Rroxscaffold_2G00110350

Sterile alpha motif (SAM) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014165
Physical Location & Seq
Forward (+)
34611070 .. 34611684
615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00110350.1

Sequence Viewer

Length: 615 bp
ATGGATTGGTTCTCATGGCTATCCAAAACCGGCCTAGAGCCTTCGCTCGTGTACGAGTATGGTCTTTGCTTTGCTCACAATGAGCTCGAGGAAGAAGACATTCTCTACTTCAACCACGAGTTTCTTCAAAGCATGGGAATCTCCATAGCCAAACACAGGCTAGAGATCCTCAAGCTTGCTAGAAAAGAAAAGGGTAGACTAGCAAGTAGCGCTCCGCGGCCCATGACGAGGCTTATAGTGGCGATCAAGAAGACCAAGAGGTGTTTTGCTGAGTTCGTACGGACGTGGACTCGCCGCAGTGAGGATTCTTCAGCTCTAGCACTCGTGCCGAGGCCAAGTAATTACAAGTCGAGGTTTAGAGGGGCCATGCTGAAGAGGAACAAGAAGTTGGGGGCGACCAAACAAGTACAAGGCACCAGGTTGTTGCTCACAAATGGTACAGCTGCTAGTCCTGTGGTGGTTTCCGGGCAGCCTCACGGCGACTGTTTTTCGAGTCCGGTTGTGTACGATGTTCGAAAGGAGGAGGAGATGGACGCAGATGACGACGGGTACTGGTCTACCGGAGTTGAAGAGATCAGGTGGGATACCATGTTCCAGGACTTGAAACCTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_2

Protein Analysis

204

Amino Acids

23.35

Weight (kDa)

8.93

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15760 AT1G80520
fragaria_vesca FvH4_6g29130
malus_domestica MD00G1182100.v1.1 MD17G1217700.v1.1
prunus_persica Prupe.3G093400_v2.0.a1
pyrus_communis pycom17g22200
rosa_chinensis RchiOBHm_Chr2g0134301
rosa_laevigata RLG00000019385
rosa_multiflora Rmu_sc0014586.1_g000006
rosa_roxburghii Rroxscaffold_2G00110350
rosa_rugosa Rorug02G0316000
rosa_samantha Rh2AG368700 Rh2BG374700 Rh2CG352400 Rh2DG392000
rosa_wichuraiana Rw2G030040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 413
AccI GTMKAC 2 cut(s) 196, 557
AccII CGCG 1 cut(s) 217
AciI CCGC 3 cut(s) 215, 217, 295
AclWI GGATC 1 cut(s) 160
AcuI CTGAAG 2 cut(s) 294, 392
AfaI GTAC 6 cut(s) 53, 279, 408, 439, 506, 551
AfeI AGCGCT 1 cut(s) 211
AfiI CCNNNNNNNGG 3 cut(s) 156, 228, 301
AgsI TTSAA 4 cut(s) 112, 128, 569, 604
AjiI CACGTC 1 cut(s) 285
AjnI CCWGG 2 cut(s) 416, 594
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AloI GAACNNNNNNTCC 2 cut(s) 575, 607
AluBI AGCT 4 cut(s) 85, 175, 314, 443
AluI AGCT 4 cut(s) 85, 175, 314, 443
Alw21I GWGCWC 1 cut(s) 87
AlwI GGATC 1 cut(s) 160
Ama87I CYCGRG 1 cut(s) 86
Aor51HI AGCGCT 1 cut(s) 211
AoxI GGCC 4 cut(s) 31, 218, 332, 363
ApeKI GCWGC 2 cut(s) 443, 469
Asp700I GAANNNNTTC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 219, 363
AsuC2I CCSGG 1 cut(s) 466
AsuII TTCGAA 1 cut(s) 514
AvaI CYCGRG 1 cut(s) 86
BanI GGYRCC 1 cut(s) 413
BanII GRGCYC 1 cut(s) 87
BauI CACGAG 3 cut(s) 47, 116, 323
BbsI GAAGAC 2 cut(s) 102, 257
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 2 cut(s) 430, 481
BccI CCATC 1 cut(s) 523
BceAI ACGGC 1 cut(s) 493
BciT130I CCWGG 2 cut(s) 418, 596
BciVI GTATCC 1 cut(s) 577
BcnI CCSGG 1 cut(s) 466
BfaI CTAG 6 cut(s) 35, 161, 180, 200, 317, 447
BfoI RGCGCY 1 cut(s) 213
BfuI GTATCC 1 cut(s) 577
BisI GCNGC 4 cut(s) 218, 295, 444, 470
BlsI GCNGC 4 cut(s) 219, 296, 445, 471
Bme1390I CCNGG 3 cut(s) 418, 466, 596
BmeT110I CYCGRG 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 285
BmgT120I GGNCC 2 cut(s) 219, 363
BmiI GGNNCC 2 cut(s) 364, 415
BmrFI CCNGG 3 cut(s) 418, 466, 596
BpiI GAAGAC 2 cut(s) 102, 257
Bpu14I TTCGAA 1 cut(s) 514
BpuEI CTTGAG 1 cut(s) 155
BpuMI CCSGG 1 cut(s) 466
BsaJI CCNNGG 2 cut(s) 215, 329
BsaWI WCCGGW 2 cut(s) 496, 560
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 228, 301
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 1 cut(s) 557
BseBI CCWGG 2 cut(s) 418, 596
BseDI CCNNGG 2 cut(s) 215, 329
BseLI CCNNNNNNNGG 3 cut(s) 156, 228, 301
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 557
BseRI GAGGAG 2 cut(s) 536, 539
BseXI GCAGC 2 cut(s) 430, 481
Bsh1236I CGCG 1 cut(s) 217
BshFI GGCC 4 cut(s) 33, 220, 334, 365
BshNI GGYRCC 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 4 cut(s) 30, 465, 497, 561
BsiWI CGTACG 1 cut(s) 277
BslI CCNNNNNNNGG 3 cut(s) 156, 228, 301
BsnI GGCC 4 cut(s) 33, 220, 334, 365
BsoBI CYCGRG 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 514
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 3 cut(s) 165, 243, 573
BspACI CCGC 3 cut(s) 215, 217, 295
BspANI GGCC 4 cut(s) 33, 220, 334, 365
BspCNI CTCAG 1 cut(s) 262
BspFNI CGCG 1 cut(s) 217
BspLI GGNNCC 2 cut(s) 364, 415
BspPI GGATC 1 cut(s) 160
BspT104I TTCGAA 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 29
BsrI ACTGG 1 cut(s) 557
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 2 cut(s) 215, 329
BssMI GATC 3 cut(s) 165, 243, 573
BssSI CACGAG 3 cut(s) 47, 116, 323
Bst2BI CACGAG 3 cut(s) 47, 116, 323
Bst2UI CCWGG 2 cut(s) 418, 596
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 2 cut(s) 368, 564
BstBI TTCGAA 1 cut(s) 514
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 1 cut(s) 270
BstDSI CCRYGG 1 cut(s) 215
BstFNI CGCG 1 cut(s) 217
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 3 cut(s) 168, 246, 576
BstMBI GATC 3 cut(s) 165, 243, 573
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 2 cut(s) 418, 596
BstSCI CCNGG 3 cut(s) 416, 464, 594
BstUI CGCG 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 430, 481
BstV2I GAAGAC 2 cut(s) 102, 257
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
BsuI GTATCC 1 cut(s) 577
BsuRI GGCC 4 cut(s) 33, 220, 334, 365
BtgI CCRYGG 1 cut(s) 215
BtrI CACGTC 1 cut(s) 285
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 304
Cac8I GCNNGC 1 cut(s) 177
CfoI GCGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 2 cut(s) 219, 363
Cfr42I CCGCGG 1 cut(s) 218
CseI GACGC 1 cut(s) 542
CsiI ACCWGGT 1 cut(s) 416
Csp6I GTAC 6 cut(s) 52, 278, 407, 438, 505, 550
CviAII CATG 5 cut(s) 15, 133, 223, 367, 589
CviQI GTAC 6 cut(s) 52, 278, 407, 438, 505, 550
DdeI CTNAG 1 cut(s) 270
DpnI GATC 3 cut(s) 167, 245, 575
DpnII GATC 3 cut(s) 165, 243, 573
Eam1104I CTCTTC 2 cut(s) 368, 564
EarI CTCTTC 2 cut(s) 368, 564
Ecl136II GAGCTC 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 87
Eco47III AGCGCT 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 85
Eco57I CTGAAG 2 cut(s) 294, 392
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 85
EcoRII CCWGG 2 cut(s) 416, 594
EcoT38I GRGCYC 1 cut(s) 87
FaeI CATG 5 cut(s) 18, 136, 226, 370, 592
FaiI YATR 8 cut(s) 16, 60, 134, 146, 224, 236, 368, 590
FatI CATG 5 cut(s) 14, 132, 222, 366, 588
FblI GTMKAC 2 cut(s) 196, 557
Fnu4HI GCNGC 4 cut(s) 218, 295, 444, 470
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 4 cut(s) 218, 295, 444, 470
FspBI CTAG 6 cut(s) 35, 161, 180, 200, 317, 447
GlaI GCGC 1 cut(s) 211
GluI GCNGC 4 cut(s) 218, 295, 444, 470
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 4 cut(s) 33, 220, 334, 365
HapII CCGG 4 cut(s) 30, 465, 497, 561
HgaI GACGC 1 cut(s) 542
HhaI GCGC 1 cut(s) 212
Hin1II CATG 5 cut(s) 18, 136, 226, 370, 592
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 4 cut(s) 138, 289, 305, 493
HpaII CCGG 4 cut(s) 30, 465, 497, 561
Hpy166II GTNNAC 5 cut(s) 52, 197, 288, 505, 558
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 5 cut(s) 52, 197, 288, 505, 558
Hpy99I CGWCG 1 cut(s) 548
HpyAV CCTTC 1 cut(s) 51
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4IV ACGT 1 cut(s) 284
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 1 cut(s) 270
HpySE526I ACGT 1 cut(s) 284
Hsp92II CATG 5 cut(s) 18, 136, 226, 370, 592
HspAI GCGC 1 cut(s) 210
KspI CCGCGG 1 cut(s) 218
Kzo9I GATC 3 cut(s) 165, 243, 573
LmnI GCTCC 1 cut(s) 217
Lsp1109I GCAGC 2 cut(s) 430, 481
MabI ACCWGGT 1 cut(s) 416
MaeI CTAG 6 cut(s) 35, 161, 180, 200, 317, 447
MaeII ACGT 1 cut(s) 284
MalI GATC 3 cut(s) 167, 245, 575
MboI GATC 3 cut(s) 165, 243, 573
MboII GAAGA 7 cut(s) 104, 107, 116, 262, 300, 385, 581
MflI RGATCY 1 cut(s) 165
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 1 cut(s) 340
MlyI GAGTC 2 cut(s) 283, 502
MroXI GAANNNNTTC 1 cut(s) 99
MspA1I CMGCKG 2 cut(s) 217, 443
MspI CCGG 4 cut(s) 30, 465, 497, 561
MspR9I CCNGG 3 cut(s) 418, 466, 596
MvaI CCWGG 2 cut(s) 418, 596
MvnI CGCG 1 cut(s) 217
MwoI GCNNNNNNNGC 1 cut(s) 209
NciI CCSGG 1 cut(s) 466
NdeII GATC 3 cut(s) 165, 243, 573
NlaIII CATG 5 cut(s) 18, 136, 226, 370, 592
NlaIV GGNNCC 2 cut(s) 364, 415
NmeAIII GCCGAG 1 cut(s) 354
NspV TTCGAA 1 cut(s) 514
PaeR7I CTCGAG 1 cut(s) 86
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PdmI GAANNNNTTC 1 cut(s) 99
PfeI GAWTC 2 cut(s) 138, 305
Pfl23II CGTACG 1 cut(s) 277
PfoI TCCNGGA 1 cut(s) 594
PkrI GCNGC 4 cut(s) 219, 296, 445, 471
PleI GAGTC 2 cut(s) 283, 501
PpsI GAGTC 2 cut(s) 283, 501
Psp124BI GAGCTC 1 cut(s) 87
Psp6I CCWGG 2 cut(s) 416, 594
PspGI CCWGG 2 cut(s) 416, 594
PspLI CGTACG 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 364, 415
PspPI GGNCC 2 cut(s) 219, 363
PspXI VCTCGAGB 1 cut(s) 86
PsuI RGATCY 1 cut(s) 165
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 6 cut(s) 53, 279, 408, 439, 506, 551
RsaNI GTAC 6 cut(s) 52, 278, 407, 438, 505, 550
SacI GAGCTC 1 cut(s) 87
SacII CCGCGG 1 cut(s) 218
SatI GCNGC 4 cut(s) 218, 295, 444, 470
Sau3AI GATC 3 cut(s) 165, 243, 573
Sau96I GGNCC 2 cut(s) 219, 363
SchI GAGTC 2 cut(s) 283, 502
ScrFI CCNGG 3 cut(s) 418, 466, 596
SduI GDGCHC 1 cut(s) 87
SexAI ACCWGGT 1 cut(s) 416
Sfr274I CTCGAG 1 cut(s) 86
Sfr303I CCGCGG 1 cut(s) 218
SfuI TTCGAA 1 cut(s) 514
SgrBI CCGCGG 1 cut(s) 218
SlaI CTCGAG 1 cut(s) 86
SmlI CTYRAG 2 cut(s) 86, 170
SmoI CTYRAG 2 cut(s) 86, 170
Sse9I AATT 1 cut(s) 340
SsiI CCGC 3 cut(s) 215, 217, 295
SspMI CTAG 6 cut(s) 35, 161, 180, 200, 317, 447
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 3 cut(s) 416, 464, 594
TaaI ACNGT 1 cut(s) 485
TaiI ACGT 1 cut(s) 287
TaqI TCGA 4 cut(s) 87, 350, 491, 514
TasI AATT 1 cut(s) 340
TatI WGTACW 1 cut(s) 406
TauI GCSGC 2 cut(s) 220, 297
TfiI GAWTC 2 cut(s) 138, 305
TscAI CASTG 1 cut(s) 304
TseI GCWGC 2 cut(s) 443, 469
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 1 cut(s) 304
XhoI CTCGAG 1 cut(s) 86
XmiI GTMKAC 2 cut(s) 196, 557
XmnI GAANNNNTTC 1 cut(s) 99
XspI CTAG 6 cut(s) 35, 161, 180, 200, 317, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.