Rroxscaffold_2G00131790

clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015501
Physical Location & Seq
Forward (+)
68586116 .. 68589196
3081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00131790.1

Sequence Viewer

Length: 1164 bp
ATGGGACGGAAAACAAAGATCAAAGACCTTCTGATGGGGGCAATCAAAGACAAAGCCTCTCAGAGCAAAGCAGCAATCCTCTTCACAATCAACACCGCTGCCAACAGTACTCCTCAATCATCTCAATCTCTCCACCTCGCCCTCCTCCGCGCTACCACCCACGACCCTTTCTCACCTCCACCAGCCAAACACCTTGCCGCGCTCCTCTCCTTCGGACACAGCTCGCGTGCCACTGCCTCCGCCGCCATCGAAGCCCTCATGGACCGCCTCCAAACCACCCAAAACTCCTCCGTCGCACTCAAGTGCCTCATCGCCGTTCACCACATCGTCAAGTACGGCTCCTTCATCCTCCAGGACCAGCTCTCCGTCTTCCCCTCCACTGGCGGCAGAAACTACCTTAAACTCTCCAACTTCCGGGACAACTCTAGCCCCATTTCCTGGGACCTCTCGTCCTGGGTCAGATGGTACGGACAGTACGTAGAAAACCTCATCATCACTTCTCGGTTCTTAGGTTTCTTCTTAGGATCGAACTCGTGCCTTGCCGATAGAGACAGACAAGAAGAGAGAATCTCAGCGCTCATGAATTCCGACCTGGTTAGGGAGACCGAGTCGCTGGTGAGTCTAATGGAAGAGATTTGCCGGCGGCCTGACTTTTCAAGTTTGAACGGTAACAGGTTGGTCGGGGAGATATTGGGTTTGGTGGGTCAGGACCAGATTGCCGCAATGAACGAGGTTTCGGTCCGAGTCAAGGAGTTTGATGAGAGACTGAGTTGCATGAGCTTTGGCGACTCGGTCGAGTTGCTGAGCACGTTAAAGAGGTCGGAGGATTGCAAGGAGAGGCTTCTGACAGTGTCGTCGGCGGTGAAGAGTGATTTGGTAGAGAGGTTTTGGGGGTTGATGAGAAAGATGAATGGAAGGGTTAAGAAAGAGAAAGAGAAAGAGGGTGGTAAGTTGGTGGTGATGGCGGTGAGGAGAGACAGTGTGGGTGAGTCGGCTCGCTTTGATGACCGAGTTTTAAGGTCGTATGACTCGGTCCGATTCTCCTCAGGAAGGTTGAAGGACCGATCCACCAAAAACCCATGGTTGGAGAAGCAAAGTCTTATAATTGGCCCAAAAAACTTTTGGCACCCAATGGTTGGAGTTGCCAGGAAGCTAGACCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

387

Amino Acids

43.32

Weight (kDa)

9.56

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 45 - 281 3.5e-15 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015501)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G40080
fragaria_vesca FvH4_1g20100
malus_domestica MD15G1316600.v1.1
prunus_persica Prupe.6G195400_v2.0.a1
pyrus_communis pycom15g27940
rosa_chinensis RchiOBHm_Chr2g0112101
rosa_laevigata RLG00000017950
rosa_multiflora Rmu_sc0000360.1_g000002 Rmu_sc0001294.1_g000002
rosa_roxburghii Rroxscaffold_2G00131790
rosa_rugosa Rorug02G0178400
rosa_samantha Rh2AG230400 Rh2BG243600 Rh2CG234900 Rh2DG238400
rosa_wichuraiana Rw2G017850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1103
AasI GACNNNNNNGTC 1 cut(s) 853
AccB1I GGYRCC 1 cut(s) 1125
AccB7I CCANNNNNTGG 2 cut(s) 438, 1136
AccII CGCG 3 cut(s) 150, 200, 226
AclWI GGATC 2 cut(s) 532, 1059
AcsI RAATTY 1 cut(s) 583
AfaI GTAC 4 cut(s) 109, 335, 467, 476
AfeI AGCGCT 1 cut(s) 576
AfiI CCNNNNNNNGG 9 cut(s) 34, 380, 414, 438, 598, 748, 1050, 1084, 1136
AgsI TTSAA 3 cut(s) 657, 664, 1057
AhdI GACNNNNNGTC 1 cut(s) 448
AjnI CCWGG 5 cut(s) 351, 437, 452, 591, 1145
AjuI GAANNNNNNNTTGG 2 cut(s) 857, 889
AleI CACNNNNGTG 1 cut(s) 301
AluBI AGCT 4 cut(s) 222, 361, 780, 1153
AluI AGCT 4 cut(s) 222, 361, 780, 1153
Alw21I GWGCWC 1 cut(s) 809
Alw26I GTCTC 4 cut(s) 543, 596, 757, 969
AlwI GGATC 2 cut(s) 532, 1059
Aor51HI AGCGCT 1 cut(s) 576
AoxI GGCC 2 cut(s) 644, 1108
ApeKI GCWGC 2 cut(s) 71, 98
ApoI RAATTY 1 cut(s) 583
AspLEI GCGC 3 cut(s) 152, 202, 577
AspS9I GGNCC 8 cut(s) 262, 355, 442, 709, 739, 1033, 1060, 1109
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 7 cut(s) 165, 311, 628, 874, 970, 979, 998
AvaII GGWCC 7 cut(s) 262, 355, 442, 709, 739, 1033, 1060
AxyI CCTNAGG 1 cut(s) 1045
BanI GGYRCC 1 cut(s) 1125
BarI GAAGNNNNNNTAC 2 cut(s) 326, 358
BauI CACGAG 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 809
BbvI GCAGC 2 cut(s) 83, 85
BccI CCATC 4 cut(s) 28, 254, 456, 955
BceAI ACGGC 2 cut(s) 299, 352
BciT130I CCWGG 5 cut(s) 353, 439, 454, 593, 1147
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 4 cut(s) 543, 596, 757, 969
BfaI CTAG 2 cut(s) 426, 1154
BfoI RGCGCY 1 cut(s) 578
BisI GCNGC 7 cut(s) 72, 99, 198, 243, 385, 644, 720
BlpI GCTNAGC 1 cut(s) 803
BlsI GCNGC 7 cut(s) 73, 100, 199, 244, 386, 645, 721
BmcAI AGTACT 1 cut(s) 109
Bme1390I CCNGG 6 cut(s) 353, 416, 439, 454, 593, 1147
Bme18I GGWCC 7 cut(s) 262, 355, 442, 709, 739, 1033, 1060
BmeRI GACNNNNNGTC 1 cut(s) 448
BmgT120I GGNCC 8 cut(s) 262, 355, 442, 709, 739, 1033, 1060, 1109
BmiI GGNNCC 3 cut(s) 340, 443, 1127
BmrFI CCNGG 6 cut(s) 353, 416, 439, 454, 593, 1147
BpiI GAAGAC 1 cut(s) 361
BplI GAGNNNNNCTC 2 cut(s) 554, 586
BpmI CTGGAG 1 cut(s) 335
Bpu1102I GCTNAGC 1 cut(s) 803
BpuEI CTTGAG 1 cut(s) 284
BpuMI CCSGG 1 cut(s) 416
BsaAI YACGTR 1 cut(s) 478
BsaI GGTCTC 1 cut(s) 596
BsaJI CCNNGG 3 cut(s) 438, 453, 1079
BsaXI ACNNNNNCTCC 6 cut(s) 347, 377, 593, 623, 964, 994
Bsc4I CCNNNNNNNGG 9 cut(s) 34, 380, 414, 438, 598, 748, 1050, 1084, 1136
Bse118I RCCGGY 1 cut(s) 639
Bse1I ACTGG 1 cut(s) 385
Bse21I CCTNAGG 1 cut(s) 1045
Bse3DI GCAATG 1 cut(s) 729
BseBI CCWGG 5 cut(s) 353, 439, 454, 593, 1147
BseDI CCNNGG 3 cut(s) 438, 453, 1079
BseGI GGATG 1 cut(s) 345
BseLI CCNNNNNNNGG 9 cut(s) 34, 380, 414, 438, 598, 748, 1050, 1084, 1136
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 5 cut(s) 74, 585, 758, 794, 1059
BseNI ACTGG 1 cut(s) 385
BseRI GAGGAG 6 cut(s) 102, 134, 194, 277, 985, 1033
BseXI GCAGC 2 cut(s) 83, 85
Bsh1236I CGCG 3 cut(s) 150, 200, 226
Bsh1285I CGRYCG 1 cut(s) 795
BshFI GGCC 2 cut(s) 646, 1110
BshNI GGYRCC 1 cut(s) 1125
BsiEI CGRYCG 1 cut(s) 795
BsiHKAI GWGCWC 1 cut(s) 809
BsiSI CCGG 2 cut(s) 415, 640
BslFI GGGAC 3 cut(s) 18, 431, 455
BslI CCNNNNNNNGG 9 cut(s) 34, 380, 414, 438, 598, 748, 1050, 1084, 1136
BsmAI GTCTC 4 cut(s) 543, 596, 757, 969
BsmFI GGGAC 3 cut(s) 18, 431, 455
BsnI GGCC 2 cut(s) 646, 1110
Bso31I GGTCTC 1 cut(s) 596
Bsp1286I GDGCHC 1 cut(s) 809
Bsp143I GATC 3 cut(s) 18, 524, 1064
Bsp1720I GCTNAGC 1 cut(s) 803
Bsp19I CCATGG 1 cut(s) 1079
BspANI GGCC 2 cut(s) 646, 1110
BspCNI CTCAG 5 cut(s) 73, 584, 759, 795, 1058
BspFNI CGCG 3 cut(s) 150, 200, 226
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 3 cut(s) 340, 443, 1127
BspPI GGATC 2 cut(s) 532, 1059
BspT107I GGYRCC 1 cut(s) 1125
BspTNI GGTCTC 1 cut(s) 596
BsrDI GCAATG 1 cut(s) 729
BsrFI RCCGGY 1 cut(s) 639
BsrI ACTGG 1 cut(s) 385
BssAI RCCGGY 1 cut(s) 639
BssECI CCNNGG 3 cut(s) 438, 453, 1079
BssMI GATC 3 cut(s) 18, 524, 1064
BssSI CACGAG 1 cut(s) 532
BssT1I CCWWGG 1 cut(s) 1079
Bst2BI CACGAG 1 cut(s) 532
Bst2UI CCWGG 5 cut(s) 353, 439, 454, 593, 1147
Bst4CI ACNGT 5 cut(s) 107, 474, 668, 850, 980
Bst6I CTCTTC 4 cut(s) 86, 555, 624, 860
BstBAI YACGTR 1 cut(s) 478
BstC8I GCNNGC 4 cut(s) 224, 228, 641, 997
BstDEI CTNAG 7 cut(s) 60, 508, 520, 571, 767, 803, 1045
BstDSI CCRYGG 1 cut(s) 1079
BstENI CCTNNNNNAGG 1 cut(s) 1048
BstF5I GGATG 1 cut(s) 345
BstFNI CGCG 3 cut(s) 150, 200, 226
BstH2I RGCGCY 1 cut(s) 578
BstHHI GCGC 3 cut(s) 152, 202, 577
BstKTI GATC 3 cut(s) 21, 527, 1067
BstMAI GTCTC 4 cut(s) 543, 596, 757, 969
BstMBI GATC 3 cut(s) 18, 524, 1064
BstMCI CGRYCG 1 cut(s) 795
BstMWI GCNNNNNNNGC 2 cut(s) 242, 251
BstNI CCWGG 5 cut(s) 353, 439, 454, 593, 1147
BstSCI CCNGG 6 cut(s) 351, 414, 437, 452, 591, 1145
BstSNI TACGTA 1 cut(s) 478
BstUI CGCG 3 cut(s) 150, 200, 226
BstV1I GCAGC 2 cut(s) 83, 85
BstV2I GAAGAC 1 cut(s) 361
Bsu36I CCTNAGG 1 cut(s) 1045
BsuRI GGCC 2 cut(s) 646, 1110
BtgI CCRYGG 1 cut(s) 1079
BtgZI GCGATG 1 cut(s) 295
BtsCI GGATG 1 cut(s) 345
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 4 cut(s) 231, 378, 855, 985
Cac8I GCNNGC 4 cut(s) 224, 228, 641, 997
CciI TCATGA 1 cut(s) 579
CfoI GCGC 3 cut(s) 152, 202, 577
Cfr10I RCCGGY 1 cut(s) 639
Cfr13I GGNCC 8 cut(s) 262, 355, 442, 709, 739, 1033, 1060, 1109
CpoI CGGWCCG 2 cut(s) 739, 1033
CsiI ACCWGGT 1 cut(s) 591
Csp6I GTAC 4 cut(s) 108, 334, 466, 475
CspI CGGWCCG 2 cut(s) 739, 1033
CviAII CATG 4 cut(s) 259, 580, 775, 1080
CviQI GTAC 4 cut(s) 108, 334, 466, 475
DdeI CTNAG 7 cut(s) 60, 508, 520, 571, 767, 803, 1045
DpnI GATC 3 cut(s) 20, 526, 1066
DpnII GATC 3 cut(s) 18, 524, 1064
DrdI GACNNNNNNGTC 1 cut(s) 853
DriI GACNNNNNGTC 1 cut(s) 448
DseDI GACNNNNNNGTC 1 cut(s) 853
Eam1104I CTCTTC 4 cut(s) 86, 555, 624, 860
Eam1105I GACNNNNNGTC 1 cut(s) 448
EarI CTCTTC 4 cut(s) 86, 555, 624, 860
EciI GGCGGA 1 cut(s) 229
Eco105I TACGTA 1 cut(s) 478
Eco130I CCWWGG 1 cut(s) 1079
Eco31I GGTCTC 1 cut(s) 596
Eco47I GGWCC 7 cut(s) 262, 355, 442, 709, 739, 1033, 1060
Eco47III AGCGCT 1 cut(s) 576
Eco81I CCTNAGG 1 cut(s) 1045
EcoNI CCTNNNNNAGG 1 cut(s) 1048
EcoO109I RGGNCCY 1 cut(s) 442
EcoRI GAATTC 1 cut(s) 583
EcoRII CCWGG 5 cut(s) 351, 437, 452, 591, 1145
EcoT14I CCWWGG 1 cut(s) 1079
ErhI CCWWGG 1 cut(s) 1079
FaeI CATG 4 cut(s) 262, 583, 778, 1083
FaiI YATR 7 cut(s) 260, 581, 776, 1026, 1081, 1103, 1162
FaqI GGGAC 3 cut(s) 18, 431, 455
FatI CATG 4 cut(s) 258, 579, 774, 1079
Fnu4HI GCNGC 7 cut(s) 72, 99, 198, 243, 385, 644, 720
FokI GGATG 1 cut(s) 332
Fsp4HI GCNGC 7 cut(s) 72, 99, 198, 243, 385, 644, 720
FspBI CTAG 2 cut(s) 426, 1154
GlaI GCGC 3 cut(s) 151, 201, 576
GluI GCNGC 7 cut(s) 72, 99, 198, 243, 385, 644, 720
GsuI CTGGAG 1 cut(s) 335
HaeII RGCGCY 1 cut(s) 578
HaeIII GGCC 2 cut(s) 646, 1110
HapII CCGG 2 cut(s) 415, 640
HhaI GCGC 3 cut(s) 152, 202, 577
Hin1II CATG 4 cut(s) 262, 583, 778, 1083
Hin6I GCGC 3 cut(s) 150, 200, 575
HinP1I GCGC 3 cut(s) 150, 200, 575
HinfI GANTC 8 cut(s) 567, 608, 619, 744, 788, 989, 1028, 1038
HpaII CCGG 2 cut(s) 415, 640
HphI GGTGA 7 cut(s) 165, 311, 628, 874, 970, 979, 998
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 9 cut(s) 33, 63, 215, 461, 589, 743, 823, 846, 1037
Hpy188III TCNNGA 3 cut(s) 580, 707, 1047
Hpy8I GTNNAC 1 cut(s) 319
Hpy99I CGWCG 2 cut(s) 296, 859
HpyAV CCTTC 6 cut(s) 38, 220, 352, 909, 1044, 1051
HpyCH4III ACNGT 5 cut(s) 107, 474, 668, 850, 980
HpyCH4IV ACGT 2 cut(s) 477, 809
HpyCH4V TGCA 2 cut(s) 774, 831
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 251
HpyF3I CTNAG 7 cut(s) 60, 508, 520, 571, 767, 803, 1045
HpySE526I ACGT 2 cut(s) 477, 809
Hsp92II CATG 4 cut(s) 262, 583, 778, 1083
HspAI GCGC 3 cut(s) 150, 200, 575
KroI GCCGGC 1 cut(s) 639
KroNI GCCGGC 1 cut(s) 641
Kzo9I GATC 3 cut(s) 18, 524, 1064
LmnI GCTCC 2 cut(s) 207, 344
Lsp1109I GCAGC 2 cut(s) 83, 85
MabI ACCWGGT 1 cut(s) 591
MaeI CTAG 2 cut(s) 426, 1154
MaeII ACGT 2 cut(s) 477, 809
MaeIII GTNAC 1 cut(s) 668
MalI GATC 3 cut(s) 20, 526, 1066
MboI GATC 3 cut(s) 18, 524, 1064
MboII GAAGA 6 cut(s) 73, 361, 508, 572, 641, 877
MhlI GDGCHC 1 cut(s) 809
MluCI AATT 2 cut(s) 583, 1104
MlyI GAGTC 6 cut(s) 617, 628, 753, 782, 998, 1022
MmeI TCCRAC 5 cut(s) 432, 612, 801, 1065, 1117
MroNI GCCGGC 1 cut(s) 639
MseI TTAA 4 cut(s) 399, 812, 921, 1016
MslI CAYNNNNRTG 1 cut(s) 301
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 2 cut(s) 415, 640
MspR9I CCNGG 6 cut(s) 353, 416, 439, 454, 593, 1147
MvaI CCWGG 5 cut(s) 353, 439, 454, 593, 1147
MvnI CGCG 3 cut(s) 150, 200, 226
MwoI GCNNNNNNNGC 2 cut(s) 242, 251
NaeI GCCGGC 1 cut(s) 641
NciI CCSGG 1 cut(s) 416
NcoI CCATGG 1 cut(s) 1079
NdeII GATC 3 cut(s) 18, 524, 1064
NgoMIV GCCGGC 1 cut(s) 639
NlaIII CATG 4 cut(s) 262, 583, 778, 1083
NlaIV GGNNCC 3 cut(s) 340, 443, 1127
OliI CACNNNNGTG 1 cut(s) 301
PagI TCATGA 1 cut(s) 579
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PdiI GCCGGC 1 cut(s) 641
PfeI GAWTC 2 cut(s) 567, 1038
PflFI GACNNNGTC 4 cut(s) 607, 791, 850, 1031
PflMI CCANNNNNTGG 2 cut(s) 438, 1136
PfoI TCCNGGA 2 cut(s) 351, 414
PkrI GCNGC 7 cut(s) 73, 100, 199, 244, 386, 645, 721
PleI GAGTC 6 cut(s) 616, 627, 752, 782, 997, 1022
PpsI GAGTC 6 cut(s) 616, 627, 752, 782, 997, 1022
Ppu21I YACGTR 1 cut(s) 478
PpuMI RGGWCCY 1 cut(s) 442
PsiI TTATAA 1 cut(s) 1103
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 5 cut(s) 351, 437, 452, 591, 1145
PspGI CCWGG 5 cut(s) 351, 437, 452, 591, 1145
PspN4I GGNNCC 3 cut(s) 340, 443, 1127
PspPI GGNCC 8 cut(s) 262, 355, 442, 709, 739, 1033, 1060, 1109
PspPPI RGGWCCY 1 cut(s) 442
PsyI GACNNNGTC 4 cut(s) 607, 791, 850, 1031
RsaI GTAC 4 cut(s) 109, 335, 467, 476
RsaNI GTAC 4 cut(s) 108, 334, 466, 475
RseI CAYNNNNRTG 1 cut(s) 301
Rsr2I CGGWCCG 2 cut(s) 739, 1033
RsrII CGGWCCG 2 cut(s) 739, 1033
SaqAI TTAA 4 cut(s) 399, 812, 921, 1016
SatI GCNGC 7 cut(s) 72, 99, 198, 243, 385, 644, 720
Sau3AI GATC 3 cut(s) 18, 524, 1064
Sau96I GGNCC 8 cut(s) 262, 355, 442, 709, 739, 1033, 1060, 1109
ScaI AGTACT 1 cut(s) 109
SchI GAGTC 6 cut(s) 617, 628, 753, 782, 998, 1022
ScrFI CCNGG 6 cut(s) 353, 416, 439, 454, 593, 1147
SduI GDGCHC 1 cut(s) 809
SexAI ACCWGGT 1 cut(s) 591
SinI GGWCC 7 cut(s) 262, 355, 442, 709, 739, 1033, 1060
SmiMI CAYNNNNRTG 1 cut(s) 301
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
SnaBI TACGTA 1 cut(s) 478
Sse9I AATT 2 cut(s) 583, 1104
SspMI CTAG 2 cut(s) 426, 1154
StyD4I CCNGG 6 cut(s) 351, 414, 437, 452, 591, 1145
StyI CCWWGG 1 cut(s) 1079
TaaI ACNGT 5 cut(s) 107, 474, 668, 850, 980
TaiI ACGT 2 cut(s) 480, 812
TaqI TCGA 3 cut(s) 249, 527, 795
TaqII GACCGA 6 cut(s) 620, 727, 781, 1021, 1023, 1077
TasI AATT 2 cut(s) 583, 1104
TatI WGTACW 1 cut(s) 107
TauI GCSGC 5 cut(s) 200, 245, 387, 646, 722
TfiI GAWTC 2 cut(s) 567, 1038
Tru1I TTAA 4 cut(s) 399, 812, 921, 1016
Tru9I TTAA 4 cut(s) 399, 812, 921, 1016
TscAI CASTG 4 cut(s) 238, 385, 855, 985
TseI GCWGC 2 cut(s) 71, 98
TspDTI ATGAA 4 cut(s) 334, 596, 740, 923
TspGWI ACGGA 4 cut(s) 22, 280, 355, 483
TspRI CASTG 4 cut(s) 238, 385, 855, 985
Tth111I GACNNNGTC 4 cut(s) 607, 791, 850, 1031
Van91I CCANNNNNTGG 2 cut(s) 438, 1136
VpaK11BI GGWCC 7 cut(s) 262, 355, 442, 709, 739, 1033, 1060
XagI CCTNNNNNAGG 1 cut(s) 1048
XapI RAATTY 1 cut(s) 583
XcmI CCANNNNNNNNNTGG 1 cut(s) 1119
XspI CTAG 2 cut(s) 426, 1154
ZrmI AGTACT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.