Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0012807

Physical Location & Seq

Genomic Coordinates Forward (+)

85137304 .. 85140207

Gene Structure 2904 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00147210.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2052 bp

ATGCCGGTGCAACTGACGCCGCACGCGGTATCCGCCATCATCGGCGGTGATCTCAACTTGAAGCCGCTGATGCAGGTCACCGATATCAAGCTCATCGGCTCGCAGGAGAGGTACCGGCTCATGGTCTCCGACGGCGTTTGGTCTCAGCACGCCATGGTCGCCACTCAGCTCAACGAGCTAGTCAAGACCGGCCAGGTCAGGAAAGGCTCCGTCGTCCAGCTTACCGAGTACATTTGCAGCGAAGTCCAGAACCGCAACAAGTTTGTGGCCGGTTTTGACCTTGAACGCTTGTACAGGATTATTGTTGTGCTGAATATGGAAACTATTGTACTGGACTCGGAGATTATTGGGAATCCAAAACAATATGTTGAGTCTGGTCCAACTGCTGAAACTGCATTGCCTAAGAACAATTTTCAGCAGTCTGCCTATGGCAACAATCATATGATTGGCCAAGGGCCTGTTCCCAAATGGCAGAATCCCAGGCCCTCTTTTCATCCTGAAAACTTTGTTTCCAGTATGCCAAATGCCAGACCTACATTCCAATCTGAAAGCTCTTTTTACAACACTCCGAATCCCAGACCTACATTTCGTCCTGAAAGCTCAGCTCCCAGTGTGCCAAATTTCCGAGCTACTGTGCAGCCTTCCTACCAGCCACCTCCACAGTATAAAAATCGTGGCCCTGTGTTCAAGAATGAGGCGCCAGCACGTATTATACCTATTAATGCTCTAAATCCTTATCAGGGAAGGTGGGCTATCAAGGCAAGAGTCACTGCAAAAGGGGATCAGCGTCGGTATAACAATGCTCGAGGAGATGGTAAGGTTTTCTCTTTCGACCTCCTTGATTCTGAGGGTGGGGAAATACGAGTGACCTGCTTTAATGCCGTTCTTGACCGCTTTTATGATATTATTGAGGTTGGCAAAGTTTACTTGATATCAAAGGGTAGCTTAAAACCTGCGCAGAAGAACTTTAATCATCTGAAAAGTGACTGGGAGATAACTCTGGATGCAAGTTCAACAGTGGAACTGTGCCCTGATGAAGATGGTTCTATACCAGACCAGCTGTTTGACTTCAAGCCTATTAGTGCAATTGAGAATGCTGAGAGTAATTCTATACTTGATGTTATTGGCATTGTGACATCTGTCAACCCTTCTACTACTATCCTGAGAAAGAATGGTATGGAAACTCAGAGAAGAATTCTGCATCTGAAGGATTGGTCTGGTAGGAGCGTTGAGATAACCCTATGGGGGGAGGTCTGTAACAGGGAAGGTCAAAAGCTGGAAGAGATGTTGGCTTCTGGTGTTTTTCCAGTTTTAGCTGTCAAAGCTGGGAAGGTTAATGACTTCAGTGGGAAATCCATAGGAACAATTCATTCTACCCAGCTATACATTAACCCAGATTTGCCCGAGGCTCGTCAGGTGAGGGACTGGTTTGATCAAGGGGGAAAGGATGCCGCTTCCATGTCCATTTCTAAGGAATTTATGCCAGGAGGAGTAACTAAGAATGACATACGCAAAACTGTTTCTCAGATCAAGAATGAAGGTCTTGGAAGATCAGATAAGCCAGACTGGGTCACTGTGAGGGCCACCATATCTTTCATTAAAACCGATTCTTTCTGTTACACAGCATGCCCGTTAATGATTGGAGATAGACAGTGTAGCAAGAAAGTGTCGAGGTCAGGAGTTGGAGGATGGCAGTGTGACAGGTGCAATCAAGAATTTGAGGAGTGTGATTATAGATATCTTCTTCAAGCCCAAATTCAGGACCATACTGGATTGACTTGGGTTACGGCTTTTCAGGAATCAGGGGCAGAGATCTTGGGTTGCACAGCAAAGGAGTTGTACTTTTTGAAATACGAGGGGCAGGATGAGGAAAAGTTCACAGACATAGTCCGGAGCAGTATTTTCAACCAGTTTATGTTCAAGCTTAAGATCAAAGAGGAGTTGTATGGCGACGAGCAGAAGGTGAAGATCACAGTGGTTAAGGCAGATACGGTGAACTATTCTGCAGAGAGCAGATACATGCTTGATGTGATTTCGAAGCTTTCTAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000785 GO:0000790 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0007140 GO:0007141 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009555 GO:0009987 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0034641 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044703 GO:0045132 GO:0045143 GO:0046483 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050896 GO:0051026 GO:0051276 GO:0051321 GO:0051704 GO:0051716 GO:0061982 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460

Pfam Domains

Protein Families

REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon

Protein Analysis

683

Amino Acids

76.71

Weight (kDa)

7.49

Isoelectric Point (pI)

36.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rep-A_N	PF04057	5 - 90	3.7e-24	Replication factor-A protein 1, N-terminal domain
tRNA_anti-codon	PF01336	252 - 332	2.4e-08	OB-fold nucleic acid binding domain
REPA_OB_2	PF16900	360 - 464	6.6e-28	Replication protein A OB domain
Rep_fac-A_C	PF08646	523 - 674	2.7e-50	Replication factor-A C terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012807)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G06510 AT2G06510
fragaria_vesca	FvH4_1g08261 FvH4_1g08261 FvH4_1g08261
malus_domestica	MD02G1087200.v1.1 MD15G1214800.v1.1
prunus_persica	Prupe.7G202500_v2.0.a1
pyrus_communis	pycom15g19050
rosa_chinensis	RchiOBHm_Chr2g0094351
rosa_laevigata	RLG00000016482
rosa_multiflora	Rmu_ssc0000229.1_g000002
rosa_roxburghii	Rroxscaffold_2G00147210
rosa_rugosa	Rorug02G0043400
rosa_samantha	Rh2AG091300 Rh2BG091000 Rh2CG093600 Rh2DG090000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	957
Acc36I	ACCTGC	3 cut(s)	64, 878, 961
Acc65I	GGTACC	1 cut(s)	111
AccB1I	GGYRCC	2 cut(s)	111, 697
AccII	CGCG	1 cut(s)	26
AccIII	TCCGGA	1 cut(s)	1890
AciI	CCGC	8 cut(s)	20, 26, 33, 45, 65, 253, 892, 1452
AclWI	GGATC	1 cut(s)	789
AcoI	YGGCCR	3 cut(s)	190, 267, 448
AcsI	RAATTY	5 cut(s)	619, 1194, 1475, 1715, 1755
AcuI	CTGAAG	2 cut(s)	1226, 1327
AcyI	GRCGYC	2 cut(s)	17, 698
AfaI	GTAC	5 cut(s)	113, 230, 293, 330, 1841
AfiI	CCNNNNNNNGG	5 cut(s)	121, 740, 1245, 1246, 1759
AflII	CTTAAG	1 cut(s)	1925
AgsI	TTSAA	9 cut(s)	61, 284, 688, 1014, 1072, 1748, 1849, 1906, 1921
AjnI	CCWGG	3 cut(s)	192, 479, 1483
AjuI	GAANNNNNNNTTGG	2 cut(s)	444, 476
Alw26I	GTCTC	2 cut(s)	130, 147
AlwI	GGATC	1 cut(s)	789
Ama87I	CYCGRG	2 cut(s)	804, 1403
Aor13HI	TCCGGA	1 cut(s)	1890
AoxI	GGCC	7 cut(s)	190, 267, 448, 455, 482, 676, 1581
ApeKI	GCWGC	2 cut(s)	237, 637
ApoI	RAATTY	5 cut(s)	619, 1194, 1475, 1715, 1755
ArsI	GACNNNNNNTTYG	4 cut(s)	570, 602, 1046, 1078
AseI	ATTAAT	1 cut(s)	720
Asp718I	GGTACC	1 cut(s)	111
AspLEI	GCGC	2 cut(s)	700, 958
AspS9I	GGNCC	6 cut(s)	377, 455, 483, 677, 1581, 1762
AsuHPI	GGTGA	5 cut(s)	59, 70, 1429, 1975, 2005
AsuII	TTCGAA	1 cut(s)	2036
AvaI	CYCGRG	2 cut(s)	804, 1403
AvaII	GGWCC	2 cut(s)	377, 1762
BaeGI	GKGCMC	1 cut(s)	1031
BalI	TGGCCA	1 cut(s)	450
BanI	GGYRCC	2 cut(s)	111, 697
BbvI	GCAGC	2 cut(s)	249, 649
BccI	CCATC	4 cut(s)	44, 806, 1034, 1683
BceAI	ACGGC	3 cut(s)	148, 866, 1803
BcgI	CGANNNNNNTGC	2 cut(s)	852, 886
BciT130I	CCWGG	3 cut(s)	194, 481, 1485
BciVI	GTATCC	1 cut(s)	40
BclI	TGATCA	1 cut(s)	1432
BcoDI	GTCTC	2 cut(s)	130, 147
BfaI	CTAG	2 cut(s)	179, 2046
BfmI	CTRYAG	1 cut(s)	2004
BfoI	RGCGCY	1 cut(s)	701
BfrI	CTTAAG	1 cut(s)	1925
BfuAI	ACCTGC	3 cut(s)	64, 878, 961
BfuI	GTATCC	1 cut(s)	40
BglII	AGATCT	1 cut(s)	1812
BisI	GCNGC	5 cut(s)	20, 65, 238, 638, 1452
BlpI	GCTNAGC	1 cut(s)	601
BlsI	GCNGC	5 cut(s)	21, 66, 239, 639, 1453
Bme1390I	CCNGG	3 cut(s)	194, 481, 1485
Bme18I	GGWCC	2 cut(s)	377, 1762
BmeT110I	CYCGRG	2 cut(s)	804, 1403
BmgT120I	GGNCC	6 cut(s)	377, 455, 483, 677, 1581, 1762
BmiI	GGNNCC	3 cut(s)	113, 208, 699
BmrFI	CCNGG	3 cut(s)	194, 481, 1485
BmrI	ACTGGG	3 cut(s)	603, 997, 1576
BmsI	GCATC	4 cut(s)	60, 994, 1210, 1438
BmuI	ACTGGG	3 cut(s)	603, 997, 1576
BoxI	GACNNNNGTC	1 cut(s)	1139
Bpu1102I	GCTNAGC	1 cut(s)	601
Bpu14I	TTCGAA	1 cut(s)	2036
BsaAI	YACGTR	1 cut(s)	707
BsaHI	GRCGYC	2 cut(s)	17, 698
BsaI	GGTCTC	2 cut(s)	130, 147
BsaJI	CCNNGG	4 cut(s)	153, 451, 479, 1404
BsaWI	WCCGGW	1 cut(s)	1890
Bsc4I	CCNNNNNNNGG	5 cut(s)	121, 740, 1245, 1246, 1759
Bse118I	RCCGGY	4 cut(s)	4, 114, 188, 269
Bse1I	ACTGG	9 cut(s)	336, 513, 609, 992, 1307, 1430, 1571, 1774, 1909
Bse3DI	GCAATG	1 cut(s)	395
BseAI	TCCGGA	1 cut(s)	1890
BseBI	CCWGG	3 cut(s)	194, 481, 1485
BseDI	CCNNGG	4 cut(s)	153, 451, 479, 1404
BseGI	GGATG	5 cut(s)	493, 1009, 1453, 1694, 1870
BseLI	CCNNNNNNNGG	5 cut(s)	121, 740, 1245, 1246, 1759
BseMI	GCAATG	1 cut(s)	395
BseMII	CTCAG	8 cut(s)	158, 179, 615, 837, 1089, 1154, 1199, 1538
BseNI	ACTGG	9 cut(s)	336, 513, 609, 992, 1307, 1430, 1571, 1774, 1909
BseRI	GAGGAG	4 cut(s)	822, 1503, 1736, 1952
BseSI	GKGCMC	1 cut(s)	1031
BseXI	GCAGC	2 cut(s)	249, 649
BseYI	CCCAGC	2 cut(s)	1325, 1377
BsgI	GTGCAG	1 cut(s)	656
Bsh1236I	CGCG	1 cut(s)	26
BshFI	GGCC	7 cut(s)	192, 269, 450, 457, 484, 678, 1583
BshNI	GGYRCC	2 cut(s)	111, 697
BsiHKCI	CYCGRG	2 cut(s)	804, 1403
BsiSI	CCGG	5 cut(s)	5, 115, 189, 270, 1891
BslFI	GGGAC	1 cut(s)	1436
BslI	CCNNNNNNNGG	5 cut(s)	121, 740, 1245, 1246, 1759
BsmAI	GTCTC	2 cut(s)	130, 147
BsmFI	GGGAC	1 cut(s)	1436
BsmI	GAATGC	1 cut(s)	1099
BsnI	GGCC	7 cut(s)	192, 269, 450, 457, 484, 678, 1583
Bso31I	GGTCTC	2 cut(s)	130, 147
BsoBI	CYCGRG	2 cut(s)	804, 1403
Bsp119I	TTCGAA	1 cut(s)	2036
Bsp1286I	GDGCHC	1 cut(s)	1031
Bsp13I	TCCGGA	1 cut(s)	1890
Bsp1407I	TGTACA	1 cut(s)	291
Bsp143I	GATC	8 cut(s)	49, 781, 1432, 1527, 1550, 1812, 1929, 1968
Bsp1720I	GCTNAGC	1 cut(s)	601
Bsp19I	CCATGG	1 cut(s)	153
BspACI	CCGC	8 cut(s)	20, 26, 33, 45, 65, 253, 892, 1452
BspANI	GGCC	7 cut(s)	192, 269, 450, 457, 484, 678, 1583
BspCNI	CTCAG	8 cut(s)	157, 178, 614, 838, 1090, 1155, 1198, 1537
BspEI	TCCGGA	1 cut(s)	1890
BspFNI	CGCG	1 cut(s)	26
BspLI	GGNNCC	3 cut(s)	113, 208, 699
BspMAI	CTGCAG	1 cut(s)	2008
BspMI	ACCTGC	3 cut(s)	64, 878, 961
BspPI	GGATC	1 cut(s)	789
BspT104I	TTCGAA	1 cut(s)	2036
BspT107I	GGYRCC	2 cut(s)	111, 697
BspTI	CTTAAG	1 cut(s)	1925
BspTNI	GGTCTC	2 cut(s)	130, 147
BsrDI	GCAATG	1 cut(s)	395
BsrFI	RCCGGY	4 cut(s)	4, 114, 188, 269
BsrGI	TGTACA	1 cut(s)	291
BsrI	ACTGG	9 cut(s)	336, 513, 609, 992, 1307, 1430, 1571, 1774, 1909
BssAI	RCCGGY	4 cut(s)	4, 114, 188, 269
BssECI	CCNNGG	4 cut(s)	153, 451, 479, 1404
BssMI	GATC	8 cut(s)	49, 781, 1432, 1527, 1550, 1812, 1929, 1968
BssNI	GRCGYC	2 cut(s)	17, 698
BssT1I	CCWWGG	2 cut(s)	153, 451
Bst2UI	CCWGG	3 cut(s)	194, 481, 1485
Bst4CI	ACNGT	9 cut(s)	634, 663, 1018, 1026, 1519, 1576, 1653, 1975, 1993
Bst6I	CTCTTC	1 cut(s)	1275
BstACI	GRCGYC	2 cut(s)	17, 698
BstAFI	CTTAAG	1 cut(s)	1925
BstAUI	TGTACA	1 cut(s)	291
BstBAI	YACGTR	1 cut(s)	707
BstBI	TTCGAA	1 cut(s)	2036
BstC8I	GCNNGC	5 cut(s)	24, 101, 150, 702, 1627
BstDSI	CCRYGG	1 cut(s)	153
BstEII	GGTNACC	1 cut(s)	76
BstF5I	GGATG	5 cut(s)	493, 1009, 1453, 1694, 1870
BstFNI	CGCG	1 cut(s)	26
BstH2I	RGCGCY	1 cut(s)	701
BstHHI	GCGC	2 cut(s)	700, 958
BstKTI	GATC	8 cut(s)	52, 784, 1435, 1530, 1553, 1815, 1932, 1971
BstMAI	GTCTC	2 cut(s)	130, 147
BstMBI	GATC	8 cut(s)	49, 781, 1432, 1527, 1550, 1812, 1929, 1968
BstMWI	GCNNNNNNNGC	8 cut(s)	16, 32, 70, 158, 175, 392, 758, 1322
BstNI	CCWGG	3 cut(s)	194, 481, 1485
BstNSI	RCATGY	2 cut(s)	1629, 2023
BstPAI	GACNNNNGTC	1 cut(s)	1139
BstPI	GGTNACC	1 cut(s)	76
BstSCI	CCNGG	3 cut(s)	192, 479, 1483
BstSFI	CTRYAG	1 cut(s)	2004
BstSLI	GKGCMC	1 cut(s)	1031
BstUI	CGCG	1 cut(s)	26
BstV1I	GCAGC	2 cut(s)	249, 649
BstX2I	RGATCY	1 cut(s)	1812
BstYI	RGATCY	1 cut(s)	1812
BsuI	GTATCC	1 cut(s)	40
BsuRI	GGCC	7 cut(s)	192, 269, 450, 457, 484, 678, 1583
BtgI	CCRYGG	1 cut(s)	153
BtsCI	GGATG	5 cut(s)	493, 1009, 1453, 1694, 1870
BtsI	GCAGTG	2 cut(s)	768, 1700
BtsIMutI	CAGTG	8 cut(s)	616, 768, 1023, 1351, 1572, 1658, 1700, 1980
BveI	ACCTGC	3 cut(s)	64, 878, 961
Cac8I	GCNNGC	5 cut(s)	24, 101, 150, 702, 1627
CfoI	GCGC	2 cut(s)	700, 958
Cfr10I	RCCGGY	4 cut(s)	4, 114, 188, 269
Cfr13I	GGNCC	6 cut(s)	377, 455, 483, 677, 1581, 1762
CseI	GACGC	2 cut(s)	25, 776
Csp6I	GTAC	5 cut(s)	112, 229, 292, 329, 1840
CviAII	CATG	5 cut(s)	121, 154, 1459, 1626, 2020
CviQI	GTAC	5 cut(s)	112, 229, 292, 329, 1840
DinI	GGCGCC	1 cut(s)	699
DpnI	GATC	8 cut(s)	51, 783, 1434, 1529, 1552, 1814, 1931, 1970
DpnII	GATC	8 cut(s)	49, 781, 1432, 1527, 1550, 1812, 1929, 1968
EaeI	YGGCCR	3 cut(s)	190, 267, 448
Eam1104I	CTCTTC	1 cut(s)	1275
EarI	CTCTTC	1 cut(s)	1275
EciI	GGCGGA	1 cut(s)	22
Eco130I	CCWWGG	2 cut(s)	153, 451
Eco31I	GGTCTC	2 cut(s)	130, 147
Eco32I	GATATC	3 cut(s)	85, 933, 1739
Eco47I	GGWCC	2 cut(s)	377, 1762
Eco57I	CTGAAG	2 cut(s)	1226, 1327
Eco88I	CYCGRG	2 cut(s)	804, 1403
Eco91I	GGTNACC	1 cut(s)	76
EcoO109I	RGGNCCY	2 cut(s)	455, 483
EcoO65I	GGTNACC	1 cut(s)	76
EcoRI	GAATTC	1 cut(s)	1194
EcoRII	CCWGG	3 cut(s)	192, 479, 1483
EcoRV	GATATC	3 cut(s)	85, 933, 1739
EcoT14I	CCWWGG	2 cut(s)	153, 451
EgeI	GGCGCC	1 cut(s)	699
EheI	GGCGCC	1 cut(s)	699
ErhI	CCWWGG	2 cut(s)	153, 451
FaeI	CATG	5 cut(s)	124, 157, 1462, 1629, 2023
FalI	AAGNNNNNCTT	2 cut(s)	929, 961
FaqI	GGGAC	1 cut(s)	1436
FatI	CATG	5 cut(s)	120, 153, 1458, 1625, 2019
FauNDI	CATATG	1 cut(s)	441
FbaI	TGATCA	1 cut(s)	1432
Fnu4HI	GCNGC	5 cut(s)	20, 65, 238, 638, 1452
FokI	GGATG	5 cut(s)	480, 1016, 1460, 1701, 1877
Fsp4HI	GCNGC	5 cut(s)	20, 65, 238, 638, 1452
FspBI	CTAG	2 cut(s)	179, 2046
FspI	TGCGCA	1 cut(s)	957
GlaI	GCGC	2 cut(s)	699, 957
GluI	GCNGC	5 cut(s)	20, 65, 238, 638, 1452
GsaI	CCCAGC	2 cut(s)	1329, 1381
HaeII	RGCGCY	1 cut(s)	701
HaeIII	GGCC	7 cut(s)	192, 269, 450, 457, 484, 678, 1583
HapII	CCGG	5 cut(s)	5, 115, 189, 270, 1891
HgaI	GACGC	2 cut(s)	25, 776
HhaI	GCGC	2 cut(s)	700, 958
Hin1I	GRCGYC	2 cut(s)	17, 698
Hin1II	CATG	5 cut(s)	124, 157, 1462, 1629, 2023
Hin6I	GCGC	2 cut(s)	698, 956
HinP1I	GCGC	2 cut(s)	698, 956
HincII	GTYRAC	1 cut(s)	1144
HindII	GTYRAC	1 cut(s)	1144
HindIII	AAGCTT	2 cut(s)	1922, 2039
HinfI	GANTC	9 cut(s)	335, 352, 371, 475, 571, 765, 842, 1607, 1799
HpaII	CCGG	5 cut(s)	5, 115, 189, 270, 1891
HphI	GGTGA	5 cut(s)	59, 70, 1429, 1975, 2005
Hpy166II	GTNNAC	4 cut(s)	925, 1144, 1878, 1996
Hpy8I	GTNNAC	4 cut(s)	925, 1144, 1878, 1996
Hpy99I	CGWCG	4 cut(s)	134, 215, 792, 1955
HpyAV	CCTTC	8 cut(s)	651, 738, 1158, 1201, 1259, 1324, 1532, 1954
HpyCH4III	ACNGT	9 cut(s)	634, 663, 1018, 1026, 1519, 1576, 1653, 1975, 1993
HpyCH4IV	ACGT	1 cut(s)	706
HpyF10VI	GCNNNNNNNGC	8 cut(s)	16, 32, 70, 158, 175, 392, 758, 1322
HpySE526I	ACGT	1 cut(s)	706
Hsp92I	GRCGYC	2 cut(s)	17, 698
Hsp92II	CATG	5 cut(s)	124, 157, 1462, 1629, 2023
HspAI	GCGC	2 cut(s)	698, 956
KasI	GGCGCC	1 cut(s)	697
Kpn2I	TCCGGA	1 cut(s)	1890
KpnI	GGTACC	1 cut(s)	115
Ksp22I	TGATCA	1 cut(s)	1432
Kzo9I	GATC	8 cut(s)	49, 781, 1432, 1527, 1550, 1812, 1929, 1968
LmnI	GCTCC	4 cut(s)	212, 610, 1224, 1893
Lsp1109I	GCAGC	2 cut(s)	249, 649
LweI	GCATC	4 cut(s)	60, 994, 1210, 1438
MaeI	CTAG	2 cut(s)	179, 2046
MaeII	ACGT	1 cut(s)	706
MalI	GATC	8 cut(s)	51, 783, 1434, 1529, 1552, 1814, 1931, 1970
MboI	GATC	8 cut(s)	49, 781, 1432, 1527, 1550, 1812, 1929, 1968
MboII	GAAGA	8 cut(s)	973, 1049, 1203, 1292, 1560, 1733, 1736, 1978
MfeI	CAATTG	1 cut(s)	1086
MflI	RGATCY	1 cut(s)	1812
MhlI	GDGCHC	1 cut(s)	1031
MlsI	TGGCCA	1 cut(s)	450
MluCI	AATT	9 cut(s)	409, 619, 1086, 1105, 1194, 1365, 1475, 1715, 1755
MluNI	TGGCCA	1 cut(s)	450
Mly113I	GGCGCC	1 cut(s)	698
MlyI	GAGTC	3 cut(s)	329, 380, 774
MmeI	TCCRAC	3 cut(s)	153, 404, 1663
Mox20I	TGGCCA	1 cut(s)	450
MroI	TCCGGA	1 cut(s)	1890
MscI	TGGCCA	1 cut(s)	450
Msp20I	TGGCCA	1 cut(s)	450
MspA1I	CMGCKG	2 cut(s)	67, 1060
MspCI	CTTAAG	1 cut(s)	1925
MspI	CCGG	5 cut(s)	5, 115, 189, 270, 1891
MspR9I	CCNGG	3 cut(s)	194, 481, 1485
MunI	CAATTG	1 cut(s)	1086
Mva1269I	GAATGC	1 cut(s)	1099
MvaI	CCWGG	3 cut(s)	194, 481, 1485
MvnI	CGCG	1 cut(s)	26
MwoI	GCNNNNNNNGC	8 cut(s)	16, 32, 70, 158, 175, 392, 758, 1322
NarI	GGCGCC	1 cut(s)	698
NcoI	CCATGG	1 cut(s)	153
NdeI	CATATG	1 cut(s)	441
NdeII	GATC	8 cut(s)	49, 781, 1432, 1527, 1550, 1812, 1929, 1968
NlaIII	CATG	5 cut(s)	124, 157, 1462, 1629, 2023
NlaIV	GGNNCC	3 cut(s)	113, 208, 699
NmuCI	GTSAC	7 cut(s)	76, 766, 865, 983, 1132, 1570, 1697
NsbI	TGCGCA	1 cut(s)	957
NspI	RCATGY	2 cut(s)	1629, 2023
NspV	TTCGAA	1 cut(s)	2036
PaeI	GCATGC	1 cut(s)	1629
PaeR7I	CTCGAG	1 cut(s)	804
PctI	GAATGC	1 cut(s)	1099
PfeI	GAWTC	6 cut(s)	352, 475, 571, 842, 1607, 1799
PflFI	GACNNNGTC	2 cut(s)	1568, 1886
PkrI	GCNGC	5 cut(s)	21, 66, 239, 639, 1453
PleI	GAGTC	3 cut(s)	329, 379, 773
PluTI	GGCGCC	1 cut(s)	701
PpsI	GAGTC	3 cut(s)	329, 379, 773
Ppu21I	YACGTR	1 cut(s)	707
PshAI	GACNNNNGTC	1 cut(s)	1139
PshBI	ATTAAT	1 cut(s)	720
Psp6I	CCWGG	3 cut(s)	192, 479, 1483
PspEI	GGTNACC	1 cut(s)	76
PspFI	CCCAGC	2 cut(s)	1325, 1377
PspGI	CCWGG	3 cut(s)	192, 479, 1483
PspN4I	GGNNCC	3 cut(s)	113, 208, 699
PspPI	GGNCC	6 cut(s)	377, 455, 483, 677, 1581, 1762
PspXI	VCTCGAGB	1 cut(s)	804
PstI	CTGCAG	1 cut(s)	2008
PsuI	RGATCY	1 cut(s)	1812
PsyI	GACNNNGTC	2 cut(s)	1568, 1886
PvuII	CAGCTG	1 cut(s)	1060
RsaI	GTAC	5 cut(s)	113, 230, 293, 330, 1841
RsaNI	GTAC	5 cut(s)	112, 229, 292, 329, 1840
SatI	GCNGC	5 cut(s)	20, 65, 238, 638, 1452
Sau3AI	GATC	8 cut(s)	49, 781, 1432, 1527, 1550, 1812, 1929, 1968
Sau96I	GGNCC	6 cut(s)	377, 455, 483, 677, 1581, 1762
SchI	GAGTC	3 cut(s)	329, 380, 774
ScrFI	CCNGG	3 cut(s)	194, 481, 1485
SduI	GDGCHC	1 cut(s)	1031
SfaNI	GCATC	4 cut(s)	60, 994, 1210, 1438
SfcI	CTRYAG	1 cut(s)	2004
SfoI	GGCGCC	1 cut(s)	699
Sfr274I	CTCGAG	1 cut(s)	804
SfuI	TTCGAA	1 cut(s)	2036
SinI	GGWCC	2 cut(s)	377, 1762
SlaI	CTCGAG	1 cut(s)	804
SmlI	CTYRAG	2 cut(s)	804, 1925
SmoI	CTYRAG	2 cut(s)	804, 1925
SphI	GCATGC	1 cut(s)	1629
Sse9I	AATT	9 cut(s)	409, 619, 1086, 1105, 1194, 1365, 1475, 1715, 1755
SsiI	CCGC	8 cut(s)	20, 26, 33, 45, 65, 253, 892, 1452
SspDI	GGCGCC	1 cut(s)	697
SspMI	CTAG	2 cut(s)	179, 2046
StyD4I	CCNGG	3 cut(s)	192, 479, 1483
StyI	CCWWGG	2 cut(s)	153, 451
TaaI	ACNGT	9 cut(s)	634, 663, 1018, 1026, 1519, 1576, 1653, 1975, 1993
TaiI	ACGT	1 cut(s)	709
TaqI	TCGA	4 cut(s)	805, 831, 1670, 2036
TasI	AATT	9 cut(s)	409, 619, 1086, 1105, 1194, 1365, 1475, 1715, 1755
TatI	WGTACW	4 cut(s)	228, 291, 328, 1839
TauI	GCSGC	3 cut(s)	22, 67, 1454
TfiI	GAWTC	6 cut(s)	352, 475, 571, 842, 1607, 1799
TscAI	CASTG	8 cut(s)	616, 775, 1023, 1351, 1579, 1658, 1700, 1980
TseFI	GTSAC	7 cut(s)	76, 766, 865, 983, 1132, 1570, 1697
TseI	GCWGC	2 cut(s)	237, 637
Tsp45I	GTSAC	7 cut(s)	76, 766, 865, 983, 1132, 1570, 1697
TspDTI	ATGAA	5 cut(s)	482, 1050, 1358, 1551, 1585
TspGWI	ACGGA	1 cut(s)	199
TspRI	CASTG	8 cut(s)	616, 775, 1023, 1351, 1579, 1658, 1700, 1980
Tth111I	GACNNNGTC	2 cut(s)	1568, 1886
Vha464I	CTTAAG	1 cut(s)	1925
VpaK11BI	GGWCC	2 cut(s)	377, 1762
VspI	ATTAAT	1 cut(s)	720
XapI	RAATTY	5 cut(s)	619, 1194, 1475, 1715, 1755
XceI	RCATGY	2 cut(s)	1629, 2023
XhoI	CTCGAG	1 cut(s)	804
XspI	CTAG	2 cut(s)	179, 2046

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.