FvH4_2g31210

Photosystem II reaction centre X protein (PsbX)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005636
Physical Location & Seq
Reverse (-)
23987004 .. 23987372
369 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31210.t1

Sequence Viewer

Length: 369 bp
ATGGCTTCTGTTTCTATGGCTATGCCAATTATGTCTGCCACTCAAAACAGAACAAGCCAGCCAAGTTCATTGTTGAAGTCTATAATCCCAGTTAGGCAGCAAAACAGGGCTGTGTCTGTGAAGCCAAAGTCTTCCACAGACATGCTCAAAGTGCAGGCCTCTTTGAAAGAGAAGGCAGTCACAGGCCTCGCTGCAGCTGCATTGACAGCTTCCATGGTGATACCTGAGGTGGCTGAGGCGGCCGGGGCCGGGGTAACCCCATCTCTCAACAACTTTTTGCTTAGCATTGCAGCTGGAGGAGTTGTGCTTGTAGGAATTCTTGGAGCTGTTATTGGTGTATCCAACTTTGATCCAGTTAAGAGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PsbX

Protein Analysis

123

Amino Acids

12.4

Weight (kDa)

10.37

Isoelectric Point (pI)

30.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbX PF06596 85 - 121 2.2e-16 Photosystem II reaction centre X protein (PsbX)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 239
AclWI GGATC 1 cut(s) 344
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 1 cut(s) 315
AfiI CCNNNNNNNGG 1 cut(s) 249
AgsI TTSAA 2 cut(s) 76, 166
AluBI AGCT 4 cut(s) 197, 209, 293, 326
AluI AGCT 4 cut(s) 197, 209, 293, 326
AlwI GGATC 1 cut(s) 344
AoxI GGCC 4 cut(s) 156, 184, 240, 246
ApeKI GCWGC 5 cut(s) 97, 191, 194, 197, 290
ApoI RAATTY 1 cut(s) 315
AspS9I GGNCC 1 cut(s) 246
AsuC2I CCSGG 2 cut(s) 244, 250
AsuHPI GGTGA 1 cut(s) 229
AxyI CCTNAGG 1 cut(s) 225
BbsI GAAGAC 1 cut(s) 123
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 5 cut(s) 109, 178, 184, 206, 302
BccI CCATC 1 cut(s) 268
BciVI GTATCC 1 cut(s) 349
BcnI CCSGG 2 cut(s) 244, 250
BfmI CTRYAG 1 cut(s) 192
BfuI GTATCC 1 cut(s) 349
BisI GCNGC 6 cut(s) 98, 192, 195, 198, 240, 291
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 6 cut(s) 99, 193, 196, 199, 241, 292
Bme1390I CCNGG 2 cut(s) 244, 250
BmgT120I GGNCC 1 cut(s) 246
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 2 cut(s) 244, 250
BmrI ACTGGG 1 cut(s) 83
BmuI ACTGGG 1 cut(s) 83
BpiI GAAGAC 1 cut(s) 123
BpmI CTGGAG 1 cut(s) 315
Bpu10I CCTNAGC 1 cut(s) 234
Bpu1102I GCTNAGC 1 cut(s) 281
BpuMI CCSGG 2 cut(s) 244, 250
BsaJI CCNNGG 3 cut(s) 213, 243, 249
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 1 cut(s) 249
Bse1I ACTGG 2 cut(s) 89, 353
Bse21I CCTNAGG 1 cut(s) 225
Bse3DI GCAATG 1 cut(s) 285
BseDI CCNNGG 3 cut(s) 213, 243, 249
BseLI CCNNNNNNNGG 1 cut(s) 249
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 2 cut(s) 216, 225
BseNI ACTGG 2 cut(s) 89, 353
BseRI GAGGAG 1 cut(s) 312
BseX3I CGGCCG 1 cut(s) 240
BseXI GCAGC 5 cut(s) 109, 178, 184, 206, 302
BsgI GTGCAG 1 cut(s) 173
Bsh1285I CGRYCG 1 cut(s) 243
BshFI GGCC 4 cut(s) 158, 186, 242, 248
BsiEI CGRYCG 1 cut(s) 243
BsiSI CCGG 2 cut(s) 243, 249
BslI CCNNNNNNNGG 1 cut(s) 249
BsnI GGCC 4 cut(s) 158, 186, 242, 248
Bsp143I GATC 1 cut(s) 349
Bsp1720I GCTNAGC 1 cut(s) 281
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 1 cut(s) 239
BspANI GGCC 4 cut(s) 158, 186, 242, 248
BspCNI CTCAG 2 cut(s) 217, 226
BspLI GGNNCC 1 cut(s) 247
BspMAI CTGCAG 1 cut(s) 196
BspPI GGATC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 2 cut(s) 89, 353
BssECI CCNNGG 3 cut(s) 213, 243, 249
BssMI GATC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 213
BstC8I GCNNGC 2 cut(s) 59, 156
BstDEI CTNAG 3 cut(s) 225, 234, 281
BstDSI CCRYGG 1 cut(s) 213
BstEII GGTNACC 1 cut(s) 253
BstKTI GATC 1 cut(s) 352
BstMBI GATC 1 cut(s) 349
BstMCI CGRYCG 1 cut(s) 243
BstMWI GCNNNNNNNGC 5 cut(s) 151, 197, 206, 239, 245
BstNSI RCATGY 1 cut(s) 145
BstPI GGTNACC 1 cut(s) 253
BstSCI CCNGG 2 cut(s) 242, 248
BstSFI CTRYAG 1 cut(s) 192
BstV1I GCAGC 5 cut(s) 109, 178, 184, 206, 302
BstV2I GAAGAC 1 cut(s) 123
BstZI CGGCCG 1 cut(s) 240
Bsu36I CCTNAGG 1 cut(s) 225
BsuI GTATCC 1 cut(s) 349
BsuRI GGCC 4 cut(s) 158, 186, 242, 248
BtgI CCRYGG 1 cut(s) 213
Cac8I GCNNGC 2 cut(s) 59, 156
Cfr13I GGNCC 1 cut(s) 246
CviAII CATG 2 cut(s) 142, 214
DdeI CTNAG 3 cut(s) 225, 234, 281
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 240
EagI CGGCCG 1 cut(s) 240
EclXI CGGCCG 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 213
Eco147I AGGCCT 2 cut(s) 158, 186
Eco52I CGGCCG 1 cut(s) 240
Eco81I CCTNAGG 1 cut(s) 225
Eco91I GGTNACC 1 cut(s) 253
EcoO65I GGTNACC 1 cut(s) 253
EcoRI GAATTC 1 cut(s) 315
EcoT14I CCWWGG 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 2 cut(s) 145, 217
FaiI YATR 6 cut(s) 17, 23, 32, 83, 143, 215
FatI CATG 2 cut(s) 141, 213
Fnu4HI GCNGC 6 cut(s) 98, 192, 195, 198, 240, 291
Fsp4HI GCNGC 6 cut(s) 98, 192, 195, 198, 240, 291
GluI GCNGC 6 cut(s) 98, 192, 195, 198, 240, 291
GsuI CTGGAG 1 cut(s) 315
HaeIII GGCC 4 cut(s) 158, 186, 242, 248
HapII CCGG 2 cut(s) 243, 249
Hin1II CATG 2 cut(s) 145, 217
HpaII CCGG 2 cut(s) 243, 249
HphI GGTGA 1 cut(s) 229
HpyAV CCTTC 1 cut(s) 166
HpyCH4V TGCA 4 cut(s) 154, 194, 200, 290
HpyF10VI GCNNNNNNNGC 5 cut(s) 151, 197, 206, 239, 245
HpyF3I CTNAG 3 cut(s) 225, 234, 281
Hsp92II CATG 2 cut(s) 145, 217
Kzo9I GATC 1 cut(s) 349
LmnI GCTCC 1 cut(s) 323
LpnPI CCDG 9 cut(s) 71, 91, 102, 140, 168, 237, 256, 262, 279
Lsp1109I GCAGC 5 cut(s) 109, 178, 184, 206, 302
MaeIII GTNAC 2 cut(s) 178, 253
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MboII GAAGA 1 cut(s) 123
MluCI AATT 2 cut(s) 27, 315
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 5 cut(s) 169, 197, 220, 229, 290
MseI TTAA 2 cut(s) 357, 367
MslI CAYNNNNRTG 1 cut(s) 140
MspA1I CMGCKG 2 cut(s) 197, 293
MspI CCGG 2 cut(s) 243, 249
MspR9I CCNGG 2 cut(s) 244, 250
MwoI GCNNNNNNNGC 5 cut(s) 151, 197, 206, 239, 245
NciI CCSGG 2 cut(s) 244, 250
NcoI CCATGG 1 cut(s) 213
NdeII GATC 1 cut(s) 349
NlaIII CATG 2 cut(s) 145, 217
NlaIV GGNNCC 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 178
NspI RCATGY 1 cut(s) 145
PceI AGGCCT 2 cut(s) 158, 186
PkrI GCNGC 6 cut(s) 99, 193, 196, 199, 241, 292
PspEI GGTNACC 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 1 cut(s) 246
PstI CTGCAG 1 cut(s) 196
PvuII CAGCTG 2 cut(s) 197, 293
RseI CAYNNNNRTG 1 cut(s) 140
SaqAI TTAA 2 cut(s) 357, 367
SatI GCNGC 6 cut(s) 98, 192, 195, 198, 240, 291
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 1 cut(s) 246
ScrFI CCNGG 2 cut(s) 244, 250
SetI ASST 6 cut(s) 199, 211, 226, 231, 295, 328
SfcI CTRYAG 1 cut(s) 192
SmiMI CAYNNNNRTG 1 cut(s) 140
Sse9I AATT 2 cut(s) 27, 315
SseBI AGGCCT 2 cut(s) 158, 186
SsiI CCGC 1 cut(s) 239
StuI AGGCCT 2 cut(s) 158, 186
StyD4I CCNGG 2 cut(s) 242, 248
StyI CCWWGG 1 cut(s) 213
TasI AATT 2 cut(s) 27, 315
TauI GCSGC 1 cut(s) 242
Tru1I TTAA 2 cut(s) 357, 367
Tru9I TTAA 2 cut(s) 357, 367
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 5 cut(s) 97, 191, 194, 197, 290
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 1 cut(s) 57
XapI RAATTY 1 cut(s) 315
XceI RCATGY 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.