Rroxscaffold_2G00127640

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016968
Physical Location & Seq
Forward (+)
63251856 .. 63255122
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00127640.1

Sequence Viewer

Length: 1212 bp
ATGGCTCCGGCGGAAGCAAAACTGGTCGAAGCGGTGAGCTCGCACAGGAGCTCGAAGGCATCCGGGAGGCATGAGGAGGAGGCTCTTGAGATCCAGAAAAAACATTTAATGCTTGCTGAGGAGGCTGATGACCTTGTTGAGCAGCAAAGGGCCAATACCCAACTAGGTCGTACATACCATGAGATGTTTAGAAAATCCCTGGGGAAACATCAGTCAATGCAGAATGCGAAGAAGTATTTCATGTCCGCTATGAAGCTTGCACAGACTATCAAGGATAATCCACCTACTAATAAGTCTTCCTTTATTAAAGAATATATTGATGCATATAGCAATATAGGATTGCTTGAATTTGATCTTGACAACCTGGAAGAGGCTTTAGATATCTGCAACGAAGAAGAGGTAAATGAATACGATGAGGCACACGCTAGGCTCCATCACAATCTCGGAAAGGTTTACACTGAGATGGGAATCTGGGATAATGCTCGGCAACACATTGACAGAGACATTATGATATGTAAGCAGATTGGGAATTGCAAAGGAGAGGCTAATGGGTATATCAGTCTTGGCCAGTTACTAAACCGTGCACAGAAATATCAGGAGGCACTTCTTTGCTACCAGAAAGCACTTGATCTGGCAAAATCAATGGAAGATGAGGATGCCTTGGTCCATCAAATTGATGGGAGTATCAAAACTGTAAAAAGATTAGCTGAAGTAATGGATGACATGAAGAGAGAAGAGCAGAATCTCTACAAGCTAACAAGAGACATGGCCATAGCAAGAGGCACATCACATGAGCAGCTGCAGCAAATTGCTTCTCTTGATCGTCCTATTGACAGATCAAAGACTATCTCTGCCCGGTCAAAGACTTGGTTTGGTTGGTTGAAGTGGCCTTACAGGTCAAATGATGACAGTCCAGCTACTCCAAAACCTGCCAAGTATCTCCGATTGTTCAGGAAATCTACGGAAAAAGTTAATGACGGTTCTCTGAAGGCCATTGATGGAGATGACGAGGAGCTTCAGCTTTTGGGGAACCTTCTGGCAGATGATGTGGAAGAGCAGTCAGATGACGAGTTTTTCGCTCCCGAGTCTACACGTCCATCTGGAGATGCTCCTGCTTATGATGACTCTGTACGTGTAGTAGTTGCATCACATGTTTCTCAGGAGATTGTAGGCTGGATCTGGCTTGTTTTCGGTTTTCCACATTATTGTTGA

Protein Analysis

403

Amino Acids

45.98

Weight (kDa)

5.24

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 142 - 214 1.6e-09 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 937
AccI GTMKAC 1 cut(s) 1088
AciI CCGC 3 cut(s) 11, 32, 246
AclWI GGATC 2 cut(s) 85, 1184
AcoI YGGCCR 2 cut(s) 565, 768
AcsI RAATTY 1 cut(s) 347
AcuI CTGAAG 3 cut(s) 729, 1001, 1007
AfaI GTAC 2 cut(s) 172, 1131
AfiI CCNNNNNNNGG 1 cut(s) 370
AflIII ACRYGT 3 cut(s) 1091, 1132, 1150
AgsI TTSAA 2 cut(s) 347, 883
AjiI CACGTC 1 cut(s) 1094
AjnI CCWGG 2 cut(s) 198, 363
AluBI AGCT 9 cut(s) 39, 51, 256, 707, 754, 799, 917, 1015, 1021
AluI AGCT 9 cut(s) 39, 51, 256, 707, 754, 799, 917, 1015, 1021
Alw21I GWGCWC 3 cut(s) 41, 53, 586
Alw26I GTCTC 2 cut(s) 495, 756
Alw44I GTGCAC 1 cut(s) 582
AlwI GGATC 2 cut(s) 85, 1184
Ama87I CYCGRG 1 cut(s) 1082
AoxI GGCC 5 cut(s) 150, 565, 768, 887, 990
ApaLI GTGCAC 1 cut(s) 582
ApeKI GCWGC 4 cut(s) 142, 796, 799, 802
ApoI RAATTY 1 cut(s) 347
ArsI GACNNNNNNTTYG 2 cut(s) 1058, 1090
Asp700I GAANNNNTTC 1 cut(s) 236
AspS9I GGNCC 2 cut(s) 150, 664
AsuC2I CCSGG 2 cut(s) 64, 856
AsuHPI GGTGA 1 cut(s) 46
AvaI CYCGRG 1 cut(s) 1082
AvaII GGWCC 1 cut(s) 664
BaeGI GKGCMC 1 cut(s) 586
BalI TGGCCA 2 cut(s) 567, 770
BanII GRGCYC 2 cut(s) 41, 53
BarI GAAGNNNNNNTAC 2 cut(s) 875, 907
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 3 cut(s) 41, 53, 586
BbvCI CCTCAGC 1 cut(s) 117
BbvI GCAGC 4 cut(s) 154, 786, 808, 814
BccI CCATC 6 cut(s) 441, 457, 671, 675, 992, 1105
BciT130I CCWGG 2 cut(s) 200, 365
BcnI CCSGG 2 cut(s) 64, 856
BcoDI GTCTC 2 cut(s) 495, 756
BfaI CTAG 2 cut(s) 164, 426
BfmI CTRYAG 1 cut(s) 800
BfuAI ACCTGC 1 cut(s) 937
BisI GCNGC 4 cut(s) 143, 797, 800, 803
BlsI GCNGC 4 cut(s) 144, 798, 801, 804
Bme1390I CCNGG 4 cut(s) 64, 200, 365, 856
Bme18I GGWCC 1 cut(s) 664
BmeT110I CYCGRG 1 cut(s) 1082
BmgBI CACGTC 1 cut(s) 1094
BmgT120I GGNCC 2 cut(s) 150, 664
BmiI GGNNCC 3 cut(s) 6, 431, 1031
BmrFI CCNGG 4 cut(s) 64, 200, 365, 856
BmsI GCATC 5 cut(s) 68, 310, 646, 1096, 1154
BpiI GAAGAC 1 cut(s) 288
BpmI CTGGAG 1 cut(s) 1122
Bpu10I CCTNAGC 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 2 cut(s) 64, 856
BsaAI YACGTR 1 cut(s) 1133
BsaBI GATNNNNATC 1 cut(s) 467
BsaJI CCNNGG 3 cut(s) 198, 199, 660
Bsc4I CCNNNNNNNGG 1 cut(s) 370
Bse1I ACTGG 2 cut(s) 27, 568
Bse8I GATNNNNATC 1 cut(s) 467
BseBI CCWGG 2 cut(s) 200, 365
BseDI CCNNGG 3 cut(s) 198, 199, 660
BseGI GGATG 3 cut(s) 59, 661, 724
BseJI GATNNNNATC 1 cut(s) 467
BseLI CCNNNNNNNGG 1 cut(s) 370
BseMII CTCAG 3 cut(s) 108, 450, 1172
BseNI ACTGG 2 cut(s) 27, 568
BseRI GAGGAG 4 cut(s) 89, 92, 134, 1025
BseSI GKGCMC 1 cut(s) 586
BseXI GCAGC 4 cut(s) 154, 786, 808, 814
BshFI GGCC 5 cut(s) 152, 567, 770, 889, 992
BsiHKAI GWGCWC 3 cut(s) 41, 53, 586
BsiHKCI CYCGRG 1 cut(s) 1082
BsiSI CCGG 3 cut(s) 8, 63, 856
BslI CCNNNNNNNGG 1 cut(s) 370
BsmAI GTCTC 2 cut(s) 495, 756
BsmI GAATGC 1 cut(s) 229
BsnI GGCC 5 cut(s) 152, 567, 770, 889, 992
BsoBI CYCGRG 1 cut(s) 1082
Bsp1286I GDGCHC 3 cut(s) 41, 53, 586
Bsp143I GATC 6 cut(s) 90, 352, 628, 820, 836, 1176
BspACI CCGC 3 cut(s) 11, 32, 246
BspANI GGCC 5 cut(s) 152, 567, 770, 889, 992
BspCNI CTCAG 3 cut(s) 109, 451, 1171
BspLI GGNNCC 3 cut(s) 6, 431, 1031
BspMAI CTGCAG 1 cut(s) 804
BspMI ACCTGC 1 cut(s) 937
BspPI GGATC 2 cut(s) 85, 1184
BspQI GCTCTTC 2 cut(s) 729, 1047
BsrI ACTGG 2 cut(s) 27, 568
BssECI CCNNGG 3 cut(s) 198, 199, 660
BssMI GATC 6 cut(s) 90, 352, 628, 820, 836, 1176
BssT1I CCWWGG 1 cut(s) 660
Bst2UI CCWGG 2 cut(s) 200, 365
Bst4CI ACNGT 4 cut(s) 581, 694, 911, 980
Bst6I CTCTTC 5 cut(s) 363, 390, 722, 729, 1047
BstBAI YACGTR 1 cut(s) 1133
BstC8I GCNNGC 3 cut(s) 41, 114, 258
BstDEI CTNAG 3 cut(s) 117, 459, 1158
BstENI CCTNNNNNAGG 1 cut(s) 368
BstF5I GGATG 3 cut(s) 59, 661, 724
BstKTI GATC 6 cut(s) 93, 355, 631, 823, 839, 1179
BstMAI GTCTC 2 cut(s) 495, 756
BstMBI GATC 6 cut(s) 90, 352, 628, 820, 836, 1176
BstMWI GCNNNNNNNGC 2 cut(s) 122, 802
BstNI CCWGG 2 cut(s) 200, 365
BstNSI RCATGY 1 cut(s) 1154
BstSCI CCNGG 4 cut(s) 62, 198, 363, 854
BstSFI CTRYAG 1 cut(s) 800
BstSLI GKGCMC 1 cut(s) 586
BstV1I GCAGC 4 cut(s) 154, 786, 808, 814
BstV2I GAAGAC 1 cut(s) 288
BstX2I RGATCY 2 cut(s) 90, 1176
BstYI RGATCY 2 cut(s) 90, 1176
BsuRI GGCC 5 cut(s) 152, 567, 770, 889, 992
BtrI CACGTC 1 cut(s) 1094
BtsCI GGATG 3 cut(s) 59, 661, 724
BtsIMutI CAGTG 1 cut(s) 456
BveI ACCTGC 1 cut(s) 937
Cac8I GCNNGC 3 cut(s) 41, 114, 258
Cfr13I GGNCC 2 cut(s) 150, 664
Csp6I GTAC 2 cut(s) 171, 1130
CviAII CATG 7 cut(s) 71, 179, 241, 724, 766, 791, 1151
CviQI GTAC 2 cut(s) 171, 1130
DdeI CTNAG 3 cut(s) 117, 459, 1158
DpnI GATC 6 cut(s) 92, 354, 630, 822, 838, 1178
DpnII GATC 6 cut(s) 90, 352, 628, 820, 836, 1176
EaeI YGGCCR 2 cut(s) 565, 768
Eam1104I CTCTTC 5 cut(s) 363, 390, 722, 729, 1047
EarI CTCTTC 5 cut(s) 363, 390, 722, 729, 1047
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 2 cut(s) 39, 51
Eco130I CCWWGG 1 cut(s) 660
Eco24I GRGCYC 2 cut(s) 41, 53
Eco32I GATATC 1 cut(s) 382
Eco47I GGWCC 1 cut(s) 664
Eco53kI GAGCTC 2 cut(s) 39, 51
Eco57I CTGAAG 3 cut(s) 729, 1001, 1007
Eco88I CYCGRG 1 cut(s) 1082
EcoICRI GAGCTC 2 cut(s) 39, 51
EcoNI CCTNNNNNAGG 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 198, 363
EcoRV GATATC 1 cut(s) 382
EcoT14I CCWWGG 1 cut(s) 660
EcoT22I ATGCAT 1 cut(s) 325
EcoT38I GRGCYC 2 cut(s) 41, 53
ErhI CCWWGG 1 cut(s) 660
FaeI CATG 7 cut(s) 74, 182, 244, 727, 769, 794, 1154
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FatI CATG 7 cut(s) 70, 178, 240, 723, 765, 790, 1150
FblI GTMKAC 1 cut(s) 1088
Fnu4HI GCNGC 4 cut(s) 143, 797, 800, 803
FokI GGATG 3 cut(s) 46, 668, 731
FriOI GRGCYC 2 cut(s) 41, 53
Fsp4HI GCNGC 4 cut(s) 143, 797, 800, 803
FspBI CTAG 2 cut(s) 164, 426
GluI GCNGC 4 cut(s) 143, 797, 800, 803
GsuI CTGGAG 1 cut(s) 1122
HaeIII GGCC 5 cut(s) 152, 567, 770, 889, 992
HapII CCGG 3 cut(s) 8, 63, 856
Hin1II CATG 7 cut(s) 74, 182, 244, 727, 769, 794, 1154
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 4 cut(s) 468, 742, 1085, 1124
HpaII CCGG 3 cut(s) 8, 63, 856
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 3 cut(s) 454, 584, 1089
Hpy188I TCNGA 4 cut(s) 446, 944, 987, 1063
Hpy188III TCNNGA 9 cut(s) 86, 94, 356, 596, 818, 952, 1082, 1101, 1160
Hpy8I GTNNAC 3 cut(s) 454, 584, 1089
HpyAV CCTTC 3 cut(s) 49, 982, 1043
HpyCH4III ACNGT 4 cut(s) 581, 694, 911, 980
HpyCH4IV ACGT 2 cut(s) 1093, 1132
HpyCH4V TGCA 8 cut(s) 220, 260, 323, 387, 534, 584, 802, 1145
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 802
HpyF3I CTNAG 3 cut(s) 117, 459, 1158
HpySE526I ACGT 2 cut(s) 1093, 1132
Hsp92II CATG 7 cut(s) 74, 182, 244, 727, 769, 794, 1154
Kzo9I GATC 6 cut(s) 90, 352, 628, 820, 836, 1176
LguI GCTCTTC 2 cut(s) 729, 1047
LmnI GCTCC 6 cut(s) 10, 48, 435, 1012, 1084, 1114
Lsp1109I GCAGC 4 cut(s) 154, 786, 808, 814
LweI GCATC 5 cut(s) 68, 310, 646, 1096, 1154
MaeI CTAG 2 cut(s) 164, 426
MaeII ACGT 2 cut(s) 1093, 1132
MaeIII GTNAC 1 cut(s) 570
MalI GATC 6 cut(s) 92, 354, 630, 822, 838, 1178
MboI GATC 6 cut(s) 90, 352, 628, 820, 836, 1176
MboII GAAGA 9 cut(s) 241, 288, 380, 404, 407, 659, 739, 746, 1064
MflI RGATCY 2 cut(s) 90, 1176
MhlI GDGCHC 3 cut(s) 41, 53, 586
MlsI TGGCCA 2 cut(s) 567, 770
MluCI AATT 4 cut(s) 347, 529, 672, 807
MluNI TGGCCA 2 cut(s) 567, 770
MlyI GAGTC 2 cut(s) 1094, 1118
Mox20I TGGCCA 2 cut(s) 567, 770
Mph1103I ATGCAT 1 cut(s) 325
MroXI GAANNNNTTC 1 cut(s) 236
MscI TGGCCA 2 cut(s) 567, 770
MseI TTAA 3 cut(s) 107, 306, 972
MslI CAYNNNNRTG 1 cut(s) 461
Msp20I TGGCCA 2 cut(s) 567, 770
MspA1I CMGCKG 1 cut(s) 799
MspI CCGG 3 cut(s) 8, 63, 856
MspR9I CCNGG 4 cut(s) 64, 200, 365, 856
Mva1269I GAATGC 1 cut(s) 229
MvaI CCWGG 2 cut(s) 200, 365
MwoI GCNNNNNNNGC 2 cut(s) 122, 802
NciI CCSGG 2 cut(s) 64, 856
NdeII GATC 6 cut(s) 90, 352, 628, 820, 836, 1176
NlaIII CATG 7 cut(s) 74, 182, 244, 727, 769, 794, 1154
NlaIV GGNNCC 3 cut(s) 6, 431, 1031
NmeAIII GCCGAG 1 cut(s) 463
NsiI ATGCAT 1 cut(s) 325
NspI RCATGY 1 cut(s) 1154
PasI CCCWGGG 1 cut(s) 199
PciI ACATGT 1 cut(s) 1150
PciSI GCTCTTC 2 cut(s) 729, 1047
PctI GAATGC 1 cut(s) 229
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 2 cut(s) 468, 742
PfoI TCCNGGA 1 cut(s) 62
PkrI GCNGC 4 cut(s) 144, 798, 801, 804
PleI GAGTC 2 cut(s) 1093, 1118
PpsI GAGTC 2 cut(s) 1093, 1118
Ppu21I YACGTR 1 cut(s) 1133
PscI ACATGT 1 cut(s) 1150
Psp124BI GAGCTC 2 cut(s) 41, 53
Psp6I CCWGG 2 cut(s) 198, 363
PspGI CCWGG 2 cut(s) 198, 363
PspN4I GGNNCC 3 cut(s) 6, 431, 1031
PspPI GGNCC 2 cut(s) 150, 664
PstI CTGCAG 1 cut(s) 804
PsuI RGATCY 2 cut(s) 90, 1176
PvuII CAGCTG 1 cut(s) 799
RsaI GTAC 2 cut(s) 172, 1131
RsaNI GTAC 2 cut(s) 171, 1130
RseI CAYNNNNRTG 1 cut(s) 461
SacI GAGCTC 2 cut(s) 41, 53
SapI GCTCTTC 2 cut(s) 729, 1047
SaqAI TTAA 3 cut(s) 107, 306, 972
SatI GCNGC 4 cut(s) 143, 797, 800, 803
Sau3AI GATC 6 cut(s) 90, 352, 628, 820, 836, 1176
Sau96I GGNCC 2 cut(s) 150, 664
SchI GAGTC 2 cut(s) 1094, 1118
ScrFI CCNGG 4 cut(s) 64, 200, 365, 856
SduI GDGCHC 3 cut(s) 41, 53, 586
SfaNI GCATC 5 cut(s) 68, 310, 646, 1096, 1154
SfcI CTRYAG 1 cut(s) 800
SinI GGWCC 1 cut(s) 664
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 4 cut(s) 347, 529, 672, 807
SsiI CCGC 3 cut(s) 11, 32, 246
SspMI CTAG 2 cut(s) 164, 426
SstI GAGCTC 2 cut(s) 41, 53
StyD4I CCNGG 4 cut(s) 62, 198, 363, 854
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 4 cut(s) 581, 694, 911, 980
TaiI ACGT 2 cut(s) 1096, 1135
TaqI TCGA 2 cut(s) 27, 53
TasI AATT 4 cut(s) 347, 529, 672, 807
TfiI GAWTC 2 cut(s) 468, 742
Tru1I TTAA 3 cut(s) 107, 306, 972
Tru9I TTAA 3 cut(s) 107, 306, 972
TscAI CASTG 1 cut(s) 463
TseI GCWGC 4 cut(s) 142, 796, 799, 802
TspDTI ATGAA 4 cut(s) 229, 266, 420, 740
TspGWI ACGGA 1 cut(s) 977
TspRI CASTG 1 cut(s) 463
VneI GTGCAC 1 cut(s) 582
VpaK11BI GGWCC 1 cut(s) 664
XagI CCTNNNNNAGG 1 cut(s) 368
XapI RAATTY 1 cut(s) 347
XceI RCATGY 1 cut(s) 1154
XcmI CCANNNNNNNNNTGG 1 cut(s) 674
XmiI GTMKAC 1 cut(s) 1088
XmnI GAANNNNTTC 1 cut(s) 236
XspI CTAG 2 cut(s) 164, 426
Zsp2I ATGCAT 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.