Rroxscaffold_2G00095950

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018463
Physical Location & Seq
Forward (+)
17253023 .. 17254465
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00095950.1

Sequence Viewer

Length: 1443 bp
ATGGCACAGCAGCAGATTAAGACCCGGCATCGTCCTGAATATGAGGTCCAGGATATTATTGTTGAGATACTGTCGAGGCTACCAGTTAAGTCTTTGAAGCGATTCCAGTGCGTATGCAAGTCGTGGTGTGATTTAATCTCCGATTCCTTTTTTATTATGAAGCATGCCAATCAAGCAAAGAAAGACACCAAAAGCTTGAAGTTTCTTGTTTCAATCGAGCCTCCCCAATCCGTAGACTACGAAGCTTTACTGAAGCATGAAGATGATGGACATGTTGCAAGCAGGCTACTTGAGCTGGATTTATCAAAGTGCTGCCCATCCATATATGTTGAGGCTTGGTCTTGTCATCTTTACGTTGTGGGATCTTGTAATGGGTTGATATGTCTACATGGAGTCGACATGAATCGAGACTGCAGTGACATTCTTTTATGGAATCCTTGTACTGGAGATACCTGTGTGTTACCGGAACATACTAATTTATTTCGAGCTCATTATGGATATCGTCATCCGATGTTTTATGGATTCGGTTATGATTCCACCACTGAAGATTACAAGGTCATACTAGGGGACAGCTATATATGTGAGTACGATGATGAAGGTGCCTTTAAGATTCTCAACAAAGTTGCTGTCTTTACACTTAAAACAGGTTCATGGAGGAACTATTATGAAGAAAGGAGGGTGAATCGTAGCAATGAAAGACGGAGCATTAGATTGGCTGGACAAGGGTGCTTATCAAACGGAGCTCTACATTGGATGGAACTTGTGTTCGACTATAGATACTGCCTAAATGGTTCGAGCTCAAGGATTATGTCTTTTGATTTAGCTAGGGAGAAATTTCAAGAGATGGTGTCACTATCCAGTTTTGTCGACAAAGAATGTTTGGACCACATTTCCGCCTCCTACGGAACCTTAGTCGGAACCACGATCAGACCTTGTGCCGGAATTGGGAGTACTATTGAAAACAATCTTCTTGTATACTTTTACGTTCCTAGCATTAATACCAGTGATACTACGTTTACAATATGGGTGATGAACGAATATGGGGTCAAGGCATCGTGGACTAAACTCTTGCACATCACTTCAAAGATTTCAATTGTTCAAAAATATCCAGCTGGTTTACATGATTGCCTATTTCCTGTACGCATTTTAGAAAACGGCGAAGTTTTGATGAGTTGCAATGAACACGAGTTGGTGCTATACAATCCAAAAGAAAAGACGAACAAGACTGTTCTTGTTACTAATTTGTCCTGTGAAGGAATCATGTACGCAGAAACTTTGATTACACCGGTACTTGGTGGTGAAGTAGACGTCTGTAACCAAAAGTGTGGAGAAGCAGAAGCAAATAAAATACAGATCGACTACTTTGCAACGAGCTGTCTAAGCCTTGACACACCGGTTATGTCTGTTGGCAAGAAGCCTTGGAAGCTAGGGGTTAAGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

480

Amino Acids

54.46

Weight (kDa)

6.08

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 18 - 49 2.3e-07 F-box domain
F-box-like PF12937 18 - 51 2.8e-07 F-box-like
FBA_3 PF08268 115 - 415 9.8e-19 F-box associated beta propeller domain
FBA_1 PF07734 116 - 395 5.8e-17 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1311
AccB1I GGYRCC 1 cut(s) 599
AccI GTMKAC 6 cut(s) 234, 385, 396, 867, 975, 1305
AciI CCGC 1 cut(s) 894
AclWI GGATC 1 cut(s) 370
AcsI RAATTY 1 cut(s) 833
AcuI CTGAAG 2 cut(s) 272, 564
AcyI GRCGYC 1 cut(s) 1308
AfaI GTAC 6 cut(s) 442, 587, 952, 1140, 1265, 1290
AfiI CCNNNNNNNGG 4 cut(s) 443, 938, 945, 1292
AflIII ACRYGT 1 cut(s) 271
AgeI ACCGGT 2 cut(s) 1285, 1393
AgsI TTSAA 8 cut(s) 97, 199, 213, 839, 959, 1083, 1092, 1100
AjnI CCWGG 1 cut(s) 48
AloI GAACNNNNNNTCC 2 cut(s) 749, 781
Alw21I GWGCWC 3 cut(s) 490, 745, 800
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 1 cut(s) 370
ApeKI GCWGC 2 cut(s) 10, 312
ApoI RAATTY 1 cut(s) 833
ArsI GACNNNNNNTTYG 4 cut(s) 612, 644, 1029, 1061
AseI ATTAAT 1 cut(s) 996
AsiGI ACCGGT 2 cut(s) 1285, 1393
Asp700I GAANNNNTTC 1 cut(s) 101
AspS9I GGNCC 2 cut(s) 46, 883
AsuC2I CCSGG 1 cut(s) 25
AsuHPI GGTGA 3 cut(s) 691, 1039, 1310
AvaII GGWCC 2 cut(s) 46, 883
BaeI ACNNNNGTAYC 2 cut(s) 441, 474
BanI GGYRCC 1 cut(s) 599
BanII GRGCYC 3 cut(s) 490, 745, 800
BauI CACGAG 1 cut(s) 1184
Bbv12I GWGCWC 3 cut(s) 490, 745, 800
BbvI GCAGC 2 cut(s) 22, 299
BccI CCATC 4 cut(s) 260, 325, 748, 838
BceAI ACGGC 1 cut(s) 1171
BcgI CGANNNNNNTGC 4 cut(s) 90, 124, 1346, 1380
BciT130I CCWGG 1 cut(s) 50
BcnI CCSGG 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 4 cut(s) 563, 825, 990, 1427
BfmI CTRYAG 2 cut(s) 412, 772
BisI GCNGC 2 cut(s) 11, 313
BlsI GCNGC 2 cut(s) 12, 314
BmcAI AGTACT 1 cut(s) 952
Bme1390I CCNGG 2 cut(s) 25, 50
Bme18I GGWCC 2 cut(s) 46, 883
BmgT120I GGNCC 2 cut(s) 46, 883
BmiI GGNNCC 3 cut(s) 601, 907, 919
BmrFI CCNGG 2 cut(s) 25, 50
BmsI GCATC 2 cut(s) 37, 1061
BpmI CTGGAG 1 cut(s) 465
BpuEI CTTGAG 2 cut(s) 311, 784
BpuMI CCSGG 1 cut(s) 25
BsaHI GRCGYC 1 cut(s) 1308
BsaJI CCNNGG 1 cut(s) 1418
BsaWI WCCGGW 3 cut(s) 463, 1285, 1393
Bsc4I CCNNNNNNNGG 4 cut(s) 443, 938, 945, 1292
Bse118I RCCGGY 2 cut(s) 1285, 1393
Bse1I ACTGG 5 cut(s) 83, 106, 448, 858, 1002
Bse3DI GCAATG 2 cut(s) 697, 1183
BseBI CCWGG 1 cut(s) 50
BseDI CCNNGG 1 cut(s) 1418
BseGI GGATG 3 cut(s) 317, 505, 759
BseLI CCNNNNNNNGG 4 cut(s) 443, 938, 945, 1292
BseMI GCAATG 2 cut(s) 697, 1183
BseNI ACTGG 5 cut(s) 83, 106, 448, 858, 1002
BseXI GCAGC 2 cut(s) 22, 299
BshNI GGYRCC 1 cut(s) 599
BshTI ACCGGT 2 cut(s) 1285, 1393
BsiHKAI GWGCWC 3 cut(s) 490, 745, 800
BsiSI CCGG 5 cut(s) 25, 464, 939, 1286, 1394
BslFI GGGAC 1 cut(s) 581
BslI CCNNNNNNNGG 4 cut(s) 443, 938, 945, 1292
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 581
Bsp1286I GDGCHC 3 cut(s) 490, 745, 800
Bsp143I GATC 3 cut(s) 362, 924, 1353
BspACI CCGC 1 cut(s) 894
BspLI GGNNCC 3 cut(s) 601, 907, 919
BspMAI CTGCAG 1 cut(s) 416
BspPI GGATC 1 cut(s) 370
BspT107I GGYRCC 1 cut(s) 599
BsrDI GCAATG 2 cut(s) 697, 1183
BsrFI RCCGGY 2 cut(s) 1285, 1393
BsrI ACTGG 5 cut(s) 83, 106, 448, 858, 1002
BssAI RCCGGY 2 cut(s) 1285, 1393
BssECI CCNNGG 1 cut(s) 1418
BssMI GATC 3 cut(s) 362, 924, 1353
BssNAI GTATAC 1 cut(s) 976
BssNI GRCGYC 1 cut(s) 1308
BssSI CACGAG 1 cut(s) 1184
BssT1I CCWWGG 1 cut(s) 1418
Bst1107I GTATAC 1 cut(s) 976
Bst2BI CACGAG 1 cut(s) 1184
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 2 cut(s) 72, 1228
BstACI GRCGYC 1 cut(s) 1308
BstC8I GCNNGC 3 cut(s) 165, 280, 284
BstDEI CTNAG 3 cut(s) 910, 1379, 1440
BstF5I GGATG 3 cut(s) 317, 505, 759
BstKTI GATC 3 cut(s) 365, 927, 1356
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 3 cut(s) 362, 924, 1353
BstMWI GCNNNNNNNGC 4 cut(s) 173, 292, 1380, 1423
BstNI CCWGG 1 cut(s) 50
BstNSI RCATGY 2 cut(s) 167, 275
BstSCI CCNGG 2 cut(s) 23, 48
BstSFI CTRYAG 2 cut(s) 412, 772
BstV1I GCAGC 2 cut(s) 22, 299
BstX2I RGATCY 1 cut(s) 362
BstXI CCANNNNNNTGG 1 cut(s) 1325
BstYI RGATCY 1 cut(s) 362
BstZ17I GTATAC 1 cut(s) 976
BtsCI GGATG 3 cut(s) 317, 505, 759
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 4 cut(s) 113, 421, 540, 1009
Cac8I GCNNGC 3 cut(s) 165, 280, 284
Cfr10I RCCGGY 2 cut(s) 1285, 1393
Cfr13I GGNCC 2 cut(s) 46, 883
Csp6I GTAC 6 cut(s) 441, 586, 951, 1139, 1264, 1289
CspAI ACCGGT 2 cut(s) 1285, 1393
CviAII CATG 8 cut(s) 164, 257, 272, 389, 400, 651, 1121, 1261
CviQI GTAC 6 cut(s) 441, 586, 951, 1139, 1264, 1289
DdeI CTNAG 3 cut(s) 910, 1379, 1440
DpnI GATC 3 cut(s) 364, 926, 1355
DpnII GATC 3 cut(s) 362, 924, 1353
EciI GGCGGA 1 cut(s) 883
Ecl136II GAGCTC 3 cut(s) 488, 743, 798
Eco130I CCWWGG 1 cut(s) 1418
Eco24I GRGCYC 3 cut(s) 490, 745, 800
Eco32I GATATC 1 cut(s) 500
Eco47I GGWCC 2 cut(s) 46, 883
Eco53kI GAGCTC 3 cut(s) 488, 743, 798
Eco57I CTGAAG 2 cut(s) 272, 564
EcoICRI GAGCTC 3 cut(s) 488, 743, 798
EcoRII CCWGG 1 cut(s) 48
EcoRV GATATC 1 cut(s) 500
EcoT14I CCWWGG 1 cut(s) 1418
EcoT38I GRGCYC 3 cut(s) 490, 745, 800
ErhI CCWWGG 1 cut(s) 1418
FaeI CATG 8 cut(s) 167, 260, 275, 392, 403, 654, 1124, 1264
FaqI GGGAC 1 cut(s) 581
FatI CATG 8 cut(s) 163, 256, 271, 388, 399, 650, 1120, 1260
FblI GTMKAC 6 cut(s) 234, 385, 396, 867, 975, 1305
Fnu4HI GCNGC 2 cut(s) 11, 313
FokI GGATG 3 cut(s) 304, 492, 766
FriOI GRGCYC 3 cut(s) 490, 745, 800
Fsp4HI GCNGC 2 cut(s) 11, 313
FspBI CTAG 4 cut(s) 563, 825, 990, 1427
GluI GCNGC 2 cut(s) 11, 313
GsuI CTGGAG 1 cut(s) 465
HapII CCGG 5 cut(s) 25, 464, 939, 1286, 1394
Hin1I GRCGYC 1 cut(s) 1308
Hin1II CATG 8 cut(s) 167, 260, 275, 392, 403, 654, 1124, 1264
HincII GTYRAC 2 cut(s) 397, 868
HindII GTYRAC 2 cut(s) 397, 868
HindIII AAGCTT 2 cut(s) 193, 243
HpaII CCGG 5 cut(s) 25, 464, 939, 1286, 1394
HphI GGTGA 3 cut(s) 691, 1039, 1310
Hpy166II GTNNAC 9 cut(s) 235, 386, 397, 868, 976, 1017, 1059, 1118, 1306
Hpy188I TCNGA 4 cut(s) 142, 510, 917, 929
Hpy188III TCNNGA 3 cut(s) 35, 407, 839
Hpy8I GTNNAC 9 cut(s) 235, 386, 397, 868, 976, 1017, 1059, 1118, 1306
HpyAV CCTTC 2 cut(s) 590, 1247
HpyCH4III ACNGT 2 cut(s) 72, 1228
HpyCH4IV ACGT 4 cut(s) 354, 984, 1013, 1308
HpyCH4V TGCA 6 cut(s) 117, 278, 414, 1072, 1176, 1367
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 292, 1380, 1423
HpyF3I CTNAG 3 cut(s) 910, 1379, 1440
HpySE526I ACGT 4 cut(s) 354, 984, 1013, 1308
Hsp92I GRCGYC 1 cut(s) 1308
Hsp92II CATG 8 cut(s) 167, 260, 275, 392, 403, 654, 1124, 1264
Kzo9I GATC 3 cut(s) 362, 924, 1353
LmnI GCTCC 2 cut(s) 702, 740
Lsp1109I GCAGC 2 cut(s) 22, 299
LweI GCATC 2 cut(s) 37, 1061
MaeI CTAG 4 cut(s) 563, 825, 990, 1427
MaeII ACGT 4 cut(s) 354, 984, 1013, 1308
MaeIII GTNAC 5 cut(s) 416, 459, 849, 1234, 1313
MalI GATC 3 cut(s) 364, 926, 1355
MboI GATC 3 cut(s) 362, 924, 1353
MboII GAAGA 4 cut(s) 272, 557, 680, 959
MfeI CAATTG 1 cut(s) 1092
MflI RGATCY 1 cut(s) 362
MhlI GDGCHC 3 cut(s) 490, 745, 800
MluCI AATT 5 cut(s) 475, 833, 942, 1092, 1240
MlyI GAGTC 1 cut(s) 402
MmeI TCCRAC 1 cut(s) 895
MnlI CCTC 7 cut(s) 37, 69, 231, 325, 648, 669, 907
MroXI GAANNNNTTC 1 cut(s) 101
MseI TTAA 7 cut(s) 18, 87, 134, 606, 639, 996, 1434
MslI CAYNNNNRTG 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 1112
MspI CCGG 5 cut(s) 25, 464, 939, 1286, 1394
MspR9I CCNGG 2 cut(s) 25, 50
MunI CAATTG 1 cut(s) 1092
MvaI CCWGG 1 cut(s) 50
MwoI GCNNNNNNNGC 4 cut(s) 173, 292, 1380, 1423
NciI CCSGG 1 cut(s) 25
NdeII GATC 3 cut(s) 362, 924, 1353
NlaIII CATG 8 cut(s) 167, 260, 275, 392, 403, 654, 1124, 1264
NlaIV GGNNCC 3 cut(s) 601, 907, 919
NmuCI GTSAC 2 cut(s) 416, 849
NspI RCATGY 2 cut(s) 167, 275
PaeI GCATGC 1 cut(s) 167
PciI ACATGT 1 cut(s) 271
PdmI GAANNNNTTC 1 cut(s) 101
PfeI GAWTC 9 cut(s) 102, 143, 403, 433, 522, 533, 610, 682, 1257
PfoI TCCNGGA 1 cut(s) 48
PinAI ACCGGT 2 cut(s) 1285, 1393
PkrI GCNGC 2 cut(s) 12, 314
PleI GAGTC 1 cut(s) 401
PpsI GAGTC 1 cut(s) 401
PscI ACATGT 1 cut(s) 271
PshBI ATTAAT 1 cut(s) 996
Psp124BI GAGCTC 3 cut(s) 490, 745, 800
Psp6I CCWGG 1 cut(s) 48
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 3 cut(s) 601, 907, 919
PspPI GGNCC 2 cut(s) 46, 883
PstI CTGCAG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 1112
RsaI GTAC 6 cut(s) 442, 587, 952, 1140, 1265, 1290
RsaNI GTAC 6 cut(s) 441, 586, 951, 1139, 1264, 1289
RseI CAYNNNNRTG 1 cut(s) 261
SacI GAGCTC 3 cut(s) 490, 745, 800
SalI GTCGAC 2 cut(s) 395, 866
SaqAI TTAA 7 cut(s) 18, 87, 134, 606, 639, 996, 1434
SatI GCNGC 2 cut(s) 11, 313
Sau3AI GATC 3 cut(s) 362, 924, 1353
Sau96I GGNCC 2 cut(s) 46, 883
ScaI AGTACT 1 cut(s) 952
SchI GAGTC 1 cut(s) 402
ScrFI CCNGG 2 cut(s) 25, 50
SduI GDGCHC 3 cut(s) 490, 745, 800
SfaNI GCATC 2 cut(s) 37, 1061
SfcI CTRYAG 2 cut(s) 412, 772
SinI GGWCC 2 cut(s) 46, 883
SmiMI CAYNNNNRTG 1 cut(s) 261
SmlI CTYRAG 2 cut(s) 290, 799
SmoI CTYRAG 2 cut(s) 290, 799
SphI GCATGC 1 cut(s) 167
Sse9I AATT 5 cut(s) 475, 833, 942, 1092, 1240
SsiI CCGC 1 cut(s) 894
SspMI CTAG 4 cut(s) 563, 825, 990, 1427
SstI GAGCTC 3 cut(s) 490, 745, 800
StyD4I CCNGG 2 cut(s) 23, 48
StyI CCWWGG 1 cut(s) 1418
TaaI ACNGT 2 cut(s) 72, 1228
TaiI ACGT 4 cut(s) 357, 987, 1016, 1311
TaqI TCGA 9 cut(s) 74, 216, 396, 406, 484, 768, 794, 867, 1356
TasI AATT 5 cut(s) 475, 833, 942, 1092, 1240
TatI WGTACW 2 cut(s) 440, 950
TfiI GAWTC 9 cut(s) 102, 143, 403, 433, 522, 533, 610, 682, 1257
Tru1I TTAA 7 cut(s) 18, 87, 134, 606, 639, 996, 1434
Tru9I TTAA 7 cut(s) 18, 87, 134, 606, 639, 996, 1434
TscAI CASTG 4 cut(s) 113, 421, 547, 1009
TseFI GTSAC 2 cut(s) 416, 849
TseI GCWGC 2 cut(s) 10, 312
Tsp45I GTSAC 2 cut(s) 416, 849
TspDTI ATGAA 9 cut(s) 173, 273, 416, 609, 639, 681, 708, 1046, 1194
TspGWI ACGGA 4 cut(s) 220, 715, 753, 918
TspRI CASTG 4 cut(s) 113, 421, 547, 1009
VpaK11BI GGWCC 2 cut(s) 46, 883
VspI ATTAAT 1 cut(s) 996
XapI RAATTY 1 cut(s) 833
XceI RCATGY 2 cut(s) 167, 275
XmiI GTMKAC 6 cut(s) 234, 385, 396, 867, 975, 1305
XmnI GAANNNNTTC 1 cut(s) 101
XspI CTAG 4 cut(s) 563, 825, 990, 1427
ZraI GACGTC 1 cut(s) 1309
ZrmI AGTACT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.