Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0005636

Physical Location & Seq

Genomic Coordinates Forward (+)

3398651 .. 3405822

Gene Structure 7172 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0043400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2571 bp

ATGGCTCAGGCCAATTGGGAAGCTGATAAAATGTTGGATGTGTATATATATGATTACCTTCTGAAGAGAAATTTACATGCTTCTGCAAAGGCATTTCAAGCTGAAGGAAAAGTTTCTACAGATCCTGTAGCTATTGATGCACCCGGTGGCTTTCTTTTCGAATGGTGGTCTGTCTTCTGGGACATATTCATCGCTAGGACGAATGAAAAGCACTCTGAAGCAGCTGCATCTTATATTGAGACTCAAGTGAACAAGGCTCGGGAGCTGCAACAGAAGCCTCAGCAGCATCCGCAAATGCAAATGCAGCATCTTTTGCAAAGGCATGCTGCTCAGCAGCAGCAGCAGCAGCATCAACAACAGCAACAACAACAACACCAGCAACAACAACACCCGCAACACCACCAGCAGCAGCAGCAGCAAAGACGAGATGGGACCCAACTTCTTAATGGAACTTCCAATGGGCTCGTTGGCACTGATCCTCTTTCGAGGCAGAACTCTGCAACTGCAAATGTCATGGCAACAAAAATGTACGAGGACAGATTAAAGCCTCCCATACAGAGGGATGCTTTGGATGATGCGGCTATGAAGCAAAGGTTAGGTGACAATATGAATCAGCTTATGGATTCAAATCGTGCATCATTGGTGAAAGTAGCCACAGCAGGTGGCCAGTCTTCCGGTCAAATGCTGCATGGTACACCTGGAGGTATGTTGGGGAATCTTCAACAAGCTCACAATCGGAGTCAGCAACTTCCTGGATTGATGCAGGACATAAAGAGTGAGATGATGAACCCCAGAGCTGCTGGTCCAGAAGGATCATTGATTGGTCTTCATGGATCAAATGAAGGCAGTAGCAATTTGACTCTGAAGGGGTGGCCTTTAACGGTTCGACCTGGAATTCTTCAGCAGCAAAATTCCATGATGCAGTCCTCTCAGCCCTTTAATCAGCTTCAGCTCCAGCAACAACTTATACTTCAGGCACAACAAAATTTAGCTTCCCCATCTACCAATGACTTGGAAACTAGAAGGCTATCGATGCTCCTCAATAGAAATATACCTAACGTTGATGTACCTAACGTTGGATCGCCTGTTCAAGTGGGTTGCCCTGTAATGCCTGGTGCAGATGCAGATATGCTTATGAAGTTACAGCAACAGCAGCTGCAAAGCAACAATCAACACCAACAACAGCAACAGTATTCACAGCATCCATTTTCAAGTCAACACCCTCAGAGTTCGAATCAACACATCCAGCAGCAAGAAAAGATCACTGGTGCTGGCACCTTTACAGCTGGTGGTAGCATGTCTAACTCCTTTCAAGGCAATGATCAGGCTCCAAAGAATCAAATGGGGCGAAAGAGGAAGCAGCCAGTGTCATCTTCAGGTCCTGCCAATAGTTCAGGGACTGCTAATACCACTGGACCATCCCCCAGTTCACCTTCAACGCCTTCTACTCACACGGCAGGAGATGCGAACTCTGTGCCAACTTTGGCCCATAATAGCGGTTCATCGAAGTCTCTGCTTATGTTTGCTTCTGATGGTCTAGGCTCAGTTGCCTCGGTGCCAAATAAATTGAATGATATGGACCGATTTGTGGATGATGGATCTTTAGAGGATAATGTTGAATCATTCTTATCACATGATGATGCTGACCCTAGGGATAGAGTTGCTCGGTGTTCAGATGTCAGCAAAGGCTTCACTTTTTCGGAAGTTGGGGCTATTACTGCAAGTTCAAGTAAAGTCGAGTGCTGTCACTTCTCAGCAGATGGGAAAACACTTGCCACTGGTGGGCATGATCGAAAGGCTGTATTGTGGTCTACAGAGTCCTTCACTGTAAAGTCTACGCTTGAAGAGCATTCTCAGTGGATAACGGATGTTCGATTCAGTCCTAGTATGTCAAGGCTAGCTACATCTTCCGCTGACAAAACTGTCAGGGTCTGGGATGTTGATAATCCTGGCTATTCACTTCGTACTTTTATGGGACATTCTACAACTGTTATGTCACTTGACTTCCACCCTACTAAAGAGGATCTTCTGTGCTCTTGTGATAACAACAGTGAGATAAGATACTGGAGTATCAAGAATGGTAGTTGTATTGGAGTTTCCAAGGGTGGTGCAACTCAGATGAGGTTTCAACCTCGTCTTGGAAGGATGCTTGCTGCTGCAGGTGATACTCTTGTATCCATAATTGATGTAGAGACCGAATGTTGCACGGTTAAATTACAGGGTCATAAGAACCTTGTCAATTCTGTGTGCTGGGATTCTTCTGGTGAGTATCTAGCCTCTGTGAGTGATGACTCGGTTAGAGTATGGGCAGTTGGCTCCAGTAGCAAAGGCGAATGCCTTTACGAGTTAAGCTGTTCTGGCAATAAATTTCAGACATGCGTCTTCCATCCTACTTATCCATCATTGTTGGTAATCGGCTGTTATGAGACATTGGAACTTTGGAACATGAGCGAGAACAAGACAATGACCTTGCATGCACATGACAACCTAGTATCTTCTTTGGCAGCGTCAAGTTCTACTGGCATGGTAGCTTCAGCTAGCCATGATAAGTTCGTGAAGCTATGGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000785 GO:0000790 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0007140 GO:0007141 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009555 GO:0009987 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0034641 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044703 GO:0045132 GO:0045143 GO:0046483 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050896 GO:0051026 GO:0051276 GO:0051321 GO:0051704 GO:0051716 GO:0061982 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460

Pfam Domains

Protein Families

REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon

Protein Analysis

856

Amino Acids

93.82

Weight (kDa)

6.44

Isoelectric Point (pI)

51.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH	PF08513	10 - 36	2.4e-07	LisH
WD40_Gbeta	PF25391	563 - 699	9.4e-12	G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd	PF24782	565 - 769	4.4e-16	MABP1/WDR62 second WD40 domain
Beta-prop_THOC3	PF25174	565 - 628	7.9e-09	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	567 - 689	4.9e-13	TEP-1 second beta-propeller
EIF3I	PF24805	567 - 649	1.2e-06	EIF3I
WD40_WDHD1_1st	PF24817	568 - 649	7.1e-12	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	569 - 770	5.5e-15	WDR3 second beta-propeller domain
WD40_CDC20-Fz	PF24807	569 - 697	6.1e-16	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	572 - 695	4.1e-19	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	574 - 698	3.6e-26	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	574 - 691	1.1e-22	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	575 - 770	1.3e-31	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	576 - 683	6.2e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML	PF23409	577 - 696	4.8e-07	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	583 - 649	3.6e-07	CAF1B/HIR1 beta-propeller domain
WD40	PF00400	611 - 646	3.3e-09	WD domain, G-beta repeat
WDR55	PF24796	611 - 856	2.4e-12	WDR55
Beta-prop_THOC3	PF25174	617 - 856	1.9e-38	THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd	PF23393	629 - 735	5.9e-07	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	676 - 775	4.5e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	683 - 815	5.2e-13	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	703 - 855	1.4e-24	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	705 - 856	1.2e-13	WDHD1 first WD40 domain
WD40_Prp19	PF24814	706 - 855	5e-14	Prp19 WD40 domain
WD40_Gbeta	PF25391	707 - 855	1.3e-07	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	720 - 822	5.3e-06	CDC20/Fizzy WD40 domain
WD40	PF00400	735 - 769	1.1e-07	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	746 - 856	9.8e-08	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	765 - 855	2.4e-06	WDR3 first beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005636)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G06520
fragaria_vesca	FvH4_1g08270 FvH4_2g31210
malus_domestica	MD02G1087100.v1.1 MD08G1070100.v1.1 MD15G1214700.v1.1
prunus_persica	Prupe.1G406000_v2.0.a1 Prupe.7G202600_v2.0.a1
pyrus_communis	pycom15g04980
rosa_chinensis	RchiOBHm_Chr2g0094341 RchiOBHm_Chr6g0311601
rosa_laevigata	RLG00000010388 RLG00000016481
rosa_multiflora	Rmu_sc0002888.1_g000004 Rmu_sc0015881.1_g000006 Rmu_ssc0000229.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147220 Rroxscaffold_7G00157450
rosa_rugosa	Rorug02G0043400 Rorug06G0390000
rosa_samantha	Rh2BG091100 Rh2CG093700 Rh2DG090100 Rh6AG505200 Rh6BG516100 Rh6CG521900 Rh6DG507800
rosa_wichuraiana	Rw6G043970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	650, 2150
AasI	GACNNNNNNGTC	1 cut(s)	1922
Acc36I	ACCTGC	2 cut(s)	650, 2150
AccB1I	GGYRCC	2 cut(s)	1274, 1555
AccI	GTMKAC	2 cut(s)	1811, 1835
AciI	CCGC	5 cut(s)	290, 392, 578, 1497, 1911
AclI	AACGTT	2 cut(s)	1059, 1074
AclWI	GGATC	7 cut(s)	116, 470, 820, 841, 1087, 1606, 2031
AcoI	YGGCCR	1 cut(s)	664
AcsI	RAATTY	5 cut(s)	70, 894, 910, 985, 2366
AcuI	CTGAAG	9 cut(s)	83, 123, 237, 884, 884, 932, 956, 1359, 2517
AdeI	CACNNNGTG	1 cut(s)	146
AfaI	GTAC	4 cut(s)	530, 694, 1068, 1966
AfiI	CCNNNNNNNGG	5 cut(s)	558, 1076, 1588, 1782, 2138
AjnI	CCWGG	5 cut(s)	697, 751, 889, 1111, 1948
AloI	GAACNNNNNNTCC	2 cut(s)	1071, 1103
Alw21I	GWGCWC	1 cut(s)	2036
Alw26I	GTCTC	4 cut(s)	233, 1515, 2186, 2420
AlwI	GGATC	7 cut(s)	116, 470, 820, 841, 1087, 1606, 2031
AlwNI	CAGNNNCTG	5 cut(s)	125, 1156, 1382, 1400, 1932
Ama87I	CYCGRG	1 cut(s)	258
AoxI	GGCC	4 cut(s)	9, 664, 872, 1485
ApoI	RAATTY	5 cut(s)	70, 894, 910, 985, 2366
Asp700I	GAANNNNTTC	1 cut(s)	112
AspA2I	CCTAGG	1 cut(s)	1649
AspS9I	GGNCC	6 cut(s)	432, 803, 1379, 1415, 1486, 1579
AsuC2I	CCSGG	1 cut(s)	144
AsuHPI	GGTGA	5 cut(s)	611, 655, 1422, 2174, 2276
AsuII	TTCGAA	2 cut(s)	159, 1232
AsuNHI	GCTAGC	2 cut(s)	1897, 2537
AvaI	CYCGRG	1 cut(s)	258
AvaII	GGWCC	5 cut(s)	432, 803, 1379, 1415, 1579
AvrII	CCTAGG	1 cut(s)	1649
BaeI	ACNNNNGTAYC	8 cut(s)	2053, 2053, 2086, 2086, 2157, 2157, 2190, 2190
BalI	TGGCCA	1 cut(s)	666
BanI	GGYRCC	2 cut(s)	1274, 1555
BanII	GRGCYC	1 cut(s)	465
BbsI	GAAGAC	4 cut(s)	166, 663, 818, 2374
Bbv12I	GWGCWC	1 cut(s)	2036
BbvCI	CCTCAGC	1 cut(s)	279
BccI	CCATC	8 cut(s)	422, 1006, 1426, 1526, 1589, 1754, 2394, 2407
BceAI	ACGGC	1 cut(s)	1470
BcgI	CGANNNNNNTGC	4 cut(s)	1456, 1490, 1495, 1529
BciT130I	CCWGG	5 cut(s)	699, 753, 891, 1113, 1950
BciVI	GTATCC	1 cut(s)	2185
BclI	TGATCA	1 cut(s)	1321
BcnI	CCSGG	1 cut(s)	144
BcoDI	GTCTC	4 cut(s)	233, 1515, 2186, 2420
BfaI	CTAG	9 cut(s)	195, 1020, 1538, 1650, 1884, 1898, 2273, 2489, 2538
BfmI	CTRYAG	4 cut(s)	117, 126, 1812, 2157
BfuAI	ACCTGC	2 cut(s)	650, 2150
BfuI	GTATCC	1 cut(s)	2185
BlnI	CCTAGG	1 cut(s)	1649
BlpI	GCTNAGC	1 cut(s)	330
Bme1390I	CCNGG	6 cut(s)	144, 699, 753, 891, 1113, 1950
Bme18I	GGWCC	5 cut(s)	432, 803, 1379, 1415, 1579
BmeT110I	CYCGRG	1 cut(s)	258
BmgT120I	GGNCC	6 cut(s)	432, 803, 1379, 1415, 1486, 1579
BmiI	GGNNCC	6 cut(s)	433, 434, 1276, 1329, 1557, 2317
BmrFI	CCNGG	6 cut(s)	144, 699, 753, 891, 1113, 1950
BmrI	ACTGGG	1 cut(s)	1419
BmtI	GCTAGC	2 cut(s)	1901, 2541
BmuI	ACTGGG	1 cut(s)	1419
BoxI	GACNNNNGTC	1 cut(s)	2378
BpiI	GAAGAC	4 cut(s)	166, 663, 818, 2374
BpmI	CTGGAG	4 cut(s)	720, 938, 2086, 2302
Bpu10I	CCTNAGC	2 cut(s)	6, 279
Bpu1102I	GCTNAGC	1 cut(s)	330
Bpu14I	TTCGAA	2 cut(s)	159, 1232
BpuEI	CTTGAG	1 cut(s)	228
BpuMI	CCSGG	1 cut(s)	144
Bsa29I	ATCGAT	1 cut(s)	1031
BsaBI	GATNNNNATC	1 cut(s)	627
BsaI	GGTCTC	1 cut(s)	2186
BsaJI	CCNNGG	3 cut(s)	1551, 1649, 2100
BsaWI	WCCGGW	1 cut(s)	674
Bsc4I	CCNNNNNNNGG	5 cut(s)	558, 1076, 1588, 1782, 2138
Bse1I	ACTGG	9 cut(s)	667, 1270, 1364, 1417, 1425, 1783, 2069, 2319, 2524
Bse3DI	GCAATG	1 cut(s)	1324
Bse8I	GATNNNNATC	1 cut(s)	627
BseBI	CCWGG	5 cut(s)	699, 753, 891, 1113, 1950
BseCI	ATCGAT	1 cut(s)	1031
BseDI	CCNNGG	3 cut(s)	1551, 1649, 2100
BseJI	GATNNNNATC	1 cut(s)	627
BseLI	CCNNNNNNNGG	5 cut(s)	558, 1076, 1588, 1782, 2138
BseMI	GCAATG	1 cut(s)	1324
BseMII	CTCAG	9 cut(s)	20, 293, 344, 944, 1238, 1557, 1767, 1868, 2129
BseNI	ACTGG	9 cut(s)	667, 1270, 1364, 1417, 1425, 1783, 2069, 2319, 2524
BseRI	GAGGAG	1 cut(s)	1028
BseYI	CCCAGC	1 cut(s)	2250
BsgI	GTGCAG	1 cut(s)	1137
BshFI	GGCC	4 cut(s)	11, 666, 874, 1487
BshNI	GGYRCC	2 cut(s)	1274, 1555
BshVI	ATCGAT	1 cut(s)	1031
BsiHKAI	GWGCWC	1 cut(s)	2036
BsiHKCI	CYCGRG	1 cut(s)	258
BsiSI	CCGG	2 cut(s)	144, 675
BslFI	GGGAC	4 cut(s)	194, 445, 1411, 1989
BslI	CCNNNNNNNGG	5 cut(s)	558, 1076, 1588, 1782, 2138
BsmAI	GTCTC	4 cut(s)	233, 1515, 2186, 2420
BsmFI	GGGAC	4 cut(s)	194, 445, 1411, 1989
BsmI	GAATGC	2 cut(s)	1849, 2339
BsnI	GGCC	4 cut(s)	11, 666, 874, 1487
Bso31I	GGTCTC	1 cut(s)	2186
BsoBI	CYCGRG	1 cut(s)	258
Bsp119I	TTCGAA	2 cut(s)	159, 1232
Bsp1286I	GDGCHC	2 cut(s)	465, 2036
Bsp1720I	GCTNAGC	1 cut(s)	330
BspACI	CCGC	5 cut(s)	290, 392, 578, 1497, 1911
BspANI	GGCC	4 cut(s)	11, 666, 874, 1487
BspCNI	CTCAG	9 cut(s)	19, 292, 343, 943, 1237, 1556, 1766, 1867, 2128
BspDI	ATCGAT	1 cut(s)	1031
BspLI	GGNNCC	6 cut(s)	433, 434, 1276, 1329, 1557, 2317
BspMAI	CTGCAG	1 cut(s)	2161
BspMI	ACCTGC	2 cut(s)	650, 2150
BspOI	GCTAGC	2 cut(s)	1901, 2541
BspPI	GGATC	7 cut(s)	116, 470, 820, 841, 1087, 1606, 2031
BspQI	GCTCTTC	1 cut(s)	1839
BspT104I	TTCGAA	2 cut(s)	159, 1232
BspT107I	GGYRCC	2 cut(s)	1274, 1555
BspTNI	GGTCTC	1 cut(s)	2186
BsrDI	GCAATG	1 cut(s)	1324
BsrI	ACTGG	9 cut(s)	667, 1270, 1364, 1417, 1425, 1783, 2069, 2319, 2524
BssECI	CCNNGG	3 cut(s)	1551, 1649, 2100
BssT1I	CCWWGG	2 cut(s)	1649, 2100
Bst2UI	CCWGG	5 cut(s)	699, 753, 891, 1113, 1950
Bst4CI	ACNGT	7 cut(s)	883, 1191, 1828, 1924, 1990, 2051, 2209
Bst6I	CTCTTC	2 cut(s)	59, 1839
BstAPI	GCANNNNNTGC	2 cut(s)	313, 1463
BstBI	TTCGAA	2 cut(s)	159, 1232
BstC8I	GCNNGC	6 cut(s)	324, 1273, 1899, 2151, 2475, 2539
BstDEI	CTNAG	9 cut(s)	6, 279, 330, 930, 1224, 1543, 1753, 1854, 2115
BstMAI	GTCTC	4 cut(s)	233, 1515, 2186, 2420
BstNI	CCWGG	5 cut(s)	699, 753, 891, 1113, 1950
BstNSI	RCATGY	5 cut(s)	80, 326, 1300, 2379, 2477
BstPAI	GACNNNNGTC	1 cut(s)	2378
BstSCI	CCNGG	6 cut(s)	142, 697, 751, 889, 1111, 1948
BstSFI	CTRYAG	4 cut(s)	117, 126, 1812, 2157
BstV2I	GAAGAC	4 cut(s)	166, 663, 818, 2374
BstX2I	RGATCY	3 cut(s)	121, 1598, 2023
BstXI	CCANNNNNNTGG	1 cut(s)	1012
BstYI	RGATCY	3 cut(s)	121, 1598, 2023
Bsu15I	ATCGAT	1 cut(s)	1031
BsuI	GTATCC	1 cut(s)	2185
BsuRI	GGCC	4 cut(s)	11, 666, 874, 1487
BsuTUI	ATCGAT	1 cut(s)	1031
BtgZI	GCGATG	1 cut(s)	175
BtsIMutI	CAGTG	8 cut(s)	471, 1263, 1371, 1410, 1776, 1824, 1862, 2056
BveI	ACCTGC	2 cut(s)	650, 2150
Cac8I	GCNNGC	6 cut(s)	324, 1273, 1899, 2151, 2475, 2539
CaiI	CAGNNNCTG	5 cut(s)	125, 1156, 1382, 1400, 1932
Cfr13I	GGNCC	6 cut(s)	432, 803, 1379, 1415, 1486, 1579
ClaI	ATCGAT	1 cut(s)	1031
CseI	GACGC	2 cut(s)	2368, 2496
Csp6I	GTAC	4 cut(s)	529, 693, 1067, 1965
CviQI	GTAC	4 cut(s)	529, 693, 1067, 1965
DdeI	CTNAG	9 cut(s)	6, 279, 330, 930, 1224, 1543, 1753, 1854, 2115
DraIII	CACNNNGTG	1 cut(s)	146
DrdI	GACNNNNNNGTC	1 cut(s)	1922
DseDI	GACNNNNNNGTC	1 cut(s)	1922
EaeI	YGGCCR	1 cut(s)	664
Eam1104I	CTCTTC	2 cut(s)	59, 1839
EarI	CTCTTC	2 cut(s)	59, 1839
Eco130I	CCWWGG	2 cut(s)	1649, 2100
Eco24I	GRGCYC	1 cut(s)	465
Eco31I	GGTCTC	1 cut(s)	2186
Eco47I	GGWCC	5 cut(s)	432, 803, 1379, 1415, 1579
Eco57I	CTGAAG	9 cut(s)	83, 123, 237, 884, 884, 932, 956, 1359, 2517
Eco88I	CYCGRG	1 cut(s)	258
EcoO109I	RGGNCCY	2 cut(s)	432, 1379
EcoRI	GAATTC	1 cut(s)	894
EcoRII	CCWGG	5 cut(s)	697, 751, 889, 1111, 1948
EcoT14I	CCWWGG	2 cut(s)	1649, 2100
EcoT38I	GRGCYC	1 cut(s)	465
ErhI	CCWWGG	2 cut(s)	1649, 2100
FalI	AAGNNNNNCTT	2 cut(s)	2010, 2042
FaqI	GGGAC	4 cut(s)	194, 445, 1411, 1989
FauI	CCCGC	1 cut(s)	399
FbaI	TGATCA	1 cut(s)	1321
FblI	GTMKAC	2 cut(s)	1811, 1835
FriOI	GRGCYC	1 cut(s)	465
FspBI	CTAG	9 cut(s)	195, 1020, 1538, 1650, 1884, 1898, 2273, 2489, 2538
GsaI	CCCAGC	1 cut(s)	2254
GsuI	CTGGAG	4 cut(s)	720, 938, 2086, 2302
HaeIII	GGCC	4 cut(s)	11, 666, 874, 1487
HapII	CCGG	2 cut(s)	144, 675
HgaI	GACGC	2 cut(s)	2368, 2496
HincII	GTYRAC	1 cut(s)	1217
HindII	GTYRAC	1 cut(s)	1217
HpaII	CCGG	2 cut(s)	144, 675
HphI	GGTGA	5 cut(s)	611, 655, 1422, 2174, 2276
Hpy166II	GTNNAC	6 cut(s)	250, 695, 1217, 1430, 1812, 1836
Hpy188III	TCNNGA	4 cut(s)	260, 806, 2074, 2554
Hpy8I	GTNNAC	6 cut(s)	250, 695, 1217, 1430, 1812, 1836
HpyCH4III	ACNGT	7 cut(s)	883, 1191, 1828, 1924, 1990, 2051, 2209
HpyCH4IV	ACGT	2 cut(s)	1059, 1074
HpyF3I	CTNAG	9 cut(s)	6, 279, 330, 930, 1224, 1543, 1753, 1854, 2115
HpySE526I	ACGT	2 cut(s)	1059, 1074
KflI	GGGWCCC	1 cut(s)	432
Ksp22I	TGATCA	1 cut(s)	1321
LguI	GCTCTTC	1 cut(s)	1839
LmnI	GCTCC	5 cut(s)	262, 957, 1041, 1333, 2321
MaeI	CTAG	9 cut(s)	195, 1020, 1538, 1650, 1884, 1898, 2273, 2489, 2538
MaeII	ACGT	2 cut(s)	1059, 1074
MaeIII	GTNAC	4 cut(s)	599, 1140, 1745, 1995
MfeI	CAATTG	1 cut(s)	13
MflI	RGATCY	3 cut(s)	121, 1598, 2023
MhlI	GDGCHC	2 cut(s)	465, 2036
MlsI	TGGCCA	1 cut(s)	666
MluNI	TGGCCA	1 cut(s)	666
MlyI	GAGTC	5 cut(s)	235, 748, 853, 1826, 2285
MmeI	TCCRAC	2 cut(s)	15, 1057
Mox20I	TGGCCA	1 cut(s)	666
MroXI	GAANNNNTTC	1 cut(s)	112
MscI	TGGCCA	1 cut(s)	666
MseI	TTAA	6 cut(s)	444, 542, 878, 939, 2211, 2348
MslI	CAYNNNNRTG	2 cut(s)	1638, 2478
Msp20I	TGGCCA	1 cut(s)	666
MspA1I	CMGCKG	4 cut(s)	224, 1156, 1286, 1913
MspI	CCGG	2 cut(s)	144, 675
MspR9I	CCNGG	6 cut(s)	144, 699, 753, 891, 1113, 1950
MunI	CAATTG	1 cut(s)	13
Mva1269I	GAATGC	2 cut(s)	1849, 2339
MvaI	CCWGG	5 cut(s)	699, 753, 891, 1113, 1950
NciI	CCSGG	1 cut(s)	144
NheI	GCTAGC	2 cut(s)	1897, 2537
NlaIV	GGNNCC	6 cut(s)	433, 434, 1276, 1329, 1557, 2317
NmuCI	GTSAC	3 cut(s)	599, 1745, 1995
NspI	RCATGY	5 cut(s)	80, 326, 1300, 2379, 2477
NspV	TTCGAA	2 cut(s)	159, 1232
PaeI	GCATGC	2 cut(s)	326, 2477
PaqCI	CACCTGC	2 cut(s)	650, 2150
PciSI	GCTCTTC	1 cut(s)	1839
PctI	GAATGC	2 cut(s)	1849, 2339
PdmI	GAANNNNTTC	1 cut(s)	112
PfeI	GAWTC	8 cut(s)	610, 623, 715, 1234, 1336, 1619, 1875, 2255
PfoI	TCCNGGA	1 cut(s)	751
PleI	GAGTC	5 cut(s)	235, 747, 853, 1825, 2285
PpsI	GAGTC	5 cut(s)	235, 747, 853, 1825, 2285
PpuMI	RGGWCCY	2 cut(s)	432, 1379
PshAI	GACNNNNGTC	1 cut(s)	2378
Psp1406I	AACGTT	2 cut(s)	1059, 1074
Psp5II	RGGWCCY	2 cut(s)	432, 1379
Psp6I	CCWGG	5 cut(s)	697, 751, 889, 1111, 1948
PspFI	CCCAGC	1 cut(s)	2250
PspGI	CCWGG	5 cut(s)	697, 751, 889, 1111, 1948
PspN4I	GGNNCC	6 cut(s)	433, 434, 1276, 1329, 1557, 2317
PspPI	GGNCC	6 cut(s)	432, 803, 1379, 1415, 1486, 1579
PspPPI	RGGWCCY	2 cut(s)	432, 1379
PstI	CTGCAG	1 cut(s)	2161
PstNI	CAGNNNCTG	5 cut(s)	125, 1156, 1382, 1400, 1932
PsuI	RGATCY	3 cut(s)	121, 1598, 2023
PvuII	CAGCTG	3 cut(s)	224, 1156, 1286
RsaI	GTAC	4 cut(s)	530, 694, 1068, 1966
RsaNI	GTAC	4 cut(s)	529, 693, 1067, 1965
RseI	CAYNNNNRTG	2 cut(s)	1638, 2478
SapI	GCTCTTC	1 cut(s)	1839
SaqAI	TTAA	6 cut(s)	444, 542, 878, 939, 2211, 2348
Sau96I	GGNCC	6 cut(s)	432, 803, 1379, 1415, 1486, 1579
SchI	GAGTC	5 cut(s)	235, 748, 853, 1826, 2285
ScrFI	CCNGG	6 cut(s)	144, 699, 753, 891, 1113, 1950
SduI	GDGCHC	2 cut(s)	465, 2036
SfcI	CTRYAG	4 cut(s)	117, 126, 1812, 2157
SfuI	TTCGAA	2 cut(s)	159, 1232
SinI	GGWCC	5 cut(s)	432, 803, 1379, 1415, 1579
SmiMI	CAYNNNNRTG	2 cut(s)	1638, 2478
SmlI	CTYRAG	1 cut(s)	243
SmoI	CTYRAG	1 cut(s)	243
SphI	GCATGC	2 cut(s)	326, 2477
SsiI	CCGC	5 cut(s)	290, 392, 578, 1497, 1911
SspMI	CTAG	9 cut(s)	195, 1020, 1538, 1650, 1884, 1898, 2273, 2489, 2538
StyD4I	CCNGG	6 cut(s)	142, 697, 751, 889, 1111, 1948
StyI	CCWWGG	2 cut(s)	1649, 2100
TaaI	ACNGT	7 cut(s)	883, 1191, 1828, 1924, 1990, 2051, 2209
TaiI	ACGT	2 cut(s)	1062, 1077
TaqI	TCGA	9 cut(s)	159, 485, 886, 1031, 1232, 1505, 1737, 1792, 1873
TaqII	GACCGA	2 cut(s)	1596, 2210
TauI	GCSGC	1 cut(s)	581
TfiI	GAWTC	8 cut(s)	610, 623, 715, 1234, 1336, 1619, 1875, 2255
Tru1I	TTAA	6 cut(s)	444, 542, 878, 939, 2211, 2348
Tru9I	TTAA	6 cut(s)	444, 542, 878, 939, 2211, 2348
TscAI	CASTG	8 cut(s)	478, 1270, 1371, 1417, 1783, 1831, 1862, 2056
TseFI	GTSAC	3 cut(s)	599, 1745, 1995
Tsp45I	GTSAC	3 cut(s)	599, 1745, 1995
TspDTI	ATGAA	9 cut(s)	178, 219, 599, 623, 800, 818, 855, 1151, 1491
TspGWI	ACGGA	1 cut(s)	1880
TspRI	CASTG	8 cut(s)	478, 1270, 1371, 1417, 1783, 1831, 1862, 2056
VpaK11BI	GGWCC	5 cut(s)	432, 803, 1379, 1415, 1579
XapI	RAATTY	5 cut(s)	70, 894, 910, 985, 2366
XceI	RCATGY	5 cut(s)	80, 326, 1300, 2379, 2477
XcmI	CCANNNNNNNNNTGG	1 cut(s)	443
XmaJI	CCTAGG	1 cut(s)	1649
XmiI	GTMKAC	2 cut(s)	1811, 1835
XmnI	GAANNNNTTC	1 cut(s)	112
XspI	CTAG	9 cut(s)	195, 1020, 1538, 1650, 1884, 1898, 2273, 2489, 2538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.