Rw2G024900

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017818
Physical Location & Seq
Reverse (-)
37027599 .. 37032985
5387 bp
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UTR
Exon/CDS
Intron
Rw2G024900.1

Sequence Viewer

Length: 573 bp
ATGGAGGATGTTAAGTCTTTAATGAGACTAGTCAACAACAACGTTTTAGACAATGTACCGTGCTCAGTGGCTGTCTTTGTTGACCGCGGGCTCAAGAGCACATTTATAGAGTCGTCCACCAACAACAACGCCAATAACCACCGCCACCATTACGCCATGCTCTTCGCTGGTGGGCCAGATGACCGGGAGGCATTAGCATATGCATGGAGGATGGCCGAAAACCTTAATGTTAATTTGACTGTTATGAAATTCATTGCCAGTGAAAATGCTACGTTTGATTTTACATATGAAGATGGGAACGAAATTCTAAAGGCCATGCAAGACAATGAGAGGCAGAAGCTACTAAACGACCAGTGTATAGAGAGTATCCTGATCAATACTAGGAACAACCCTTCAATAAAATTAACTGAGGAGGTGGTCTCTGCCAAGTATGTCCCTCCTATGAGCCCAATTGGCCGCGGCGCGTACGGGTTTGTTTGCTATGCAGCAGACTCTGAGACAAAAGAAGAGGTTGCAATCAAGAAGATTGGGAATGCATTTGACAATAGGATCGATGCCAAGAGGACGCTCTGA

Protein Analysis

190

Amino Acids

21.34

Weight (kDa)

5.28

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHX17_C PF23259 53 - 94 3e-08 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017818)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0028811
rosa_laevigata RLG00000018790 RLG00000034794
rosa_roxburghii Rroxscaffold_1G00051090 Rroxscaffold_2G00120030
rosa_samantha Rh1AG072100 Rh1CG071500 Rh2AG308900 Rh2AG434900 Rh2CG420900 Rh5BG200900
rosa_wichuraiana Rw2G024900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 87, 459, 464
AciI CCGC 5 cut(s) 85, 87, 142, 457, 459
AclI AACGTT 1 cut(s) 42
AclWI GGATC 1 cut(s) 557
AcoI YGGCCR 2 cut(s) 213, 454
AcsI RAATTY 2 cut(s) 248, 303
AfaI GTAC 2 cut(s) 57, 467
AgsI TTSAA 1 cut(s) 396
AhlI ACTAGT 1 cut(s) 28
AluBI AGCT 1 cut(s) 340
AluI AGCT 1 cut(s) 340
Alw21I GWGCWC 2 cut(s) 65, 101
Alw26I GTCTC 3 cut(s) 19, 424, 491
AlwI GGATC 1 cut(s) 557
AlwNI CAGNNNCTG 2 cut(s) 71, 494
AoxI GGCC 4 cut(s) 173, 213, 312, 454
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 2 cut(s) 248, 303
AspLEI GCGC 1 cut(s) 464
AspS9I GGNCC 1 cut(s) 173
AsuC2I CCSGG 1 cut(s) 185
BanII GRGCYC 2 cut(s) 93, 449
Bbv12I GWGCWC 2 cut(s) 65, 101
BbvI GCAGC 1 cut(s) 497
BccI CCATC 2 cut(s) 205, 287
BciVI GTATCC 1 cut(s) 377
BclI TGATCA 1 cut(s) 372
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 3 cut(s) 19, 424, 491
BcuI ACTAGT 1 cut(s) 28
BfaI CTAG 2 cut(s) 29, 381
BfuI GTATCC 1 cut(s) 377
BglI GCCNNNNNGGC 1 cut(s) 453
BisI GCNGC 3 cut(s) 457, 460, 486
BlsI GCNGC 3 cut(s) 458, 461, 487
Bme1390I CCNGG 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 173
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 1 cut(s) 544
BplI GAGNNNNNCTC 2 cut(s) 404, 436
BpuEI CTTGAG 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 185
Bsa29I ATCGAT 1 cut(s) 552
BsaI GGTCTC 1 cut(s) 424
BsaJI CCNNGG 2 cut(s) 85, 457
Bse1I ACTGG 2 cut(s) 258, 352
Bse3DI GCAATG 1 cut(s) 252
BseCI ATCGAT 1 cut(s) 552
BseDI CCNNGG 2 cut(s) 85, 457
BseGI GGATG 2 cut(s) 13, 216
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 3 cut(s) 78, 399, 486
BseNI ACTGG 2 cut(s) 258, 352
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 1 cut(s) 497
Bsh1236I CGCG 3 cut(s) 87, 459, 464
BshFI GGCC 4 cut(s) 175, 215, 314, 456
BshVI ATCGAT 1 cut(s) 552
BsiHKAI GWGCWC 2 cut(s) 65, 101
BsiSI CCGG 1 cut(s) 184
BsiWI CGTACG 1 cut(s) 465
BslFI GGGAC 1 cut(s) 419
BsmAI GTCTC 3 cut(s) 19, 424, 491
BsmFI GGGAC 1 cut(s) 419
BsmI GAATGC 1 cut(s) 538
BsnI GGCC 4 cut(s) 175, 215, 314, 456
Bso31I GGTCTC 1 cut(s) 424
Bsp1286I GDGCHC 4 cut(s) 65, 93, 101, 449
Bsp143I GATC 2 cut(s) 372, 549
BspACI CCGC 5 cut(s) 85, 87, 142, 457, 459
BspANI GGCC 4 cut(s) 175, 215, 314, 456
BspCNI CTCAG 3 cut(s) 77, 400, 487
BspDI ATCGAT 1 cut(s) 552
BspFNI CGCG 3 cut(s) 87, 459, 464
BspPI GGATC 1 cut(s) 557
BspQI GCTCTTC 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 252
BsrI ACTGG 2 cut(s) 258, 352
BssECI CCNNGG 2 cut(s) 85, 457
BssMI GATC 2 cut(s) 372, 549
Bst4CI ACNGT 2 cut(s) 60, 241
Bst6I CTCTTC 2 cut(s) 167, 501
BstC8I GCNNGC 1 cut(s) 89
BstDEI CTNAG 3 cut(s) 64, 408, 495
BstDSI CCRYGG 2 cut(s) 85, 457
BstF5I GGATG 2 cut(s) 13, 216
BstFNI CGCG 3 cut(s) 87, 459, 464
BstHHI GCGC 1 cut(s) 464
BstKTI GATC 2 cut(s) 375, 552
BstMAI GTCTC 3 cut(s) 19, 424, 491
BstMBI GATC 2 cut(s) 372, 549
BstMWI GCNNNNNNNGC 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 183
BstUI CGCG 3 cut(s) 87, 459, 464
BstV1I GCAGC 1 cut(s) 497
Bsu15I ATCGAT 1 cut(s) 552
BsuI GTATCC 1 cut(s) 377
BsuRI GGCC 4 cut(s) 175, 215, 314, 456
BsuTUI ATCGAT 1 cut(s) 552
BtgI CCRYGG 2 cut(s) 85, 457
BtsCI GGATG 2 cut(s) 13, 216
BtsIMutI CAGTG 3 cut(s) 72, 265, 359
Cac8I GCNNGC 1 cut(s) 89
CaiI CAGNNNCTG 2 cut(s) 71, 494
CfoI GCGC 1 cut(s) 464
Cfr13I GGNCC 1 cut(s) 173
Cfr42I CCGCGG 2 cut(s) 88, 460
ClaI ATCGAT 1 cut(s) 552
Csp6I GTAC 2 cut(s) 56, 466
CviAII CATG 3 cut(s) 157, 204, 316
CviJI RGCY 8 cut(s) 71, 91, 175, 215, 314, 340, 447, 456
CviKI_1 RGCY 8 cut(s) 71, 91, 175, 215, 314, 340, 447, 456
CviQI GTAC 2 cut(s) 56, 466
DdeI CTNAG 3 cut(s) 64, 408, 495
DpnI GATC 2 cut(s) 374, 551
DpnII GATC 2 cut(s) 372, 549
EaeI YGGCCR 2 cut(s) 213, 454
Eam1104I CTCTTC 2 cut(s) 167, 501
EarI CTCTTC 2 cut(s) 167, 501
Eco24I GRGCYC 2 cut(s) 93, 449
Eco31I GGTCTC 1 cut(s) 424
EcoT22I ATGCAT 2 cut(s) 205, 538
EcoT38I GRGCYC 2 cut(s) 93, 449
FaeI CATG 3 cut(s) 160, 207, 319
FaqI GGGAC 1 cut(s) 419
FatI CATG 3 cut(s) 156, 203, 315
FauI CCCGC 1 cut(s) 80
FauNDI CATATG 2 cut(s) 199, 286
FbaI TGATCA 1 cut(s) 372
Fnu4HI GCNGC 3 cut(s) 457, 460, 486
FokI GGATG 2 cut(s) 20, 223
FriOI GRGCYC 2 cut(s) 93, 449
Fsp4HI GCNGC 3 cut(s) 457, 460, 486
FspBI CTAG 2 cut(s) 29, 381
GlaI GCGC 1 cut(s) 463
GluI GCNGC 3 cut(s) 457, 460, 486
HaeIII GGCC 4 cut(s) 175, 215, 314, 456
HapII CCGG 1 cut(s) 184
HhaI GCGC 1 cut(s) 464
Hin1II CATG 3 cut(s) 160, 207, 319
Hin6I GCGC 1 cut(s) 462
HinP1I GCGC 1 cut(s) 462
HincII GTYRAC 2 cut(s) 34, 82
HindII GTYRAC 2 cut(s) 34, 82
HinfI GANTC 2 cut(s) 110, 491
HpaII CCGG 1 cut(s) 184
Hpy166II GTNNAC 3 cut(s) 34, 82, 117
Hpy188I TCNGA 2 cut(s) 496, 572
Hpy188III TCNNGA 3 cut(s) 94, 370, 520
Hpy8I GTNNAC 3 cut(s) 34, 82, 117
HpyAV CCTTC 1 cut(s) 402
HpyCH4III ACNGT 2 cut(s) 60, 241
HpyCH4IV ACGT 2 cut(s) 42, 272
HpyCH4V TGCA 5 cut(s) 203, 319, 485, 515, 536
HpyF10VI GCNNNNNNNGC 1 cut(s) 453
HpyF3I CTNAG 3 cut(s) 64, 408, 495
HpySE526I ACGT 2 cut(s) 42, 272
Hsp92II CATG 3 cut(s) 160, 207, 319
HspAI GCGC 1 cut(s) 462
Ksp22I TGATCA 1 cut(s) 372
KspI CCGCGG 2 cut(s) 88, 460
Kzo9I GATC 2 cut(s) 372, 549
LguI GCTCTTC 1 cut(s) 167
LpnPI CCDG 6 cut(s) 153, 189, 197, 271, 365, 383
Lsp1109I GCAGC 1 cut(s) 497
LweI GCATC 1 cut(s) 544
MaeI CTAG 2 cut(s) 29, 381
MaeII ACGT 2 cut(s) 42, 272
MalI GATC 2 cut(s) 374, 551
MboI GATC 2 cut(s) 372, 549
MboII GAAGA 4 cut(s) 154, 302, 518, 535
MfeI CAATTG 1 cut(s) 450
MhlI GDGCHC 4 cut(s) 65, 93, 101, 449
MluCI AATT 5 cut(s) 232, 248, 303, 401, 450
MlyI GAGTC 2 cut(s) 119, 485
MnlI CCTC 8 cut(s) 181, 201, 324, 403, 406, 447, 502, 555
Mph1103I ATGCAT 2 cut(s) 205, 538
MseI TTAA 5 cut(s) 12, 20, 225, 231, 404
MslI CAYNNNNRTG 1 cut(s) 202
MspA1I CMGCKG 2 cut(s) 87, 459
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 1 cut(s) 185
MunI CAATTG 1 cut(s) 450
Mva1269I GAATGC 1 cut(s) 538
MvnI CGCG 3 cut(s) 87, 459, 464
MwoI GCNNNNNNNGC 1 cut(s) 453
NciI CCSGG 1 cut(s) 185
NdeI CATATG 2 cut(s) 199, 286
NdeII GATC 2 cut(s) 372, 549
NlaIII CATG 3 cut(s) 160, 207, 319
NsiI ATGCAT 2 cut(s) 205, 538
PciSI GCTCTTC 1 cut(s) 167
PctI GAATGC 1 cut(s) 538
Pfl23II CGTACG 1 cut(s) 465
PkrI GCNGC 3 cut(s) 458, 461, 487
PleI GAGTC 2 cut(s) 118, 485
PpsI GAGTC 2 cut(s) 118, 485
Psp1406I AACGTT 1 cut(s) 42
PspLI CGTACG 1 cut(s) 465
PspPI GGNCC 1 cut(s) 173
PstNI CAGNNNCTG 2 cut(s) 71, 494
RsaI GTAC 2 cut(s) 57, 467
RsaNI GTAC 2 cut(s) 56, 466
RseI CAYNNNNRTG 1 cut(s) 202
SacII CCGCGG 2 cut(s) 88, 460
SapI GCTCTTC 1 cut(s) 167
SaqAI TTAA 5 cut(s) 12, 20, 225, 231, 404
SatI GCNGC 3 cut(s) 457, 460, 486
Sau3AI GATC 2 cut(s) 372, 549
Sau96I GGNCC 1 cut(s) 173
SchI GAGTC 2 cut(s) 119, 485
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 4 cut(s) 65, 93, 101, 449
SetI ASST 6 cut(s) 45, 225, 275, 342, 417, 513
SfaNI GCATC 1 cut(s) 544
Sfr303I CCGCGG 2 cut(s) 88, 460
SgrBI CCGCGG 2 cut(s) 88, 460
SmiMI CAYNNNNRTG 1 cut(s) 202
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
SpeI ACTAGT 1 cut(s) 28
Sse9I AATT 5 cut(s) 232, 248, 303, 401, 450
SsiI CCGC 5 cut(s) 85, 87, 142, 457, 459
SspMI CTAG 2 cut(s) 29, 381
StyD4I CCNGG 1 cut(s) 183
TaaI ACNGT 2 cut(s) 60, 241
TaiI ACGT 2 cut(s) 45, 275
TaqI TCGA 1 cut(s) 552
TasI AATT 5 cut(s) 232, 248, 303, 401, 450
TauI GCSGC 2 cut(s) 459, 462
Tru1I TTAA 5 cut(s) 12, 20, 225, 231, 404
Tru9I TTAA 5 cut(s) 12, 20, 225, 231, 404
TscAI CASTG 3 cut(s) 72, 265, 359
TseI GCWGC 1 cut(s) 485
TspDTI ATGAA 3 cut(s) 241, 260, 303
TspRI CASTG 3 cut(s) 72, 265, 359
XapI RAATTY 2 cut(s) 248, 303
XspI CTAG 2 cut(s) 29, 381
Zsp2I ATGCAT 2 cut(s) 205, 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.