Rroxscaffold_2G00122900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016968
Physical Location & Seq
Reverse (-)
56321248 .. 56323853
2606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00122900.1

Sequence Viewer

Length: 1245 bp
ATGGATCGATCGGCGAGCTCATTACGAACTCATCCAAGGAAACCCGAAGCTTTGCCGACGTCGGAGCTGCATTTTCCGGCCGGGTCGAGCTTCATCCAACCGCGCAGCCTTGCAGCGCCGTCGTGGGCACGAATCGTCGACGGCGACCACCACGAGAGACGATCGGAAGGAGGAGGCGACCCCGCCGACAAAGTTTCACGGCGGAGGTGGCCGGAGCCGCCATGCTCAGCTACTCCAAAACCTACCAAGTATCTCCGATTGTTCAAGAAGTCTATGGAAAAAGTTAATGACGGTTCTCTGAAGGCCACTGATGGAGACGACGAGGATCTTCAGCTTTTAGGGAACCTTCTGGCAGATGATGTGGAAGAGCAGAACTTGGATGACGAGTTTTTCACTCCTAAGTCTACACGTCCATTTGGAGATGCTCCTGCTTACGATGACTCCGTACGCGTGGTAGTTGCATCACATGTTTCTCAAGAGATTGTAGGTTGGATTTGGCTTGTTTTCCACGGTATTGTTGAGTGTATTCTTGGCTTTGTGGAGATTTATGGGATAGGGAATCCCACGTGCATGCTCACAGGTGTTGTTTCATCAGCCGTAGCAGCATACTTTTGGATTGCCGAGATTGTGGGGATGCACCGTGTACCGTTCAGCTTTTTGAGACTACCATTTATTCCAGAGATTTACAGACTGGTGGTAGTTTTTGTGCAGTTCACATACTTTCTGATGGGATATGTGTCATTGAGACATGATTCAGAGGAGACAGTTAGAGGACCTATTATTCGTATATGCGTTTATAGCTTGTCTTGTCAGATTTGCTTATTTCTACAAGCGACCTCGGGGCTTGAGCATAGCGAGGCGGCGGAAGTTTCTTCGGTTACCATCTACTTTAGCACCGATGAAGCTTTTTGTCGGGGGTCGGAGACATCACGGGGTACCGGATCCCCGAGTGAAGAGGCGGAGCAACTTTTGGTTGGTGGCTATCGTCGGGTTTGTGTGGTATCGACACTAGAGTCTCGGCCGGTGTTCATACGGACAAGGCGAGAGTCATCGAGGTCGAGGTGGTCCGGGTTTGACGTCCGGCCACCTAGTATTGACCCCGAAGCTTTCAATCGGTGGTGGAGGCTGCGGCGTATAGAGTTACGGCGTTTTGCGAGTTTGACGGTCGCCGGTGGGTCTAATCGTAGTTATGATGCGGTGGTGGCTAGGGCGGCCGCCGGAGGAAGCCCGGCGGTCGTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

414

Amino Acids

46.07

Weight (kDa)

6.18

Isoelectric Point (pI)

63.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1012
AatII GACGTC 2 cut(s) 62, 1080
Acc65I GGTACC 1 cut(s) 935
AccB1I GGYRCC 1 cut(s) 935
AccI GTMKAC 2 cut(s) 138, 404
AccII CGCG 2 cut(s) 103, 450
AclWI GGATC 4 cut(s) 12, 333, 936, 949
AcoI YGGCCR 5 cut(s) 78, 209, 1019, 1082, 1212
AcuI CTGAAG 2 cut(s) 314, 320
AcvI CACGTG 1 cut(s) 567
AcyI GRCGYC 2 cut(s) 59, 1077
AfaI GTAC 3 cut(s) 447, 645, 937
AflIII ACRYGT 3 cut(s) 407, 448, 466
AgsI TTSAA 2 cut(s) 265, 1111
AjiI CACGTC 1 cut(s) 410
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 7 cut(s) 151, 309, 655, 739, 755, 917, 1020
AlwI GGATC 4 cut(s) 12, 333, 936, 949
Ama87I CYCGRG 2 cut(s) 838, 946
AoxI GGCC 7 cut(s) 78, 209, 303, 1019, 1082, 1212, 1239
ApeKI GCWGC 5 cut(s) 67, 105, 113, 602, 1126
ArsI GACNNNNNNTTYG 2 cut(s) 765, 797
Asp718I GGTACC 1 cut(s) 935
AspLEI GCGC 2 cut(s) 105, 118
AspS9I GGNCC 2 cut(s) 773, 1065
AsuC2I CCSGG 3 cut(s) 82, 1069, 1229
AvaI CYCGRG 2 cut(s) 838, 946
AvaII GGWCC 2 cut(s) 773, 1065
BaeGI GKGCMC 1 cut(s) 130
BamHI GGATCC 1 cut(s) 941
BanI GGYRCC 1 cut(s) 935
BanII GRGCYC 1 cut(s) 20
BauI CACGAG 1 cut(s) 152
BbrPI CACGTG 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 5 cut(s) 54, 117, 125, 614, 1113
BccI CCATC 3 cut(s) 305, 721, 890
BceAI ACGGC 5 cut(s) 103, 157, 215, 581, 1160
BcgI CGANNNNNNTGC 2 cut(s) 102, 136
BcnI CCSGG 3 cut(s) 82, 1069, 1229
BcoDI GTCTC 7 cut(s) 151, 309, 655, 739, 755, 917, 1020
BfaI CTAG 4 cut(s) 1010, 1089, 1206, 1243
BfoI RGCGCY 1 cut(s) 119
BlpI GCTNAGC 1 cut(s) 226
Bme1390I CCNGG 3 cut(s) 82, 1069, 1229
Bme18I GGWCC 2 cut(s) 773, 1065
BmeT110I CYCGRG 2 cut(s) 838, 946
BmgBI CACGTC 1 cut(s) 410
BmgT120I GGNCC 2 cut(s) 773, 1065
BmiI GGNNCC 4 cut(s) 216, 344, 937, 943
BmrFI CCNGG 3 cut(s) 82, 1069, 1229
BmsI GCATC 4 cut(s) 412, 470, 624, 1183
Bpu1102I GCTNAGC 1 cut(s) 226
BpuEI CTTGAG 2 cut(s) 459, 866
BpuMI CCSGG 3 cut(s) 82, 1069, 1229
Bsa29I ATCGAT 1 cut(s) 7
BsaAI YACGTR 1 cut(s) 567
BsaHI GRCGYC 2 cut(s) 59, 1077
BsaJI CCNNGG 3 cut(s) 35, 508, 837
BsaWI WCCGGW 1 cut(s) 938
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bse118I RCCGGY 2 cut(s) 1021, 1169
Bse1I ACTGG 1 cut(s) 696
BseCI ATCGAT 1 cut(s) 7
BseDI CCNNGG 3 cut(s) 35, 508, 837
BseGI GGATG 4 cut(s) 31, 93, 385, 639
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 1 cut(s) 696
BseRI GAGGAG 2 cut(s) 186, 773
BseSI GKGCMC 1 cut(s) 130
BseX3I CGGCCG 3 cut(s) 78, 1019, 1212
BseXI GCAGC 5 cut(s) 54, 117, 125, 614, 1113
BsgI GTGCAG 1 cut(s) 728
Bsh1236I CGCG 2 cut(s) 103, 450
Bsh1285I CGRYCG 7 cut(s) 11, 81, 164, 1022, 1167, 1215, 1236
BshFI GGCC 7 cut(s) 80, 211, 305, 1021, 1084, 1214, 1241
BshNI GGYRCC 1 cut(s) 935
BshVI ATCGAT 1 cut(s) 7
BsiEI CGRYCG 7 cut(s) 11, 81, 164, 1022, 1167, 1215, 1236
BsiHKAI GWGCWC 1 cut(s) 20
BsiHKCI CYCGRG 2 cut(s) 838, 946
BsiWI CGTACG 1 cut(s) 445
BsmAI GTCTC 7 cut(s) 151, 309, 655, 739, 755, 917, 1020
BsmBI CGTCTC 2 cut(s) 151, 309
BsnI GGCC 7 cut(s) 80, 211, 305, 1021, 1084, 1214, 1241
BsoBI CYCGRG 2 cut(s) 838, 946
Bsp1286I GDGCHC 2 cut(s) 20, 130
Bsp143I GATC 5 cut(s) 4, 8, 161, 325, 941
Bsp1720I GCTNAGC 1 cut(s) 226
BspANI GGCC 7 cut(s) 80, 211, 305, 1021, 1084, 1214, 1241
BspCNI CTCAG 1 cut(s) 239
BspDI ATCGAT 1 cut(s) 7
BspFNI CGCG 2 cut(s) 103, 450
BspLI GGNNCC 4 cut(s) 216, 344, 937, 943
BspPI GGATC 4 cut(s) 12, 333, 936, 949
BspQI GCTCTTC 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 935
BsrFI RCCGGY 2 cut(s) 1021, 1169
BsrI ACTGG 1 cut(s) 696
BssAI RCCGGY 2 cut(s) 1021, 1169
BssECI CCNNGG 3 cut(s) 35, 508, 837
BssMI GATC 5 cut(s) 4, 8, 161, 325, 941
BssNI GRCGYC 2 cut(s) 59, 1077
BssSI CACGAG 1 cut(s) 152
BssT1I CCWWGG 1 cut(s) 35
Bst2BI CACGAG 1 cut(s) 152
Bst4CI ACNGT 6 cut(s) 293, 512, 641, 648, 766, 1165
Bst6I CTCTTC 2 cut(s) 360, 948
BstACI GRCGYC 2 cut(s) 59, 1077
BstBAI YACGTR 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 16, 572
BstDEI CTNAG 2 cut(s) 226, 399
BstDSI CCRYGG 1 cut(s) 508
BstEII GGTNACC 1 cut(s) 877
BstF5I GGATG 4 cut(s) 31, 93, 385, 639
BstFNI CGCG 2 cut(s) 103, 450
BstH2I RGCGCY 1 cut(s) 119
BstHHI GCGC 2 cut(s) 105, 118
BstKTI GATC 5 cut(s) 7, 11, 164, 328, 944
BstMAI GTCTC 7 cut(s) 151, 309, 655, 739, 755, 917, 1020
BstMBI GATC 5 cut(s) 4, 8, 161, 325, 941
BstMCI CGRYCG 7 cut(s) 11, 81, 164, 1022, 1167, 1215, 1236
BstMWI GCNNNNNNNGC 7 cut(s) 208, 217, 602, 798, 1202, 1211, 1238
BstNSI RCATGY 2 cut(s) 470, 574
BstPI GGTNACC 1 cut(s) 877
BstSCI CCNGG 3 cut(s) 80, 1067, 1227
BstSLI GKGCMC 1 cut(s) 130
BstUI CGCG 2 cut(s) 103, 450
BstV1I GCAGC 5 cut(s) 54, 117, 125, 614, 1113
BstX2I RGATCY 2 cut(s) 325, 941
BstYI RGATCY 2 cut(s) 325, 941
BstZI CGGCCG 3 cut(s) 78, 1019, 1212
Bsu15I ATCGAT 1 cut(s) 7
BsuRI GGCC 7 cut(s) 80, 211, 305, 1021, 1084, 1214, 1241
BsuTUI ATCGAT 1 cut(s) 7
BtgI CCRYGG 1 cut(s) 508
BtrI CACGTC 1 cut(s) 410
BtsCI GGATG 4 cut(s) 31, 93, 385, 639
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 2 cut(s) 16, 572
CciNI GCGGCCGC 1 cut(s) 1212
CfoI GCGC 2 cut(s) 105, 118
Cfr10I RCCGGY 2 cut(s) 1021, 1169
Cfr13I GGNCC 2 cut(s) 773, 1065
ClaI ATCGAT 1 cut(s) 7
Csp6I GTAC 3 cut(s) 446, 644, 936
CviAII CATG 4 cut(s) 222, 467, 571, 749
CviQI GTAC 3 cut(s) 446, 644, 936
DdeI CTNAG 2 cut(s) 226, 399
DpnI GATC 5 cut(s) 6, 10, 163, 327, 943
DpnII GATC 5 cut(s) 4, 8, 161, 325, 941
DrdI GACNNNNNNGTC 1 cut(s) 1012
DseDI GACNNNNNNGTC 1 cut(s) 1012
EaeI YGGCCR 5 cut(s) 78, 209, 1019, 1082, 1212
EagI CGGCCG 3 cut(s) 78, 1019, 1212
Eam1104I CTCTTC 2 cut(s) 360, 948
EarI CTCTTC 2 cut(s) 360, 948
EciI GGCGGA 3 cut(s) 217, 878, 974
Ecl136II GAGCTC 1 cut(s) 18
EclXI CGGCCG 3 cut(s) 78, 1019, 1212
Eco130I CCWWGG 1 cut(s) 35
Eco24I GRGCYC 1 cut(s) 20
Eco47I GGWCC 2 cut(s) 773, 1065
Eco52I CGGCCG 3 cut(s) 78, 1019, 1212
Eco53kI GAGCTC 1 cut(s) 18
Eco57I CTGAAG 2 cut(s) 314, 320
Eco72I CACGTG 1 cut(s) 567
Eco88I CYCGRG 2 cut(s) 838, 946
Eco91I GGTNACC 1 cut(s) 877
EcoICRI GAGCTC 1 cut(s) 18
EcoO109I RGGNCCY 1 cut(s) 773
EcoO65I GGTNACC 1 cut(s) 877
EcoT14I CCWWGG 1 cut(s) 35
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 35
Esp3I CGTCTC 2 cut(s) 151, 309
FaeI CATG 4 cut(s) 225, 470, 574, 752
FatI CATG 4 cut(s) 221, 466, 570, 748
FauI CCCGC 1 cut(s) 190
FblI GTMKAC 2 cut(s) 138, 404
FokI GGATG 4 cut(s) 18, 80, 392, 646
FriOI GRGCYC 1 cut(s) 20
FspBI CTAG 4 cut(s) 1010, 1089, 1206, 1243
GlaI GCGC 2 cut(s) 104, 117
HaeII RGCGCY 1 cut(s) 119
HaeIII GGCC 7 cut(s) 80, 211, 305, 1021, 1084, 1214, 1241
HhaI GCGC 2 cut(s) 105, 118
Hin1I GRCGYC 2 cut(s) 59, 1077
Hin1II CATG 4 cut(s) 225, 470, 574, 752
Hin6I GCGC 2 cut(s) 103, 116
HinP1I GCGC 2 cut(s) 103, 116
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 3 cut(s) 48, 903, 1104
HinfI GANTC 6 cut(s) 132, 440, 559, 752, 1013, 1046
Hpy166II GTNNAC 4 cut(s) 139, 405, 644, 714
Hpy188I TCNGA 8 cut(s) 64, 166, 257, 300, 726, 757, 813, 922
Hpy188III TCNNGA 3 cut(s) 265, 476, 677
Hpy8I GTNNAC 4 cut(s) 139, 405, 644, 714
Hpy99I CGWCG 7 cut(s) 61, 64, 124, 140, 143, 323, 990
HpyAV CCTTC 3 cut(s) 161, 295, 356
HpyCH4III ACNGT 6 cut(s) 293, 512, 641, 648, 766, 1165
HpyCH4IV ACGT 4 cut(s) 59, 409, 566, 1077
HpyCH4V TGCA 6 cut(s) 70, 113, 461, 570, 637, 709
HpyF10VI GCNNNNNNNGC 7 cut(s) 208, 217, 602, 798, 1202, 1211, 1238
HpyF3I CTNAG 2 cut(s) 226, 399
HpySE526I ACGT 4 cut(s) 59, 409, 566, 1077
Hsp92I GRCGYC 2 cut(s) 59, 1077
Hsp92II CATG 4 cut(s) 225, 470, 574, 752
HspAI GCGC 2 cut(s) 103, 116
KpnI GGTACC 1 cut(s) 939
Kzo9I GATC 5 cut(s) 4, 8, 161, 325, 941
LguI GCTCTTC 1 cut(s) 360
LmnI GCTCC 4 cut(s) 64, 214, 430, 961
Lsp1109I GCAGC 5 cut(s) 54, 117, 125, 614, 1113
LweI GCATC 4 cut(s) 412, 470, 624, 1183
MaeI CTAG 4 cut(s) 1010, 1089, 1206, 1243
MaeII ACGT 4 cut(s) 59, 409, 566, 1077
MaeIII GTNAC 2 cut(s) 877, 1140
MalI GATC 5 cut(s) 6, 10, 163, 327, 943
MboI GATC 5 cut(s) 4, 8, 161, 325, 941
MboII GAAGA 4 cut(s) 320, 377, 864, 965
MflI RGATCY 2 cut(s) 325, 941
MhlI GDGCHC 2 cut(s) 20, 130
MluI ACGCGT 1 cut(s) 448
MlyI GAGTC 3 cut(s) 434, 1022, 1055
MmeI TCCRAC 4 cut(s) 42, 121, 470, 900
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 1 cut(s) 569
MspR9I CCNGG 3 cut(s) 82, 1069, 1229
MvnI CGCG 2 cut(s) 103, 450
MwoI GCNNNNNNNGC 7 cut(s) 208, 217, 602, 798, 1202, 1211, 1238
NciI CCSGG 3 cut(s) 82, 1069, 1229
NdeII GATC 5 cut(s) 4, 8, 161, 325, 941
NlaIII CATG 4 cut(s) 225, 470, 574, 752
NlaIV GGNNCC 4 cut(s) 216, 344, 937, 943
NmeAIII GCCGAG 2 cut(s) 646, 997
NotI GCGGCCGC 1 cut(s) 1212
NspI RCATGY 2 cut(s) 470, 574
PaeI GCATGC 1 cut(s) 574
PciI ACATGT 1 cut(s) 466
PciSI GCTCTTC 1 cut(s) 360
PcsI WCGNNNNNNNCGW 3 cut(s) 141, 441, 1039
PfeI GAWTC 3 cut(s) 132, 559, 752
Pfl23II CGTACG 1 cut(s) 445
Ple19I CGATCG 2 cut(s) 11, 164
PleI GAGTC 3 cut(s) 434, 1021, 1054
PmaCI CACGTG 1 cut(s) 567
PmlI CACGTG 1 cut(s) 567
PpsI GAGTC 3 cut(s) 434, 1021, 1054
Ppu21I YACGTR 1 cut(s) 567
PpuMI RGGWCCY 1 cut(s) 773
PscI ACATGT 1 cut(s) 466
Psp124BI GAGCTC 1 cut(s) 20
Psp5II RGGWCCY 1 cut(s) 773
PspCI CACGTG 1 cut(s) 567
PspEI GGTNACC 1 cut(s) 877
PspLI CGTACG 1 cut(s) 445
PspN4I GGNNCC 4 cut(s) 216, 344, 937, 943
PspPI GGNCC 2 cut(s) 773, 1065
PspPPI RGGWCCY 1 cut(s) 773
PsuI RGATCY 2 cut(s) 325, 941
PvuI CGATCG 2 cut(s) 11, 164
RsaI GTAC 3 cut(s) 447, 645, 937
RsaNI GTAC 3 cut(s) 446, 644, 936
RseI CAYNNNNRTG 1 cut(s) 569
SacI GAGCTC 1 cut(s) 20
SalI GTCGAC 1 cut(s) 137
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 1 cut(s) 285
Sau3AI GATC 5 cut(s) 4, 8, 161, 325, 941
Sau96I GGNCC 2 cut(s) 773, 1065
SchI GAGTC 3 cut(s) 434, 1022, 1055
ScrFI CCNGG 3 cut(s) 82, 1069, 1229
SduI GDGCHC 2 cut(s) 20, 130
SfaNI GCATC 4 cut(s) 412, 470, 624, 1183
SgrAI CRCCGGYG 1 cut(s) 1169
SgrDI CGTCGACG 1 cut(s) 137
SinI GGWCC 2 cut(s) 773, 1065
SmiMI CAYNNNNRTG 1 cut(s) 569
SmlI CTYRAG 2 cut(s) 474, 845
SmoI CTYRAG 2 cut(s) 474, 845
SphI GCATGC 1 cut(s) 574
SspMI CTAG 4 cut(s) 1010, 1089, 1206, 1243
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 3 cut(s) 80, 1067, 1227
StyI CCWWGG 1 cut(s) 35
TaaI ACNGT 6 cut(s) 293, 512, 641, 648, 766, 1165
TaiI ACGT 4 cut(s) 62, 412, 569, 1080
TaqI TCGA 6 cut(s) 7, 86, 138, 1004, 1052, 1058
TauI GCSGC 5 cut(s) 220, 863, 1132, 1214, 1217
TfiI GAWTC 3 cut(s) 132, 559, 752
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 1 cut(s) 313
TseI GCWGC 5 cut(s) 67, 105, 113, 602, 1126
TspDTI ATGAA 4 cut(s) 82, 579, 915, 1018
TspGWI ACGGA 2 cut(s) 433, 1048
TspRI CASTG 1 cut(s) 313
VpaK11BI GGWCC 2 cut(s) 773, 1065
XceI RCATGY 2 cut(s) 470, 574
XmiI GTMKAC 2 cut(s) 138, 404
XspI CTAG 4 cut(s) 1010, 1089, 1206, 1243
ZraI GACGTC 2 cut(s) 60, 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.