Rroxscaffold_2G00119930

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017823
Physical Location & Seq
Forward (+)
52040588 .. 52042247
1660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00119930.1

Sequence Viewer

Length: 483 bp
ATGCAGAAATGCAAACACAGTTCTGGTCTTGAGCGCATATGGGATCGTGGAGAGCAGTATTGTAACATCACTCATAGAGATTTAGTACTTGATAGAATTGGATGGCATGGGAAAGACCGGTGGGCGGCCATTGCGGATTTCAATGGAGAAGACATGAGAAGAGTTGTCGGGGGCGTTCAAAGAGACATCCCAGCTCTCCATTTTGAGCCCAGGGTAAAACTTCTTTGCTTGGTGAGTGAAGCTGATTGTATGGTTAGAGAAATGGAAGAGAAGGGAATGGTTCCTGATGCCATTTCGTATAATATCCTGATTAATGGCTCAGCTCAGGTGCTAAGTAGTTTAGCTTATTGTGATTTGGACTCGGAAATCAATCATCTATGGTACACTCATTTCGTGGCAAGCTTCACTCTCCACTACTTGCACTCCTATAGTCCTTACACCGTTGAAGAGTCAGGAACTCTAATTCCTAAAGATCGAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

18.36

Weight (kDa)

5.71

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 78 - 107 3.3e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 125, 134
AclWI GGATC 1 cut(s) 51
AcoI YGGCCR 1 cut(s) 126
AfaI GTAC 2 cut(s) 87, 383
AfiI CCNNNNNNNGG 1 cut(s) 124
AgeI ACCGGT 1 cut(s) 117
AgsI TTSAA 3 cut(s) 142, 179, 446
AjnI CCWGG 1 cut(s) 209
AloI GAACNNNNNNTCC 2 cut(s) 448, 480
AluBI AGCT 5 cut(s) 194, 242, 323, 344, 402
AluI AGCT 5 cut(s) 194, 242, 323, 344, 402
Alw26I GTCTC 1 cut(s) 177
AlwI GGATC 1 cut(s) 51
AoxI GGCC 1 cut(s) 126
AseI ATTAAT 1 cut(s) 312
AsiGI ACCGGT 1 cut(s) 117
AspLEI GCGC 1 cut(s) 36
AsuHPI GGTGA 1 cut(s) 244
BanII GRGCYC 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 156
BccI CCATC 1 cut(s) 96
BciT130I CCWGG 1 cut(s) 211
BcoDI GTCTC 1 cut(s) 177
BfmI CTRYAG 1 cut(s) 427
BisI GCNGC 1 cut(s) 126
BlpI GCTNAGC 1 cut(s) 319
BlsI GCNGC 1 cut(s) 127
BmcAI AGTACT 1 cut(s) 87
Bme1390I CCNGG 1 cut(s) 211
BmiI GGNNCC 1 cut(s) 282
BmrFI CCNGG 1 cut(s) 211
BmsI GCATC 1 cut(s) 277
BpiI GAAGAC 1 cut(s) 156
Bpu10I CCTNAGC 1 cut(s) 324
Bpu1102I GCTNAGC 1 cut(s) 319
BpuEI CTTGAG 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 209, 210
BsaWI WCCGGW 1 cut(s) 117
BsaXI ACNNNNNCTCC 2 cut(s) 407, 437
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse118I RCCGGY 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 129
BseBI CCWGG 1 cut(s) 211
BseDI CCNNGG 2 cut(s) 209, 210
BseGI GGATG 2 cut(s) 107, 186
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMI GCAATG 1 cut(s) 129
BseMII CTCAG 2 cut(s) 333, 338
BseYI CCCAGC 1 cut(s) 190
BshFI GGCC 1 cut(s) 128
BshTI ACCGGT 1 cut(s) 117
BsiSI CCGG 1 cut(s) 118
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 177
BsnI GGCC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 2 cut(s) 43, 472
Bsp1720I GCTNAGC 1 cut(s) 319
BspACI CCGC 2 cut(s) 125, 134
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 2 cut(s) 332, 337
BspLI GGNNCC 1 cut(s) 282
BspPI GGATC 1 cut(s) 51
BsrDI GCAATG 1 cut(s) 129
BsrFI RCCGGY 1 cut(s) 117
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 2 cut(s) 209, 210
BssMI GATC 2 cut(s) 43, 472
Bst2UI CCWGG 1 cut(s) 211
Bst4CI ACNGT 2 cut(s) 20, 442
Bst6I CTCTTC 3 cut(s) 154, 261, 441
BstC8I GCNNGC 1 cut(s) 400
BstDEI CTNAG 3 cut(s) 319, 324, 332
BstF5I GGATG 2 cut(s) 107, 186
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 2 cut(s) 46, 475
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 2 cut(s) 43, 472
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 1 cut(s) 211
BstSCI CCNGG 1 cut(s) 209
BstSFI CTRYAG 1 cut(s) 427
BstV2I GAAGAC 1 cut(s) 156
BsuRI GGCC 1 cut(s) 128
BtsCI GGATG 2 cut(s) 107, 186
Cac8I GCNNGC 1 cut(s) 400
CfoI GCGC 1 cut(s) 36
Cfr10I RCCGGY 1 cut(s) 117
Csp6I GTAC 2 cut(s) 86, 382
CspAI ACCGGT 1 cut(s) 117
CviAII CATG 2 cut(s) 107, 154
CviJI RGCY 8 cut(s) 128, 194, 208, 242, 318, 323, 344, 402
CviKI_1 RGCY 8 cut(s) 128, 194, 208, 242, 318, 323, 344, 402
CviQI GTAC 2 cut(s) 86, 382
DdeI CTNAG 3 cut(s) 319, 324, 332
DpnI GATC 2 cut(s) 45, 474
DpnII GATC 2 cut(s) 43, 472
EaeI YGGCCR 1 cut(s) 126
Eam1104I CTCTTC 3 cut(s) 154, 261, 441
EarI CTCTTC 3 cut(s) 154, 261, 441
Eco24I GRGCYC 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 209
EcoT38I GRGCYC 1 cut(s) 210
FaeI CATG 2 cut(s) 110, 157
FaiI YATR 9 cut(s) 38, 40, 75, 108, 155, 251, 300, 379, 429
FatI CATG 2 cut(s) 106, 153
FauNDI CATATG 1 cut(s) 38
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 2 cut(s) 114, 173
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 1 cut(s) 126
GlaI GCGC 1 cut(s) 35
GluI GCNGC 1 cut(s) 126
GsaI CCCAGC 1 cut(s) 194
HaeIII GGCC 1 cut(s) 128
HapII CCGG 1 cut(s) 118
HhaI GCGC 1 cut(s) 36
Hin1II CATG 2 cut(s) 110, 157
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 2 cut(s) 359, 449
HpaII CCGG 1 cut(s) 118
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 1 cut(s) 384
Hpy188I TCNGA 1 cut(s) 364
Hpy188III TCNNGA 4 cut(s) 29, 284, 307, 453
Hpy8I GTNNAC 1 cut(s) 384
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 2 cut(s) 20, 442
HpyCH4V TGCA 3 cut(s) 4, 12, 421
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 3 cut(s) 319, 324, 332
Hsp92II CATG 2 cut(s) 110, 157
HspAI GCGC 1 cut(s) 34
Kzo9I GATC 2 cut(s) 43, 472
LpnPI CCDG 9 cut(s) 9, 131, 196, 204, 223, 297, 311, 320, 438
LweI GCATC 1 cut(s) 277
MaeIII GTNAC 1 cut(s) 62
MalI GATC 2 cut(s) 45, 474
MboI GATC 2 cut(s) 43, 472
MboII GAAGA 4 cut(s) 161, 171, 278, 458
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 2 cut(s) 96, 462
MlyI GAGTC 2 cut(s) 353, 458
MnlI CCTC 1 cut(s) 470
MseI TTAA 1 cut(s) 312
MspI CCGG 1 cut(s) 118
MspR9I CCNGG 1 cut(s) 211
MvaI CCWGG 1 cut(s) 211
MwoI GCNNNNNNNGC 1 cut(s) 131
NdeI CATATG 1 cut(s) 38
NdeII GATC 2 cut(s) 43, 472
NlaIII CATG 2 cut(s) 110, 157
NlaIV GGNNCC 1 cut(s) 282
PasI CCCWGGG 1 cut(s) 210
PinAI ACCGGT 1 cut(s) 117
PkrI GCNGC 1 cut(s) 127
PleI GAGTC 2 cut(s) 353, 457
PpsI GAGTC 2 cut(s) 353, 457
PshBI ATTAAT 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 209
PspFI CCCAGC 1 cut(s) 190
PspGI CCWGG 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 282
RsaI GTAC 2 cut(s) 87, 383
RsaNI GTAC 2 cut(s) 86, 382
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 2 cut(s) 43, 472
ScaI AGTACT 1 cut(s) 87
SchI GAGTC 2 cut(s) 353, 458
ScrFI CCNGG 1 cut(s) 211
SduI GDGCHC 1 cut(s) 210
SetI ASST 6 cut(s) 196, 244, 325, 330, 346, 404
SfaNI GCATC 1 cut(s) 277
SfcI CTRYAG 1 cut(s) 427
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 2 cut(s) 96, 462
SsiI CCGC 2 cut(s) 125, 134
StyD4I CCNGG 1 cut(s) 209
TaaI ACNGT 2 cut(s) 20, 442
TaqI TCGA 1 cut(s) 475
TasI AATT 2 cut(s) 96, 462
TatI WGTACW 1 cut(s) 85
TauI GCSGC 1 cut(s) 128
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
VspI ATTAAT 1 cut(s) 312
ZrmI AGTACT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.