Rw2G034630

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013699
Physical Location & Seq
Forward (+)
56973910 .. 56977142
3233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G034630.1

Sequence Viewer

Length: 1389 bp
ATGAAGAGGAAAGGCATGTCTAGCACACCACAGGATGAAACCGGTGCTGTTGATTTGATTACACAAGCACCTGGAATTGAAAGAAAGAGAAAGAGAAAGGAATCTAATACCATGGACCAGAACGTTGAGCGACAAAAGCAGCAAACTGCCGAAAATTCAGAGCGCTTGGACAAATGCATTACACAAGAAGCTGGATCTCGAAAAAAACGAAAGAGAGAAAAATCTAATATTACTGGCAAGAGGGGGAAGCTTAGAAAGAAGCAACCTGGTGGAGTTGTAAATGACATTGAAAAAAGTGTTGAGTCCACTGATCATATCTCACAATTGCCTGAATCTGTTATTCATCACATCCTTTCTCTCATTCGTTGTACAAAAGGTGCTGCTCGGACCAGTGTGTTGTCCAAGAGGTGGAGGGATGTATGGACTTCTTTCTCTGTTTTGACATTTGATCAACGCAAGTTTCAGAAACCAGAGGGAGTTCAAGATAAGAGCAAGAATGAGATGTTTAAGGACTTTGTTGATCGGTCTCTTCAGAGCCAACTTGAGCAAAATTTAGGTATACGTAAATTTGAGCTACATTTGACCTCCTATGATCAAGAAATGGCACGTGGTATGGAACAGTGGGTTGGACTTGCAACTGAAAATAACATAAAAGAGCTAGGTCTCCATGTTCACACAAGAGGAAATAATGTTCACAATTTTCCCAGGAATGCGTTTGCTTCAGATACACTAACTCGATTAAGGCTGCAGAGCTGTAAACTGGAAACTAGCTGCGCTGTAAAGCTTCCCCATCTGCAAAAGCTATATTTGCGGGATTGTAATGTTGATGAACAGAGAATCCAGCATATTATTTCCAGCTGCCCTTTAATTGAGGATTTGAGACTCATACGTTGCACGGGGTTGAAGTTTCTGCAGATTTCAAGTCTCCTTAAGCTTGATAGGGTTGAAGTATACCACTGCCGCGGACTGAAGAAGATTGAAGTTAAAGCTGCAAAGCTTCAGACATTTTGGTATTGTGGGAAGAATCGCACGTCTTGCAAACTTATCTTGAGTGGTTGTGAGTCTCTGAAAAGATTGACACTAGAGGACAAAAAAATGACAGACAATCAGTTTCGTGAACTATTCTTTGGCCTTCCTCTCCTCGAGAAGCTGGACCTAAGTAAATGCCTCAAATTGAAAAATATTACAATTGCAAGTCATCGACTAAACAGATTAGTCTTGAGGGGTTGCGAGAATCTACAGGGAGCCGAGATTGATACTCCAAATCTTCTTTCATTAGAGTATCAAGGTGATAAAATGCCCTTTTCTTTTCTGAATCCTATCTTTGAAGGAGGCCAAGCTTTCTTTTGGGTCGGCTTCTGCTGGTCAGCTCAAATTCGATCACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBD Jacalin

Protein Analysis

462

Amino Acids

53.24

Weight (kDa)

9.59

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 184 - 344 1.4e-23 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 206 - 338 8.3e-17 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 351 - 437 5.5e-10 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013699)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g32840 FvH4_6g32840 FvH4_6g32840 FvH4_6g32840
malus_domestica MD09G1197600.v1.1 MD17G1178500.v1.1
prunus_persica Prupe.3G053600_v2.0.a1
pyrus_communis pycom09g11420 pycom17g18740
rosa_chinensis RchiOBHm_Chr2g0142131
rosa_laevigata RLG00000019948
rosa_roxburghii Rroxscaffold_2G00103640
rosa_rugosa Rorug02G0370700
rosa_samantha Rh2AG422600 Rh2BG432800 Rh2CG409500 Rh2DG442500
rosa_wichuraiana Rw2G034630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 408
AccI GTMKAC 2 cut(s) 559, 951
AccII CGCG 1 cut(s) 963
AciI CCGC 3 cut(s) 811, 961, 963
AclI AACGTT 1 cut(s) 123
AclWI GGATC 1 cut(s) 202
AcsI RAATTY 4 cut(s) 154, 550, 566, 1374
AcuI CTGAAG 4 cut(s) 515, 705, 983, 989
AcvI CACGTG 1 cut(s) 608
AfaI GTAC 1 cut(s) 370
AfeI AGCGCT 1 cut(s) 164
AfiI CCNNNNNNNGG 1 cut(s) 408
AflII CTTAAG 1 cut(s) 929
AgeI ACCGGT 1 cut(s) 41
AgsI TTSAA 9 cut(s) 80, 290, 482, 904, 921, 947, 980, 1177, 1328
AjiI CACGTC 1 cut(s) 1032
AjnI CCWGG 3 cut(s) 70, 265, 704
AjuI GAANNNNNNNTTGG 6 cut(s) 609, 641, 1110, 1142, 1255, 1287
AloI GAACNNNNNNTCC 4 cut(s) 675, 707, 822, 854
Alw26I GTCTC 5 cut(s) 531, 668, 874, 929, 1068
AlwI GGATC 1 cut(s) 202
Ama87I CYCGRG 1 cut(s) 1142
Aor51HI AGCGCT 1 cut(s) 164
AoxI GGCC 2 cut(s) 1129, 1333
ApeKI GCWGC 6 cut(s) 139, 380, 745, 771, 858, 989
ApoI RAATTY 4 cut(s) 154, 550, 566, 1374
AsiGI ACCGGT 1 cut(s) 41
AspLEI GCGC 2 cut(s) 165, 776
AspS9I GGNCC 3 cut(s) 115, 387, 1153
AsuHPI GGTGA 1 cut(s) 1301
AvaI CYCGRG 1 cut(s) 1142
AvaII GGWCC 3 cut(s) 115, 387, 1153
BbrPI CACGTG 1 cut(s) 608
BbvI GCAGC 6 cut(s) 151, 367, 732, 758, 845, 976
BccI CCATC 1 cut(s) 798
BciT130I CCWGG 3 cut(s) 72, 267, 706
BclI TGATCA 3 cut(s) 310, 448, 592
BcoDI GTCTC 5 cut(s) 531, 668, 874, 929, 1068
BfaI CTAG 4 cut(s) 21, 659, 768, 1082
BfmI CTRYAG 3 cut(s) 746, 911, 1238
BfoI RGCGCY 1 cut(s) 166
BfrI CTTAAG 1 cut(s) 929
BisI GCNGC 7 cut(s) 140, 381, 746, 772, 859, 961, 990
BlsI GCNGC 7 cut(s) 141, 382, 747, 773, 860, 962, 991
Bme1390I CCNGG 3 cut(s) 72, 267, 706
Bme18I GGWCC 3 cut(s) 115, 387, 1153
BmeT110I CYCGRG 1 cut(s) 1142
BmgBI CACGTC 1 cut(s) 1032
BmgT120I GGNCC 3 cut(s) 115, 387, 1153
BmiI GGNNCC 1 cut(s) 1246
BmrFI CCNGG 3 cut(s) 72, 267, 706
BpuEI CTTGAG 3 cut(s) 563, 1069, 1240
BsaAI YACGTR 2 cut(s) 563, 608
BsaI GGTCTC 2 cut(s) 531, 668
BsaJI CCNNGG 3 cut(s) 111, 704, 961
BsaWI WCCGGW 1 cut(s) 41
Bsc4I CCNNNNNNNGG 1 cut(s) 408
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 3 cut(s) 238, 390, 765
BseBI CCWGG 3 cut(s) 72, 267, 706
BseDI CCNNGG 3 cut(s) 111, 704, 961
BseGI GGATG 3 cut(s) 40, 348, 421
BseLI CCNNNNNNNGG 1 cut(s) 408
BseNI ACTGG 3 cut(s) 238, 390, 765
BseRI GAGGAG 1 cut(s) 1130
BseXI GCAGC 6 cut(s) 151, 367, 732, 758, 845, 976
Bsh1236I CGCG 1 cut(s) 963
BshFI GGCC 2 cut(s) 1131, 1335
BshTI ACCGGT 1 cut(s) 41
BsiHKCI CYCGRG 1 cut(s) 1142
BsiSI CCGG 1 cut(s) 42
BslI CCNNNNNNNGG 1 cut(s) 408
BsmAI GTCTC 5 cut(s) 531, 668, 874, 929, 1068
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 2 cut(s) 1131, 1335
Bso31I GGTCTC 2 cut(s) 531, 668
BsoBI CYCGRG 1 cut(s) 1142
Bsp1407I TGTACA 1 cut(s) 368
Bsp143I GATC 6 cut(s) 194, 310, 448, 520, 592, 1379
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 3 cut(s) 811, 961, 963
BspANI GGCC 2 cut(s) 1131, 1335
BspFNI CGCG 1 cut(s) 963
BspLI GGNNCC 1 cut(s) 1246
BspMAI CTGCAG 2 cut(s) 750, 915
BspPI GGATC 1 cut(s) 202
BspTI CTTAAG 1 cut(s) 929
BspTNI GGTCTC 2 cut(s) 531, 668
BsrFI RCCGGY 1 cut(s) 41
BsrGI TGTACA 1 cut(s) 368
BsrI ACTGG 3 cut(s) 238, 390, 765
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 3 cut(s) 111, 704, 961
BssMI GATC 6 cut(s) 194, 310, 448, 520, 592, 1379
BssNAI GTATAC 2 cut(s) 560, 952
BssT1I CCWWGG 1 cut(s) 111
Bst1107I GTATAC 2 cut(s) 560, 952
Bst2UI CCWGG 3 cut(s) 72, 267, 706
Bst4CI ACNGT 1 cut(s) 621
Bst6I CTCTTC 1 cut(s) 534
BstAFI CTTAAG 1 cut(s) 929
BstAPI GCANNNNNTGC 1 cut(s) 1035
BstAUI TGTACA 1 cut(s) 368
BstBAI YACGTR 2 cut(s) 563, 608
BstDEI CTNAG 2 cut(s) 251, 1157
BstDSI CCRYGG 2 cut(s) 111, 961
BstF5I GGATG 3 cut(s) 40, 348, 421
BstFNI CGCG 1 cut(s) 963
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 2 cut(s) 165, 776
BstKTI GATC 6 cut(s) 197, 313, 451, 523, 595, 1382
BstMAI GTCTC 5 cut(s) 531, 668, 874, 929, 1068
BstMBI GATC 6 cut(s) 194, 310, 448, 520, 592, 1379
BstMWI GCNNNNNNNGC 4 cut(s) 21, 136, 808, 1035
BstNI CCWGG 3 cut(s) 72, 267, 706
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 3 cut(s) 70, 265, 704
BstSFI CTRYAG 3 cut(s) 746, 911, 1238
BstSNI TACGTA 1 cut(s) 563
BstUI CGCG 1 cut(s) 963
BstV1I GCAGC 6 cut(s) 151, 367, 732, 758, 845, 976
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
BstZ17I GTATAC 2 cut(s) 560, 952
BsuRI GGCC 2 cut(s) 1131, 1335
BtgI CCRYGG 2 cut(s) 111, 961
BtrI CACGTC 1 cut(s) 1032
BtsCI GGATG 3 cut(s) 40, 348, 421
BtsI GCAGTG 1 cut(s) 955
BtsIMutI CAGTG 4 cut(s) 306, 397, 626, 955
CfoI GCGC 2 cut(s) 165, 776
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 3 cut(s) 115, 387, 1153
Cfr42I CCGCGG 1 cut(s) 964
CsiI ACCWGGT 1 cut(s) 265
Csp6I GTAC 1 cut(s) 369
CspAI ACCGGT 1 cut(s) 41
CviAII CATG 3 cut(s) 16, 112, 668
CviQI GTAC 1 cut(s) 369
DdeI CTNAG 2 cut(s) 251, 1157
DpnI GATC 6 cut(s) 196, 312, 450, 522, 594, 1381
DpnII GATC 6 cut(s) 194, 310, 448, 520, 592, 1379
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
Eco105I TACGTA 1 cut(s) 563
Eco130I CCWWGG 1 cut(s) 111
Eco31I GGTCTC 2 cut(s) 531, 668
Eco47I GGWCC 3 cut(s) 115, 387, 1153
Eco47III AGCGCT 1 cut(s) 164
Eco57I CTGAAG 4 cut(s) 515, 705, 983, 989
Eco72I CACGTG 1 cut(s) 608
Eco88I CYCGRG 1 cut(s) 1142
EcoRII CCWGG 3 cut(s) 70, 265, 704
EcoT14I CCWWGG 1 cut(s) 111
EcoT22I ATGCAT 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 3 cut(s) 19, 115, 671
FatI CATG 3 cut(s) 15, 111, 667
FauI CCCGC 1 cut(s) 804
FbaI TGATCA 3 cut(s) 310, 448, 592
FblI GTMKAC 2 cut(s) 559, 951
Fnu4HI GCNGC 7 cut(s) 140, 381, 746, 772, 859, 961, 990
FokI GGATG 3 cut(s) 47, 335, 428
Fsp4HI GCNGC 7 cut(s) 140, 381, 746, 772, 859, 961, 990
FspBI CTAG 4 cut(s) 21, 659, 768, 1082
GlaI GCGC 2 cut(s) 164, 775
GluI GCNGC 7 cut(s) 140, 381, 746, 772, 859, 961, 990
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 2 cut(s) 1131, 1335
HapII CCGG 1 cut(s) 42
HhaI GCGC 2 cut(s) 165, 776
Hin1II CATG 3 cut(s) 19, 115, 671
Hin6I GCGC 2 cut(s) 163, 774
HinP1I GCGC 2 cut(s) 163, 774
HindIII AAGCTT 5 cut(s) 248, 782, 932, 995, 1338
HinfI GANTC 9 cut(s) 101, 302, 332, 837, 882, 1024, 1061, 1234, 1315
HpaII CCGG 1 cut(s) 42
HphI GGTGA 1 cut(s) 1301
Hpy166II GTNNAC 7 cut(s) 306, 560, 673, 694, 758, 952, 1118
Hpy188I TCNGA 8 cut(s) 160, 387, 465, 534, 724, 1002, 1068, 1314
Hpy188III TCNNGA 7 cut(s) 198, 482, 596, 1048, 1115, 1144, 1219
Hpy8I GTNNAC 7 cut(s) 306, 560, 673, 694, 758, 952, 1118
HpyAV CCTTC 2 cut(s) 1142, 1322
HpyCH4III ACNGT 1 cut(s) 621
HpyCH4IV ACGT 5 cut(s) 123, 562, 607, 889, 1031
HpyCH4V TGCA 9 cut(s) 177, 635, 748, 796, 894, 913, 992, 1038, 1193
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 136, 808, 1035
HpyF3I CTNAG 2 cut(s) 251, 1157
HpySE526I ACGT 5 cut(s) 123, 562, 607, 889, 1031
Hsp92II CATG 3 cut(s) 19, 115, 671
HspAI GCGC 2 cut(s) 163, 774
Ksp22I TGATCA 3 cut(s) 310, 448, 592
KspI CCGCGG 1 cut(s) 964
Kzo9I GATC 6 cut(s) 194, 310, 448, 520, 592, 1379
LmnI GCTCC 1 cut(s) 1244
Lsp1109I GCAGC 6 cut(s) 151, 367, 732, 758, 845, 976
MabI ACCWGGT 1 cut(s) 265
MaeI CTAG 4 cut(s) 21, 659, 768, 1082
MaeII ACGT 5 cut(s) 123, 562, 607, 889, 1031
MalI GATC 6 cut(s) 196, 312, 450, 522, 594, 1381
MboI GATC 6 cut(s) 194, 310, 448, 520, 592, 1379
MboII GAAGA 6 cut(s) 16, 521, 982, 985, 1033, 1259
MfeI CAATTG 2 cut(s) 323, 1188
MflI RGATCY 1 cut(s) 194
MlyI GAGTC 3 cut(s) 311, 876, 1070
MmeI TCCRAC 1 cut(s) 607
Mph1103I ATGCAT 1 cut(s) 179
MseI TTAA 5 cut(s) 507, 740, 866, 930, 984
MspA1I CMGCKG 2 cut(s) 858, 963
MspCI CTTAAG 1 cut(s) 929
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 3 cut(s) 72, 267, 706
MunI CAATTG 2 cut(s) 323, 1188
Mva1269I GAATGC 1 cut(s) 715
MvaI CCWGG 3 cut(s) 72, 267, 706
MvnI CGCG 1 cut(s) 963
MwoI GCNNNNNNNGC 4 cut(s) 21, 136, 808, 1035
NcoI CCATGG 1 cut(s) 111
NdeII GATC 6 cut(s) 194, 310, 448, 520, 592, 1379
NlaIII CATG 3 cut(s) 19, 115, 671
NlaIV GGNNCC 1 cut(s) 1246
NmeAIII GCCGAG 1 cut(s) 1273
NsiI ATGCAT 1 cut(s) 179
NspI RCATGY 1 cut(s) 19
PaeR7I CTCGAG 1 cut(s) 1142
PcsI WCGNNNNNNNCGW 1 cut(s) 205
PctI GAATGC 1 cut(s) 715
PfeI GAWTC 6 cut(s) 101, 332, 837, 1024, 1234, 1315
PflMI CCANNNNNTGG 1 cut(s) 408
PinAI ACCGGT 1 cut(s) 41
PkrI GCNGC 7 cut(s) 141, 382, 747, 773, 860, 962, 991
PleI GAGTC 3 cut(s) 310, 876, 1069
PmaCI CACGTG 1 cut(s) 608
PmlI CACGTG 1 cut(s) 608
PpsI GAGTC 3 cut(s) 310, 876, 1069
Ppu21I YACGTR 2 cut(s) 563, 608
Psp1406I AACGTT 1 cut(s) 123
Psp6I CCWGG 3 cut(s) 70, 265, 704
PspCI CACGTG 1 cut(s) 608
PspGI CCWGG 3 cut(s) 70, 265, 704
PspN4I GGNNCC 1 cut(s) 1246
PspPI GGNCC 3 cut(s) 115, 387, 1153
PstI CTGCAG 2 cut(s) 750, 915
PsuI RGATCY 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 858
RsaI GTAC 1 cut(s) 370
RsaNI GTAC 1 cut(s) 369
SacII CCGCGG 1 cut(s) 964
SaqAI TTAA 5 cut(s) 507, 740, 866, 930, 984
SatI GCNGC 7 cut(s) 140, 381, 746, 772, 859, 961, 990
Sau3AI GATC 6 cut(s) 194, 310, 448, 520, 592, 1379
Sau96I GGNCC 3 cut(s) 115, 387, 1153
SchI GAGTC 3 cut(s) 311, 876, 1070
ScrFI CCNGG 3 cut(s) 72, 267, 706
SexAI ACCWGGT 1 cut(s) 265
SfcI CTRYAG 3 cut(s) 746, 911, 1238
Sfr274I CTCGAG 1 cut(s) 1142
Sfr303I CCGCGG 1 cut(s) 964
SgrBI CCGCGG 1 cut(s) 964
SinI GGWCC 3 cut(s) 115, 387, 1153
SlaI CTCGAG 1 cut(s) 1142
SmlI CTYRAG 5 cut(s) 542, 929, 1048, 1142, 1219
SmoI CTYRAG 5 cut(s) 542, 929, 1048, 1142, 1219
SnaBI TACGTA 1 cut(s) 563
SsiI CCGC 3 cut(s) 811, 961, 963
SspI AATATT 2 cut(s) 229, 1183
SspMI CTAG 4 cut(s) 21, 659, 768, 1082
StyD4I CCNGG 3 cut(s) 70, 265, 704
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 621
TaiI ACGT 5 cut(s) 126, 565, 610, 892, 1034
TaqI TCGA 5 cut(s) 199, 736, 1143, 1201, 1378
TaqII GACCGA 1 cut(s) 513
TatI WGTACW 1 cut(s) 368
TauI GCSGC 1 cut(s) 963
TfiI GAWTC 6 cut(s) 101, 332, 837, 1024, 1234, 1315
Tru1I TTAA 5 cut(s) 507, 740, 866, 930, 984
Tru9I TTAA 5 cut(s) 507, 740, 866, 930, 984
TscAI CASTG 4 cut(s) 313, 397, 626, 962
TseI GCWGC 6 cut(s) 139, 380, 745, 771, 858, 989
TspDTI ATGAA 5 cut(s) 17, 51, 332, 843, 1263
TspRI CASTG 4 cut(s) 313, 397, 626, 962
Van91I CCANNNNNTGG 1 cut(s) 408
Vha464I CTTAAG 1 cut(s) 929
VpaK11BI GGWCC 3 cut(s) 115, 387, 1153
XapI RAATTY 4 cut(s) 154, 550, 566, 1374
XceI RCATGY 1 cut(s) 19
XhoI CTCGAG 1 cut(s) 1142
XmiI GTMKAC 2 cut(s) 559, 951
XspI CTAG 4 cut(s) 21, 659, 768, 1082
Zsp2I ATGCAT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.