Rroxscaffold_3G00229010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0017857
Physical Location & Seq
Forward (+)
13548501 .. 13563545
15045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00229010.1

Sequence Viewer

Length: 441 bp
ATGTTTGGCTCCAATTTTGTTGCAGCTGGTCAACGGTCTCTTTTCTTGCGCGGGCGTGCTAGCACCGTCGGGTGCGGTCGTCGGGGGTGTCCCTTGACCCGACTTCTTTCAAGAGATTGTGGACGAGGAGAGCACCAACCTCGTCGTGGGATTCTTTGTGCCTCGTGGCGAGGACTTTTGCCGAGCTTCTTGAAAATCACAATCTATACTCGATGTTGTAGATCAAGCGAGCGAGAACCACTAGGAAATAAGGAGAACTTGCTAAAGCGTGACTTTAGCTTAGCCGGGTTGCTAGGGCGTTACCCTTGCTTGGCCGGTTCTGTAACGGTTGTGGTCGCGGCACTACCGTCGGCTCCCGAGGAGACTAGGACCGAAGTATGTTGGCAGAGGGTTTGGTGGCACCGAAAGTCGGCTTCGAGAAGACTAGGACTAGGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

16.23

Weight (kDa)

10.95

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 399
AccII CGCG 2 cut(s) 51, 338
AciI CCGC 3 cut(s) 51, 75, 338
AcoI YGGCCR 1 cut(s) 312
AfiI CCNNNNNNNGG 3 cut(s) 146, 310, 409
AgsI TTSAA 2 cut(s) 111, 193
AluBI AGCT 3 cut(s) 26, 186, 279
AluI AGCT 3 cut(s) 26, 186, 279
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 2 cut(s) 42, 356
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 1 cut(s) 312
ApeKI GCWGC 1 cut(s) 23
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 1 cut(s) 369
AsuC2I CCSGG 1 cut(s) 286
AsuNHI GCTAGC 1 cut(s) 59
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 1 cut(s) 369
BanI GGYRCC 1 cut(s) 399
BauI CACGAG 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 35
BcgI CGANNNNNNTGC 4 cut(s) 122, 156, 330, 364
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 2 cut(s) 42, 356
BfaI CTAG 6 cut(s) 60, 242, 293, 366, 425, 431
BisI GCNGC 2 cut(s) 24, 339
BlpI GCTNAGC 1 cut(s) 280
BlsI GCNGC 2 cut(s) 25, 340
Bme1390I CCNGG 1 cut(s) 286
Bme18I GGWCC 1 cut(s) 369
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 369
BmiI GGNNCC 3 cut(s) 10, 354, 401
BmrFI CCNGG 1 cut(s) 286
BmtI GCTAGC 1 cut(s) 63
BpiI GAAGAC 1 cut(s) 427
Bpu1102I GCTNAGC 1 cut(s) 280
BpuMI CCSGG 1 cut(s) 286
BsaI GGTCTC 1 cut(s) 42
BsaJI CCNNGG 1 cut(s) 357
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 310, 409
Bse118I RCCGGY 1 cut(s) 314
BseDI CCNNGG 1 cut(s) 357
BseLI CCNNNNNNNGG 3 cut(s) 146, 310, 409
BseRI GAGGAG 2 cut(s) 141, 374
BseXI GCAGC 1 cut(s) 35
Bsh1236I CGCG 2 cut(s) 51, 338
Bsh1285I CGRYCG 1 cut(s) 79
BshFI GGCC 1 cut(s) 314
BshNI GGYRCC 1 cut(s) 399
BsiEI CGRYCG 1 cut(s) 79
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 2 cut(s) 285, 315
BslFI GGGAC 1 cut(s) 75
BslI CCNNNNNNNGG 3 cut(s) 146, 310, 409
BsmAI GTCTC 2 cut(s) 42, 356
BsmFI GGGAC 1 cut(s) 75
BsnI GGCC 1 cut(s) 314
Bso31I GGTCTC 1 cut(s) 42
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 1 cut(s) 221
Bsp1720I GCTNAGC 1 cut(s) 280
BspACI CCGC 3 cut(s) 51, 75, 338
BspANI GGCC 1 cut(s) 314
BspFNI CGCG 2 cut(s) 51, 338
BspLI GGNNCC 3 cut(s) 10, 354, 401
BspOI GCTAGC 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 399
BspTNI GGTCTC 1 cut(s) 42
BsrFI RCCGGY 1 cut(s) 314
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 1 cut(s) 357
BssMI GATC 1 cut(s) 221
BssSI CACGAG 1 cut(s) 163
Bst2BI CACGAG 1 cut(s) 163
Bst4CI ACNGT 4 cut(s) 36, 67, 328, 348
BstC8I GCNNGC 4 cut(s) 53, 57, 61, 230
BstDEI CTNAG 1 cut(s) 280
BstFNI CGCG 2 cut(s) 51, 338
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 1 cut(s) 224
BstMAI GTCTC 2 cut(s) 42, 356
BstMBI GATC 1 cut(s) 221
BstMCI CGRYCG 1 cut(s) 79
BstSCI CCNGG 1 cut(s) 284
BstUI CGCG 2 cut(s) 51, 338
BstV1I GCAGC 1 cut(s) 35
BstV2I GAAGAC 1 cut(s) 427
BsuRI GGCC 1 cut(s) 314
Cac8I GCNNGC 4 cut(s) 53, 57, 61, 230
CfoI GCGC 1 cut(s) 51
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 369
CviJI RGCY 8 cut(s) 9, 26, 186, 279, 284, 314, 353, 413
CviKI_1 RGCY 8 cut(s) 9, 26, 186, 279, 284, 314, 353, 413
DdeI CTNAG 1 cut(s) 280
DpnI GATC 1 cut(s) 223
DpnII GATC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 312
Eco31I GGTCTC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 369
Eco88I CYCGRG 1 cut(s) 356
FaiI YATR 2 cut(s) 207, 379
FalI AAGNNNNNCTT 4 cut(s) 242, 274, 257, 289
FaqI GGGAC 1 cut(s) 75
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 2 cut(s) 24, 339
Fsp4HI GCNGC 2 cut(s) 24, 339
FspBI CTAG 6 cut(s) 60, 242, 293, 366, 425, 431
GlaI GCGC 1 cut(s) 50
GluI GCNGC 2 cut(s) 24, 339
HaeIII GGCC 1 cut(s) 314
HapII CCGG 2 cut(s) 285, 315
HhaI GCGC 1 cut(s) 51
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HincII GTYRAC 1 cut(s) 32
HindII GTYRAC 1 cut(s) 32
HinfI GANTC 1 cut(s) 151
HpaII CCGG 2 cut(s) 285, 315
Hpy166II GTNNAC 2 cut(s) 32, 122
Hpy188III TCNNGA 4 cut(s) 111, 190, 356, 417
Hpy8I GTNNAC 2 cut(s) 32, 122
Hpy99I CGWCG 4 cut(s) 71, 84, 147, 352
HpyCH4III ACNGT 4 cut(s) 36, 67, 328, 348
HpyCH4V TGCA 1 cut(s) 23
HpyF3I CTNAG 1 cut(s) 280
HspAI GCGC 1 cut(s) 49
Kzo9I GATC 1 cut(s) 221
LmnI GCTCC 2 cut(s) 14, 358
LpnPI CCDG 3 cut(s) 12, 298, 328
Lsp1109I GCAGC 1 cut(s) 35
MaeI CTAG 6 cut(s) 60, 242, 293, 366, 425, 431
MaeIII GTNAC 3 cut(s) 269, 299, 322
MalI GATC 1 cut(s) 223
MboI GATC 1 cut(s) 221
MboII GAAGA 1 cut(s) 432
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 1 cut(s) 13
MnlI CCTC 6 cut(s) 119, 150, 164, 172, 352, 381
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 2 cut(s) 285, 315
MspR9I CCNGG 1 cut(s) 286
MvnI CGCG 2 cut(s) 51, 338
NciI CCSGG 1 cut(s) 286
NdeII GATC 1 cut(s) 221
NheI GCTAGC 1 cut(s) 59
NlaIV GGNNCC 3 cut(s) 10, 354, 401
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 1 cut(s) 269
PfeI GAWTC 1 cut(s) 151
PkrI GCNGC 2 cut(s) 25, 340
PspN4I GGNNCC 3 cut(s) 10, 354, 401
PspPI GGNCC 1 cut(s) 369
PvuII CAGCTG 1 cut(s) 26
SatI GCNGC 2 cut(s) 24, 339
Sau3AI GATC 1 cut(s) 221
Sau96I GGNCC 1 cut(s) 369
ScrFI CCNGG 1 cut(s) 286
SduI GDGCHC 1 cut(s) 135
SetI ASST 4 cut(s) 28, 142, 188, 281
SinI GGWCC 1 cut(s) 369
Sse9I AATT 1 cut(s) 13
SsiI CCGC 3 cut(s) 51, 75, 338
SspMI CTAG 6 cut(s) 60, 242, 293, 366, 425, 431
StyD4I CCNGG 1 cut(s) 284
TaaI ACNGT 4 cut(s) 36, 67, 328, 348
TaqI TCGA 2 cut(s) 211, 416
TaqII GACCGA 1 cut(s) 386
TasI AATT 1 cut(s) 13
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 1 cut(s) 151
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 1 cut(s) 23
Tsp45I GTSAC 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XspI CTAG 6 cut(s) 60, 242, 293, 366, 425, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.