Rroxscaffold_3G00244380

CASP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014450
Physical Location & Seq
Reverse (-)
37062810 .. 37064744
1935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00244380.1

Sequence Viewer

Length: 573 bp
ATGTCAACGGTGGTAGAGAAGTCTGCAGCGGAGGTTGCACCGCGAGACGAGTTAGAAGGGATGAGTAGCAACGGCAACGGCAGCAGCTTGAGAACGGCGGAGACGCTGCTGCGACTGCTGCCGATTGCTCCTTGTGTTGCGGCTCTGCTTGTCATGCTTCAGGATTCCCAGGACAATGAGTTCGGCTCCGTTTCCTACTCTCATATTGGAGCTTTCAGGTTTTTGGTGCATGCCAATGGCATATGTGCTGGTTATTCCCTTCTCTCGGGCTATCATATCAGCCAAGTCTCGTTCCCCCACCAATTGCTTACGTACATAATTCTGGGAGCTGGAGCTGTGTCAACGGAGGTTCTGTACTTGGCTTATAAAGGAGATTCAACTGTAACGTGGAGTCCGGCTTGTGGGAGTTACGGTGGATTCTGTCACAAAGCCACAACTTCTGTGGTCATTACATTTCTGGTGGTGGCTTGCTTTGTTCTCATTTCACTTATTTCCTCCTACAAACTCTTCAGCAAGTTTGATGCTCCTGCCATCAACTCCATTAAGGGCATTCGAACTGCCACCTTCGGTTGA

Protein Analysis

190

Amino Acids

20.16

Weight (kDa)

6.49

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 28 - 157 1.5e-28 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17200 AT1G17200
fragaria_vesca FvH4_5g24950
malus_domestica MD06G1011400.v1.1
prunus_persica Prupe.5G013700_v2.0.a1
pyrus_communis pycom06g00940
rosa_chinensis RchiOBHm_Chr7g0215251
rosa_laevigata RLG00000002677
rosa_multiflora Rmu_sc0002701.1_g000034 Rmu_sc0007904.1_g000026
rosa_roxburghii Rroxscaffold_3G00244380
rosa_rugosa Rorug07G0151500
rosa_samantha Rh7AG288200 Rh7BG280300 Rh7CG308000 Rh7DG291800
rosa_wichuraiana Rw7G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
AccII CGCG 1 cut(s) 43
AciI CCGC 4 cut(s) 29, 41, 98, 140
AcuI CTGAAG 2 cut(s) 143, 493
AfaI GTAC 2 cut(s) 314, 356
AfiI CCNNNNNNNGG 2 cut(s) 265, 401
AgsI TTSAA 1 cut(s) 378
AjnI CCWGG 1 cut(s) 168
AloI GAACNNNNNNTCC 2 cut(s) 164, 196
AluBI AGCT 4 cut(s) 87, 212, 329, 335
AluI AGCT 4 cut(s) 87, 212, 329, 335
Alw26I GTCTC 3 cut(s) 39, 95, 292
Ama87I CYCGRG 1 cut(s) 265
ApeKI GCWGC 6 cut(s) 26, 81, 84, 106, 109, 118
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
AsuII TTCGAA 1 cut(s) 553
AvaI CYCGRG 1 cut(s) 265
BbvI GCAGC 6 cut(s) 38, 93, 93, 96, 96, 105
BccI CCATC 1 cut(s) 539
BceAI ACGGC 3 cut(s) 88, 94, 111
BciT130I CCWGG 1 cut(s) 170
BcoDI GTCTC 3 cut(s) 39, 95, 292
BfmI CTRYAG 1 cut(s) 24
BisI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
BlsI GCNGC 7 cut(s) 28, 83, 86, 108, 111, 120, 142
Bme1390I CCNGG 1 cut(s) 170
BmeT110I CYCGRG 1 cut(s) 265
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 170
BmsI GCATC 1 cut(s) 511
BplI GAGNNNNNCTC 2 cut(s) 170, 202
BpmI CTGGAG 1 cut(s) 351
Bpu14I TTCGAA 1 cut(s) 553
BpuEI CTTGAG 1 cut(s) 109
BsaAI YACGTR 1 cut(s) 312
BsaJI CCNNGG 1 cut(s) 168
BsaXI ACNNNNNCTCC 4 cut(s) 338, 368, 397, 427
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 401
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 66
BseLI CCNNNNNNNGG 2 cut(s) 265, 401
BseXI GCAGC 6 cut(s) 38, 93, 93, 96, 96, 105
Bsh1236I CGCG 1 cut(s) 43
BsiHKCI CYCGRG 1 cut(s) 265
BsiSI CCGG 1 cut(s) 395
BslI CCNNNNNNNGG 2 cut(s) 265, 401
BsmAI GTCTC 3 cut(s) 39, 95, 292
BsmBI CGTCTC 2 cut(s) 39, 95
BsmI GAATGC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 265
Bsp119I TTCGAA 1 cut(s) 553
BspACI CCGC 4 cut(s) 29, 41, 98, 140
BspFNI CGCG 1 cut(s) 43
BspLI GGNNCC 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 28
BspT104I TTCGAA 1 cut(s) 553
BssECI CCNNGG 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 3 cut(s) 10, 382, 413
Bst6I CTCTTC 1 cut(s) 512
BstBAI YACGTR 1 cut(s) 312
BstBI TTCGAA 1 cut(s) 553
BstC8I GCNNGC 2 cut(s) 231, 469
BstF5I GGATG 1 cut(s) 66
BstFNI CGCG 1 cut(s) 43
BstMAI GTCTC 3 cut(s) 39, 95, 292
BstMWI GCNNNNNNNGC 5 cut(s) 35, 81, 115, 118, 154
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 233
BstSCI CCNGG 1 cut(s) 168
BstSFI CTRYAG 1 cut(s) 24
BstSNI TACGTA 1 cut(s) 312
BstUI CGCG 1 cut(s) 43
BstV1I GCAGC 6 cut(s) 38, 93, 93, 96, 96, 105
BtsCI GGATG 1 cut(s) 66
Cac8I GCNNGC 2 cut(s) 231, 469
CseI GACGC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 313, 355
CspCI CAANNNNNGTGG 2 cut(s) 423, 458
CviAII CATG 2 cut(s) 154, 230
CviQI GTAC 2 cut(s) 313, 355
Eam1104I CTCTTC 1 cut(s) 512
EarI CTCTTC 1 cut(s) 512
EciI GGCGGA 1 cut(s) 113
Eco105I TACGTA 1 cut(s) 312
Eco57I CTGAAG 2 cut(s) 143, 493
Eco88I CYCGRG 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 168
Esp3I CGTCTC 2 cut(s) 39, 95
FaeI CATG 2 cut(s) 157, 233
FaiI YATR 8 cut(s) 155, 204, 231, 242, 244, 276, 317, 366
FatI CATG 2 cut(s) 153, 229
FauNDI CATATG 1 cut(s) 242
Fnu4HI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
FokI GGATG 1 cut(s) 73
Fsp4HI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
GluI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
GsuI CTGGAG 1 cut(s) 351
HapII CCGG 1 cut(s) 395
HgaI GACGC 1 cut(s) 112
Hin1II CATG 2 cut(s) 157, 233
HincII GTYRAC 2 cut(s) 6, 342
HindII GTYRAC 2 cut(s) 6, 342
HinfI GANTC 4 cut(s) 164, 374, 391, 417
HpaII CCGG 1 cut(s) 395
Hpy166II GTNNAC 2 cut(s) 6, 342
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 2 cut(s) 6, 342
HpyAV CCTTC 2 cut(s) 50, 269
HpyCH4III ACNGT 3 cut(s) 10, 382, 413
HpyCH4IV ACGT 2 cut(s) 311, 386
HpyCH4V TGCA 3 cut(s) 26, 38, 229
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 81, 115, 118, 154
HpySE526I ACGT 2 cut(s) 311, 386
Hsp92II CATG 2 cut(s) 157, 233
LmnI GCTCC 6 cut(s) 133, 191, 209, 326, 332, 529
Lsp1109I GCAGC 6 cut(s) 38, 93, 93, 96, 96, 105
LweI GCATC 1 cut(s) 511
MaeII ACGT 2 cut(s) 311, 386
MaeIII GTNAC 3 cut(s) 382, 407, 422
MboII GAAGA 1 cut(s) 499
MfeI CAATTG 1 cut(s) 302
MluCI AATT 2 cut(s) 302, 318
MlyI GAGTC 1 cut(s) 400
MnlI CCTC 3 cut(s) 25, 340, 505
MseI TTAA 1 cut(s) 543
MslI CAYNNNNRTG 1 cut(s) 234
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 1 cut(s) 170
MunI CAATTG 1 cut(s) 302
Mva1269I GAATGC 1 cut(s) 549
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 43
MwoI GCNNNNNNNGC 5 cut(s) 35, 81, 115, 118, 154
NdeI CATATG 1 cut(s) 242
NlaIII CATG 2 cut(s) 157, 233
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 422
NspI RCATGY 1 cut(s) 233
NspV TTCGAA 1 cut(s) 553
PaeI GCATGC 1 cut(s) 233
PctI GAATGC 1 cut(s) 549
PfeI GAWTC 3 cut(s) 164, 374, 417
PkrI GCNGC 7 cut(s) 28, 83, 86, 108, 111, 120, 142
PleI GAGTC 1 cut(s) 399
PpsI GAGTC 1 cut(s) 399
Ppu21I YACGTR 1 cut(s) 312
PsiI TTATAA 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 187
PstI CTGCAG 1 cut(s) 28
RsaI GTAC 2 cut(s) 314, 356
RsaNI GTAC 2 cut(s) 313, 355
RseI CAYNNNNRTG 1 cut(s) 234
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 1 cut(s) 170
SfaNI GCATC 1 cut(s) 511
SfcI CTRYAG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 553
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
SnaBI TACGTA 1 cut(s) 312
SphI GCATGC 1 cut(s) 233
Sse9I AATT 2 cut(s) 302, 318
SsiI CCGC 4 cut(s) 29, 41, 98, 140
StyD4I CCNGG 1 cut(s) 168
TaaI ACNGT 3 cut(s) 10, 382, 413
TaiI ACGT 2 cut(s) 314, 389
TaqI TCGA 1 cut(s) 553
TasI AATT 2 cut(s) 302, 318
TatI WGTACW 1 cut(s) 354
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 3 cut(s) 164, 374, 417
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 6 cut(s) 26, 81, 84, 106, 109, 118
Tsp45I GTSAC 1 cut(s) 422
TspGWI ACGGA 2 cut(s) 178, 359
XceI RCATGY 1 cut(s) 233
XcmI CCANNNNNNNNNTGG 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.