Rroxscaffold_2G00113660

bromo domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005410
Physical Location & Seq
Forward (+)
39368919 .. 39376365
7447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00113660.1

Sequence Viewer

Length: 378 bp
ATGATAACTGAAAAGCTTGATGCTGGGATGCAAACTGGTACAAAAATGATTACTTTGGCGCAACATGAAGATGATTCTGGAATGGAGGTTGATACCCCTTCTTCAACCGGGACGGATCAGCAGCACCAGCACTACAATGTTGCCAATATCAAGCAAGAGTTAGTTACGGTTTTAAAGAAGGTAATGAAAATGGATGCTGCTGAACCTTTTAATGCCCCCGTTAATCCCGAAGCTTTAGGAATACCTGATTATTTTGATGTGATAGATACACCAATGGACTTTGGAACAATATGCAGCAACCTTGAACTGGGTGTTAAATATAGAAATTCGGAGGATGTATACAAAGATGTTCAGTATATTTGGGACAACTCGTTATAA

Protein Analysis

125

Amino Acids

14.0

Weight (kDa)

4.34

Isoelectric Point (pI)

30.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bromodomain PF00439 56 - 124 4.6e-14 Bromodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 376
AccI GTMKAC 1 cut(s) 339
AclWI GGATC 1 cut(s) 123
AcsI RAATTY 1 cut(s) 325
AfaI GTAC 1 cut(s) 40
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 2 cut(s) 105, 305
AluBI AGCT 2 cut(s) 16, 233
AluI AGCT 2 cut(s) 16, 233
AlwI GGATC 1 cut(s) 123
ApeKI GCWGC 3 cut(s) 121, 197, 294
ApoI RAATTY 1 cut(s) 325
AspLEI GCGC 1 cut(s) 61
AsuC2I CCSGG 1 cut(s) 109
BbvI GCAGC 3 cut(s) 133, 184, 306
BcnI CCSGG 1 cut(s) 109
BisI GCNGC 3 cut(s) 122, 198, 295
BlsI GCNGC 3 cut(s) 123, 199, 296
Bme1390I CCNGG 1 cut(s) 109
BmrFI CCNGG 1 cut(s) 109
BmrI ACTGGG 1 cut(s) 317
BmsI GCATC 3 cut(s) 10, 18, 184
BmuI ACTGGG 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 109
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 2 cut(s) 40, 312
BseGI GGATG 3 cut(s) 33, 199, 340
BseLI CCNNNNNNNGG 1 cut(s) 307
BseNI ACTGG 2 cut(s) 40, 312
BseXI GCAGC 3 cut(s) 133, 184, 306
BseYI CCCAGC 1 cut(s) 23
BsiSI CCGG 1 cut(s) 108
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 124
Bsp143I GATC 1 cut(s) 115
BspPI GGATC 1 cut(s) 123
BsrI ACTGG 2 cut(s) 40, 312
BssMI GATC 1 cut(s) 115
BssNAI GTATAC 1 cut(s) 340
Bst1107I GTATAC 1 cut(s) 340
Bst4CI ACNGT 1 cut(s) 169
BstF5I GGATG 3 cut(s) 33, 199, 340
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 1 cut(s) 118
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 1 cut(s) 127
BstSCI CCNGG 1 cut(s) 107
BstV1I GCAGC 3 cut(s) 133, 184, 306
BstZ17I GTATAC 1 cut(s) 340
BtsCI GGATG 3 cut(s) 33, 199, 340
CfoI GCGC 1 cut(s) 61
Csp6I GTAC 1 cut(s) 39
CviAII CATG 1 cut(s) 65
CviJI RGCY 2 cut(s) 16, 233
CviKI_1 RGCY 2 cut(s) 16, 233
CviQI GTAC 1 cut(s) 39
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
DraI TTTAAA 1 cut(s) 174
FaeI CATG 1 cut(s) 68
FaiI YATR 6 cut(s) 66, 292, 321, 340, 357, 376
FaqI GGGAC 1 cut(s) 124
FatI CATG 1 cut(s) 64
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 3 cut(s) 122, 198, 295
FokI GGATG 3 cut(s) 40, 206, 347
Fsp4HI GCNGC 3 cut(s) 122, 198, 295
GlaI GCGC 1 cut(s) 60
GluI GCNGC 3 cut(s) 122, 198, 295
GsaI CCCAGC 1 cut(s) 27
HapII CCGG 1 cut(s) 108
HhaI GCGC 1 cut(s) 61
Hin1II CATG 1 cut(s) 68
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HindIII AAGCTT 2 cut(s) 14, 231
HinfI GANTC 1 cut(s) 74
HpaII CCGG 1 cut(s) 108
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 2 cut(s) 78, 227
Hpy8I GTNNAC 1 cut(s) 340
HpyAV CCTTC 2 cut(s) 108, 172
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4V TGCA 2 cut(s) 31, 294
HpyF10VI GCNNNNNNNGC 1 cut(s) 127
Hsp92II CATG 1 cut(s) 68
HspAI GCGC 1 cut(s) 59
Kzo9I GATC 1 cut(s) 115
LpnPI CCDG 7 cut(s) 9, 21, 63, 121, 140, 258, 293
Lsp1109I GCAGC 3 cut(s) 133, 184, 306
LweI GCATC 3 cut(s) 10, 18, 184
MaeIII GTNAC 1 cut(s) 163
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 2 cut(s) 80, 93
MluCI AATT 1 cut(s) 325
MnlI CCTC 2 cut(s) 79, 325
MseI TTAA 4 cut(s) 173, 210, 222, 315
MslI CAYNNNNRTG 2 cut(s) 69, 135
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 109
MwoI GCNNNNNNNGC 1 cut(s) 127
NciI CCSGG 1 cut(s) 109
NdeII GATC 1 cut(s) 115
NlaIII CATG 1 cut(s) 68
PfeI GAWTC 1 cut(s) 74
PkrI GCNGC 3 cut(s) 123, 199, 296
PsiI TTATAA 1 cut(s) 376
PspFI CCCAGC 1 cut(s) 23
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
RseI CAYNNNNRTG 2 cut(s) 69, 135
SaqAI TTAA 4 cut(s) 173, 210, 222, 315
SatI GCNGC 3 cut(s) 122, 198, 295
Sau3AI GATC 1 cut(s) 115
ScrFI CCNGG 1 cut(s) 109
SetI ASST 7 cut(s) 18, 90, 183, 208, 235, 247, 303
SfaNI GCATC 3 cut(s) 10, 18, 184
SmiMI CAYNNNNRTG 2 cut(s) 69, 135
Sse9I AATT 1 cut(s) 325
StyD4I CCNGG 1 cut(s) 107
TaaI ACNGT 1 cut(s) 169
TasI AATT 1 cut(s) 325
TfiI GAWTC 1 cut(s) 74
Tru1I TTAA 4 cut(s) 173, 210, 222, 315
Tru9I TTAA 4 cut(s) 173, 210, 222, 315
TseI GCWGC 3 cut(s) 121, 197, 294
TspDTI ATGAA 2 cut(s) 81, 200
TspGWI ACGGA 1 cut(s) 128
XapI RAATTY 1 cut(s) 325
XmiI GTMKAC 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.