95, 481
Lsp1109I GCAGC 2 cut(s) 74, 608
LweI GCATC 1 cut(s) 23
MaeI CTAG 2 cut(s) 134, 675
MaeIII GTNAC 1 cut(s) 507
MalI GATC 1 cut(s) 569
MboI GATC 1 cut(s) 567
MboII GAAGA 4 cut(s) 99, 486, 511, 554
MhlI GDGCHC 2 cut(s) 143, 183
MluCI AATT 4 cut(s) 7, 217, 254, 466
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 6 cut(s) 94, 118, 218, 270, 396, 555
MslI CAYNNNNRTG 1 cut(s) 450
MspI CCGG 1 cut(s) 359
MspR9I CCNGG 2 cut(s) 355, 435
Mva1269I GAATGC 3 cut(s) 89, 222, 490
MvaI CCWGG 2 cut(s) 355, 435
MwoI GCNNNNNNNGC 4 cut(s) 147, 587, 596, 605
NdeI CATATG 1 cut(s) 146
NdeII GATC 1 cut(s) 567
NlaIII CATG 2 cut(s) 301, 455
NmuCI GTSAC 1 cut(s) 507
PctI GAATGC 3 cut(s) 89, 222, 490
PfeI GAWTC 1 cut(s) 155
PflMI CCANNNNNTGG 1 cut(s) 150
PkrI GCNGC 2 cut(s) 89, 598
Psp6I CCWGG 2 cut(s) 353, 433
PspFI CCCAGC 1 cut(s) 150
PspGI CCWGG 2 cut(s) 353, 433
RsaI GTAC 2 cut(s) 614, 662
RsaNI GTAC 2 cut(s) 613, 661
RseI CAYNNNNRTG 1 cut(s) 450
SatI GCNGC 2 cut(s) 88, 597
Sau3AI GATC 1 cut(s) 567
ScrFI CCNGG 2 cut(s) 355, 435
SduI GDGCHC 2 cut(s) 143, 183
SetI ASST 6 cut(s) 76, 332, 474, 538, 589, 710
SfaNI GCATC 1 cut(s) 23
SmiMI CAYNNNNRTG 1 cut(s) 450
Sse9I AATT 4 cut(s) 7, 217, 254, 466
SsiI CCGC 1 cut(s) 378
SspI AATATT 1 cut(s) 166
SspMI CTAG 2 cut(s) 134, 675
StyD4I CCNGG 2 cut(s) 353, 433
TaaI ACNGT 2 cut(s) 513, 628
TasI AATT 4 cut(s) 7, 217, 254, 466
TatI WGTACW 2 cut(s) 612, 660
TfiI GAWTC 1 cut(s) 155
TscAI CASTG 2 cut(s) 516, 598
TseFI GTSAC 1 cut(s) 507
TseI GCWGC 2 cut(s) 87, 596
Tsp45I GTSAC 1 cut(s) 507
TspDTI ATGAA 3 cut(s) 59, 314, 515
TspRI CASTG 2 cut(s) 516, 598
Van91I CCANNNNNTGG 1 cut(s) 150
XapI RAATTY 2 cut(s) 7, 254
XspI CTAG 2 cut(s) 134, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.