Rroxscaffold_2G00103640

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013699
Physical Location & Seq
Reverse (-)
26630620 .. 26633880
3261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00103640.1

Sequence Viewer

Length: 1305 bp
ATGGCGCGTGGTATGAAACAGTGGGTTGGACTTGCAACTGAAAATAACATAAAAGAGCTAGGTCTCCATGTTCACACAAGAGGAAATAATGTTCACAATTTTCCCAGGAATGCGTTTGCTTCAGATACACTAACTGGATTAAGGCTGCAGAGCTGTAAACTGGAAACTAGCTGCGCTGTAAAGCTTCCCCATCTGCAAAAGCTATATTTGCGGGATTGTAATGTTGATGAACAGAGAATCCAGCATATTATTTCCAGCTGCCCTTTAATTGAGGATTTGAGACTCATACGTTGCACAGGGTTGAAGTTTCTGCAGATTTCAAGTCTTCTTAAGCTTGATAGGGTTGAAGTATACCACTGCCGCGGACTGAAGAAGATTGAAGTTAAAGCTGCAAAGCTTCAGACATTTTGGTATTGTGGGAAGAATCGCACGTCTTGCAAACTTATCTTGAGTGGTTGTGAGTCTCTGAAAAGATTGACACTAGAGGACAAAAAAATGACAGACAATCAGTTTCGTGAACTATTCTTTGGCCTTCCTCTCCTCGAGAAGCTGGACCTAAGTAAATGCCTCAAATTGAAAAATATTACAATTGCAAGTCATCGACTAAACAGATTAGTCTTGAGGGGTTGTGAGAATCTACAGGGAGCAGAGATTGATACTCCAAATCTTGTTTCATTCGAGTATCAAGGTGATAAAATGCCCTTTTCTTTTCTGAATCCTGTATCTTTGAAAGAGGCCAAGCTTTCTTTTGGGTCGGCTTCTGCTGGTCAGCTCAAATTCGATCACATTAAAGACGAGCCTTTTTGGTTTAAAAAGTTGCGGGATTTTCTTGGCAAGTTTGATCACAATACAGGCTTCAAACTGGTTGTCCGCACTAACAAGAATGTTATCATCTACGAAGATCTAAGAGGAATTTCACTTCCTCCTGTACGTGGTCTCAAACTTGAAATAATCCAGTCTTCCGTGGGTACTGAAGAACTATTAAACAGTTCATTGCAGAAATGGCACCCAGAGACCCTATCTGTAGTATCATCTTCTAGCAGTGAATTCCCCAAGTTGGTACATGAGAAGATCATCGAGAGTAAGAAGGATCCAACCTGCTGCACATCTAACCATTCAAACAATAAATGTTGGCGGCATTCTTTGAAGTTTGGCAAGAAAGTGAACTTGAAGCGGGCCAAAGGCAAGTCGGGCTGGATTGCTTGGTTGAAGTTGAAGTCTTGTCCAACTGACCTGTACGAGATTACTTGTTTTAGTTTATTAAATAAAAAATCTGATAAGCATGGTAGGAAGGCATCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBD Jacalin

Protein Analysis

434

Amino Acids

49.5

Weight (kDa)

9.58

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 5 - 144 3.7e-20 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 6 - 138 8.4e-16 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 151 - 249 5.9e-12 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013699)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g32840 FvH4_6g32840 FvH4_6g32840 FvH4_6g32840
malus_domestica MD09G1197600.v1.1 MD17G1178500.v1.1
prunus_persica Prupe.3G053600_v2.0.a1
pyrus_communis pycom09g11420 pycom17g18740
rosa_chinensis RchiOBHm_Chr2g0142131
rosa_laevigata RLG00000019948
rosa_roxburghii Rroxscaffold_2G00103640
rosa_rugosa Rorug02G0370700
rosa_samantha Rh2AG422600 Rh2BG432800 Rh2CG409500 Rh2DG442500
rosa_wichuraiana Rw2G034630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1106
AccB1I GGYRCC 1 cut(s) 1005
AccI GTMKAC 1 cut(s) 351
AccII CGCG 2 cut(s) 7, 363
AciI CCGC 7 cut(s) 211, 361, 363, 820, 871, 1135, 1174
AclWI GGATC 2 cut(s) 1085, 1098
AcsI RAATTY 3 cut(s) 776, 912, 1046
AcuI CTGAAG 4 cut(s) 105, 383, 389, 993
AfaI GTAC 4 cut(s) 930, 970, 1062, 1238
AfiI CCNNNNNNNGG 2 cut(s) 932, 1057
AflII CTTAAG 1 cut(s) 329
AjiI CACGTC 1 cut(s) 432
AjnI CCWGG 1 cut(s) 104
AjuI GAANNNNNNNTTGG 4 cut(s) 510, 542, 655, 687
AloI GAACNNNNNNTCC 4 cut(s) 75, 107, 222, 254
Alw26I GTCTC 5 cut(s) 68, 274, 468, 941, 1007
AlwI GGATC 2 cut(s) 1085, 1098
Ama87I CYCGRG 1 cut(s) 542
AoxI GGCC 3 cut(s) 529, 735, 1176
ApeKI GCWGC 5 cut(s) 145, 171, 258, 389, 1101
ApoI RAATTY 3 cut(s) 776, 912, 1046
ArsI GACNNNNNNTTYG 2 cut(s) 852, 884
AspLEI GCGC 2 cut(s) 7, 176
AspS9I GGNCC 2 cut(s) 553, 1176
AsuHPI GGTGA 1 cut(s) 701
AvaI CYCGRG 1 cut(s) 542
AvaII GGWCC 1 cut(s) 553
BamHI GGATCC 1 cut(s) 1090
BanI GGYRCC 1 cut(s) 1005
BbsI GAAGAC 2 cut(s) 317, 951
BbvI GCAGC 5 cut(s) 132, 158, 245, 376, 1088
BccI CCATC 1 cut(s) 198
BciT130I CCWGG 1 cut(s) 106
BclI TGATCA 1 cut(s) 841
BcoDI GTCTC 5 cut(s) 68, 274, 468, 941, 1007
BfaI CTAG 4 cut(s) 59, 168, 482, 1038
BfmI CTRYAG 4 cut(s) 146, 311, 638, 1023
BfrI CTTAAG 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 1106
BglII AGATCT 1 cut(s) 901
BisI GCNGC 7 cut(s) 146, 172, 259, 361, 390, 1102, 1136
BlsI GCNGC 7 cut(s) 147, 173, 260, 362, 391, 1103, 1137
Bme1390I CCNGG 1 cut(s) 106
Bme18I GGWCC 1 cut(s) 553
BmeT110I CYCGRG 1 cut(s) 542
BmgBI CACGTC 1 cut(s) 432
BmgT120I GGNCC 2 cut(s) 553, 1176
BmiI GGNNCC 2 cut(s) 1007, 1092
BmrFI CCNGG 1 cut(s) 106
BpiI GAAGAC 2 cut(s) 317, 951
BpuEI CTTGAG 2 cut(s) 469, 640
BsaAI YACGTR 1 cut(s) 932
BsaI GGTCTC 3 cut(s) 68, 941, 1007
BsaJI CCNNGG 3 cut(s) 104, 361, 963
Bsc4I CCNNNNNNNGG 2 cut(s) 932, 1057
Bse1I ACTGG 4 cut(s) 139, 165, 867, 955
Bse3DI GCAATG 1 cut(s) 992
BseBI CCWGG 1 cut(s) 106
BseDI CCNNGG 3 cut(s) 104, 361, 963
BseLI CCNNNNNNNGG 2 cut(s) 932, 1057
BseMI GCAATG 1 cut(s) 992
BseNI ACTGG 4 cut(s) 139, 165, 867, 955
BseRI GAGGAG 1 cut(s) 530
BseXI GCAGC 5 cut(s) 132, 158, 245, 376, 1088
BsgI GTGCAG 1 cut(s) 1087
Bsh1236I CGCG 2 cut(s) 7, 363
BshFI GGCC 3 cut(s) 531, 737, 1178
BshNI GGYRCC 1 cut(s) 1005
BsiHKCI CYCGRG 1 cut(s) 542
BslI CCNNNNNNNGG 2 cut(s) 932, 1057
BsmAI GTCTC 5 cut(s) 68, 274, 468, 941, 1007
BsmI GAATGC 2 cut(s) 115, 1138
BsnI GGCC 3 cut(s) 531, 737, 1178
Bso31I GGTCTC 3 cut(s) 68, 941, 1007
BsoBI CYCGRG 1 cut(s) 542
Bsp143I GATC 5 cut(s) 781, 841, 901, 1071, 1090
BspACI CCGC 7 cut(s) 211, 361, 363, 820, 871, 1135, 1174
BspANI GGCC 3 cut(s) 531, 737, 1178
BspFNI CGCG 2 cut(s) 7, 363
BspLI GGNNCC 2 cut(s) 1007, 1092
BspMAI CTGCAG 2 cut(s) 150, 315
BspMI ACCTGC 1 cut(s) 1106
BspPI GGATC 2 cut(s) 1085, 1098
BspT107I GGYRCC 1 cut(s) 1005
BspTI CTTAAG 1 cut(s) 329
BspTNI GGTCTC 3 cut(s) 68, 941, 1007
BsrDI GCAATG 1 cut(s) 992
BsrI ACTGG 4 cut(s) 139, 165, 867, 955
BssECI CCNNGG 3 cut(s) 104, 361, 963
BssMI GATC 5 cut(s) 781, 841, 901, 1071, 1090
BssNAI GTATAC 1 cut(s) 352
Bst1107I GTATAC 1 cut(s) 352
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 21, 989
BstAFI CTTAAG 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 435
BstBAI YACGTR 1 cut(s) 932
BstC8I GCNNGC 1 cut(s) 1176
BstDEI CTNAG 2 cut(s) 557, 905
BstDSI CCRYGG 2 cut(s) 361, 963
BstFNI CGCG 2 cut(s) 7, 363
BstHHI GCGC 2 cut(s) 7, 176
BstKTI GATC 5 cut(s) 784, 844, 904, 1074, 1093
BstMAI GTCTC 5 cut(s) 68, 274, 468, 941, 1007
BstMBI GATC 5 cut(s) 781, 841, 901, 1071, 1090
BstMWI GCNNNNNNNGC 4 cut(s) 208, 435, 1003, 1191
BstNI CCWGG 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 104
BstSFI CTRYAG 4 cut(s) 146, 311, 638, 1023
BstUI CGCG 2 cut(s) 7, 363
BstV1I GCAGC 5 cut(s) 132, 158, 245, 376, 1088
BstV2I GAAGAC 2 cut(s) 317, 951
BstX2I RGATCY 2 cut(s) 901, 1090
BstYI RGATCY 2 cut(s) 901, 1090
BstZ17I GTATAC 1 cut(s) 352
BsuRI GGCC 3 cut(s) 531, 737, 1178
BtgI CCRYGG 2 cut(s) 361, 963
BtrI CACGTC 1 cut(s) 432
BtsI GCAGTG 2 cut(s) 355, 1048
BtsIMutI CAGTG 3 cut(s) 26, 355, 1048
BveI ACCTGC 1 cut(s) 1106
Cac8I GCNNGC 1 cut(s) 1176
CfoI GCGC 2 cut(s) 7, 176
Cfr13I GGNCC 2 cut(s) 553, 1176
Cfr42I CCGCGG 1 cut(s) 364
Csp6I GTAC 4 cut(s) 929, 969, 1061, 1237
CviAII CATG 3 cut(s) 68, 1064, 1283
CviQI GTAC 4 cut(s) 929, 969, 1061, 1237
DdeI CTNAG 2 cut(s) 557, 905
DpnI GATC 5 cut(s) 783, 843, 903, 1073, 1092
DpnII GATC 5 cut(s) 781, 841, 901, 1071, 1090
DraI TTTAAA 1 cut(s) 811
Eco31I GGTCTC 3 cut(s) 68, 941, 1007
Eco47I GGWCC 1 cut(s) 553
Eco57I CTGAAG 4 cut(s) 105, 383, 389, 993
Eco88I CYCGRG 1 cut(s) 542
EcoRI GAATTC 1 cut(s) 1046
EcoRII CCWGG 1 cut(s) 104
FaeI CATG 3 cut(s) 71, 1067, 1286
FaiI YATR 9 cut(s) 14, 50, 69, 205, 246, 287, 352, 1065, 1284
FatI CATG 3 cut(s) 67, 1063, 1282
FauI CCCGC 3 cut(s) 204, 813, 1167
FbaI TGATCA 1 cut(s) 841
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 7 cut(s) 146, 172, 259, 361, 390, 1102, 1136
Fsp4HI GCNGC 7 cut(s) 146, 172, 259, 361, 390, 1102, 1136
FspBI CTAG 4 cut(s) 59, 168, 482, 1038
GlaI GCGC 2 cut(s) 6, 175
GluI GCNGC 7 cut(s) 146, 172, 259, 361, 390, 1102, 1136
HaeIII GGCC 3 cut(s) 531, 737, 1178
HhaI GCGC 2 cut(s) 7, 176
Hin1II CATG 3 cut(s) 71, 1067, 1286
Hin6I GCGC 2 cut(s) 5, 174
HinP1I GCGC 2 cut(s) 5, 174
HindIII AAGCTT 4 cut(s) 182, 332, 395, 740
HinfI GANTC 6 cut(s) 237, 282, 424, 461, 634, 715
HphI GGTGA 1 cut(s) 701
Hpy166II GTNNAC 6 cut(s) 73, 94, 158, 352, 518, 1165
Hpy188I TCNGA 5 cut(s) 124, 402, 468, 714, 1276
Hpy188III TCNNGA 5 cut(s) 448, 515, 544, 619, 1078
Hpy8I GTNNAC 6 cut(s) 73, 94, 158, 352, 518, 1165
HpyAV CCTTC 3 cut(s) 542, 1081, 1285
HpyCH4III ACNGT 2 cut(s) 21, 989
HpyCH4IV ACGT 3 cut(s) 289, 431, 931
HpyF10VI GCNNNNNNNGC 4 cut(s) 208, 435, 1003, 1191
HpyF3I CTNAG 2 cut(s) 557, 905
HpySE526I ACGT 3 cut(s) 289, 431, 931
Hsp92II CATG 3 cut(s) 71, 1067, 1286
HspAI GCGC 2 cut(s) 5, 174
Ksp22I TGATCA 1 cut(s) 841
KspI CCGCGG 1 cut(s) 364
Kzo9I GATC 5 cut(s) 781, 841, 901, 1071, 1090
LmnI GCTCC 1 cut(s) 644
Lsp1109I GCAGC 5 cut(s) 132, 158, 245, 376, 1088
MaeI CTAG 4 cut(s) 59, 168, 482, 1038
MaeII ACGT 3 cut(s) 289, 431, 931
MalI GATC 5 cut(s) 783, 843, 903, 1073, 1092
MboI GATC 5 cut(s) 781, 841, 901, 1071, 1090
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 2 cut(s) 901, 1090
MluCI AATT 7 cut(s) 97, 267, 572, 588, 776, 912, 1046
MlyI GAGTC 2 cut(s) 276, 470
MmeI TCCRAC 3 cut(s) 7, 1118, 1250
MseI TTAA 9 cut(s) 140, 266, 330, 384, 789, 810, 983, 1262, 1303
MspA1I CMGCKG 2 cut(s) 258, 363
MspCI CTTAAG 1 cut(s) 329
MspR9I CCNGG 1 cut(s) 106
MunI CAATTG 1 cut(s) 588
Mva1269I GAATGC 2 cut(s) 115, 1138
MvaI CCWGG 1 cut(s) 106
MvnI CGCG 2 cut(s) 7, 363
MwoI GCNNNNNNNGC 4 cut(s) 208, 435, 1003, 1191
NdeII GATC 5 cut(s) 781, 841, 901, 1071, 1090
NlaIII CATG 3 cut(s) 71, 1067, 1286
NlaIV GGNNCC 2 cut(s) 1007, 1092
PaeR7I CTCGAG 1 cut(s) 542
PctI GAATGC 2 cut(s) 115, 1138
PfeI GAWTC 4 cut(s) 237, 424, 634, 715
PkrI GCNGC 7 cut(s) 147, 173, 260, 362, 391, 1103, 1137
PleI GAGTC 2 cut(s) 276, 469
PpsI GAGTC 2 cut(s) 276, 469
Ppu21I YACGTR 1 cut(s) 932
Psp6I CCWGG 1 cut(s) 104
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 2 cut(s) 1007, 1092
PspPI GGNCC 2 cut(s) 553, 1176
PstI CTGCAG 2 cut(s) 150, 315
PsuI RGATCY 2 cut(s) 901, 1090
PvuII CAGCTG 1 cut(s) 258
RsaI GTAC 4 cut(s) 930, 970, 1062, 1238
RsaNI GTAC 4 cut(s) 929, 969, 1061, 1237
SacII CCGCGG 1 cut(s) 364
SaqAI TTAA 9 cut(s) 140, 266, 330, 384, 789, 810, 983, 1262, 1303
SatI GCNGC 7 cut(s) 146, 172, 259, 361, 390, 1102, 1136
Sau3AI GATC 5 cut(s) 781, 841, 901, 1071, 1090
Sau96I GGNCC 2 cut(s) 553, 1176
SchI GAGTC 2 cut(s) 276, 470
ScrFI CCNGG 1 cut(s) 106
SfcI CTRYAG 4 cut(s) 146, 311, 638, 1023
Sfr274I CTCGAG 1 cut(s) 542
Sfr303I CCGCGG 1 cut(s) 364
SgrBI CCGCGG 1 cut(s) 364
SinI GGWCC 1 cut(s) 553
SlaI CTCGAG 1 cut(s) 542
SmlI CTYRAG 4 cut(s) 329, 448, 542, 619
SmoI CTYRAG 4 cut(s) 329, 448, 542, 619
Sse9I AATT 7 cut(s) 97, 267, 572, 588, 776, 912, 1046
SsiI CCGC 7 cut(s) 211, 361, 363, 820, 871, 1135, 1174
SspI AATATT 1 cut(s) 583
SspMI CTAG 4 cut(s) 59, 168, 482, 1038
StyD4I CCNGG 1 cut(s) 104
TaaI ACNGT 2 cut(s) 21, 989
TaiI ACGT 3 cut(s) 292, 434, 934
TaqI TCGA 5 cut(s) 543, 601, 678, 780, 1077
TasI AATT 7 cut(s) 97, 267, 572, 588, 776, 912, 1046
TauI GCSGC 2 cut(s) 363, 1138
TfiI GAWTC 4 cut(s) 237, 424, 634, 715
Tru1I TTAA 9 cut(s) 140, 266, 330, 384, 789, 810, 983, 1262, 1303
Tru9I TTAA 9 cut(s) 140, 266, 330, 384, 789, 810, 983, 1262, 1303
TscAI CASTG 3 cut(s) 26, 362, 1048
TseI GCWGC 5 cut(s) 145, 171, 258, 389, 1101
TspDTI ATGAA 4 cut(s) 29, 243, 663, 981
TspGWI ACGGA 1 cut(s) 952
TspRI CASTG 3 cut(s) 26, 362, 1048
Vha464I CTTAAG 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 3 cut(s) 776, 912, 1046
XhoI CTCGAG 1 cut(s) 542
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 4 cut(s) 59, 168, 482, 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.