Rroxscaffold_7G00179100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017857
Physical Location & Seq
Reverse (-)
18667013 .. 18678819
11807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00179100.1

Sequence Viewer

Length: 885 bp
ATGATCCTTGGGAAAAACCACTCCTTCGACGCCGGTTTGGTGGCCGGAGATGGCCGGAATCGCAGGAAATCGATGAAAATCTCAAACTACTACGTAAACTTCTCGCCTTGGTTCGCCGTTTTCCGGCCAAAACTCGACAATCCATCACCACGTGCGCGTCCAGAGGAGGAGACGAGTCTATTGACACCAACCTTGCACCACCAGTGGCCGGAAAAGAAGAGGAGGCCGGAGCTACAGATCGCCGAAGGAGAGAGGAAAGCGCGGGGAGGAGGAGAGAGAGAGGAAAAGAAAGTTACCGGCCCCCAACAAGTACAGAAGCGAGCCAAGAAACAAAAGTTCTTTCTCGTTTTATTTGACGTTAGAGCTACAAGGCTTCATATTCCCATTGAACTGAAGCAGAATGCAGAAAAAGATCACTGCATTTCAAGTAGCAACATCCTTCACACTGACCATGTTGAAAATCCCACGTGCCATATCAATTTGACCACAAAGAGAAACGCGCCTCACGTGCCTAAGTTTTCACGAGATTCTTCTTTCTACGTCCGATTGTCGCCGTTCATCGACGAAGCCATGGGGCTCGTAGTGGCCATCTTGGCTAAAGACTTCAAGAAGAGAGGCCTGGCTTGGGTTGGGAAATTCTGGATAGATCGCATCGGCAGATCCGATCGGAGGTCGTCGAAATCGGTGGTGGATTGGTTCGTCCGCATCAGATCGGATGGTGATCGAGCAGAGCGAAAATCACAGGGAAGTGGGGAGAACTTGCTAAAGAGTGACTTTAGCTTGGCTGGTTTGCAAGGGCGTTACCCTTGCTTGGCTGGTTCCGTAACCGTTGTGGTCGCTATACTACGGTCGGCTCTAGAGGAGACTAGGACCGAAGCACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

294

Amino Acids

33.5

Weight (kDa)

10.11

Isoelectric Point (pI)

58.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 157, 262, 500
AciI CCGC 2 cut(s) 262, 703
AclWI GGATC 1 cut(s) 654
AcoI YGGCCR 5 cut(s) 42, 52, 125, 206, 585
AcsI RAATTY 1 cut(s) 635
AcuI CTGAAG 1 cut(s) 413
AcvI CACGTG 3 cut(s) 152, 468, 508
AcyI GRCGYC 1 cut(s) 30
AdeI CACNNNGTG 1 cut(s) 152
AfaI GTAC 1 cut(s) 312
AfiI CCNNNNNNNGG 5 cut(s) 123, 208, 625, 669, 811
AgsI TTSAA 4 cut(s) 389, 426, 458, 607
AjnI CCWGG 1 cut(s) 618
AluBI AGCT 3 cut(s) 232, 365, 780
AluI AGCT 3 cut(s) 232, 365, 780
Alw26I GTCTC 2 cut(s) 164, 857
AlwI GGATC 1 cut(s) 654
AoxI GGCC 8 cut(s) 42, 52, 125, 206, 224, 298, 585, 616
ApoI RAATTY 1 cut(s) 635
AspLEI GCGC 3 cut(s) 157, 262, 502
AspS9I GGNCC 2 cut(s) 299, 870
AsuHPI GGTGA 2 cut(s) 138, 731
AvaII GGWCC 1 cut(s) 870
BalI TGGCCA 1 cut(s) 587
BanII GRGCYC 1 cut(s) 579
BauI CACGAG 1 cut(s) 522
BbrPI CACGTG 3 cut(s) 152, 468, 508
BccI CCATC 4 cut(s) 44, 151, 596, 710
BceAI ACGGC 2 cut(s) 101, 538
BciT130I CCWGG 1 cut(s) 620
BcoDI GTCTC 2 cut(s) 164, 857
BfaI CTAG 2 cut(s) 857, 867
BfmI CTRYAG 1 cut(s) 233
BglI GCCNNNNNGGC 1 cut(s) 593
Bme1390I CCNGG 1 cut(s) 620
Bme18I GGWCC 1 cut(s) 870
BmgT120I GGNCC 2 cut(s) 299, 870
BmiI GGNNCC 2 cut(s) 301, 820
BmrFI CCNGG 1 cut(s) 620
BmsI GCATC 2 cut(s) 660, 714
Bsa29I ATCGAT 1 cut(s) 71
BsaAI YACGTR 4 cut(s) 94, 152, 468, 508
BsaBI GATNNNNATC 2 cut(s) 77, 720
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 3 cut(s) 7, 107, 570
Bsc4I CCNNNNNNNGG 5 cut(s) 123, 208, 625, 669, 811
Bse118I RCCGGY 2 cut(s) 32, 296
Bse1I ACTGG 1 cut(s) 202
Bse8I GATNNNNATC 2 cut(s) 77, 720
BseBI CCWGG 1 cut(s) 620
BseCI ATCGAT 1 cut(s) 71
BseDI CCNNGG 3 cut(s) 7, 107, 570
BseGI GGATG 2 cut(s) 435, 721
BseJI GATNNNNATC 2 cut(s) 77, 720
BseLI CCNNNNNNNGG 5 cut(s) 123, 208, 625, 669, 811
BseNI ACTGG 1 cut(s) 202
BseRI GAGGAG 6 cut(s) 179, 182, 235, 282, 285, 875
Bsh1236I CGCG 3 cut(s) 157, 262, 500
Bsh1285I CGRYCG 2 cut(s) 667, 851
BshFI GGCC 8 cut(s) 44, 54, 127, 208, 226, 300, 587, 618
BshVI ATCGAT 1 cut(s) 71
BsiEI CGRYCG 2 cut(s) 667, 851
BsiSI CCGG 7 cut(s) 33, 45, 55, 124, 209, 227, 297
BslI CCNNNNNNNGG 5 cut(s) 123, 208, 625, 669, 811
BsmAI GTCTC 2 cut(s) 164, 857
BsmBI CGTCTC 1 cut(s) 164
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 8 cut(s) 44, 54, 127, 208, 226, 300, 587, 618
Bsp1286I GDGCHC 1 cut(s) 579
Bsp143I GATC 8 cut(s) 3, 237, 412, 646, 659, 664, 710, 721
Bsp19I CCATGG 1 cut(s) 570
BspACI CCGC 2 cut(s) 262, 703
BspANI GGCC 8 cut(s) 44, 54, 127, 208, 226, 300, 587, 618
BspDI ATCGAT 1 cut(s) 71
BspFNI CGCG 3 cut(s) 157, 262, 500
BspLI GGNNCC 2 cut(s) 301, 820
BspPI GGATC 1 cut(s) 654
BsrFI RCCGGY 2 cut(s) 32, 296
BsrI ACTGG 1 cut(s) 202
BssAI RCCGGY 2 cut(s) 32, 296
BssECI CCNNGG 3 cut(s) 7, 107, 570
BssMI GATC 8 cut(s) 3, 237, 412, 646, 659, 664, 710, 721
BssNI GRCGYC 1 cut(s) 30
BssSI CACGAG 1 cut(s) 522
BssT1I CCWWGG 3 cut(s) 7, 107, 570
Bst2BI CACGAG 1 cut(s) 522
Bst2UI CCWGG 1 cut(s) 620
Bst4CI ACNGT 2 cut(s) 829, 849
Bst6I CTCTTC 2 cut(s) 212, 605
BstACI GRCGYC 1 cut(s) 30
BstBAI YACGTR 4 cut(s) 94, 152, 468, 508
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 1 cut(s) 513
BstDSI CCRYGG 1 cut(s) 570
BstF5I GGATG 2 cut(s) 435, 721
BstFNI CGCG 3 cut(s) 157, 262, 500
BstHHI GCGC 3 cut(s) 157, 262, 502
BstKTI GATC 8 cut(s) 6, 240, 415, 649, 662, 667, 713, 724
BstMAI GTCTC 2 cut(s) 164, 857
BstMBI GATC 8 cut(s) 3, 237, 412, 646, 659, 664, 710, 721
BstMCI CGRYCG 2 cut(s) 667, 851
BstMWI GCNNNNNNNGC 3 cut(s) 60, 508, 593
BstNI CCWGG 1 cut(s) 620
BstSCI CCNGG 1 cut(s) 618
BstSFI CTRYAG 1 cut(s) 233
BstSNI TACGTA 1 cut(s) 94
BstUI CGCG 3 cut(s) 157, 262, 500
BstX2I RGATCY 1 cut(s) 659
BstYI RGATCY 1 cut(s) 659
Bsu15I ATCGAT 1 cut(s) 71
BsuRI GGCC 8 cut(s) 44, 54, 127, 208, 226, 300, 587, 618
BsuTUI ATCGAT 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 570
BtsCI GGATG 2 cut(s) 435, 721
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 3 cut(s) 209, 415, 444
Cac8I GCNNGC 1 cut(s) 321
CfoI GCGC 3 cut(s) 157, 262, 502
Cfr10I RCCGGY 2 cut(s) 32, 296
Cfr13I GGNCC 2 cut(s) 299, 870
ClaI ATCGAT 1 cut(s) 71
CseI GACGC 2 cut(s) 38, 146
Csp6I GTAC 1 cut(s) 311
CviAII CATG 2 cut(s) 452, 571
CviQI GTAC 1 cut(s) 311
DdeI CTNAG 1 cut(s) 513
DpnI GATC 8 cut(s) 5, 239, 414, 648, 661, 666, 712, 723
DpnII GATC 8 cut(s) 3, 237, 412, 646, 659, 664, 710, 721
DraIII CACNNNGTG 1 cut(s) 152
EaeI YGGCCR 5 cut(s) 42, 52, 125, 206, 585
Eam1104I CTCTTC 2 cut(s) 212, 605
EarI CTCTTC 2 cut(s) 212, 605
Eco105I TACGTA 1 cut(s) 94
Eco130I CCWWGG 3 cut(s) 7, 107, 570
Eco147I AGGCCT 1 cut(s) 618
Eco24I GRGCYC 1 cut(s) 579
Eco47I GGWCC 1 cut(s) 870
Eco57I CTGAAG 1 cut(s) 413
Eco72I CACGTG 3 cut(s) 152, 468, 508
EcoRII CCWGG 1 cut(s) 618
EcoT14I CCWWGG 3 cut(s) 7, 107, 570
EcoT38I GRGCYC 1 cut(s) 579
ErhI CCWWGG 3 cut(s) 7, 107, 570
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 2 cut(s) 455, 574
FaiI YATR 5 cut(s) 378, 453, 474, 572, 842
FalI AAGNNNNNCTT 2 cut(s) 758, 790
FatI CATG 2 cut(s) 451, 570
FauI CCCGC 1 cut(s) 255
FokI GGATG 2 cut(s) 422, 728
FriOI GRGCYC 1 cut(s) 579
FspBI CTAG 2 cut(s) 857, 867
GlaI GCGC 3 cut(s) 156, 261, 501
HaeIII GGCC 8 cut(s) 44, 54, 127, 208, 226, 300, 587, 618
HapII CCGG 7 cut(s) 33, 45, 55, 124, 209, 227, 297
HgaI GACGC 2 cut(s) 38, 146
HhaI GCGC 3 cut(s) 157, 262, 502
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 2 cut(s) 455, 574
Hin6I GCGC 3 cut(s) 155, 260, 500
HinP1I GCGC 3 cut(s) 155, 260, 500
HinfI GANTC 3 cut(s) 58, 175, 527
HpaII CCGG 7 cut(s) 33, 45, 55, 124, 209, 227, 297
HphI GGTGA 2 cut(s) 138, 731
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 5 cut(s) 545, 664, 669, 710, 715
Hpy188III TCNNGA 5 cut(s) 161, 522, 607, 640, 857
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 3 cut(s) 32, 566, 679
HpyAV CCTTC 3 cut(s) 34, 239, 449
HpyCH4III ACNGT 2 cut(s) 829, 849
HpyCH4IV ACGT 7 cut(s) 93, 151, 357, 467, 507, 540, 880
HpyCH4V TGCA 4 cut(s) 196, 404, 420, 793
HpyF10VI GCNNNNNNNGC 3 cut(s) 60, 508, 593
HpyF3I CTNAG 1 cut(s) 513
HpySE526I ACGT 7 cut(s) 93, 151, 357, 467, 507, 540, 880
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 2 cut(s) 455, 574
HspAI GCGC 3 cut(s) 155, 260, 500
Kzo9I GATC 8 cut(s) 3, 237, 412, 646, 659, 664, 710, 721
LmnI GCTCC 1 cut(s) 229
LweI GCATC 2 cut(s) 660, 714
MaeI CTAG 2 cut(s) 857, 867
MaeII ACGT 7 cut(s) 93, 151, 357, 467, 507, 540, 880
MaeIII GTNAC 4 cut(s) 292, 770, 800, 823
MalI GATC 8 cut(s) 5, 239, 414, 648, 661, 666, 712, 723
MboI GATC 8 cut(s) 3, 237, 412, 646, 659, 664, 710, 721
MboII GAAGA 3 cut(s) 229, 522, 622
MflI RGATCY 1 cut(s) 659
MhlI GDGCHC 1 cut(s) 579
MlsI TGGCCA 1 cut(s) 587
MluCI AATT 2 cut(s) 478, 635
MluNI TGGCCA 1 cut(s) 587
MlyI GAGTC 1 cut(s) 184
Mox20I TGGCCA 1 cut(s) 587
MscI TGGCCA 1 cut(s) 587
Msp20I TGGCCA 1 cut(s) 587
MspI CCGG 7 cut(s) 33, 45, 55, 124, 209, 227, 297
MspR9I CCNGG 1 cut(s) 620
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 1 cut(s) 620
MvnI CGCG 3 cut(s) 157, 262, 500
MwoI GCNNNNNNNGC 3 cut(s) 60, 508, 593
NcoI CCATGG 1 cut(s) 570
NdeII GATC 8 cut(s) 3, 237, 412, 646, 659, 664, 710, 721
NlaIII CATG 2 cut(s) 455, 574
NlaIV GGNNCC 2 cut(s) 301, 820
NmuCI GTSAC 1 cut(s) 770
PceI AGGCCT 1 cut(s) 618
PcsI WCGNNNNNNNCGW 3 cut(s) 504, 660, 730
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 2 cut(s) 58, 527
Ple19I CGATCG 1 cut(s) 667
PleI GAGTC 1 cut(s) 183
PmaCI CACGTG 3 cut(s) 152, 468, 508
PmlI CACGTG 3 cut(s) 152, 468, 508
PpsI GAGTC 1 cut(s) 183
Ppu21I YACGTR 4 cut(s) 94, 152, 468, 508
Psp6I CCWGG 1 cut(s) 618
PspCI CACGTG 3 cut(s) 152, 468, 508
PspGI CCWGG 1 cut(s) 618
PspN4I GGNNCC 2 cut(s) 301, 820
PspPI GGNCC 2 cut(s) 299, 870
PsuI RGATCY 1 cut(s) 659
PvuI CGATCG 1 cut(s) 667
RsaI GTAC 1 cut(s) 312
RsaNI GTAC 1 cut(s) 311
Sau3AI GATC 8 cut(s) 3, 237, 412, 646, 659, 664, 710, 721
Sau96I GGNCC 2 cut(s) 299, 870
SchI GAGTC 1 cut(s) 184
ScrFI CCNGG 1 cut(s) 620
SduI GDGCHC 1 cut(s) 579
SfaNI GCATC 2 cut(s) 660, 714
SfcI CTRYAG 1 cut(s) 233
SinI GGWCC 1 cut(s) 870
SnaBI TACGTA 1 cut(s) 94
Sse9I AATT 2 cut(s) 478, 635
SseBI AGGCCT 1 cut(s) 618
SsiI CCGC 2 cut(s) 262, 703
SspMI CTAG 2 cut(s) 857, 867
StuI AGGCCT 1 cut(s) 618
StyD4I CCNGG 1 cut(s) 618
StyI CCWWGG 3 cut(s) 7, 107, 570
TaaI ACNGT 2 cut(s) 829, 849
TaiI ACGT 7 cut(s) 96, 154, 360, 470, 510, 543, 883
TaqI TCGA 6 cut(s) 27, 71, 135, 561, 677, 724
TasI AATT 2 cut(s) 478, 635
TatI WGTACW 1 cut(s) 310
TfiI GAWTC 2 cut(s) 58, 527
TscAI CASTG 3 cut(s) 209, 422, 451
TseFI GTSAC 1 cut(s) 770
Tsp45I GTSAC 1 cut(s) 770
TspDTI ATGAA 3 cut(s) 89, 365, 547
TspGWI ACGGA 1 cut(s) 811
TspRI CASTG 3 cut(s) 209, 422, 451
VpaK11BI GGWCC 1 cut(s) 870
XapI RAATTY 1 cut(s) 635
XbaI TCTAGA 1 cut(s) 856
XspI CTAG 2 cut(s) 857, 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.