Rh2AG422600

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013699
Physical Location & Seq
Forward (+)
62670311 .. 62676109
5799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG422600.1

Sequence Viewer

Length: 1530 bp
ATGACCTGCTATTTACCTTTTGAAGATAACATGAAGAGGAAAGACATGTCTAGCACACCACAGGATGAAACCGGTGCTGTTGATTTGATTACACAGGCACCTGGAATTGAAAGAAAGAGAAAGAGAAAGGAATCTAATACCATTGACCAGAACGTTGAGCGACAAAAGCAGCAAACTGCCGAAAATTCAGAGAGCTTGGACAAATGCATTACGCAAGAAGCTGGATCTCGAAAAAAACGAAAGAGAGAAAAATCTAATATTACTGGCAAGAGGGGGAAGCTTAGAAAGAAGCAACCTGGTGGAGTTGTAAATGACATTGAAAAAAGTGTTGAGTCCACTGATCATATCTCACAATTGCCTGAATCTGTCATTCATCACATCCTTTCTCTCATTCGTTGTACAAAAGGTGCCGCTCGGACCAGTGTGTTGTCCAAGAGGTGGAGGGATGTATGGACTTCTTTCTCTGTTTTGACATTTGATCAACGCAAGTTTCAGAAACCAGAGGGAGTTCAAGATAAGAGCAAGAATGAGATGTTTAAGGACTTTATTGATCAGTCTCTTCAGCGCCAACTTGAGCAAAATTTAGGTATACGTAAATTTGAGCTACATTTGACCTCCTATGATCAAGAAATGGCACGTGGTATGGAACAGTGGGTTGGACTTGCAACTGAAAATAACATAAAAGAGCTAGGTCTCCATGTTCACACAAGAGGAAATAATGTTCACAATTTTCCCAGGAATGCGTTTGCTTCAGATACACTAACTCGATTAAGGCTGCAGAGCTGTAAACTGGAAACTAGCTGCTCTGTAAAGCTTCCCCATCTGCAAAAGCTATATTTGCGGGATTGTAATGTTGATGAACAGAGAATCCAGCATATTATTTCCGGCTGCCCTTTAATTGAGGATTTGAGACTCATACGTTGCACGGGGTTGAAGTTTCTGCAGATTTCAAGTCTCCTTAAGCTTGATAGGGTTGAAGTATACCACTGCCGCGGACTGAAGAAGATTGAAGTTAAAGCTGCAAAGCTTCAGACATTTTGGTATTGTGGGAAGAATCGCACGTCTTGCAAACTTATCTTGAGTGGTTGTGAGTCTCTGAAAAGATTGACACTAGAGGACAAAAAAATGACAGACAATCAGTTTCGTGAACTATTCTTTGGCCTTCCTCTCCTCGAGAAGCTGGACCTAAGTAAATGCCTCAAATTGAAAAATATTACAATTGCAAGTCATCGACTAAACAGATTAGTCTTGAGGGGTTGCGAGAATCTACAGGGAGCCGAGATTGATACTCCAAATCTTCTTTCGTTCGAGTATCAAGGTGATAAAATGCCCTTTTCTTTTCTGAATCCTATATCTTTGAAGGAGGCCAAGCTTTCTTTTGGGTCGGCTTCTGCTGGTCAGCTCAAATTCGATCACAATAAAGACGAGCCTTTTTGGTTTAAAAAGTTGCGGGATTTTCTTGCCAAGTTTGATCACAATACAGGCTTCAAACTGATTGTCCGCACTAATAAGGTAACACACTCTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBD Jacalin

Protein Analysis

509

Amino Acids

58.8

Weight (kDa)

9.53

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 194 - 354 4.5e-23 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 216 - 348 7.6e-17 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 361 - 459 1.3e-11 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013699)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g32840 FvH4_6g32840 FvH4_6g32840 FvH4_6g32840
malus_domestica MD09G1197600.v1.1 MD17G1178500.v1.1
prunus_persica Prupe.3G053600_v2.0.a1
pyrus_communis pycom09g11420 pycom17g18740
rosa_chinensis RchiOBHm_Chr2g0142131
rosa_laevigata RLG00000019948
rosa_roxburghii Rroxscaffold_2G00103640
rosa_rugosa Rorug02G0370700
rosa_samantha Rh2AG422600 Rh2BG432800 Rh2CG409500 Rh2DG442500
rosa_wichuraiana Rw2G034630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 14
AccB1I GGYRCC 2 cut(s) 97, 407
AccB7I CCANNNNNTGG 1 cut(s) 438
AccBSI CCGCTC 1 cut(s) 413
AccI GTMKAC 2 cut(s) 589, 981
AccII CGCG 1 cut(s) 993
AciI CCGC 6 cut(s) 411, 841, 991, 993, 1450, 1501
AclI AACGTT 1 cut(s) 153
AclWI GGATC 1 cut(s) 232
AcsI RAATTY 4 cut(s) 184, 580, 596, 1406
AcuI CTGAAG 4 cut(s) 545, 735, 1013, 1019
AcvI CACGTG 1 cut(s) 638
AfaI GTAC 1 cut(s) 400
AfiI CCNNNNNNNGG 1 cut(s) 438
AflII CTTAAG 1 cut(s) 959
AflIII ACRYGT 1 cut(s) 45
AgeI ACCGGT 1 cut(s) 71
AjiI CACGTC 1 cut(s) 1062
AjnI CCWGG 3 cut(s) 100, 295, 734
AjuI GAANNNNNNNTTGG 6 cut(s) 639, 671, 1140, 1172, 1285, 1317
AloI GAACNNNNNNTCC 4 cut(s) 705, 737, 852, 884
Alw26I GTCTC 5 cut(s) 561, 698, 904, 959, 1098
AlwI GGATC 1 cut(s) 232
Ama87I CYCGRG 1 cut(s) 1172
AoxI GGCC 2 cut(s) 1159, 1365
ApeKI GCWGC 5 cut(s) 169, 775, 801, 888, 1019
ApoI RAATTY 4 cut(s) 184, 580, 596, 1406
ArsI GACNNNNNNTTYG 2 cut(s) 1482, 1514
AsiGI ACCGGT 1 cut(s) 71
AspLEI GCGC 1 cut(s) 567
AspS9I GGNCC 2 cut(s) 417, 1183
AsuHPI GGTGA 1 cut(s) 1331
AvaI CYCGRG 1 cut(s) 1172
AvaII GGWCC 2 cut(s) 417, 1183
BanI GGYRCC 2 cut(s) 97, 407
BbrPI CACGTG 1 cut(s) 638
BbvI GCAGC 5 cut(s) 181, 762, 788, 875, 1006
BccI CCATC 1 cut(s) 828
BciT130I CCWGG 3 cut(s) 102, 297, 736
BclI TGATCA 5 cut(s) 340, 478, 550, 622, 1471
BcoDI GTCTC 5 cut(s) 561, 698, 904, 959, 1098
BfaI CTAG 4 cut(s) 51, 689, 798, 1112
BfmI CTRYAG 3 cut(s) 776, 941, 1268
BfoI RGCGCY 1 cut(s) 568
BfrI CTTAAG 1 cut(s) 959
BfuAI ACCTGC 1 cut(s) 14
BisI GCNGC 7 cut(s) 170, 411, 776, 802, 889, 991, 1020
BlsI GCNGC 7 cut(s) 171, 412, 777, 803, 890, 992, 1021
Bme1390I CCNGG 3 cut(s) 102, 297, 736
Bme18I GGWCC 2 cut(s) 417, 1183
BmeT110I CYCGRG 1 cut(s) 1172
BmgBI CACGTC 1 cut(s) 1062
BmgT120I GGNCC 2 cut(s) 417, 1183
BmiI GGNNCC 3 cut(s) 99, 409, 1276
BmrFI CCNGG 3 cut(s) 102, 297, 736
BpuEI CTTGAG 3 cut(s) 593, 1099, 1270
BsaAI YACGTR 2 cut(s) 593, 638
BsaI GGTCTC 1 cut(s) 698
BsaJI CCNNGG 2 cut(s) 734, 991
BsaWI WCCGGW 1 cut(s) 71
Bsc4I CCNNNNNNNGG 1 cut(s) 438
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 3 cut(s) 268, 420, 795
BseBI CCWGG 3 cut(s) 102, 297, 736
BseDI CCNNGG 2 cut(s) 734, 991
BseGI GGATG 3 cut(s) 70, 378, 451
BseLI CCNNNNNNNGG 1 cut(s) 438
BseNI ACTGG 3 cut(s) 268, 420, 795
BseRI GAGGAG 1 cut(s) 1160
BseXI GCAGC 5 cut(s) 181, 762, 788, 875, 1006
Bsh1236I CGCG 1 cut(s) 993
BshFI GGCC 2 cut(s) 1161, 1367
BshNI GGYRCC 2 cut(s) 97, 407
BshTI ACCGGT 1 cut(s) 71
BsiHKCI CYCGRG 1 cut(s) 1172
BsiSI CCGG 2 cut(s) 72, 885
BslI CCNNNNNNNGG 1 cut(s) 438
BsmAI GTCTC 5 cut(s) 561, 698, 904, 959, 1098
BsmI GAATGC 1 cut(s) 745
BsnI GGCC 2 cut(s) 1161, 1367
Bso31I GGTCTC 1 cut(s) 698
BsoBI CYCGRG 1 cut(s) 1172
Bsp1407I TGTACA 1 cut(s) 398
Bsp143I GATC 7 cut(s) 224, 340, 478, 550, 622, 1411, 1471
BspACI CCGC 6 cut(s) 411, 841, 991, 993, 1450, 1501
BspANI GGCC 2 cut(s) 1161, 1367
BspFNI CGCG 1 cut(s) 993
BspLI GGNNCC 3 cut(s) 99, 409, 1276
BspMAI CTGCAG 2 cut(s) 780, 945
BspMI ACCTGC 1 cut(s) 14
BspPI GGATC 1 cut(s) 232
BspT107I GGYRCC 2 cut(s) 97, 407
BspTI CTTAAG 1 cut(s) 959
BspTNI GGTCTC 1 cut(s) 698
BsrBI CCGCTC 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 71
BsrGI TGTACA 1 cut(s) 398
BsrI ACTGG 3 cut(s) 268, 420, 795
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 2 cut(s) 734, 991
BssMI GATC 7 cut(s) 224, 340, 478, 550, 622, 1411, 1471
BssNAI GTATAC 2 cut(s) 590, 982
Bst1107I GTATAC 2 cut(s) 590, 982
Bst2UI CCWGG 3 cut(s) 102, 297, 736
Bst4CI ACNGT 1 cut(s) 651
Bst6I CTCTTC 2 cut(s) 29, 564
BstAFI CTTAAG 1 cut(s) 959
BstAPI GCANNNNNTGC 1 cut(s) 1065
BstAUI TGTACA 1 cut(s) 398
BstBAI YACGTR 2 cut(s) 593, 638
BstDEI CTNAG 2 cut(s) 281, 1187
BstDSI CCRYGG 1 cut(s) 991
BstF5I GGATG 3 cut(s) 70, 378, 451
BstFNI CGCG 1 cut(s) 993
BstH2I RGCGCY 1 cut(s) 568
BstHHI GCGC 1 cut(s) 567
BstKTI GATC 7 cut(s) 227, 343, 481, 553, 625, 1414, 1474
BstMAI GTCTC 5 cut(s) 561, 698, 904, 959, 1098
BstMBI GATC 7 cut(s) 224, 340, 478, 550, 622, 1411, 1471
BstMWI GCNNNNNNNGC 3 cut(s) 166, 838, 1065
BstNI CCWGG 3 cut(s) 102, 297, 736
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 3 cut(s) 100, 295, 734
BstSFI CTRYAG 3 cut(s) 776, 941, 1268
BstSNI TACGTA 1 cut(s) 593
BstUI CGCG 1 cut(s) 993
BstV1I GCAGC 5 cut(s) 181, 762, 788, 875, 1006
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
BstZ17I GTATAC 2 cut(s) 590, 982
BsuRI GGCC 2 cut(s) 1161, 1367
BtgI CCRYGG 1 cut(s) 991
BtrI CACGTC 1 cut(s) 1062
BtsCI GGATG 3 cut(s) 70, 378, 451
BtsI GCAGTG 1 cut(s) 985
BtsIMutI CAGTG 4 cut(s) 336, 427, 656, 985
BveI ACCTGC 1 cut(s) 14
CfoI GCGC 1 cut(s) 567
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 2 cut(s) 417, 1183
Cfr42I CCGCGG 1 cut(s) 994
CsiI ACCWGGT 1 cut(s) 295
Csp6I GTAC 1 cut(s) 399
CspAI ACCGGT 1 cut(s) 71
CviAII CATG 3 cut(s) 31, 46, 698
CviQI GTAC 1 cut(s) 399
DdeI CTNAG 2 cut(s) 281, 1187
DpnI GATC 7 cut(s) 226, 342, 480, 552, 624, 1413, 1473
DpnII GATC 7 cut(s) 224, 340, 478, 550, 622, 1411, 1471
DraI TTTAAA 1 cut(s) 1441
Eam1104I CTCTTC 2 cut(s) 29, 564
EarI CTCTTC 2 cut(s) 29, 564
Eco105I TACGTA 1 cut(s) 593
Eco31I GGTCTC 1 cut(s) 698
Eco47I GGWCC 2 cut(s) 417, 1183
Eco57I CTGAAG 4 cut(s) 545, 735, 1013, 1019
Eco72I CACGTG 1 cut(s) 638
Eco88I CYCGRG 1 cut(s) 1172
EcoRII CCWGG 3 cut(s) 100, 295, 734
EcoT22I ATGCAT 1 cut(s) 209
FaeI CATG 3 cut(s) 34, 49, 701
FatI CATG 3 cut(s) 30, 45, 697
FauI CCCGC 2 cut(s) 834, 1443
FbaI TGATCA 5 cut(s) 340, 478, 550, 622, 1471
FblI GTMKAC 2 cut(s) 589, 981
Fnu4HI GCNGC 7 cut(s) 170, 411, 776, 802, 889, 991, 1020
FokI GGATG 3 cut(s) 77, 365, 458
Fsp4HI GCNGC 7 cut(s) 170, 411, 776, 802, 889, 991, 1020
FspBI CTAG 4 cut(s) 51, 689, 798, 1112
GlaI GCGC 1 cut(s) 566
GluI GCNGC 7 cut(s) 170, 411, 776, 802, 889, 991, 1020
HaeII RGCGCY 1 cut(s) 568
HaeIII GGCC 2 cut(s) 1161, 1367
HapII CCGG 2 cut(s) 72, 885
HhaI GCGC 1 cut(s) 567
Hin1II CATG 3 cut(s) 34, 49, 701
Hin6I GCGC 1 cut(s) 565
HinP1I GCGC 1 cut(s) 565
HindIII AAGCTT 5 cut(s) 278, 812, 962, 1025, 1370
HinfI GANTC 9 cut(s) 131, 332, 362, 867, 912, 1054, 1091, 1264, 1345
HpaII CCGG 2 cut(s) 72, 885
HphI GGTGA 1 cut(s) 1331
Hpy166II GTNNAC 7 cut(s) 336, 590, 703, 724, 788, 982, 1148
Hpy188I TCNGA 7 cut(s) 190, 417, 495, 754, 1032, 1098, 1344
Hpy188III TCNNGA 7 cut(s) 228, 512, 626, 1078, 1145, 1174, 1249
Hpy8I GTNNAC 7 cut(s) 336, 590, 703, 724, 788, 982, 1148
HpyAV CCTTC 2 cut(s) 1172, 1354
HpyCH4III ACNGT 1 cut(s) 651
HpyCH4IV ACGT 5 cut(s) 153, 592, 637, 919, 1061
HpyCH4V TGCA 9 cut(s) 207, 665, 778, 826, 924, 943, 1022, 1068, 1223
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 838, 1065
HpyF3I CTNAG 2 cut(s) 281, 1187
HpySE526I ACGT 5 cut(s) 153, 592, 637, 919, 1061
Hsp92II CATG 3 cut(s) 34, 49, 701
HspAI GCGC 1 cut(s) 565
Ksp22I TGATCA 5 cut(s) 340, 478, 550, 622, 1471
KspI CCGCGG 1 cut(s) 994
Kzo9I GATC 7 cut(s) 224, 340, 478, 550, 622, 1411, 1471
LmnI GCTCC 1 cut(s) 1274
Lsp1109I GCAGC 5 cut(s) 181, 762, 788, 875, 1006
MabI ACCWGGT 1 cut(s) 295
MaeI CTAG 4 cut(s) 51, 689, 798, 1112
MaeII ACGT 5 cut(s) 153, 592, 637, 919, 1061
MaeIII GTNAC 1 cut(s) 1513
MalI GATC 7 cut(s) 226, 342, 480, 552, 624, 1413, 1473
MbiI CCGCTC 1 cut(s) 413
MboI GATC 7 cut(s) 224, 340, 478, 550, 622, 1411, 1471
MboII GAAGA 7 cut(s) 35, 46, 551, 1012, 1015, 1063, 1289
MfeI CAATTG 2 cut(s) 353, 1218
MflI RGATCY 1 cut(s) 224
MlyI GAGTC 3 cut(s) 341, 906, 1100
MmeI TCCRAC 1 cut(s) 637
Mph1103I ATGCAT 1 cut(s) 209
MseI TTAA 7 cut(s) 537, 770, 896, 960, 1014, 1440, 1528
MspA1I CMGCKG 1 cut(s) 993
MspCI CTTAAG 1 cut(s) 959
MspI CCGG 2 cut(s) 72, 885
MspR9I CCNGG 3 cut(s) 102, 297, 736
MunI CAATTG 2 cut(s) 353, 1218
Mva1269I GAATGC 1 cut(s) 745
MvaI CCWGG 3 cut(s) 102, 297, 736
MvnI CGCG 1 cut(s) 993
MwoI GCNNNNNNNGC 3 cut(s) 166, 838, 1065
NdeII GATC 7 cut(s) 224, 340, 478, 550, 622, 1411, 1471
NlaIII CATG 3 cut(s) 34, 49, 701
NlaIV GGNNCC 3 cut(s) 99, 409, 1276
NmeAIII GCCGAG 1 cut(s) 1303
NsiI ATGCAT 1 cut(s) 209
NspI RCATGY 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 1172
PciI ACATGT 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 235
PctI GAATGC 1 cut(s) 745
PfeI GAWTC 6 cut(s) 131, 362, 867, 1054, 1264, 1345
PflMI CCANNNNNTGG 1 cut(s) 438
PinAI ACCGGT 1 cut(s) 71
PkrI GCNGC 7 cut(s) 171, 412, 777, 803, 890, 992, 1021
PleI GAGTC 3 cut(s) 340, 906, 1099
PmaCI CACGTG 1 cut(s) 638
PmlI CACGTG 1 cut(s) 638
PpsI GAGTC 3 cut(s) 340, 906, 1099
Ppu21I YACGTR 2 cut(s) 593, 638
PscI ACATGT 1 cut(s) 45
Psp1406I AACGTT 1 cut(s) 153
Psp6I CCWGG 3 cut(s) 100, 295, 734
PspCI CACGTG 1 cut(s) 638
PspGI CCWGG 3 cut(s) 100, 295, 734
PspN4I GGNNCC 3 cut(s) 99, 409, 1276
PspPI GGNCC 2 cut(s) 417, 1183
PstI CTGCAG 2 cut(s) 780, 945
PsuI RGATCY 1 cut(s) 224
RsaI GTAC 1 cut(s) 400
RsaNI GTAC 1 cut(s) 399
SacII CCGCGG 1 cut(s) 994
SaqAI TTAA 7 cut(s) 537, 770, 896, 960, 1014, 1440, 1528
SatI GCNGC 7 cut(s) 170, 411, 776, 802, 889, 991, 1020
Sau3AI GATC 7 cut(s) 224, 340, 478, 550, 622, 1411, 1471
Sau96I GGNCC 2 cut(s) 417, 1183
SchI GAGTC 3 cut(s) 341, 906, 1100
ScrFI CCNGG 3 cut(s) 102, 297, 736
SexAI ACCWGGT 1 cut(s) 295
SfcI CTRYAG 3 cut(s) 776, 941, 1268
Sfr274I CTCGAG 1 cut(s) 1172
Sfr303I CCGCGG 1 cut(s) 994
SgrBI CCGCGG 1 cut(s) 994
SinI GGWCC 2 cut(s) 417, 1183
SlaI CTCGAG 1 cut(s) 1172
SmlI CTYRAG 5 cut(s) 572, 959, 1078, 1172, 1249
SmoI CTYRAG 5 cut(s) 572, 959, 1078, 1172, 1249
SnaBI TACGTA 1 cut(s) 593
SsiI CCGC 6 cut(s) 411, 841, 991, 993, 1450, 1501
SspI AATATT 2 cut(s) 259, 1213
SspMI CTAG 4 cut(s) 51, 689, 798, 1112
StyD4I CCNGG 3 cut(s) 100, 295, 734
TaaI ACNGT 1 cut(s) 651
TaiI ACGT 5 cut(s) 156, 595, 640, 922, 1064
TaqI TCGA 6 cut(s) 229, 766, 1173, 1231, 1308, 1410
TatI WGTACW 1 cut(s) 398
TauI GCSGC 2 cut(s) 413, 993
TfiI GAWTC 6 cut(s) 131, 362, 867, 1054, 1264, 1345
Tru1I TTAA 7 cut(s) 537, 770, 896, 960, 1014, 1440, 1528
Tru9I TTAA 7 cut(s) 537, 770, 896, 960, 1014, 1440, 1528
TscAI CASTG 4 cut(s) 343, 427, 656, 992
TseI GCWGC 5 cut(s) 169, 775, 801, 888, 1019
TspDTI ATGAA 4 cut(s) 47, 81, 362, 873
TspRI CASTG 4 cut(s) 343, 427, 656, 992
Van91I CCANNNNNTGG 1 cut(s) 438
Vha464I CTTAAG 1 cut(s) 959
VpaK11BI GGWCC 2 cut(s) 417, 1183
XapI RAATTY 4 cut(s) 184, 580, 596, 1406
XceI RCATGY 1 cut(s) 49
XhoI CTCGAG 1 cut(s) 1172
XmiI GTMKAC 2 cut(s) 589, 981
XspI CTAG 4 cut(s) 51, 689, 798, 1112
Zsp2I ATGCAT 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.