Rh7BG280300

CASP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0014450
Physical Location & Seq
Forward (+)
26084502 .. 26087058
2557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG280300.1

Sequence Viewer

Length: 606 bp
ATGTCAACGGTGATAGAGAAGTCTGCAGCGGAGGTTGCACCGGGAGACGAGTTAGAAGGGATGAGTAGCAACGGCAACGGCAGCAGCTTGAGAACGGCGGAGACGCTGCTGCGACTGCTGCCGATTGCTCCTTGTGTGGCGGCTCTGCTCGTCATGCTTCACGATTCCCAGGACAATGAGTTCGGATCCGTTTCCTACTCTCATATTGGAGCTTTCAGGTTTTTGGTGCATGCCAATGGCATATGTGCTGGTTATTCCCTTCTATCGGCTATCATATCAGCCAAGTCTCGTCCCCCCACCAAGTCTCTAGCCTGGACTTTCTTCTTCATAGATCAATTGCTTACGTACATAATTCTGGGAGCTGGAGCTGTGTCAACGGAGGTTCTGTACTTGGCTTATAAAGGAGATTCAACTATAACGTGGAGTCCGGCTTGTGGGAGTTATGGTGGATTCTGTCACAAAGCCACAACTTCTGTGGTCATTACATTTCTGGTGGTGGCTTGCTTTGTTCTCATTTCACTTATTTCCTCCTACAAACTCTTCAGCAAGTTTGATGCTCCTGCCATCAACTCCATTAAGGGCATTCGAACTGCCACCTTCGGTTGA

Protein Analysis

201

Amino Acids

21.32

Weight (kDa)

6.88

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 28 - 168 9.1e-40 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17200 AT1G17200
fragaria_vesca FvH4_5g24950
malus_domestica MD06G1011400.v1.1
prunus_persica Prupe.5G013700_v2.0.a1
pyrus_communis pycom06g00940
rosa_chinensis RchiOBHm_Chr7g0215251
rosa_laevigata RLG00000002677
rosa_multiflora Rmu_sc0002701.1_g000034 Rmu_sc0007904.1_g000026
rosa_roxburghii Rroxscaffold_3G00244380
rosa_rugosa Rorug07G0151500
rosa_samantha Rh7AG288200 Rh7BG280300 Rh7CG308000 Rh7DG291800
rosa_wichuraiana Rw7G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 399
AciI CCGC 3 cut(s) 29, 98, 140
AclWI GGATC 2 cut(s) 180, 193
AcuI CTGAAG 1 cut(s) 526
AfaI GTAC 2 cut(s) 347, 389
AfiI CCNNNNNNNGG 2 cut(s) 265, 434
AgsI TTSAA 1 cut(s) 411
AjnI CCWGG 2 cut(s) 168, 311
AloI GAACNNNNNNTCC 2 cut(s) 164, 196
AluBI AGCT 4 cut(s) 87, 212, 362, 368
AluI AGCT 4 cut(s) 87, 212, 362, 368
Alw26I GTCTC 4 cut(s) 39, 95, 291, 309
AlwI GGATC 2 cut(s) 180, 193
ApeKI GCWGC 6 cut(s) 26, 81, 84, 106, 109, 118
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
AsuC2I CCSGG 1 cut(s) 42
AsuHPI GGTGA 1 cut(s) 22
AsuII TTCGAA 1 cut(s) 586
BamHI GGATCC 1 cut(s) 185
BbvI GCAGC 6 cut(s) 38, 93, 93, 96, 96, 105
BccI CCATC 1 cut(s) 572
BceAI ACGGC 3 cut(s) 88, 94, 111
BciT130I CCWGG 2 cut(s) 170, 313
BcnI CCSGG 1 cut(s) 42
BcoDI GTCTC 4 cut(s) 39, 95, 291, 309
BfaI CTAG 1 cut(s) 308
BfmI CTRYAG 1 cut(s) 24
BisI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
BlsI GCNGC 7 cut(s) 28, 83, 86, 108, 111, 120, 142
Bme1390I CCNGG 3 cut(s) 42, 170, 313
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 3 cut(s) 42, 170, 313
BmsI GCATC 1 cut(s) 544
BpmI CTGGAG 1 cut(s) 384
Bpu14I TTCGAA 1 cut(s) 586
BpuEI CTTGAG 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 42
BsaAI YACGTR 1 cut(s) 345
BsaJI CCNNGG 1 cut(s) 168
BsaXI ACNNNNNCTCC 4 cut(s) 371, 401, 430, 460
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 434
BseBI CCWGG 2 cut(s) 170, 313
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 66
BseLI CCNNNNNNNGG 2 cut(s) 265, 434
BseXI GCAGC 6 cut(s) 38, 93, 93, 96, 96, 105
BsiSI CCGG 2 cut(s) 41, 428
BslFI GGGAC 1 cut(s) 276
BslI CCNNNNNNNGG 2 cut(s) 265, 434
BsmAI GTCTC 4 cut(s) 39, 95, 291, 309
BsmBI CGTCTC 2 cut(s) 39, 95
BsmFI GGGAC 1 cut(s) 276
BsmI GAATGC 1 cut(s) 582
Bsp119I TTCGAA 1 cut(s) 586
Bsp143I GATC 2 cut(s) 185, 331
BspACI CCGC 3 cut(s) 29, 98, 140
BspLI GGNNCC 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 28
BspPI GGATC 2 cut(s) 180, 193
BspT104I TTCGAA 1 cut(s) 586
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 2 cut(s) 185, 331
Bst2UI CCWGG 2 cut(s) 170, 313
Bst4CI ACNGT 1 cut(s) 10
Bst6I CTCTTC 1 cut(s) 545
BstBAI YACGTR 1 cut(s) 345
BstBI TTCGAA 1 cut(s) 586
BstC8I GCNNGC 2 cut(s) 231, 502
BstF5I GGATG 1 cut(s) 66
BstKTI GATC 2 cut(s) 188, 334
BstMAI GTCTC 4 cut(s) 39, 95, 291, 309
BstMBI GATC 2 cut(s) 185, 331
BstMWI GCNNNNNNNGC 5 cut(s) 35, 81, 115, 118, 154
BstNI CCWGG 2 cut(s) 170, 313
BstNSI RCATGY 1 cut(s) 233
BstSCI CCNGG 3 cut(s) 40, 168, 311
BstSFI CTRYAG 1 cut(s) 24
BstSNI TACGTA 1 cut(s) 345
BstV1I GCAGC 6 cut(s) 38, 93, 93, 96, 96, 105
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BtsCI GGATG 1 cut(s) 66
Cac8I GCNNGC 2 cut(s) 231, 502
CseI GACGC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 346, 388
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviAII CATG 2 cut(s) 154, 230
CviQI GTAC 2 cut(s) 346, 388
DpnI GATC 2 cut(s) 187, 333
DpnII GATC 2 cut(s) 185, 331
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
EciI GGCGGA 1 cut(s) 113
Eco105I TACGTA 1 cut(s) 345
Eco57I CTGAAG 1 cut(s) 526
EcoRII CCWGG 2 cut(s) 168, 311
Esp3I CGTCTC 2 cut(s) 39, 95
FaeI CATG 2 cut(s) 157, 233
FaqI GGGAC 1 cut(s) 276
FatI CATG 2 cut(s) 153, 229
FauNDI CATATG 1 cut(s) 242
Fnu4HI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
FokI GGATG 1 cut(s) 73
Fsp4HI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
FspBI CTAG 1 cut(s) 308
GluI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
GsuI CTGGAG 1 cut(s) 384
HapII CCGG 2 cut(s) 41, 428
HgaI GACGC 1 cut(s) 112
Hin1II CATG 2 cut(s) 157, 233
HincII GTYRAC 2 cut(s) 6, 375
HindII GTYRAC 2 cut(s) 6, 375
HinfI GANTC 4 cut(s) 164, 407, 424, 450
HpaII CCGG 2 cut(s) 41, 428
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 2 cut(s) 6, 375
Hpy188I TCNGA 1 cut(s) 185
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 2 cut(s) 6, 375
HpyAV CCTTC 2 cut(s) 50, 269
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4IV ACGT 2 cut(s) 344, 419
HpyCH4V TGCA 3 cut(s) 26, 38, 229
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 81, 115, 118, 154
HpySE526I ACGT 2 cut(s) 344, 419
Hsp92II CATG 2 cut(s) 157, 233
Kzo9I GATC 2 cut(s) 185, 331
LmnI GCTCC 5 cut(s) 133, 209, 359, 365, 562
Lsp1109I GCAGC 6 cut(s) 38, 93, 93, 96, 96, 105
LweI GCATC 1 cut(s) 544
MaeI CTAG 1 cut(s) 308
MaeII ACGT 2 cut(s) 344, 419
MaeIII GTNAC 1 cut(s) 455
MalI GATC 2 cut(s) 187, 333
MboI GATC 2 cut(s) 185, 331
MboII GAAGA 3 cut(s) 313, 316, 532
MfeI CAATTG 1 cut(s) 335
MflI RGATCY 1 cut(s) 185
MluCI AATT 2 cut(s) 335, 351
MlyI GAGTC 1 cut(s) 433
MnlI CCTC 3 cut(s) 25, 373, 538
MseI TTAA 1 cut(s) 576
MslI CAYNNNNRTG 1 cut(s) 234
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 2 cut(s) 41, 428
MspR9I CCNGG 3 cut(s) 42, 170, 313
MunI CAATTG 1 cut(s) 335
Mva1269I GAATGC 1 cut(s) 582
MvaI CCWGG 2 cut(s) 170, 313
MwoI GCNNNNNNNGC 5 cut(s) 35, 81, 115, 118, 154
NciI CCSGG 1 cut(s) 42
NdeI CATATG 1 cut(s) 242
NdeII GATC 2 cut(s) 185, 331
NlaIII CATG 2 cut(s) 157, 233
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 455
NspI RCATGY 1 cut(s) 233
NspV TTCGAA 1 cut(s) 586
PaeI GCATGC 1 cut(s) 233
PctI GAATGC 1 cut(s) 582
PfeI GAWTC 3 cut(s) 164, 407, 450
PkrI GCNGC 7 cut(s) 28, 83, 86, 108, 111, 120, 142
PleI GAGTC 1 cut(s) 432
PpsI GAGTC 1 cut(s) 432
Ppu21I YACGTR 1 cut(s) 345
PsiI TTATAA 1 cut(s) 399
Psp6I CCWGG 2 cut(s) 168, 311
PspGI CCWGG 2 cut(s) 168, 311
PspN4I GGNNCC 1 cut(s) 187
PstI CTGCAG 1 cut(s) 28
PsuI RGATCY 1 cut(s) 185
RsaI GTAC 2 cut(s) 347, 389
RsaNI GTAC 2 cut(s) 346, 388
RseI CAYNNNNRTG 1 cut(s) 234
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
Sau3AI GATC 2 cut(s) 185, 331
SchI GAGTC 1 cut(s) 433
ScrFI CCNGG 3 cut(s) 42, 170, 313
SfaNI GCATC 1 cut(s) 544
SfcI CTRYAG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 586
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
SnaBI TACGTA 1 cut(s) 345
SphI GCATGC 1 cut(s) 233
Sse9I AATT 2 cut(s) 335, 351
SsiI CCGC 3 cut(s) 29, 98, 140
SspMI CTAG 1 cut(s) 308
StyD4I CCNGG 3 cut(s) 40, 168, 311
TaaI ACNGT 1 cut(s) 10
TaiI ACGT 2 cut(s) 347, 422
TaqI TCGA 1 cut(s) 586
TasI AATT 2 cut(s) 335, 351
TatI WGTACW 1 cut(s) 387
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 3 cut(s) 164, 407, 450
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 6 cut(s) 26, 81, 84, 106, 109, 118
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 1 cut(s) 316
TspGWI ACGGA 2 cut(s) 178, 392
XceI RCATGY 1 cut(s) 233
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XspI CTAG 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.