Rroxscaffold_2G00120030

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017818
Physical Location & Seq
Forward (+)
52114924 .. 52115442
519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00120030.1

Sequence Viewer

Length: 249 bp
ATGGAGGACCTCGGCAGCAATATTGAAAACATTCAATCTACATTAGATGGGGGAATGGAGGATGTTAACTCTTCAATGGGACTAGTCAACAACAACGTTTTAGACAATGCACCGTGCTCGCTTGCTGTCTTTGTTGACCGCGGGCTCAAGAGCACATTTATAGAGTCGTCCACCAACAACAACGCCACAACCACCGCCACCATTACGCCATGCTCTTCGCTGGTGGGCCGGATGACCGGGAGGCATTAG

Protein Analysis

82

Amino Acids

8.64

Weight (kDa)

4.35

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHX17_2nd PF23256 15 - 52 5e-06 Plant cation/H(+) antiporter domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017818)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0028811
rosa_laevigata RLG00000018790 RLG00000034794
rosa_roxburghii Rroxscaffold_1G00051090 Rroxscaffold_2G00120030
rosa_samantha Rh1AG072100 Rh1CG071500 Rh2AG308900 Rh2AG434900 Rh2CG420900 Rh5BG200900
rosa_wichuraiana Rw2G024900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 141
AciI CCGC 3 cut(s) 139, 141, 195
AclI AACGTT 1 cut(s) 96
AgsI TTSAA 3 cut(s) 26, 35, 75
AhlI ACTAGT 1 cut(s) 82
Alw21I GWGCWC 2 cut(s) 119, 155
AoxI GGCC 1 cut(s) 226
ApeKI GCWGC 1 cut(s) 15
Asp700I GAANNNNTTC 1 cut(s) 30
AspS9I GGNCC 2 cut(s) 7, 226
AsuC2I CCSGG 1 cut(s) 238
AvaII GGWCC 1 cut(s) 7
BanII GRGCYC 1 cut(s) 147
Bbv12I GWGCWC 2 cut(s) 119, 155
BbvI GCAGC 1 cut(s) 27
BccI CCATC 1 cut(s) 41
BcgI CGANNNNNNTGC 2 cut(s) 99, 133
BcnI CCSGG 1 cut(s) 238
BcuI ACTAGT 1 cut(s) 82
BfaI CTAG 1 cut(s) 83
BisI GCNGC 1 cut(s) 16
BlsI GCNGC 1 cut(s) 17
Bme1390I CCNGG 1 cut(s) 238
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 7, 226
BmrFI CCNGG 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 131
BpuMI CCSGG 1 cut(s) 238
BsaJI CCNNGG 2 cut(s) 10, 139
BseDI CCNNGG 2 cut(s) 10, 139
BseGI GGATG 2 cut(s) 67, 237
BseXI GCAGC 1 cut(s) 27
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 1 cut(s) 228
BsiHKAI GWGCWC 2 cut(s) 119, 155
BsiSI CCGG 2 cut(s) 229, 237
BslFI GGGAC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 93
BsnI GGCC 1 cut(s) 228
Bsp1286I GDGCHC 3 cut(s) 119, 147, 155
BspACI CCGC 3 cut(s) 139, 141, 195
BspANI GGCC 1 cut(s) 228
BspFNI CGCG 1 cut(s) 141
BspQI GCTCTTC 1 cut(s) 220
BssECI CCNNGG 2 cut(s) 10, 139
Bst4CI ACNGT 1 cut(s) 114
Bst6I CTCTTC 2 cut(s) 76, 220
BstC8I GCNNGC 3 cut(s) 119, 123, 143
BstDSI CCRYGG 1 cut(s) 139
BstF5I GGATG 2 cut(s) 67, 237
BstFNI CGCG 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 236
BstUI CGCG 1 cut(s) 141
BstV1I GCAGC 1 cut(s) 27
BsuRI GGCC 1 cut(s) 228
BtgI CCRYGG 1 cut(s) 139
BtsCI GGATG 2 cut(s) 67, 237
Cac8I GCNNGC 3 cut(s) 119, 123, 143
Cfr13I GGNCC 2 cut(s) 7, 226
Cfr42I CCGCGG 1 cut(s) 142
CviAII CATG 1 cut(s) 210
CviJI RGCY 2 cut(s) 145, 228
CviKI_1 RGCY 2 cut(s) 145, 228
Eam1104I CTCTTC 2 cut(s) 76, 220
EarI CTCTTC 2 cut(s) 76, 220
Eco24I GRGCYC 1 cut(s) 147
Eco47I GGWCC 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 7
EcoT38I GRGCYC 1 cut(s) 147
FaeI CATG 1 cut(s) 213
FaiI YATR 2 cut(s) 161, 211
FaqI GGGAC 1 cut(s) 93
FatI CATG 1 cut(s) 209
FauI CCCGC 1 cut(s) 134
Fnu4HI GCNGC 1 cut(s) 16
FokI GGATG 2 cut(s) 74, 244
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 1 cut(s) 16
FspBI CTAG 1 cut(s) 83
GluI GCNGC 1 cut(s) 16
HaeIII GGCC 1 cut(s) 228
HapII CCGG 2 cut(s) 229, 237
Hin1II CATG 1 cut(s) 213
HincII GTYRAC 3 cut(s) 67, 88, 136
HindII GTYRAC 3 cut(s) 67, 88, 136
HinfI GANTC 1 cut(s) 164
HpaI GTTAAC 1 cut(s) 67
HpaII CCGG 2 cut(s) 229, 237
Hpy166II GTNNAC 4 cut(s) 67, 88, 136, 171
Hpy188III TCNNGA 1 cut(s) 148
Hpy8I GTNNAC 4 cut(s) 67, 88, 136, 171
HpyCH4III ACNGT 1 cut(s) 114
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 1 cut(s) 110
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 1 cut(s) 213
KspAI GTTAAC 1 cut(s) 67
KspI CCGCGG 1 cut(s) 142
LguI GCTCTTC 1 cut(s) 220
LpnPI CCDG 2 cut(s) 206, 242
Lsp1109I GCAGC 1 cut(s) 27
MaeI CTAG 1 cut(s) 83
MaeII ACGT 1 cut(s) 96
MboII GAAGA 2 cut(s) 63, 207
MhlI GDGCHC 3 cut(s) 119, 147, 155
MlyI GAGTC 1 cut(s) 173
MnlI CCTC 3 cut(s) 20, 52, 234
MroXI GAANNNNTTC 1 cut(s) 30
MseI TTAA 1 cut(s) 66
MspA1I CMGCKG 1 cut(s) 141
MspI CCGG 2 cut(s) 229, 237
MspR9I CCNGG 1 cut(s) 238
MvnI CGCG 1 cut(s) 141
NciI CCSGG 1 cut(s) 238
NlaIII CATG 1 cut(s) 213
PciSI GCTCTTC 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 30
PkrI GCNGC 1 cut(s) 17
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PpuMI RGGWCCY 1 cut(s) 7
Psp1406I AACGTT 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 7
PspPI GGNCC 2 cut(s) 7, 226
PspPPI RGGWCCY 1 cut(s) 7
SacII CCGCGG 1 cut(s) 142
SapI GCTCTTC 1 cut(s) 220
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 1 cut(s) 16
Sau96I GGNCC 2 cut(s) 7, 226
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 1 cut(s) 238
SduI GDGCHC 3 cut(s) 119, 147, 155
SetI ASST 2 cut(s) 12, 99
Sfr303I CCGCGG 1 cut(s) 142
SgrBI CCGCGG 1 cut(s) 142
SinI GGWCC 1 cut(s) 7
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SpeI ACTAGT 1 cut(s) 82
SsiI CCGC 3 cut(s) 139, 141, 195
SspI AATATT 1 cut(s) 22
SspMI CTAG 1 cut(s) 83
StyD4I CCNGG 1 cut(s) 236
TaaI ACNGT 1 cut(s) 114
TaiI ACGT 1 cut(s) 99
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TseI GCWGC 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 7
XmnI GAANNNNTTC 1 cut(s) 30
XspI CTAG 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.