Rroxscaffold_2G00136440

Late embryogenesis abundant protein 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014508
Physical Location & Seq
Forward (+)
74131867 .. 74132139
273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136440.1

Sequence Viewer

Length: 273 bp
ATGGCGAGCAAGCCAGGAGGAGGAGAGAGAAAGCCAGCAGCTGATCAGGGAAGCGATGCGAAGTTAGAGCAGGCAATGAAGGACAGTCCGTACGTCCAATCCGATAACTTGGAGGATTACAAGCGCCAGGGGTACGGAACCGACGGACATCAACAGGTCCAGCCGGGTCGGGGCGCCGGATCCACCGATGCTCCGACTGTTTCCGGGGCTAATGTTTCCTCCAAGGCGGATGTTACCGCCACTGAAACTATCAACCGCCAAGGAGTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_6

Protein Analysis

90

Amino Acids

9.34

Weight (kDa)

5.2

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_6 PF10714 11 - 87 4.5e-29 Late embryogenesis abundant protein 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23110 AT2G23110 AT2G33690
fragaria_vesca FvH4_1g17140
malus_domestica MD02G1181800.v1.1 MD15G1292700.v1.1
prunus_persica Prupe.6G224700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0107321
rosa_multiflora Rmu_sc0006416.1_g000001
rosa_roxburghii Rroxscaffold_2G00136440
rosa_rugosa Rorug02G0141900.1 Rorug02G0142000
rosa_samantha Rh2AG192700 Rh2BG204800 Rh2CG197900 Rh2DG199900
rosa_wichuraiana Rw2G015260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AciI CCGC 3 cut(s) 227, 237, 256
AclWI GGATC 2 cut(s) 174, 187
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 2 cut(s) 92, 134
AfiI CCNNNNNNNGG 2 cut(s) 20, 170
AjnI CCWGG 2 cut(s) 13, 126
AluBI AGCT 1 cut(s) 41
AluI AGCT 1 cut(s) 41
AlwI GGATC 2 cut(s) 174, 187
AlwNI CAGNNNCTG 1 cut(s) 41
ApeKI GCWGC 1 cut(s) 38
AspLEI GCGC 2 cut(s) 126, 176
AspS9I GGNCC 1 cut(s) 157
AsuC2I CCSGG 2 cut(s) 165, 205
AvaII GGWCC 1 cut(s) 157
BamHI GGATCC 1 cut(s) 179
BanI GGYRCC 1 cut(s) 173
BbvI GCAGC 1 cut(s) 50
BciT130I CCWGG 2 cut(s) 15, 128
BclI TGATCA 1 cut(s) 43
BcnI CCSGG 2 cut(s) 165, 205
BfoI RGCGCY 2 cut(s) 127, 177
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme1390I CCNGG 4 cut(s) 15, 128, 165, 205
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 3 cut(s) 139, 175, 181
BmrFI CCNGG 4 cut(s) 15, 128, 165, 205
BmsI GCATC 2 cut(s) 46, 178
BpuMI CCSGG 2 cut(s) 165, 205
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 4 cut(s) 127, 204, 222, 259
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 170
Bse3DI GCAATG 1 cut(s) 81
BseBI CCWGG 2 cut(s) 15, 128
BseDI CCNNGG 4 cut(s) 127, 204, 222, 259
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 2 cut(s) 20, 170
BseMI GCAATG 1 cut(s) 81
BseRI GAGGAG 2 cut(s) 33, 36
BseXI GCAGC 1 cut(s) 50
BshNI GGYRCC 1 cut(s) 173
BsiSI CCGG 3 cut(s) 164, 177, 204
BsiWI CGTACG 1 cut(s) 90
BslI CCNNNNNNNGG 2 cut(s) 20, 170
Bsp143I GATC 2 cut(s) 43, 179
BspACI CCGC 3 cut(s) 227, 237, 256
BspLI GGNNCC 3 cut(s) 139, 175, 181
BspPI GGATC 2 cut(s) 174, 187
BspT107I GGYRCC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 81
BssECI CCNNGG 4 cut(s) 127, 204, 222, 259
BssMI GATC 2 cut(s) 43, 179
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 2 cut(s) 222, 259
Bst2UI CCWGG 2 cut(s) 15, 128
Bst4CI ACNGT 2 cut(s) 86, 199
BstACI GRCGYC 1 cut(s) 174
BstC8I GCNNGC 4 cut(s) 7, 11, 36, 72
BstF5I GGATG 1 cut(s) 235
BstH2I RGCGCY 2 cut(s) 127, 177
BstHHI GCGC 2 cut(s) 126, 176
BstKTI GATC 2 cut(s) 46, 182
BstMBI GATC 2 cut(s) 43, 179
BstNI CCWGG 2 cut(s) 15, 128
BstSCI CCNGG 4 cut(s) 13, 126, 163, 203
BstV1I GCAGC 1 cut(s) 50
BstX2I RGATCY 1 cut(s) 179
BstYI RGATCY 1 cut(s) 179
BtgZI GCGATG 1 cut(s) 69
BtsCI GGATG 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 4 cut(s) 7, 11, 36, 72
CaiI CAGNNNCTG 1 cut(s) 41
CfoI GCGC 2 cut(s) 126, 176
Cfr13I GGNCC 1 cut(s) 157
Csp6I GTAC 2 cut(s) 91, 133
CviJI RGCY 5 cut(s) 13, 34, 41, 163, 209
CviKI_1 RGCY 5 cut(s) 13, 34, 41, 163, 209
CviQI GTAC 2 cut(s) 91, 133
DinI GGCGCC 1 cut(s) 175
DpnI GATC 2 cut(s) 45, 181
DpnII GATC 2 cut(s) 43, 179
EciI GGCGGA 1 cut(s) 242
Eco130I CCWWGG 2 cut(s) 222, 259
Eco47I GGWCC 1 cut(s) 157
EcoRII CCWGG 2 cut(s) 13, 126
EcoT14I CCWWGG 2 cut(s) 222, 259
EgeI GGCGCC 1 cut(s) 175
EheI GGCGCC 1 cut(s) 175
ErhI CCWWGG 2 cut(s) 222, 259
FbaI TGATCA 1 cut(s) 43
Fnu4HI GCNGC 1 cut(s) 39
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 1 cut(s) 39
GlaI GCGC 2 cut(s) 125, 175
GluI GCNGC 1 cut(s) 39
HaeII RGCGCY 2 cut(s) 127, 177
HapII CCGG 3 cut(s) 164, 177, 204
HhaI GCGC 2 cut(s) 126, 176
Hin1I GRCGYC 1 cut(s) 174
Hin6I GCGC 2 cut(s) 124, 174
HinP1I GCGC 2 cut(s) 124, 174
HpaII CCGG 3 cut(s) 164, 177, 204
Hpy188I TCNGA 2 cut(s) 103, 195
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 73
HpyCH4III ACNGT 2 cut(s) 86, 199
HpyCH4IV ACGT 1 cut(s) 93
HpySE526I ACGT 1 cut(s) 93
Hsp92I GRCGYC 1 cut(s) 174
HspAI GCGC 2 cut(s) 124, 174
KasI GGCGCC 1 cut(s) 173
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 2 cut(s) 43, 179
LmnI GCTCC 1 cut(s) 196
Lsp1109I GCAGC 1 cut(s) 50
LweI GCATC 2 cut(s) 46, 178
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 1 cut(s) 232
MalI GATC 2 cut(s) 45, 181
MboI GATC 2 cut(s) 43, 179
MflI RGATCY 1 cut(s) 179
MluCI AATT 1 cut(s) 268
Mly113I GGCGCC 1 cut(s) 174
MmeI TCCRAC 1 cut(s) 218
MnlI CCTC 4 cut(s) 11, 14, 106, 229
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 41
MspI CCGG 3 cut(s) 164, 177, 204
MspR9I CCNGG 4 cut(s) 15, 128, 165, 205
MvaI CCWGG 2 cut(s) 15, 128
NarI GGCGCC 1 cut(s) 174
NciI CCSGG 2 cut(s) 165, 205
NdeII GATC 2 cut(s) 43, 179
NlaIV GGNNCC 3 cut(s) 139, 175, 181
PcsI WCGNNNNNNNCGW 1 cut(s) 99
Pfl23II CGTACG 1 cut(s) 90
PkrI GCNGC 1 cut(s) 40
PluTI GGCGCC 1 cut(s) 177
Psp6I CCWGG 2 cut(s) 13, 126
PspGI CCWGG 2 cut(s) 13, 126
PspLI CGTACG 1 cut(s) 90
PspN4I GGNNCC 3 cut(s) 139, 175, 181
PspPI GGNCC 1 cut(s) 157
PstNI CAGNNNCTG 1 cut(s) 41
PsuI RGATCY 1 cut(s) 179
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 2 cut(s) 92, 134
RsaNI GTAC 2 cut(s) 91, 133
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 1 cut(s) 39
Sau3AI GATC 2 cut(s) 43, 179
Sau96I GGNCC 1 cut(s) 157
ScrFI CCNGG 4 cut(s) 15, 128, 165, 205
SetI ASST 3 cut(s) 43, 96, 159
SfaNI GCATC 2 cut(s) 46, 178
SfoI GGCGCC 1 cut(s) 175
SinI GGWCC 1 cut(s) 157
Sse9I AATT 1 cut(s) 268
SsiI CCGC 3 cut(s) 227, 237, 256
SspDI GGCGCC 1 cut(s) 173
StyD4I CCNGG 4 cut(s) 13, 126, 163, 203
StyI CCWWGG 2 cut(s) 222, 259
TaaI ACNGT 2 cut(s) 86, 199
TaiI ACGT 1 cut(s) 96
TasI AATT 1 cut(s) 268
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 247
TseI GCWGC 1 cut(s) 38
TspDTI ATGAA 1 cut(s) 92
TspGWI ACGGA 3 cut(s) 78, 150, 159
TspRI CASTG 1 cut(s) 247
VpaK11BI GGWCC 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.