Rh2BG269500

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0016968
Physical Location & Seq
Reverse (-)
29009557 .. 29010165
609 bp
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UTR
Exon/CDS
Intron
Rh2BG269500.1

Sequence Viewer

Length: 609 bp
ATGCTTGCTGAGGAGGCTGATGACCTTGTTGAGCAGCAAAGGGCCAATACCCAACTAGGTCGTACATACCATGAGATGTTTAGAAAATCCCTGGGGAAACATCAGTCAATGCAGAATGCGAAGAAGTATTTCATGTCCGCTATGAAGCTTGCACAGACTATCAAGGATAATCCACCTACTAATAAGTCTTTCTTCATCAAAGAATATATTGACGCATATAGCAATATAGGATTGCTTGAACTTGATCTTGACAACCTGGAAGAGGCTGAGAAAATCTTTGCTAAAGCATTAGATATCTGCAACGAAGAAGAGGTAAATGAATACGATGAGGCCCGCGCCAGGCTCCATCACAACCTTGGAAAGGTTTACACTGAGCTGAGAATCTGGGATAATGCTTGGCAACACTTTGACAGAGACATTATGATATGTAAGCAGATTGGACATTGCAAAGGAGAGGCAAATTGGTATATCAGTCTTGGCCAGTTACTAACCGTGCACAAAAATATGAGGAGGCACTTCTTTGCTACCAGAAAGCACTTGATCTGGCGAAATCAATGGAAGATGAGGATGCCTTGGTCCATCAAATTGATGGGAGTATCAAAACTGTAA

Protein Analysis

202

Amino Acids

24.04

Weight (kDa)

8.66

Isoelectric Point (pI)

31.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 71 - 126 2.8e-09 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 336
AciI CCGC 2 cut(s) 138, 334
AcoI YGGCCR 1 cut(s) 478
AfaI GTAC 1 cut(s) 64
AfiI CCNNNNNNNGG 3 cut(s) 262, 339, 361
AgsI TTSAA 1 cut(s) 239
AjnI CCWGG 3 cut(s) 90, 255, 338
AluBI AGCT 2 cut(s) 148, 376
AluI AGCT 2 cut(s) 148, 376
Alw21I GWGCWC 1 cut(s) 498
Alw26I GTCTC 1 cut(s) 408
Alw44I GTGCAC 1 cut(s) 494
AoxI GGCC 3 cut(s) 42, 330, 478
ApaLI GTGCAC 1 cut(s) 494
ApeKI GCWGC 1 cut(s) 34
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 1 cut(s) 338
AspS9I GGNCC 3 cut(s) 42, 331, 576
AvaII GGWCC 1 cut(s) 576
BaeGI GKGCMC 1 cut(s) 498
BalI TGGCCA 1 cut(s) 480
Bbv12I GWGCWC 1 cut(s) 498
BbvCI CCTCAGC 1 cut(s) 9
BbvI GCAGC 1 cut(s) 46
BccI CCATC 3 cut(s) 354, 583, 587
BciT130I CCWGG 3 cut(s) 92, 257, 340
BcoDI GTCTC 1 cut(s) 408
BfaI CTAG 1 cut(s) 56
BisI GCNGC 1 cut(s) 35
BlsI GCNGC 1 cut(s) 36
Bme1390I CCNGG 3 cut(s) 92, 257, 340
Bme18I GGWCC 1 cut(s) 576
BmgT120I GGNCC 3 cut(s) 42, 331, 576
BmiI GGNNCC 1 cut(s) 344
BmrFI CCNGG 3 cut(s) 92, 257, 340
BmsI GCATC 1 cut(s) 558
Bpu10I CCTNAGC 1 cut(s) 9
BsaJI CCNNGG 4 cut(s) 90, 91, 355, 572
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 339, 361
Bse1I ACTGG 1 cut(s) 481
Bse3DI GCAATG 1 cut(s) 442
BseBI CCWGG 3 cut(s) 92, 257, 340
BseDI CCNNGG 4 cut(s) 90, 91, 355, 572
BseGI GGATG 1 cut(s) 573
BseLI CCNNNNNNNGG 3 cut(s) 262, 339, 361
BseMI GCAATG 1 cut(s) 442
BseMII CTCAG 3 cut(s) 258, 363, 368
BseNI ACTGG 1 cut(s) 481
BseRI GAGGAG 2 cut(s) 26, 523
BseSI GKGCMC 1 cut(s) 498
BseXI GCAGC 1 cut(s) 46
Bsh1236I CGCG 1 cut(s) 336
BshFI GGCC 3 cut(s) 44, 332, 480
BsiHKAI GWGCWC 1 cut(s) 498
BslI CCNNNNNNNGG 3 cut(s) 262, 339, 361
BsmAI GTCTC 1 cut(s) 408
BsmI GAATGC 1 cut(s) 121
BsnI GGCC 3 cut(s) 44, 332, 480
Bsp1286I GDGCHC 1 cut(s) 498
Bsp143I GATC 2 cut(s) 244, 540
BspACI CCGC 2 cut(s) 138, 334
BspANI GGCC 3 cut(s) 44, 332, 480
BspCNI CTCAG 3 cut(s) 259, 364, 369
BspFNI CGCG 1 cut(s) 336
BspLI GGNNCC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 442
BsrI ACTGG 1 cut(s) 481
BssECI CCNNGG 4 cut(s) 90, 91, 355, 572
BssMI GATC 2 cut(s) 244, 540
BssT1I CCWWGG 2 cut(s) 355, 572
Bst2UI CCWGG 3 cut(s) 92, 257, 340
Bst4CI ACNGT 2 cut(s) 493, 606
Bst6I CTCTTC 2 cut(s) 255, 303
BstC8I GCNNGC 3 cut(s) 6, 150, 334
BstDEI CTNAG 4 cut(s) 9, 267, 372, 377
BstENI CCTNNNNNAGG 2 cut(s) 260, 359
BstF5I GGATG 1 cut(s) 573
BstFNI CGCG 1 cut(s) 336
BstHHI GCGC 1 cut(s) 338
BstKTI GATC 2 cut(s) 247, 543
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 2 cut(s) 244, 540
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNI CCWGG 3 cut(s) 92, 257, 340
BstSCI CCNGG 3 cut(s) 90, 255, 338
BstSLI GKGCMC 1 cut(s) 498
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 46
BsuRI GGCC 3 cut(s) 44, 332, 480
BtsCI GGATG 1 cut(s) 573
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 3 cut(s) 6, 150, 334
CfoI GCGC 1 cut(s) 338
Cfr13I GGNCC 3 cut(s) 42, 331, 576
CseI GACGC 1 cut(s) 221
Csp6I GTAC 1 cut(s) 63
CviAII CATG 2 cut(s) 71, 133
CviJI RGCY 8 cut(s) 17, 44, 148, 266, 332, 343, 376, 480
CviKI_1 RGCY 8 cut(s) 17, 44, 148, 266, 332, 343, 376, 480
CviQI GTAC 1 cut(s) 63
DdeI CTNAG 4 cut(s) 9, 267, 372, 377
DpnI GATC 2 cut(s) 246, 542
DpnII GATC 2 cut(s) 244, 540
EaeI YGGCCR 1 cut(s) 478
Eam1104I CTCTTC 2 cut(s) 255, 303
EarI CTCTTC 2 cut(s) 255, 303
Eco130I CCWWGG 2 cut(s) 355, 572
Eco32I GATATC 1 cut(s) 295
Eco47I GGWCC 1 cut(s) 576
EcoNI CCTNNNNNAGG 2 cut(s) 260, 359
EcoRII CCWGG 3 cut(s) 90, 255, 338
EcoRV GATATC 1 cut(s) 295
EcoT14I CCWWGG 2 cut(s) 355, 572
ErhI CCWWGG 2 cut(s) 355, 572
FaeI CATG 2 cut(s) 74, 136
FalI AAGNNNNNCTT 2 cut(s) 176, 208
FatI CATG 2 cut(s) 70, 132
FauI CCCGC 1 cut(s) 341
Fnu4HI GCNGC 1 cut(s) 35
FokI GGATG 1 cut(s) 580
Fsp4HI GCNGC 1 cut(s) 35
FspBI CTAG 1 cut(s) 56
GlaI GCGC 1 cut(s) 337
GluI GCNGC 1 cut(s) 35
HaeIII GGCC 3 cut(s) 44, 332, 480
HgaI GACGC 1 cut(s) 221
HhaI GCGC 1 cut(s) 338
Hin1II CATG 2 cut(s) 74, 136
Hin6I GCGC 1 cut(s) 336
HinP1I GCGC 1 cut(s) 336
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 1 cut(s) 381
Hpy166II GTNNAC 2 cut(s) 367, 496
Hpy188III TCNNGA 1 cut(s) 248
Hpy8I GTNNAC 2 cut(s) 367, 496
HpyCH4III ACNGT 2 cut(s) 493, 606
HpyCH4V TGCA 5 cut(s) 112, 152, 300, 447, 496
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpyF3I CTNAG 4 cut(s) 9, 267, 372, 377
Hsp92II CATG 2 cut(s) 74, 136
HspAI GCGC 1 cut(s) 336
Kzo9I GATC 2 cut(s) 244, 540
LmnI GCTCC 1 cut(s) 348
Lsp1109I GCAGC 1 cut(s) 46
LweI GCATC 1 cut(s) 558
MaeI CTAG 1 cut(s) 56
MaeIII GTNAC 1 cut(s) 483
MalI GATC 2 cut(s) 246, 542
MboI GATC 2 cut(s) 244, 540
MboII GAAGA 6 cut(s) 133, 184, 272, 317, 320, 571
MhlI GDGCHC 1 cut(s) 498
MlsI TGGCCA 1 cut(s) 480
MluCI AATT 2 cut(s) 460, 584
MluNI TGGCCA 1 cut(s) 480
MnlI CCTC 9 cut(s) 4, 7, 256, 304, 322, 448, 501, 504, 558
Mox20I TGGCCA 1 cut(s) 480
MroXI GAANNNNTTC 1 cut(s) 128
MscI TGGCCA 1 cut(s) 480
Msp20I TGGCCA 1 cut(s) 480
MspR9I CCNGG 3 cut(s) 92, 257, 340
Mva1269I GAATGC 1 cut(s) 121
MvaI CCWGG 3 cut(s) 92, 257, 340
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 1 cut(s) 14
NdeII GATC 2 cut(s) 244, 540
NlaIII CATG 2 cut(s) 74, 136
NlaIV GGNNCC 1 cut(s) 344
PasI CCCWGGG 1 cut(s) 91
PctI GAATGC 1 cut(s) 121
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 381
PkrI GCNGC 1 cut(s) 36
Psp6I CCWGG 3 cut(s) 90, 255, 338
PspGI CCWGG 3 cut(s) 90, 255, 338
PspN4I GGNNCC 1 cut(s) 344
PspPI GGNCC 3 cut(s) 42, 331, 576
RsaI GTAC 1 cut(s) 64
RsaNI GTAC 1 cut(s) 63
SatI GCNGC 1 cut(s) 35
Sau3AI GATC 2 cut(s) 244, 540
Sau96I GGNCC 3 cut(s) 42, 331, 576
ScrFI CCNGG 3 cut(s) 92, 257, 340
SduI GDGCHC 1 cut(s) 498
SetI ASST 9 cut(s) 27, 61, 150, 178, 258, 315, 357, 366, 378
SfaNI GCATC 1 cut(s) 558
SinI GGWCC 1 cut(s) 576
Sse9I AATT 2 cut(s) 460, 584
SsiI CCGC 2 cut(s) 138, 334
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 3 cut(s) 90, 255, 338
StyI CCWWGG 2 cut(s) 355, 572
TaaI ACNGT 2 cut(s) 493, 606
TasI AATT 2 cut(s) 460, 584
TfiI GAWTC 1 cut(s) 381
TscAI CASTG 1 cut(s) 376
TseI GCWGC 1 cut(s) 34
TspDTI ATGAA 4 cut(s) 121, 158, 184, 333
TspRI CASTG 1 cut(s) 376
VneI GTGCAC 1 cut(s) 494
VpaK11BI GGWCC 1 cut(s) 576
XagI CCTNNNNNAGG 2 cut(s) 260, 359
XcmI CCANNNNNNNNNTGG 2 cut(s) 353, 586
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.