MD15G1214700.v1.1

Photosystem II reaction centre X protein (PsbX)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005636
Physical Location & Seq
Forward (+)
17248884 .. 17249630
747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1214700.v1.1.491

Sequence Viewer

Length: 378 bp
ATGGCATCAACCACAGCAGTTACAATGGCCATGCCATTGACCAATGCAACCCAAAAGAGGATCCAGTCCTCCGCTGGGTGCTTCTTCAAGCCACTGCCACTGCCACTGAGGCCTTCAAAGGCACTGGCACCCGCGGCGTCAAAATCGAGCGGGAGGCTCCTAGTCAGGGCTTCGTTGAAGGAGAAGGCGGTGACTGGAATCACGGCGGCCGCGCTCACCGCCTCAATGGTGATCCCGGAGGTGGCCGAAGCGGCCTCAGGAGTTTCTCCTTCCCTGAAGAACTTCTTGCTCAGTATTGCGGCCGGCGGCGTTGTAGCTGTTGTGATCATCGGTGCTGTAGTTGGTGTGTCCAATTTCGACCCTGTTAAGAGAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PsbX

Protein Analysis

126

Amino Acids

12.55

Weight (kDa)

10.74

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbX PF06596 88 - 124 4.6e-15 Photosystem II reaction centre X protein (PsbX)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 127
AccBSI CCGCTC 1 cut(s) 150
AccII CGCG 2 cut(s) 134, 212
AclWI GGATC 3 cut(s) 55, 68, 226
AcoI YGGCCR 4 cut(s) 27, 207, 243, 300
AcuI CTGAAG 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 137
AfiI CCNNNNNNNGG 4 cut(s) 57, 75, 166, 241
AgsI TTSAA 3 cut(s) 88, 117, 178
AluBI AGCT 1 cut(s) 317
AluI AGCT 1 cut(s) 317
AlwI GGATC 3 cut(s) 55, 68, 226
AoxI GGCC 6 cut(s) 27, 110, 207, 243, 252, 300
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 1 cut(s) 214
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 3 cut(s) 202, 208, 241
AxyI CCTNAGG 1 cut(s) 256
BalI TGGCCA 1 cut(s) 29
BamHI GGATCC 1 cut(s) 60
BanI GGYRCC 1 cut(s) 127
BceAI ACGGC 1 cut(s) 219
BclI TGATCA 1 cut(s) 324
BcnI CCSGG 1 cut(s) 236
BfaI CTAG 1 cut(s) 161
BfmI CTRYAG 1 cut(s) 336
BglI GCCNNNNNGGC 2 cut(s) 109, 251
BisI GCNGC 6 cut(s) 135, 207, 210, 252, 300, 307
BlsI GCNGC 6 cut(s) 136, 208, 211, 253, 301, 308
Bme1390I CCNGG 1 cut(s) 236
BmiI GGNNCC 3 cut(s) 62, 129, 158
BmrFI CCNGG 1 cut(s) 236
BmsI GCATC 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 236
BsaHI GRCGYC 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 132
Bsc4I CCNNNNNNNGG 4 cut(s) 57, 75, 166, 241
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 3 cut(s) 64, 129, 199
Bse21I CCTNAGG 1 cut(s) 256
BseDI CCNNGG 1 cut(s) 132
BseLI CCNNNNNNNGG 4 cut(s) 57, 75, 166, 241
BseMII CTCAG 3 cut(s) 98, 270, 304
BseNI ACTGG 3 cut(s) 64, 129, 199
BseX3I CGGCCG 2 cut(s) 207, 300
BseYI CCCAGC 1 cut(s) 74
Bsh1236I CGCG 2 cut(s) 134, 212
Bsh1285I CGRYCG 2 cut(s) 210, 303
BshFI GGCC 6 cut(s) 29, 112, 209, 245, 254, 302
BshNI GGYRCC 1 cut(s) 127
BsiEI CGRYCG 2 cut(s) 210, 303
BsiSI CCGG 2 cut(s) 236, 303
BslI CCNNNNNNNGG 4 cut(s) 57, 75, 166, 241
BsnI GGCC 6 cut(s) 29, 112, 209, 245, 254, 302
Bsp143I GATC 3 cut(s) 60, 231, 324
BspANI GGCC 6 cut(s) 29, 112, 209, 245, 254, 302
BspCNI CTCAG 3 cut(s) 99, 269, 303
BspFNI CGCG 2 cut(s) 134, 212
BspLI GGNNCC 3 cut(s) 62, 129, 158
BspPI GGATC 3 cut(s) 55, 68, 226
BspT107I GGYRCC 1 cut(s) 127
BsrBI CCGCTC 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 3 cut(s) 64, 129, 199
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 3 cut(s) 60, 231, 324
BssNI GRCGYC 1 cut(s) 137
BstACI GRCGYC 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 3 cut(s) 107, 256, 290
BstDSI CCRYGG 1 cut(s) 132
BstFNI CGCG 2 cut(s) 134, 212
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 3 cut(s) 63, 234, 327
BstMBI GATC 3 cut(s) 60, 231, 324
BstMCI CGRYCG 2 cut(s) 210, 303
BstMWI GCNNNNNNNGC 4 cut(s) 109, 134, 218, 251
BstSCI CCNGG 1 cut(s) 234
BstSFI CTRYAG 1 cut(s) 336
BstUI CGCG 2 cut(s) 134, 212
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BstZI CGGCCG 2 cut(s) 207, 300
Bsu36I CCTNAGG 1 cut(s) 256
BsuRI GGCC 6 cut(s) 29, 112, 209, 245, 254, 302
BtgI CCRYGG 1 cut(s) 132
BtsI GCAGTG 2 cut(s) 92, 98
BtsIMutI CAGTG 4 cut(s) 92, 98, 104, 122
Cac8I GCNNGC 1 cut(s) 304
CciNI GCGGCCGC 1 cut(s) 207
CfoI GCGC 1 cut(s) 214
Cfr10I RCCGGY 1 cut(s) 302
Cfr42I CCGCGG 1 cut(s) 135
CseI GACGC 1 cut(s) 126
CviAII CATG 1 cut(s) 31
DdeI CTNAG 3 cut(s) 107, 256, 290
DpnI GATC 3 cut(s) 62, 233, 326
DpnII GATC 3 cut(s) 60, 231, 324
EaeI YGGCCR 4 cut(s) 27, 207, 243, 300
EagI CGGCCG 2 cut(s) 207, 300
EclXI CGGCCG 2 cut(s) 207, 300
Eco147I AGGCCT 1 cut(s) 112
Eco52I CGGCCG 2 cut(s) 207, 300
Eco57I CTGAAG 1 cut(s) 296
Eco81I CCTNAGG 1 cut(s) 256
FaeI CATG 1 cut(s) 34
FaiI YATR 1 cut(s) 32
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FatI CATG 1 cut(s) 30
FauI CCCGC 2 cut(s) 139, 143
FbaI TGATCA 1 cut(s) 324
Fnu4HI GCNGC 6 cut(s) 135, 207, 210, 252, 300, 307
Fsp4HI GCNGC 6 cut(s) 135, 207, 210, 252, 300, 307
FspBI CTAG 1 cut(s) 161
GlaI GCGC 1 cut(s) 213
GluI GCNGC 6 cut(s) 135, 207, 210, 252, 300, 307
GsaI CCCAGC 1 cut(s) 78
HaeIII GGCC 6 cut(s) 29, 112, 209, 245, 254, 302
HapII CCGG 2 cut(s) 236, 303
HgaI GACGC 1 cut(s) 126
HhaI GCGC 1 cut(s) 214
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 1 cut(s) 34
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HinfI GANTC 1 cut(s) 198
HpaII CCGG 2 cut(s) 236, 303
HphI GGTGA 3 cut(s) 202, 208, 241
Hpy188III TCNNGA 1 cut(s) 258
HpyAV CCTTC 4 cut(s) 123, 172, 178, 279
HpyCH4V TGCA 1 cut(s) 47
HpyF10VI GCNNNNNNNGC 4 cut(s) 109, 134, 218, 251
HpyF3I CTNAG 3 cut(s) 107, 256, 290
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 1 cut(s) 34
HspAI GCGC 1 cut(s) 212
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Ksp22I TGATCA 1 cut(s) 324
KspI CCGCGG 1 cut(s) 135
Kzo9I GATC 3 cut(s) 60, 231, 324
LmnI GCTCC 1 cut(s) 162
LpnPI CCDG 9 cut(s) 60, 77, 110, 151, 180, 243, 249, 287, 316
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 161
MaeIII GTNAC 2 cut(s) 19, 190
MalI GATC 3 cut(s) 62, 233, 326
MbiI CCGCTC 1 cut(s) 150
MboI GATC 3 cut(s) 60, 231, 324
MboII GAAGA 2 cut(s) 76, 289
MflI RGATCY 1 cut(s) 60
MlsI TGGCCA 1 cut(s) 29
MluCI AATT 1 cut(s) 352
MluNI TGGCCA 1 cut(s) 29
MnlI CCTC 8 cut(s) 51, 79, 102, 147, 232, 232, 265, 365
Mox20I TGGCCA 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 302
MroXI GAANNNNTTC 1 cut(s) 281
MscI TGGCCA 1 cut(s) 29
MseI TTAA 1 cut(s) 366
Msp20I TGGCCA 1 cut(s) 29
MspA1I CMGCKG 2 cut(s) 74, 134
MspI CCGG 2 cut(s) 236, 303
MspR9I CCNGG 1 cut(s) 236
MvnI CGCG 2 cut(s) 134, 212
MwoI GCNNNNNNNGC 4 cut(s) 109, 134, 218, 251
NaeI GCCGGC 1 cut(s) 304
NciI CCSGG 1 cut(s) 236
NdeII GATC 3 cut(s) 60, 231, 324
NgoMIV GCCGGC 1 cut(s) 302
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 3 cut(s) 62, 129, 158
NmuCI GTSAC 1 cut(s) 190
NotI GCGGCCGC 1 cut(s) 207
PceI AGGCCT 1 cut(s) 112
PdiI GCCGGC 1 cut(s) 304
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 1 cut(s) 198
PfoI TCCNGGA 1 cut(s) 234
PkrI GCNGC 6 cut(s) 136, 208, 211, 253, 301, 308
PspFI CCCAGC 1 cut(s) 74
PspN4I GGNNCC 3 cut(s) 62, 129, 158
PsuI RGATCY 1 cut(s) 60
SacII CCGCGG 1 cut(s) 135
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 6 cut(s) 135, 207, 210, 252, 300, 307
Sau3AI GATC 3 cut(s) 60, 231, 324
ScrFI CCNGG 1 cut(s) 236
SetI ASST 2 cut(s) 243, 319
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 1 cut(s) 336
SfiI GGCCNNNNNGGCC 1 cut(s) 251
Sfr303I CCGCGG 1 cut(s) 135
SgrBI CCGCGG 1 cut(s) 135
Sse9I AATT 1 cut(s) 352
SseBI AGGCCT 1 cut(s) 112
SspMI CTAG 1 cut(s) 161
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 1 cut(s) 234
TaqI TCGA 2 cut(s) 146, 357
TasI AATT 1 cut(s) 352
TauI GCSGC 6 cut(s) 137, 209, 212, 254, 302, 309
TfiI GAWTC 1 cut(s) 198
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 4 cut(s) 99, 105, 111, 129
TseFI GTSAC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 190
TspRI CASTG 4 cut(s) 99, 105, 111, 129
XcmI CCANNNNNNNNNTGG 1 cut(s) 71
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.